The effects of acupuncture, massage and herbs on cerebral parlsy of children were reviewed based on evidence in the current study. It was proved that traditional medicine was eddective in treating cerebral parlsy of childred.

Fifty cases of acute lumbar sprain were treated by the combination of acupuncture, cupping and kinesitherapy. Forty-three cases were cured and 7 cases improved, the total effective rate being 100%. This therapy has an exact effect with a short course of treatment.

Apoptosis ; Fatty Liver, Alcoholic ; Humans ; Lipid Metabolism

Objective To observe the effect of Gongxuening for the women placing the IUDafter cesarean section, and to analyze the effect and value of Gongxuening on intrauterine device placement after cesarean section. Methods 156 women who received voluntary intrauterine device after cesarean sectionin theShengzhou Maternal and Child Health Hospital of Shaoxing, Zhejiang were as the observation object. According to whether the application of Gongxuening, they were divided into two groupsafter treatment, the Gongxuening group of 75 cases (using Gongxuening + Nifloxacin) and the control group of 81 cases (using norfloxacin). The changes of serum inflammatory indexes were compared between the two groups before and after operation, and the uterine bleeding was compared between the two groups. Results After operation, the levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10) were with a significantfluctuation, and at the same time, Gongxuening group TNF-α, IL-6, IL-10, 24hours after surgery, postoperative 7d was significantly lower than the control group, the difference was statistically significant (P< 0.05). After treatment, the WBC of Gongxuening group 24hours after surgery, postoperative 7d was significantly lower than the control group, but the Hb 24 hours after surgey was significantly higher than the control group, the difference was statistically significant (P<0.05). The bleeding volume2h, 24h, bleeding stop time and puerperium duration in the Gongxuening group were significantlylower than the control group, the difference was statistically significant (P<0.05). Conclusion After cesarean section maternal IUD use Gongxuening capsule treatment, not only can effectively inhibit the expression of serum inflammatory markers, and can shorten the time of uterine bleeding, reduce bleeding, prevent postoperative anemia. It is suggested to pay more attention to clinical treatment.

Drug delivery system in tumor targeting research has always been a difficult problem.Apoptosis in vitro experiments and body pharmacokinetic experiments can not reflect the targeting of drug delivery system indirectly.Imaging techniques both in vivo and in vitro solve the problem,researchers can not only observe the drug delivery system in the target organ,the target tissue and target cell directly,but also analyze quantitatively and qualitatively.

[Objective]To discuss the effect of femoral head diameter on risk of dislocation after primary total hip arthroplasty,in order to choose the reasonable diameter of femoral head for preventing the postoperative dislocation. [Methods]From March 1990 to August 2008,primary total hip arthroplasties with various head sizes were performed in 706 patients(762 hips).There were 301 males and 405 females.The average age of the 706 patients in this series were 57 years(range 21～78 years).The preoperative diagnosis for each of the patients were osteonecrosis in 203,congenital dysplasia in 129(the Crowe IV in 15),femoral neck fracture in 190,osteoarthritis in 112,rheumatoid arthritis and ankylosing spondylitis in 58 and the other diagnosis in 14.The femoral head diameter was 22mm in 220 of the procedures,26mm in 83,and 28mm in 459.Among the 762 cases,a modified Harding approach was preferred in 95%,and a posterolateral approach was preferred in 5%.Patients routinely were followed at definite intervals and were specifically queried about dislocation.[Results]All the patients were followed up for at least six months.Of the 762 cases,there were 28 dislocations;the dislocation rate was 3.7%.In the subgroups,there were 7.3%(16/220) for 22mm heads,4.8%(4/83) for 26mm heads,and 1.7%(8/459) for 28mm heads,the difference were statistically significant(x2=13.193,P=0.001;R=0.131,P=0﹤0.01).[Conclusion]The femoral head diameter has a positive effect on risk of dislocation,using a larger femoral head could reduce the risk of dislocation after total hip arthroplasty.A larger femoral head could be selected for preventing the postoperative dislocation and 22mm femoral heads should be avoided.

To investigate the significance and age characteristics of CVB infections among common diseases of children.CVB Ag and CVB IgM were detected from the sera in 967 children in hospital. Positive rates of CVB Ag and CVB IgM in the upper respiratory tract infection group, the lower respiratory tract infection group, the myocarditis group, the asthma group, the ITP group and the anaphylactoid purpura group were higher than the control group.Most of the children with positive CVB were in the 0～1 year age group and 1～3 years age group had respiratory tract infection. With increasing age,the number of the children with respiratory tract infection gradually decreased,while number of the children with myocarditis,asthma,ITP and anaphylactoid purpura increased in those children with positive CVB. It suggests that, beside respiratory tract infection,CVB plays an important role in pathogenesis of myocarditis,asthma,ITP and allergic purpura in children.

Objective To compare the features of cerebral palsy with pachygyria or periventricular leukomalacia (PVL). Methods 15 children with pachygyria and 35 children with PVL were included. Their diagnosed age, clinical subtype, complications, Gross Motor Func-tion Classification System (GMFCS) were compared. Results The diagnosed age was significantly younger (P<0.001), the GMFCS was lower (P<0.05), the incidence rate of epilepsy was higher (P<0.05), and the intelligence was significantly lower (P<0.001) in the pachygyria group than in the PVL group. Conclusion Cerebral palsy children with pachygyria develope worse than that with PVL, as well as their prog-nosis.

As an effective method of studying soluble protein-protein interactions, yeast display system is now widely used for affinity maturation of single-chain antibodies.Due to the strong homology recombination machinery of yeast and the high-throughput nature of FACS detection, a rapid scan for interaction between antigen-antibody pairs could be easily achieved.Based on this system, a novel and reliable method for determining conformational epitopes was developed.Different fragments of macrophage migration inhibitory factor(MIF) and several point mutations of MIF were displayed on yeast cell surface using homologous recombination technology.Three MIF monoclonal antibodies, 10C3, 2A12 and 4E10, were screened for their binding affinity to each displayed peptide.Utilizing this technology, the key amino acids of MIF that bind to the MIF monoclonal antibodies were easily identified.