Purpose:To study the clinical response and the toxic-side reactions of patients with gastric cancer treated with continuous infusion of fluorouracil (5-FU) lasting 240 hours.Methods:35 cases of gastric cancer were treated with continuous infusion of 5-FU lasting 240 hours.32 cases of gastric cancer were treated with traditional infusion of 5-FU on days 1 to 5. The clinical response and the toxic -side reactions were observed.Results:35 cases were treated with continuous infusion of 5-FU regimen with a response rate of 51.4%. 32 cases were treated with traditional infusion of 5-FU regimen with a response rate of 25.0%,thus between them,there was a significant difference in curative effects. The toxicities in traditional infusion of 5-FU group were more severe than in continuous infusion of 5-FU group. They included myelosuppression nausea et al.Conclusions:Continuous infusion of 5-FU regimen is preferable in the treatment of gastric cancer.

ObjectiveTo investigate the effect of Smad7 antisense oligodeoxynucleotide (ASODN) on proliferation in human pancreatic cancer cell line SW1990,with a focus on the expression of matrix metalloproteinase-2(MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2).To explore the underlying mechanism of the role of Smad7 in the pathogenesis and development of pancreatic cancer.MethodsSmad7 ASODN was transfected into SW1990 cells through lipofectamine.Nosense oligodeoxynucleotide (NSODN),ASODN and lipofectamine was used as control. The transfection efficiency was assessed by fluorescence microscopy and flow cytometry.The expressions of Smad7,MMP-2 and TIMP-2 in transfected cells were detectedby RT-PCR and Western blot.Cell viability was assessed by dimethyl thiazoldiphenyltetrazoliumbromide (MTT) method. Results Smad7 was expressed in SW1990 cells.The transfection efficiency of SW1990 was 81.2％.The expressions of Smad7 mRNA were 0.34 ± 0.06,0.95 ±0.07,1.03 ± 0.11 in transfected group,ASODN and lipofectamine group; and the expressions of MMP-2 mRNAwere 0.54 ± 0.08,1.15 ± 0.13,1.27 ± 0.16 ; and the expressions of TIMP - 2 mRNA were 0.26 ±0.07,0.72 ± 0.13,0.78 ± 0.17,the mRNA expressions were significantly reduced in Smad7 ASODN transfected group,compared with other two groups (P ＜0.01 ).The expressions of Smad7 protein were 0.14 ± 0.03,0.29 ± 0.05,0.28 ± 0.07 in transfected group,ASODN and lipofectamine group; the expressions of MMP-2 protein were 0.17 ±0.02,0.29 ±0.05,0.31 ±0.04,and the expressions of TIMP-2 protein were 0.20 ± 0.03,0.41 ± 0.11,0.43 ± 0.09,the protein expressions were significantly reduced in Smad7 ASODN transfected group,compared with other two groups (P ＜0.01 ).The A490 values of proliferation were 0.83 ± 0.03,1.02 ±0.02,0.99 ±0.02 in transfected group,ASODN and lipofectamine group,the proliferation were significantly reduced in Smad7 ASODN transfected group,compared with other two groups (P ＜0.01 ).ConclusionsSmad7 ASODN could effectively inhibit the expressions of Smad7,therefore decrease the expressions of MMP-2,TIMP-2 and reduce the proliferation.

Objective:To establish a soft tissue 3-dimension(3D)finite element model of human palate.Methods:Based on the intrinsic finite element model of human maxilla,palatal mucoperiosteum flap with the thickness of 3.06 mm was added on the oral side of the plate model by ANSYS software,material property of the soft tissue was loaded,the soft tissue 3D finite element model of the palate was established.Results:By applying finite element method,the established 3D finite element model could reflect biomechani-cal properties of the soft tissue of the palate on computer.Conclusion:The 3D finite element model of the soft tissue of human palate provides the foundation for further study.

