Objective To evaluate the incidence of hyponatremia in patients with Guillain-Barré syndrom (GBS) and to explore whether hyponatremia is an important predictor of poor outcome.Methods All 455 GBS patients met the inclusion criteria and were divided into three groups as mild,moderate and severe GBS according to Medical Research Council sumscore (MRC sumscore) at nadir.Hyponatremia is defined as serum sodium concentration below 135 mmol/L at nadir.The incidences of hyponatremia in GBS patients with different MRC sumscore were analyzed by x2 tests.Logistic regression models were performed to determine risk factors of hyponatremia and poor prognosis.Results Of all the 455 GBS patients,mild,moderate and severe groups patients were 178,100 and 177 respectively,and 98 (21.5％) GBS patients had hyponatremia.Hyponatremia was more often detected in severe GBS cases (64,36.2％),than in mild (21,11.8％) or moderate (13,13.0％,x2 =28.921,17.074 respectively; all P ＜ 0.01) cases,but there was no significant difference between mild and moderate patients (x2 =0.086 ; P =0.769).Logistic regression analysis identified 3 predictors of hyponatremia:age ＞ 50 (OR =2.013,95％ CI 1.155-3.508,P =0.014),facial weakness (OR =2.493,95％ CI 1.423-4.369,P =0.001) and mechanical ventilation (OR =12.871,95％ CI 5.484-30.207,P =0.001).The presence of hyponatremia (OR =12.770,95％ CI3.419-47.701,P =0.001) and bulbar weakness (OR =3.391,95％ CI 1.182-9.733,P =0.023)predicted death in acute stage.Conclusion As an important predictor of poor outcome in GBS patients,hyponatremia is more likely occurred in patients with older age,facial weakness or respiratory failure.

C. Conclusion Adequate hemodilution can attenuate the lung injury induced by I/R. The protective effect is better if hemodilution is performed before I/R.

BACKGROUND:Adipose-derived stem cel s and bone marrow mesenchymal stem cel s are used widely in cartilage tissue engineering, and there are many similarities in biological characteristics between two kinds of cel s. OBJECTIVE:To compare the chondrogenic potential of bone marrow mesenchymal stem cel s and adipose-derived stem cel s in vitro. METHODS:Adipose-derived stem cel s were isolated from the 3-month-old New Zealand white rabbits’ abdomen. Bilateral femurs of rabbits were obtained, and then the bone marrow mesenchymal stem cel s were separated with the adherence screening method. The growth curve of the passage 3 adipose-derived stem cel s and bone marrow mesenchymal stem cel s were drawn, and the doubling time of two kinds of cel s was compared. Then the passage 3 adipose-derived stem cel s and bone marrow mesenchymal stem cel s were treated with chondrogenic induction. After induced for 14 days, the adipose-derived stem cel s and bone marrow mesenchymal stem cel s were treated with toluidine blue staining and type Ⅱ immunohistochemistry staining respectively. RESULTS AND CONCLUSION:Primary bone marrow mesenchymal stem cel s showed aggregative growth, while the primary adipose-derived stem cel s were in single and scattered growth. The proliferation speed of adipose-derived stem cel s was faster than that of bone marrow mesenchymal stem cel s, while the doubling time of adipose-derived stem cel s was shorter than that of the bone marrow mesenchymal stem cel s. After chondrogenic induction for 14 days, both adipose-derived stem cel s and bone marrow mesenchymal stem cel s could express glycosaminoglycans and type Ⅱcol agen, and the expression level of type Ⅱ col agen in bone marrow mesenchymal stem cel s after chondrogenic induction was higher than that in the adipose-derived stem cel s. The in vitro proliferation of adipose-derived stem cel s and bone marrow mesenchymal stem cel s was rapid and stable, but the proliferative ability of adipose-derived stem cel s was faster than that of bone marrow mesenchymal stem cel s. When cultured in single layer, both adipose-derived stem cel s and bone marrow mesenchymal stem cel s could transform into chondrocytes under certain conditions, but bone marrow mesenchymal stem cel s seemed to be more potential than adipose-derived stem cel s.

Through the consultation of TCM ancient classical theory, the relationship of kidney essence, marrow and brain is analyzed. It is discovered that the degenerative brain diseases, represented by Alzheimer's disease (AD) and Parkinson's disease (PD) share the same etiological basis as "kidney essence deficiency and brain marrow emptiness" and have the mutual pathological outcomes as yang qi declining. The Governor Vessel gathers yang qi of the whole body and maintains the normal functional activity of zangfu organs in the human body through the storage, regulation and invigoration of yang qi. It is viewed that the theory of the Governor Vessel is applied to treat the different degenerative brain diseases, which provides the theoretic support and practice guide for the thought of TCM as the same therapeutic program for the different diseases. As a result, the degenerative brain diseases can be retarded and the approach is provided to the effective prevention and treatment of degenerative diseases in central nerve system:
Acupuncture Points ; Acupuncture Therapy ; Alzheimer Disease ; therapy ; Humans ; Meridians ; Parkinson Disease ; therapy ; Qi

