Corneal epithelial injury refers to the destruction of corneal epithelial function and structural integrity caused by various reasons.Dry eye is a common cause of corneal epithelial damage and it also delays the healing of corneal epithelial wound.The change of tear film stability is a main mechanism of dry eye disease, which implies an important role of improving the tear film stability in dry eye and the repair of dry eye-related corneal epithelial damage.Ocular surface lubricants can be used to supplement the water-like liquid, mucin and lipid that tears naturally have.Anti-inflammatory drugs can improve the stability of tear film.When the ocular surface microenvironment is affected, the growth factors of the tear are unable to meet the needs of rapid repair of the damage.Therefore, it is necessary to supplement exogenous growth factor to promote injury healing of corneal epithelium.In this article, the repair of corneal epithelial damage associated with dry eye were reviewed, including the therapies of ocular surface lubricant, anti-inflammation and promoting repair of corneal epithelium.

Objective To evaluate the blood-saving effect of tranexamic acid in elderly patients undergoing total hip replacement.Methods One hundred and sixty ASA Ⅱ or Ⅲ patientss of both sexes,aged 65-70 yr,with a body mass index of 16-22kg/m2,undergoing total hip replacement,were randomly divided into 2 groups(n =80,each):control group(group C)and tranexamic acid group(group T).Anesthesia was induced with midazolam,fentanyl,etomidate and atracurium.The patients were tracheal intubated and mechanically ventilated.PEr CO2 was maintained at 35-45 mm Hg.Aneslhesia was maintained with propofol,remifentanil and atracurium.Before the skin incision,tranexamic acid 15 mg/kg was infused over 15 m in in group T,while the equal volume of normal saline was given instead in group C.Hemoglobin(Hb),platelet count(PLT),prothrombin time(PT),and activated partial thromboplastin time(APTT)were monitored during operation to guide blood transfusion.Intraoperative and postoperative blood loss and allogeneic blood transfusion were recorded.Postoperative complications were also recorded.Results There was no significant difference in the amount of intraoperative blood loss between the two groups(P ＞ 0.05).The amount of postoperative blood loss was significantly smaller and less allogeneic red blood cell was transfused in group T than in group C(P ＜ 0.05).No complications occurred after operation in either group.Conchusion Tranexamic acid has blood-saving effect in elderly patients undergoing total hip replacement,but the clinical value is limited.

Objective To explore the correlation between axon guidance factor Semaphorin 5A and clinicopathological features and its role in the invasion and metastasis of gastric cancer.Methods The expression of Semaphorin 5A in gastric cancer tissues of 171 patients with different gender,age,histological type and TNM stage was detected with immunohistochemistry assay.The expression of Semaphorin 5A was determined by Western blotting assay in gastric cancer cell lines SGC7901 and MKN-45 with metastatic ability and gastric cancer cell lines SNU-1 and AGS without metastatic ability.With RNA interfere technique(RNAi),Semaphorin 5A siRNA expression vector was constructed and transfected into gastric cancer cell line SGC7901.The stable gastric cancer cell line down-expressing Semaphorin 5A was established.The effect of Semaphorin 5A gene silencing on the adhesion,migration and invasion of gastric cancer cell was examined by cell adhesion test,wound healing test and transwell chamber assays.Results The expression level of Semaphorin 5A was correlated with the differentiation degree of gastric cancer(x2 =6.32,P =0.01),lymphnode metastasis(x2 =7.68,P=0.01)and distant metastasis of gastric cancer(x2 =13.67,P =0.00),not correlated with age(x2 =0.21,P=0.79),gender(x2=1.79,P=0.15)and the depth of gastric cancer invasion(x2=1.34,P=0.55).The expression of Semaphorin 5A in cell lines SGC7901 and MKN-45 was significantly higher than that of cell lines SNU-1 and AGS(P＜0.01).Semaphorin 5A gene silencing significantly suppressed the adhesion,migration and invasion abilities of gastric cancer cells.Conclusion Semaphorin 5A may play a catalytic role in the invasion and metastasis of gastric cancer through increasing the adhesion,migration and invasion abilities of gastric cancer cell.

