1.Subcellular location of HCAP1 by its expression and preparation of antiserum
Juru WANG ; Wenxin QIN ; Jinjun LI ; Al ET
Chinese Journal of Immunology 1985;0(01):-
Objective:To obtain anti HCAP1 polyclonal antiserum and define subcellular localization of HCC associated protein(HCAP1).Methods:The HCAP1 was expressed in E.coli DE3 cells and purified.The antiserum was prepared by immunizing rabbit with purified HCAP1 protein.The expression of HCAP1 was analyzed by Western blot in the HCC cells lines.The subcellular localization was observed using fluoroimmunocytochemistry.Results:The rabbit antiserum was obtained and HCAP1 was localized in cytoplasm and nucleoli.Conclusion:The expressed HCAP1 and anti HCAP1 antiserum could be used for studying the HCAP1 role in the HCC;The expression pattern of HCAP1 was mainly in cytoplasm and it was also detected in the nucleoli.
2.The testing system for OCP of the digital X-ray machine.
Yan WANG ; Guoming MO ; Juru WANG ; Tao ZHOU ; Jianguo YU
Chinese Journal of Medical Instrumentation 2011;35(5):356-360
In this paper, we designed a testing system for operator control panel of a high-voltage and high-frequency X-ray machine, and an online testing software for functional components, in order to help the testing engineers to improve their work efficiency.
Electricity
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Equipment Design
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Man-Machine Systems
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Radiographic Image Enhancement
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instrumentation