Objective To study the HPLC fingerprint methodology of Rupikang Capsules. Methods The precision, stability and repeatability of the fingerprint of Rupikang Capsules were researched by HPLC. The HPLC system:Agilent ZORBAX SB-C18 column (4.6 mm×250 mm, 5μm);the mobile phase:water-0.1% phosphoric acid water-acetonitrile, with linear gradient elution;the flow rate:1 mL/min;the detection wavelength:280 nm.Results The precision, stability and repeatability of Rupikang Capsules met the requirements of HPLC fingerprint technology. Conclusion This method is simple, feasible and can be used as the whole quality control method of Rupikang capsules.

Objective To establish the quality standard for Rupikang Capsules. Methods Lumbricus were identified by TLC. Tanshinone IIA was determined by HPLC. HPLC analysis was carried out on Hitachi L-2000 C18 column (250 mm×4.6 mm, 5 μm) and the mixture of methanol-water (75∶25) was used as the mobile phase, the flow rate was 1.0 mL/min and the detection wavelength was at 270 nm. Results TLC spots developed were fairly clear and the blank test showed no interference. The linear range was 0.16-0.4 μg for tanshinone ⅡA, r=0.999 9. The average recovery was 99.1%, RSD=2.20%. Conclusion The methods is easy to operate, with good specificity and reproducibility. It can be used for the quality control of Rupikang Capsules.

Objective: To examine the parents’rearing and educating patterns and their relationship to the characteris- tics of self- concept of adolescents. Methods: A sample of 313 adolescents was tested by the Self- concept Scale and the revised EMBU. Results: Physical self and character self of boys are higher than those of girls. Family self of adolescents whose father has high education level is higher than that of adolescents whose father has low education level(P

Objective:To examine the difference of undergraduates' gender belief in sex and gender role stereotype.Methods:A sample of 552 undergraduates was tested by BSRI and gender belief stereotype scale.Results:①Traditional gender belief of academic,occupation and family has difference on sex and gender role stereotype.②Men college students have higher traditional gender belief of academic,occupation and family than women college students.③College students of masculinity and undifferentiation have higher traditional gender belief of academic and occupation than college students of androgynous and femininity.College students of masculinity have higher traditional gender belief of family than college students of femininity,androgynous and undifferentiation.Conclusion:College students of men and masculinity have higher gender belief of academic,occupation and family.

AIM: To determine the contents of hesperidin and salvianolic acid B in Xiaopikang Capsule(Folium Citri reticulatae,Radix et Rhizoma Salviae miltiorrhizae,etc.) by HPLC. METHODS: The HPLC system consisted of Diamonsil C_(18) column(250 mm?4.6 mm,5 ?m), metanol-acetonitrile-formicacid-water(30∶10∶1∶59) mixture as mobile phase,detection wavelength at 284 nm,flow rate of 1.0 mL/min. RESULTS: The ca-(libration) curves of two ingredients were linear within the ranges of 0.22 ?g-2.2 ?g(r=0.999 6,hesperidin),(1.62 ?g-10.2 ?g)(r=0.999 9,salvianolic acid B),respectively.The average recoveries of hesperidin was(98.9%)(RSD=1.60%),salvianolic acid B for 99.02%(RSD=0.97%). CONCLUSION: The method is rapid,sensitive and accurate.

Objective To evaluate systematically the effect of ventilator circuit changes on incidence of ventilator-associated pneumonia(VAP).Methods Literatures about the effect of ventilator circuit changes on occurrence of VAP were searched from PubMed,Cochrane Library,CNKI,VIP,and Wanfang database,incidence of VAP in pa-tients who received circuit changes at different intervals was compared,odds ratio (OR)and 95%CI were as effect index of the study,RevMan 5.2 software was used for data analysis.Results A total of 12 literatures were included,including 18003 patients using ventilators.According to the interval of ventilator circuit changes,patients were divided into 1-day group (n=191),2-day group(n=8932),3-day group(n=228),and 7-day group(n=8652).Meta analysis showed that circuit changed every 7 days had a lower risk of VAP than that changed once ev-ery day (OR,3.72[95%CI,1.50,9.23]),there was no significant difference in risk of VAP between 7-day group and 2-day group (OR,1.17[95%CI,1.00,1.37])as well as 7-day group and 3-day group(OR,0.77 [95%CI, 0.49,1 .23]).Conclusion Patients who received circuit changes every 7 days have a lower risk of developing VAP than those who received circuit changes once per day,so change the ventilator circuit in patients with mechanical ventilation every 7 days is more reasonable.