Objective:To compare the image quality and the diagnostic efficiency for breast malignant lesions using simultaneous multi-slice single shot echo planar imaging (SMS+SS-EPI) and readout segment of long variable echo trains (RESOLVE) for breast diffusion-weighted imaging (DWI).Methods:This study was a cross-sectional study. Clinical and imaging data of 102 patients with breast lesion from March 2021 to February 2023 in the First Hospital Affiliated to Ningbo University were prospectively analyzed. All patients underwent routine breast MRI scans and cross-sectional RESOLVE and SMS+SS EPI sequence DWI, and the image quality of 2 types sequences of DWI was evaluated. The subjective evaluation was based on a 5-point scale, including geometric distortion, artifact blurring, fat suppression, overall image quality, and lesion conspicuity of the breast. The objective evaluation included signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), and apparent diffusion coefficient (ADC) value of the lesion. Wilcoxon signed rank sum test was used to compare the subjective and objective parameters between the two sequences. Finally, the receiver operating characteristic curve and area under the curve (AUC) were used to evaluate the effectiveness of ADC values for diagnosing breast malignant lesions based on two sequence images.Results:All 102 female patients had single lesions, aged from 25 to 68 years and 60 lesions were malignant and 42 were benign. The acquisition time for SMS+SS-EPI sequence imaging was 1 min 50 s, and the acquisition time for RESOLVE sequence imaging was 3 min 43 s.The subjective scores from both SMS+SS-EPI and RESOLVE sequence were over than 3 points. The geometric distortion, artifact blurring, and overall image quality scores of RESOLVE sequence images were higher than those of SMS+SS-EPI (all P<0.001). The overall lesion conspicuity score, malignant lesion conspicuity score, and benign lesion conspicuity score of DWI and ADC images combined with SMS+SS-EPI sequence were higher than those of the RESOLVE sequence (all P<0.05). There were no statistically difference in SNR, CNR, overall ADC value, malignant ADC value, and benign ADC value between SMS+SS-EPI and RESOLVE sequence DWI images of breast lesions (all P>0.05). The AUC of RESOLVE sequence ADC value in diagnosis of breast cancer was 0.973, the sensitivity was 0.929, and the specificity was 0.915; The AUC of SMS+SSC-EPI sequence ADC value in diagnosis of breast cancer was 0.956, the sensitivity was 0.977, and the specificity was 0.850. Conclusions:In breast DWI, the subjective score of SMS+SS EPI image quality can basically meet the diagnostic requirements and the conspicuity of lesions is better than that of the RESOLVE sequence. Both have good diagnostic efficacy for malignant breast lesions.

As a rapid, accurate and efficient analytical technique, gas chromatography is widely used in the detection of volatile organic compounds and inorganic small molecule toxins, and it is the main analytical method in the national testing standards for occupational health. The existing effective national standards of gas chromatography for the detection of some substances have low column efficiency, high toxicity of reagents, poor correlation of the standard curve and low desorption efficiency and other problems, some of which can be solved through method improvement. At the same time, with the use of new materials and new processes, new types of toxic substances are emerging, and there are still many occupational disease hazards of limited value without supporting detection methods, gas chromatography can be applied to the detection of some toxic substances to better complement the vacancy of China's occupational health detection methods. This paper analyzes the current situation of the application of gas chromatography in occupational health testing standards, discusses the improvement of some of these methods, and helps to promote the application and development of gas chromatography in occupational health testing.

Objective:To use CiteSpace and VOSviewer software to perform visualization analysis on research literature on sudden poisoning incidents in China from 2013 to 2022, in order to grasp the research hotspots and priorities, current research development status and trend of sudden poisoning incidents, and provide reference and ideas for subsequent research.Methods:In October 2023, literature related to sudden poisoning incidents was searched using the China National Knowledge Infrastructure and Wanfang databases. The bibliometric analysis software CiteSpace 6.1.R2 and VOSviewer 1.6.19 were used to visually analyze factors such as authors, institutions, journals, regions, and keywords in the relevant literature.Results:A total of 2578 valid literatures were included, and the overall number of published literatures showed a discontinuous downward trend in recent 10 years. The relevant literature was distributed in 310 journals. Among the top 10 institutions with the highest number of publications, 8 were centers for disease control and prevention at all levels, and the institution with the highest number of publications was the Chinese Center for Disease Control and Prevention (208 articles), but the cooperation between institutions was not obvious. The 2578 articles involved a total of 6549 authors (a total of 8572 appearances, with an average of 1.31 times per person), with a collaboration rate of 2.54 (6549/2578) and 2153 co-authored articles (with 2 or more authors in the literature), with a co-authorship rate of 83.51%. 643 articles (24.94%) received funding support. The region with the highest number of publications was Guangdong Province, with a total of 380 articles (14.74%). Keyword highlighting analysis showed that the research heat of pesticide poisoning, toxic mushrooms, disease characteristics, outbreak events, traceability analysis, outbreaks, and occupational diseases continued to 2022.Conclusion:There are many literatures on sudden poisoning incidents in China, so it is necessary to strengthen the cooperation and communication between institutions and authors. In practical work, the ability of prevention, control, and detection of poisons should be continuously improved to reduce the incidence and degree of harm caused by sudden poisoning incidents.

