To investigate the significance and age characteristics of CVB infections among common diseases of children.CVB Ag and CVB IgM were detected from the sera in 967 children in hospital. Positive rates of CVB Ag and CVB IgM in the upper respiratory tract infection group, the lower respiratory tract infection group, the myocarditis group, the asthma group, the ITP group and the anaphylactoid purpura group were higher than the control group.Most of the children with positive CVB were in the 0～1 year age group and 1～3 years age group had respiratory tract infection. With increasing age,the number of the children with respiratory tract infection gradually decreased,while number of the children with myocarditis,asthma,ITP and anaphylactoid purpura increased in those children with positive CVB. It suggests that, beside respiratory tract infection,CVB plays an important role in pathogenesis of myocarditis,asthma,ITP and allergic purpura in children.

Pain, as one of the most common clinical symptoms, seriously affects the quality of life of patients.Transient Re-ceptor Potential Vanilloid Type 1 (TRPV1) and Purinergic receptor(P2X3)are both involved in the transmission of a variety of patho-logical pain signals.Their interaction is less reported.This article reviews the interaction between TRPV1 and P2X3 in the pain signal transduction, and provides a new way for the treatment of pain.

Objective To study the drug susceptibility,potential toxin factors,and genotypes of Candida albicans.Methods Forty-six strains of C.albicans were isolated from clinical samples of lower respiratory tract infection.The genomic DNA was amplified by PCR to determine the 25S rDNA genotypes.The drug sensitivity was investigated by disk diffusion and broth dilution respectively.Adhesiveness test and protease activity assay were performed.Results Forty-six strains were divided into A(18),B(6),and C(22) three genotypes.Most trains displayed high sensitivity to natamycin,amphotericin B,nystatin,fluorocytosine,and itraconazole.MIC results showed that there was significant difference against fluconazole and 5-fluorocytosine between A and B as well as C genotypes(P0.01).The cell adhesiveness test demonstrated that the strain adhesiveness was proportional to the protease activity(r=0.977,P

Cystic liver metastasis, which lacks specific clinical manifestations and standardized treatment, rarely occurs after radical gastrectomy. This study presented two gastric cancer cases with cystic liver metastasis treated in Wuxi People's Hospital from September 2008 to November 2012. The two female patients were 66 and 65 years old, respectively. Poorly differentiated ulcerated adenocarcinoma classified as stage III B [Tumor, Node, and Metastasis Classification (T4aN2M0)] and clinical manifestations of abdominal distension and ascites were detected in both patients. Cyst fluids were subjected to cytological analysis. Results confirmed the presence of liver metastasis of gastric cancer. The two patients benefited from local intracavitary chemotherapy or systemic intravenous chemotherapy.

Objective To discuss the necessity and feasibility of nucleic acid test(NAT)in clinical blood transfusion by the im-plementation of the nucleic acid testing after the ELISA screening of Zhengzhou blood donors.Methods HBV DNA,HCV RNA, HIV RNA were detected by Roche Cobas S201 system and Shanghai kehua screening system,the samples were mixed by 6×166.7μL and 8×180 μL(as one pool)separately.If the mix pool was negative,the result can be issued directly;if the pool was positive, than secondary single sample dectection must be taken and the secondary report was the final result.Results A total of 115 227 blood samples were screened by Roche Cobas S201 system and 130 mix pools were positive,among which 80 pools were reactive by secondary split testing,and the reactive samples were 86,the split ratio was 61.5%,The positive ratio of specimens was 0.75‰.90 359 samples were screened by kehua system,and the mixed pools were 93,among which 31 pools were reactive by secondary split testing,and the reactive samples were 31,the split ratio was 33.4%,The positive ratio of specimens was 0.34‰.So the total num-ber screened by the two systems was 205 586,among which 117 cases were reactive,the total positive ratio of specimens was 0.57‰.And one case was HIV window phase infection.Conclusion NAT could effectively decrease the risk of blood transfusion caused by omission of ELISA and ensure the safety of blood transfusion.

To meet the demands of educating high-quality talents with broad basic medical knowledges background,strong operating skills and good innovative consciousness,we attempted to reform the conventional teaching models of clinical microbiology laboratory as following:①to optimize the teaching contents;②to enhance links between laboratory technologies and medicines;③to improve undergraduate students'examinations and evaluation systems.The teaching effect gets the recognition of students and peers.

Animals ; Bone Neoplasms ; Bone and Bones ; Humans ; Models, Animal ; Neoplasms ; Pain

OBJECTIVETo observe the intervention of electroacupuncture (EA) with different current frequencies and treatment frequencies on pain thresholt in rats with bone-cancer pain, so as to optimize treatment parameters of EA against bone cancer pain; and by measuring gene expression of opioid receptor and precursor in different tissues to preliminarily explore the possible mechanism of EA against bone cancer pain.

