The complement system is a part of the innate immune system that potentiates the ability of antibodies and phagocytic cells to clear microbes and damaged cells. The complement system consists of a number of proteins circulating as inactive precursors. It is stimulated mainly by three pathways: the classical pathway, the alternative pathway, and the lectin pathway. There are many genetic polymorphisms in this system, which can over-activate the immune system. In this study, we collected the polymorphisms reported to over-activate complement cascades that affect the immune system and induce autoimmune diseases.
Antibodies ; Autoimmune Diseases ; Complement System Proteins* ; Immune System ; Phagocytes ; Polymorphism, Genetic*

To identify the interchangeability of V(H) and V(L) framework region (FR) residues, we artificially introduced random mutations at all residue positions in a chicken monoclonal antibody, which has only one functional V(H) and Vλ gene. When we classified the amino acids into 5 groups by their physicochemical properties, all FR residues could be replaced by another group except L23 (C), H36 (W), H86 (D), H104 (G), and H106 (G). Eighty-two (50.9%), 48 (29.8%), 17 (10.6%), and 9 FR residues (5.6%) could be replaced by 4, 3, 2, and 1 group(s), individually, without significant loss of reactivity. We also confirmed a similar level of versatility with 2 different chicken antibodies. This high level of versatility on FR residues has not been predicted because it has not been observed in the 150 chicken antibodies that we previously generated or in the 1,269 naïve chicken V(H) sequences publically available. In conclusion, chicken antibody FR residues are highly interchangeable and this property can be applied for improving the physicochemical property of antibody including thermal stability, solubility and viscosity.
Amino Acids ; Antibodies ; Chickens* ; Immunoglobulin Variable Region ; Solubility ; Somatic Hypermutation, Immunoglobulin ; Viscosity

Primary epiploic appendagitis (PEA) is a condition that results from spontaneous torsion, ischemia, or inflammation of an epiploic appendage. This condition is manifested by localized abdominal pain and tenderness, which is often mistaken for diverticulitis or appendicitis in the adult population. PEA is a self-limiting disease, and differential diagnosis with surgical condition is important in order to prevent unnecessary surgery. Although it is commonly reported in adults, it is rarely reported in the pediatric population. We report on the radiologic findings of two cases of PEA in a 12- and a 17-year-old boy, focusing on sonographic findings.
Abdominal Pain ; Adolescent ; Adult ; Appendicitis ; Child* ; Diagnosis, Differential ; Diverticulitis ; Humans ; Inflammation ; Ischemia ; Male ; Peas ; Pediatrics ; Ultrasonography ; Unnecessary Procedures