Objective To explore the application effects of the construction of the pain management decision and quality monitoring system in big data era.Methods A total of 114 patients who were in Oncology Department ward from January to June 2016 when the system of pain management decision and quality monitoring had not been applied were selected as the control group. After the application of pain management decision and quality monitoring system, from July to December 2016, 132 patients of Oncology Department ward were selected as the observation group. The qualified rate of pain nursing record, the degree of pain relief and pain management satisfaction were statistically analyzed and compared between the two groups. Results After application of pain management decision and the quality monitoring system software, the pain assessment accuracy, the qualified rate of pain nursing records and pain management satisfaction of the observation group increased significantly than those of the control group, and the difference was statistically significant (P<0.05). The degree of pain relief in patients was significantly improved than that before the application, and the difference was statistically significant (P<0.05).Conclusions The application of pain management decision and quality monitoring system promotes the standardization of pain care and improves the efficiency of pain management.

To investigate the effect of nucleoside diphosphate kinase (NDK) on the synthesis of hyaluronic acid, nucleoside diphosphate kinase gene (ndk) was overexpressed along with the hyaluronic acid-producing genes in recombinant B.subtilis. Two engineered strains named Hp8tg and Pn8tg were constructed.Uniform hyaluronic acid (HA) could be obtained from both engineered strains.HA produced by both recombinant strains was confirmed by monosaccharide composition analysis, Fourier transform infrared spectometry and nuclear magnetic resonance spectroscopy.Inducing conditions of HA fermentation were optimized by response surface methodology.Overexpression of ndk could increase the production and molecular weight of HA by 1.3-fold and 1.1-fold, respectively. This study revealed for the first time that overexpression of ndk could relieve the inhibition effect of uridine diphosphate (UDP) on Class II HA synthase and increase the production and molecular weight of HA, which proves to be an efficient strategy for the production of HA, and the preparation of other polysaccharides.