Objective To establish a method for lysis coagula rapidly within endometriosis cyst to ease aspiration difficulty while ultrasound-guided puncture and aspiration therapy. Methods Urokinase for injection with different concentration was used to lysis the coagula within endometriosis ovarian cyst with thick content. The effect of urokinase on the coagula, the wall of cyst and the aspiration rate of content were investigated. Results The area of coagula in unit area was significantly small in urokinase group compared with that in control group[( 95.6? 17.5)?m2 vs ( 1692.3? 1028.3)?m2,F= 28.86,P= 0.000]. Urokinase can lysis the coagula appending to the wall but did not change the permeability of cyst wall. The aspiration rate was significantly faster in urokinase group than in control group[( 2.79? 2.61)ml/min vs ( 1.42? 0.58)ml/min; t= 2.4572,P= 0.0121]. Conclusions Urokinase can resolve the coagula within endometriosis ovarian cyst and on the cyst wall safely and effectively, making it easy for cyst content aspirating and improving the coagulation effect of ethanol.

OBJECTIVE: To establish a new pattern of ward round record for clinical pharmacist and rational use of drug. METHODS: The new pattern of ward round record was established according to domestic and foreign history pattern, The Principle and Proposed Pattern of Clinical Medication History in China, disease course record. This new pattern focused on patients’ disease and medication changes. A case of cardiovascular department was taken as example. RESULTS: Established ward around record contained two parts, i.e. first page and record chart. CONCLUSION: Established pattern is concise and practical and covers essential medical information. It makes communication more easily between pharmacist and doctors and meets the needs of clinical pharmacy practice.

AIM:To study the chemical constituents of the root of Myrica nana cheval.METHODS:The constituents were separated and purified by different methods of chromatography,and their structures were elucidated by UV,IR,GM and NMR.RESULTS:Seven compounds were isolated from the root of Myrica nana cheval.Their structures were identified as ?-sitosterol(1),myricanol(2),myricanone(3),ursolic acid(4),quercetin(5),myricetin(6)and myricitrin(7).CONCLUSION:Except for 5,6 and 7,the other compounds were obtained from this plant for the first time.

Ultrasound-based molecular imaging technique is a role in positioning based on the conventional ultrasonic detection technology in combination with the targeted ultrasound contrast agent.It is a new technology to detect the changes of target tissue molecular level in vivo state.This article reviews the principles of ultrasound-based molecular imaging and its application in the evaluations of early inflammatory response,neovascularization,and vulnerable plaque in carotid atherosclerosis.

Objective To investigate the effects of Wuling Powder on insulin resistance of C57BL/6J mice induced by high lipid diet, and discuss the mechanism. Methods C57BL/6J mice were randomly divided into six groups:normal group, model group, rosiglitazone group, Wuling Powder in low, middle, and high dose groups, 10 mice per group. Besides the normal group, other five groups were fed with high fat and sugar diet, with a purpose to establish insulin resistance model. Normal group and model group were given pure water. Rosiglitazone group received a gavage with rosiglitazone of 0.75 mg/kg. Wuling Power low, middle, and high groups received gavage with Wuling Power of 1.23, 3.69, 11.07 g/kg, respectively, the does volume was 0.2 mL/10 g, once a day. The weight and abdominal girth were detected every week. At the end of the sixth week, mice were given 12-hour fasting, and their eyeball were taken for blood. The body weight, length, and fat in abdomen and both kidneys were detected. Paraffin section was made with HE staining. FPG and FINS of each group were detected. ISI and IRI were calculated, and TC and TG were detected. Results Compared with the model group, Wuling Powder can significantly reduce the body weight and abdominal girth of mice (P<0.01, P<0.001), improve liver fatty degeneration, lower the FPG, FINS, TCH, TG, IRI, and increase the ISI in mice (P<0.05, P<0.01). Conclusions Wuling Powder has the effect of preventing insulin resistance of C57BL/6J mice induced by high lipid diet.

BACKGROUND:Animal experiments have repeatedly verified that umbilical cord stem celltransplantation can improve the rotational behavior of rats with Parkinson’s disease, with lower immune rejection response.
OBJECTIVE:To evaluate the therapeutic effects of human umbilical cord mesenchymal stem celltransplantation in the treatment of Parkinson’s disease using the Unified Parkinson’s Disease Rating Scale.
METHODS:Fifteen patients with Parkinson’s disease were enrol ed, including 8 males and 7 females, aged 52-76 years. Hoehn&Yahr staging was 3-5. After informed consent was obtained from puerperae and the procedure was approved by the hospital ethics committee, ful-term maternal umbilical cord was aseptical y col ected, to culture umbilical cord mesenchymal stem cells. Al patients were hospitalized, and treated with human umbilical cord mesenchymal stem celltransplantation via carotid artery puncture. Neurological function of patients was assessed using a comprehensive rating scale for Parkinson’s disease before and 1 month after cells transplantation. Higher score indicated more severe neurological deficits.
RESULTS AND CONCLUSION:Fifteen patients entered the result analysis. Compared with before transplantation, 15 patients showed significantly lowered scores on the Unified Parkinson’s Disease Rating Scale at 1 month after transplantation (P<0.05). The improvement was mainly concentrated in the tremor and rigidity, but bradykinesia and unstable position were not improved. Graft versus host disease did not occur in al 15 cases. These findings indicate that umbilical cord blood mesenchymal stem celltransplantation for treating Parkinson’s disease has obvious curative effects and significantly improves neurological functions.