Objective:To use CiteSpace and VOSviewer software to perform visualization analysis on research literature on sudden poisoning incidents in China from 2013 to 2022, in order to grasp the research hotspots and priorities, current research development status and trend of sudden poisoning incidents, and provide reference and ideas for subsequent research.Methods:In October 2023, literature related to sudden poisoning incidents was searched using the China National Knowledge Infrastructure and Wanfang databases. The bibliometric analysis software CiteSpace 6.1.R2 and VOSviewer 1.6.19 were used to visually analyze factors such as authors, institutions, journals, regions, and keywords in the relevant literature.Results:A total of 2578 valid literatures were included, and the overall number of published literatures showed a discontinuous downward trend in recent 10 years. The relevant literature was distributed in 310 journals. Among the top 10 institutions with the highest number of publications, 8 were centers for disease control and prevention at all levels, and the institution with the highest number of publications was the Chinese Center for Disease Control and Prevention (208 articles), but the cooperation between institutions was not obvious. The 2578 articles involved a total of 6549 authors (a total of 8572 appearances, with an average of 1.31 times per person), with a collaboration rate of 2.54 (6549/2578) and 2153 co-authored articles (with 2 or more authors in the literature), with a co-authorship rate of 83.51%. 643 articles (24.94%) received funding support. The region with the highest number of publications was Guangdong Province, with a total of 380 articles (14.74%). Keyword highlighting analysis showed that the research heat of pesticide poisoning, toxic mushrooms, disease characteristics, outbreak events, traceability analysis, outbreaks, and occupational diseases continued to 2022.Conclusion:There are many literatures on sudden poisoning incidents in China, so it is necessary to strengthen the cooperation and communication between institutions and authors. In practical work, the ability of prevention, control, and detection of poisons should be continuously improved to reduce the incidence and degree of harm caused by sudden poisoning incidents.

As a rapid, accurate and efficient analytical technique, gas chromatography is widely used in the detection of volatile organic compounds and inorganic small molecule toxins, and it is the main analytical method in the national testing standards for occupational health. The existing effective national standards of gas chromatography for the detection of some substances have low column efficiency, high toxicity of reagents, poor correlation of the standard curve and low desorption efficiency and other problems, some of which can be solved through method improvement. At the same time, with the use of new materials and new processes, new types of toxic substances are emerging, and there are still many occupational disease hazards of limited value without supporting detection methods, gas chromatography can be applied to the detection of some toxic substances to better complement the vacancy of China's occupational health detection methods. This paper analyzes the current situation of the application of gas chromatography in occupational health testing standards, discusses the improvement of some of these methods, and helps to promote the application and development of gas chromatography in occupational health testing.

