1.Live animal model of cataract for surgical training in phacoemulsification
Sarbani HAZRA ; Dipak K DE ; Arup K BOSE ; Biswarup ROY
International Eye Science 2008;8(6):1091-1094
AIM: To create a live animal model of cataract for training in phacoemulsification.METHODS: The left eye of each dog was selected, and cataract was induced by puncturing the anterior capsule of the lens with the beveled end of the 26 gauge needle under general anesthesia. Phacoemulsification was performed in all the eyes following development of cataract.RESULTS: Ophthalmologic examination showed posterior cortical opacification started from day 5 to 7 after the induction and the complete cataract formation was observed at 75 days post the induction. The phaco-chop technique was practiced in all the dogs, followed by placement of intraocular lens. Vision was restored in all the dogs following the phacoemulsification.CONCLUSION: Considering the enormous losses caused initially by inexperienced surgeons while performing phacoemulsification, we suppose that this live animal model will be far superior to cadaver eyes that are commonly used for practice of phacosurgery.
2.Acute pyogenic bone and joint infections.
Annals of the Academy of Medicine, Singapore 1987;16(2):252-255
Acute infections of bone and joints is a curable condition and the chance of cure is related to a number of factors like type and virulence of the organism, resistance of the host, choice of antibiotics, early drainage of the joint in septic arthritis and appropriate treatment after early diagnosis with adequate dosage and duration of antibiotic therapy. Late diagnosis and inadequate treatment can often lead to high mortality and morbidity and leave the patient with crippling sequelae like chronic osteomyelitis, joint destruction, pain, shortening, deformity and limp.
Acute Disease
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Arthritis, Infectious
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complications
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diagnosis
;
therapy
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Humans
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Osteomyelitis
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complications
;
diagnosis
;
therapy
;
Suppuration
3.Relation of Anti- to Pro-Inflammatory Cytokine Ratios with Acute Myocardial Infarction.
Santanu BISWAS ; Pradip K GHOSHAL ; Sankar C MANDAL ; Nripendranath MANDAL
The Korean Journal of Internal Medicine 2010;25(1):44-50
BACKGROUND/AIMS: Acute myocardial infarction (AMI) is a leading cause of death. Inflammatory processes play an important role in atherosclerosis, which is intimately related to AMI. The aim of this study was to investigate the association between anti-inflammatory and pro-inflammatory cytokines ratios and AMI. METHODS: A total of 90 AMI patients and 90 age-and sex-matched controls were recruited in this study. Plasma cytokines and conventional risk factors were determined by standard methods. RESULTS: Patients with AMI showed increased interleukin (IL)-6 and tumor necrosis factor-alpha levels and lower anti- to pro-inflammatory cytokine ratios as compared with controls. A multivariate logistic regression analysis revealed that IL-10 to IL-6 ratio was independently associated with the occurrence of AMI (odds ratio [OR], 5.39; 95% confidence interval [CI], 2.39 to 12.17; p < 0.0001). In contrast, IL-6 levels were no longer significant in the multivariate model (OR, 1.02; 95% CI, 0.932 to 1.12; p = 0.603). A receiver operating characteristic (ROC) curve analysis indicated that IL-6 levels and IL-10 to IL-6 ratios were a significant predictor of AMI (area under ROC curve, 0.892 and 0.851, respectively). CONCLUSIONS: Our results suggest that the ratio of IL-10 to IL-6 is independently associated with AMI, and reduced levels of this ratio may favor the development of AMI.
Adult
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Atherosclerosis/blood/epidemiology/immunology
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Female
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Humans
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Interleukin-10/*blood
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Interleukin-6/*blood
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Logistic Models
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Male
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Middle Aged
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Multivariate Analysis
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*Myocardial Infarction/blood/epidemiology/immunology
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Predictive Value of Tests
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ROC Curve
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Risk Factors
4.Evaluation of a new flow cytometry based method for detection of BCR-ABL1 fusion protein in chronic myeloid leukemia.
Swati DASGUPTA ; Ujjal K RAY ; Arpita Ghosh MITRA ; Deboshree M BHATTACHARYYA ; Ashis MUKHOPADHYAY ; Priyabrata DAS ; Sudeshna GANGOPADHYAY ; Sudip ROY ; Soma MUKHOPADHYAY
Blood Research 2017;52(2):112-118
BACKGROUND: Philadelphia chromosome, a hallmark of chronic myeloid leukemia (CML), plays a key role in disease pathogenesis. It reflects a balanced reciprocal translocation between long arms of chromosomes 9 and 22 involving BCR and ABL1 genes, respectively. An accurate and reliable detection of BCR-ABL fusion gene is necessary for the diagnosis and monitoring of CML. Previously, many technologies, most of which are laborious and time consuming, have been developed to detect BCR-ABL chimeric gene or chromosome. METHODS: A new flow cytometric immunobead assay was used for detection of BCR-ABL fusion proteins and applicability, sensitivity, reliability, efficacy and rapidity of this method was evaluated. RESULTS: From February 2009 to January 2014, a total 648 CML patients were investigated for the status of BCR-ABL1 protein. Among them, 83 patients were enrolled for comparative study of BCR-ABL1 positivity by three routinely used procedures like karyotyping, and quantitative real time PCR (RT-PCR) as well as immunobead flow cytometry assay. BCR-ABL protein analysis was found consistent, more sensitive (17% greater sensitivity) and reliable than the conventional cytogenetics, as flow cytometry showed 95% concordance rate to RT-PCR. CONCLUSION: BCR-ABL fusion protein assay using a new flow cytometric immunobead might be useful in the diagnosis and monitoring CML patients.
Arm
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Cytogenetics
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Diagnosis
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Flow Cytometry*
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Fusion Proteins, bcr-abl
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Humans
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Karyotyping
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive*
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Methods*
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Philadelphia Chromosome
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Real-Time Polymerase Chain Reaction