Objective To understand the effect of transforming growth factor-?_(2)(TGF-?_(2)) on the expression and activity of matrix metalloproteinase-2(MMP_(2)) in cultured bovine trabecular cells and to investigate the role of TGF-?_(2) in the pathogenesis of primary open angle glaucoma(POAG). Methods TM cells were incubated in DMEM containing 1.0?g/L TGF-?_(2) or 1.0?g/L TGF-?_(2) with 100mg/L murine monocolonal anti-human PAI-1 IgG or DMEM without any experimental reagent for 24, 36, 48h, respectively. The expression of MMP_(2) or plasminogen activator inhibitor-1(PAI-1) were assayed by Western blot. Results TGF-?_(2 )could enhance the expression of pre-form MMP_(2) and PAI-1. PAI-1 neutralizing antibody could promote transformation of the pre-form MMP_(2) to it's active form. Conclusion TGF-?_(2) can cause the accumulation of ECM in trabecular meshwork by inhibiting the activation process of pre-form MMP_(2), and thus cause the increase of aqueous humor outflow resistance, which contributes to the pathogenesis of POAG.

The cultural development needs of contemporary college students generally are displayed as varied contents,rich types and distinctive characteristics.Students in military medical universities shoulder the responsibilities of both soldiers and medical students,their cultural development needs are often in contradiction to military culture,thus bringing challenges to the culture construction in military medical universities.By introducing activities from civilians and soldiers,constructing hidden cultural platforms,improving hardware facilities and other innovative methods,we can actively guide students' ideological direction and play a better culturally educational function of military schools.

Background The mental health status of prison officers is crucial to the efficiency, security, and stability of a prison, and it is essential to pay attention to the factors that influence their mental health. Objective To understand the mental health status of prison officers, and analyze how nature exposure affects their mental health problems and a potential mediating role of mental fatigue. Methods A cross-sectional survey was carried out from May to June 2022 among 1392 prison officers from eight prisons in a province, and a total of 1284 valid questionnaires were recovered. The Nature Exposure Scale, Mental Fatigue Scale, and Depression-Anxiety-Stress Scale were used to assess nature exposure, mental fatigue, and mental health indicators among prison officers, and to explore the effect of nature exposure on mental health problems and a potential mediating role of mental fatigue. Results The recruited prison officers showed high levels of depression, anxiety, and stress. The prevalence rates of depression, anxiety, and stress were 59.11% (759/1284), 60.67% (779/1284),and 43.93% (564/1284), respectively. The results of correlation analysis revealed that nature exposure was negatively related with mental fatigue and mental health indicators (depression, anxiety, and stress) (r_s=−0.242, −0.308, −0.235, −0.254, P<0.01), while mental fatigue was positively correlated with mental health indicators (depression, anxiety, and stress) (r_s=0.546, 0.533, 0.536, P<0.01). The PROCESS macro results showed that the level of nature exposure among prison officers negatively associated with depression, anxiety, and stress (β=−0.180, −0.104, −0.123), and mental fatigue played a mediating role, with indirect effects of −0.200, −0.192, and −0.199, respectively. Conclusion The levels of depression, anxiety, and stress of prison officers are higher than those of other occupations. Nature exposure negatively associates with depression, anxiety, and stress, that is, it may directly alleviate the mental health problems of prison officers; and it may also alleviate mental health problems by relieving mental fatigue.