METHODSNinety healthy female SD rats were randomly divided into a control group, a model group, EA groups (6 subgroups according to different frequencies) and a sham EA group, ten rats in each one. Rats in the control group were injected with 10 µL of amicrobic phosphate buffer solution (PBS) into tibial cavity; rats in the remaining groups were injected with Walker 256 cancer cells to establish model of bone-cancer pain. No treatment was given to rats in the control group and model group; rats in the EA groups were treated with EA at bilateral "Housanli" (ST 36) and "Genduan" with 3 different current frequencies (2 Hz, 100 Hz and 2 Hz/100 Hz), once a day and once every other day, 30 min per treatment (1mA for 15 min, 2 mA for 15 min); rats in the sham EA group were treated with identical acupoints as the EA group, but the acupoints were needled subcutaneously and EA was connected with power off. All the treatment was given for 14 days. Dynamic plantar aesthesiometer was applied to measure the paw withdrawal thresholds (PWTs) of the affected side before the model establishment, 6d, 8d, 10d, 12d, 14d, 16d, 18d, and 20d after model establishment. The mRNA expressions of µ-opioid receptor (MOR), κ-opioid receptor (KOR), δ-opioid receptor (DOR), proopiomelanocortin (POMC) and prodynorphin (PDYN) in dorsal root ganglion (DRG) and lumbar spinal cord dorsal horn (SCDH) of L4-L6 of the affected side were detected by PCR method.

RESULTSThere were no differences in PWTs among all groups before model establishment (P>0. 05). Each time point after model establishment, PWTs in model group were obviously lower than those in the control group (all P<0. 01). Compared with the model group, PWTs in each EA subgroup were all increased (all P<0.05), but the differences at different time points were not significant among EA subgroups (P>0.05). The mRNA expressions of MOR, KOR, POMC, and PDYN in L4-L6 DRG in the 2 Hz/100 Hz II group were significantly higher than those in model group (P<0. 05, P<0. 01), while the mRNA expressions of MOR, KOR, DOR, POMC and PDYN in SCDH were not different compared with the model group (P>0. 05).

CONCLUSIONEA treatment has obvious analgesic effect on bone-cancer pain, however, its effect is not related with current frequency and treating frequency. EA against bone-cancer pain may be related with increasing the mRNA expression of some peripheral opioid receptors and precursor.

Acupuncture Analgesia ; instrumentation ; methods ; Acupuncture Points ; Animals ; Bone Neoplasms ; complications ; Electroacupuncture ; instrumentation ; methods ; Enkephalins ; metabolism ; Female ; Ganglia, Spinal ; metabolism ; Humans ; Pain ; etiology ; genetics ; metabolism ; Pain Management ; instrumentation ; methods ; Protein Precursors ; metabolism ; Rats ; Rats, Sprague-Dawley ; Receptors, Opioid ; genetics ; metabolism

Postoperative ileus (POI) is a common abdominal postoperative complication of surgery as well as obstetrics and gynecology. There is a lack of an effective method of modern medicine due to its complex pathophysiological mechanism and the postoperative physiological disorder of patient. Acupuncture has remarkable regulatory effects on gastrointestinal function. Some clinical studies indicated that acupuncture was an effective method to treat POI, which could reduce the duration of POI and the treatment costs of patients in hospital. However, the mechanism and law of acupuncture on treating POI is still unclear. Some clinical studies indicated that the regulatory effect of acupuncture on the gastrointestinal motility was associated with its regulation of the autonomic nerve system and immune system. Based on its effect on regulating autonomic nerve system and immune system, acupuncture would be a potential and safe treatment for POI.

Objective To observe the functional changes of T lymphocytes in the spleen of rats with bone cancer pain.Methods forty-one healthy female Sprague-Dawley rats were used in this study, and were divided into blank control, PBS and Walker-256 tumor groups.Bone cancer pain model was established by inoculation of Walker 256 cancer cells into the tibial cavity.The paw withdrawal threshold (PWT), paw withdrawal thermal latency (PWL), and spontaneous pain (SP) were all measured before modelling (as base) and at 4, 6, 8, 10, 12, 14, 16, 18, and 20 days after modelling. The function of T lymphocyte proliferation, and the content of T lymphocytes and their subgroups in the spleen were detected by cell counting kit-8 method and flow cytometry, respectively, on day 20 after modelling.Results Before modellng, there were no differences of PWT, PWL, and SP between the PBS and model groups.After modelling, the PWT and SP of model group were significantly decreased on day 4, and were always lower than that of PBS group during the experiment.Statistical analysis revealed that Walker-256 cancer cell inoculation in the tibia induced a significant decrease in PWL on day 8, 10 and 12 after modellng.Compared with the control group, T lymphocyte proliferation, content of T lymphocyte (CD3) and subgroups ( CD4 and CD8) in the PBS group were not significantly decreased.However, T lymphocyte proliferation and the content of CD3 lymphocytes in the model group were significantly lower than those in the blank control group and/or PBS group.Conclusions The bone cancer pain rat model may appear obvious mechanical allodynia and spontaneous pain.Its thermal pain hyperalgesiaonly occurred in the intermediate stage of bone cancer pain. The content of T lymphocytes and its subgroups, and the function of T lymphocyte proliferation are weakened to some extent in the bone cancer pain rat model.