Objective To develop a diagnostic model based on deep neural network for intelligent discrimination of thyroid function. Methods A total of 1616 patients ( 283 males, 1333 females, average age:52 years) who underwent thyroid imaging between May 2016 and June 2018 were selected. According to the clinical diagnosis, the 1616 cases included 299 normal thyroid cases, 876 hyperthyroidism cases and 441 hypothyroidism cases. Feature extraction and learning training were performed on 1000 training set sam-ples by two deep neural network models ( AlexNet;deep convolution generative adversarial networks ( DCGAN) ) using deep learning algorithm. Performance verifications were implemented on 616 test set samples. The con-sistency between the verification results of the two models and the clinical diagnosis was analyzed by Kappa test. Meanwhile, the time advantage of the intelligent diagnosis models was analyzed. Results The average diagnostic time of AlexNet model was 1 s/case, and the classification accuracy for normal thyroid, hyperthy-roidism, hypothyroidism were 82.29％(79/96), 94.62％(369/390), 100％(130/130), respectively. The Kappa value between results of AlexNet model and clinical diagnosis was 0.886 ( P<0.05) . The average di-agnostic time of DCGAN model was 1 s/case, and the classification accuracy for normal thyroid, hyperthy-roidism, hypothyroidism were 85.42％(82/96), 95.64％(373/390), 99.23％(129/130), respectively. The Kappa value between results of DCGAN model and clinical diagnosis was 0.904 ( P<0.05) . Conclusion The deep neural network intelligent diagnosis model can quickly determine the functional status of thyroid gland in thyroid imaging, and it has a high recognition accuracy, thus providing a new method for thyroid image review.

Background Propylene glycol monomethyl ether (PGME) is a widely used organic solvent. It exists in the form of two isomers in the workplace, which will cause adverse effects such as eye and upper respiratory tract irritation in workers. However, there is still a lack of standard detection methods for simultaneous detection of two isomers of PGME in China. Objective To establish a solvent desorption-gas chromatographic method for two isomers of PGME [1-methoxy-2-propanol (α-PGME), 2-methoxy-1-propanol (β-PGME)] in workplace air. Methods A method of solvent desorption-gas chromatography for α-PGME and β-PGME in workplace air were proposed. Air samples were collected with solvent desorption activated carbon tubes, desorbed using a desorption solution of dichloromethane/methanol (85:15), and then separated on a free fatty acid phase (FFAP) fused silica capillary chromatography column , detected with a flame ionization detector (FID), and quantified by peak area. Standard evaluation protocol was followed to obtain key indicators: standard curve, limit of detection, lower limit of quantification, relative standard deviation (RSD) that measures precision, and spiked recovery of sample solutions that measures accuracy. Desorption efficiency, sampling efficiency, and adsorption capacity tests were conducted, sample stability was evaluated using spiked activated carbon tube preservation test, and interference test was also assessed. The developed method was then applied to field air sample testing. Results In this method, using dichloromethane/methanol (85:15) as the sample desorption solution, the quantitative detection ranges of α-PGME and β-PGME were 0.95-923.0 μg·mL⁻¹ and 0.97-912.0 μg·mL⁻¹ with both correlation coefficients of 0.999 9, the method limits of detection were 0.28 µg·mL⁻¹ and 0.29 µg·mL⁻¹, and the lower limits of quantification were 0.95 µg·mL⁻¹ and 0.97 µg·mL¹, respectively. The lowest concentrations detected were both 0.19 mg·m⁻³, and the lowest concentrations quantified were 0.63 mg·m⁻³ and 0.65 mg·m⁻³, respectively, under the conditions of sampling volume of 1.5 L and the volume of desorption solution of 1.0 mL. The intra-batch precisions for α-PGME and β-PGME were 2.8%-4.9% and 2.8%-5.1%, the inter-batch precisions were 4.2%-5.7% and 4.5%-5.9%, the spiked recoveries were 98.8%-100.3% and 96.4%-102.9%, and the desorption efficiencies were 92.7%-97.3% and 92.2%-98.1%, respectively. The average sampling efficiency was 100%, and the samples could be stored at room temperature (25-30 ℃) for at least 7 d and at 4 ℃ for at least 15 d. The adsorption capacities of the activated carbon sampling tube for α-PGME and β-PGME were greater than 13.9 mg and 2.7 mg (100 mg of activated carbon adsorbent), respectively. Possible co-existing components in workplace did not interfere with the determination of α-PGME and β-PGME. The short-time exposure concentrations of α-PGME and β-PGME in the spray painting unit of an automobile manufacturing company were determined to be 18.69 mg·m⁻³ and 2.19 mg·m⁻³, and the time-weighted average concentrations were 6.03 mg·m⁻³ and 1.08 mg·m⁻³ respectively. Conclusion This method is accurate, precise and suitable for on-site monitoring of the two isomers of PGME in workplace air.