Objective:To study the impact of palatal scar force on the maxilla with unilateral cleft palate by finite element model. Methods:A 3-dimension finite element model of maxilla with cleft palate containing soft tissue was established,the soar force on the maxilla was analyzed by ansys.Results:Cleft area was identified as the center of rotation,cleft side of the maxilla reflected clock-wise rotation and non-cleft side anticlockwise rotation.The maxilla moved from outside to inside in cross section and front to back in the sagittal plane,and the former was greater than the latter.The outside of alveolar process was subjected to stress of mainly drawing force,the inside to stress of mainly compressive force.The deformation of maxilla was mainly in the front-end.Deformation of the alveolar was greater than that of maxilla body.Among deformations of three dimensions,the width was the largest,the length was the smallest,and the height was between both above.Conclusion:Along with the rotation of the maxilla,cleft area becomes gradually smaller.Among the three dimensions,the width is the largest,the length is the smallest and the height is between the both.The af-fect on maxilla is mainly in the front-end.The affect on the alveolar is greater than that on the maxilla body.

In a hospital, key disciplines are often regarded as the representative of service and reputation of the hospital. It is therefore of great significance to strengthen the construction of key disciplines to ensure sustainable development of the hospital in the increasingly fierce competition of medical market. This paper discusses how to strengthen construction of key disciplines from four aspects, namely, to increase the awareness of focal discipline construction among hospital staffs, and establish "brand awareness" ;to develop a team of high level professionals, and develop or introduce academic leaders; to improve academic research level and promote the technology progress ;and to strengthen management by objectives.

AIM: To explore the therapeutic effect of malleolus radialis implantation with vascularized periosteal flap for disunion of carpal navicular fracture, and investigate the characteristics of tissue implantation. METHODS: Thirty-two patients with disunion in carpal navicular fracture were selected from Jiangyin People's Hospital from January 2000 to January 2006, with the informed consents of all the patients. They were treated with implantation of malleolus radialis with vascularized periosteal flap, fixed with plaster cast for 8-10 weeks. Therapeutic effect was evaluated as: ①excellent: normal activity of carpal joints, without soreness sense, fracture line disappeared by X ray;②fine: uncomfortable activity of carpal joints, with slight limitations, fracture line disappeared by X ray. RESULTS: ①All 32 cases of the disunion in carpal navicular fracture were synostosis displayed by X ray.②There were 23 cases followed up for 3-12 months, 7 cases for 13-24 months, and 2 cases for 25-36 months. Carpal joints function achieved the standard of the uninjured side, without pain or affection on daily life.③The therapeutic effect was excellent in 22 cases, fine in 10 cases, and good rate was 100%. CONCLUSION: Implantation of malleolus radialis with vascularized periosteal flap is an effective way to treat the disunion of carpal navicular fracture.

To investigate chemical constituents from Radix Pittospori, chloroform extract of the roots was subjected to column chromatography with various chromatographic techniques. The structures were elucidated on the basis of physico-chemical property and spectral analysis. Two triterpenoids were identified as 22-acetyl-21-(2-acetoxy-2-methylbutanoyl)-R1-barrigenol(1) and 3alpha-hydroxyl-20-demethylisoaleuritolic-14(15)-ene-28, 30-dioic acid (2). Compound 1 is a new triterpene and compound 2 is isolated from this plant for the first time.

Neoadjuvant therapy combined with surgery is a very promising clinical treatment strategy for esophageal cancer. Combined with its effectiveness and safety, neoadjuvant radiotherapy can be used as a treatment method for severe esophageal cancer and unresectable esophageal cancer. Neoadjuvant chemotherapy has a higher value in the treatment of adenocarcinoma. Neoadjuvant cheoradiotherapy can be used as the recommended treatment scheme for patients with squamous cancer.

Immonocheckpoint inhabitors have become the focus of tumor therapy in recent years, and more and more tumor patients benefit from immunotherapy. Due to the high cost of immunotherapy, the benefit rate of immunotherapy for untested population is only 20%. Therefore, accurate selection of predictive biomarkers is crucial for individualized immunotherapy of tumor patients. Biomarkers reflecting tumor immune microenvironment and tumor cell intrinsic features, such as programmed death-1 (PD-1) and its ligand PD-L1, tumor mutational burden and microsatellite instability, have been proved to associate with treatment effect of anti-PD-1/anti-PD-L1 therapy. At the same time, markers based on tissue and serum emerge in endlessly. How to truly achieve accurate immunotherapy for tumor needs further clinical research.

With the development of immunotherapy in clinical application, immunotherapy also takes advantage in esophageal squamous cell carcinoma (ESCC). Immune checkpoint inhibitors such as programmed death-1 (PD-1) and its ligand PD-L1 and cytotoxic T lymphocyte antigen-4 show significant antitumor activity and safety in immunotherapy for patients with advanced ESCC.