OBJECTIVE To find out some risk factors of lower respiratory tract infection in neurology(department) and provide controlling measures.METHODS Totally 883 inpatient cases of neurology(department) in 2005 were retrospectively surveyed and statistically analyzed.RESULTS From them 98 cases were with nosocomial(infection).The infection rate was 11.1%.Among the 98 cases,54 cases were with respiratory tract infection(55%).Lower respiratory tract is the popular part of nosocomial infection in(neurology) department.(CONCLUSIONS) The(treatment) and recovery of the patients are heavily influenced by the infection of lower(respiratory) tract.It is(necessary) to control and prevent main risk factors in order to avoid nosocomial infection.

Objective To observe the changes and its significance of the levels of serum cytokines——interleukin-(IL)1?,IL-6,tumor necrosis factor(TNF)-? and C-reactive protein(CRP) in patients with cerebral infarction(CI).Methods The serum IL-1?,IL-6 and TNF-? level were detected by radioimmunnoassay and serum CRP level by immunoturbidimetry in 30 CI patients with DM(DMCI group),30 CI patients without DM(NDMCI group),20 patients with DM(DM group) and 20 normol controls(NC group).The situation of CI patients were assessed by modified Edinburgh-Scandinavian stroke scale(ESSS).Results The serum levels of IL-1? [(0.60?0.04)ng/ml,(0.33?0.03)ng/ml,(0.30?0.02)ng/ml],IL-6 [(231.07?7.68)pg/ml,(141.34?6.50)pg/ml,(118.92?5.82) pg/ml],TNF-?[(2.70?0.11)ng/ml,(1.85?0.11)ng/ml,(1.21?0.13) ng/ml] and CRP [(7.44?0.26),g/L,(4.67?0.21)mg/L,(4.54?0.24)mg/L] in DMCI group,NDMCI group and DM group were significantly higher than those in NC group [(0.20?0.03)ng/ml,(60.99?5.98)pg/ml,(0.70?0.10) ng/ml,(3.83?0.14) mg/L](all P

Objective:To explore the factors influencing colonization of Mutans streptococci (MS) on teeth of children.Methods:Dental colonization of MS was investigated in 123 children aged from 7 to 42 months in Beijing. Oral examination was performed. Information of nursing habits, diet history etc. was obtained from parent questionnaires.Results:Logistic regression analysis showed that the number of erupted teeth and the intake of sugar-containing food were positively related with MS colonization, their WALD values were 17.411(P=0.000) and 5.637(P=0.018) respectively,OR values were 3.287 and 2.739 respectively.Conclusion:The main factors influencing the colonization of MS were the number of erupted teeth and the intake of sugar-containing food.

Objective To investigate the possibility of measuring ABO blood group antibody levels using renal cortical tubular epithelial cells (RCTECs) of cynomolgus monkey.Method The primary RCTECs were isolated from cynomolgus monkey kidneys and identified by Western blotting and flow cytometry (FACS).FACS was applied to detect the expression of ABO histo-blood group antigens on RCTECs,the binding of blood group antibodies in human serum to RCTECs,and to compare the difference of measuring ABO blood group antibody levels between using human erythrocytes and RCTECs as target cells.Result The majority of cells derived from the kidney cortex were renal tubular epithelial cells.39.90％-73.80％ of RCTECs were found to express the ABO histoblood group antigens with the capability to bind the human blood group antibodies specifically.The expression level of ABO histo-blood group antigen on RCTECs was not influenced by long-term cryopreservation,and the percentage of B antigen positive cells was 39.90％ before frozen storage and 39.65％ after a 10-month cryopreservation respectively.The normal serum and allergic serum presensitized to synthetic A antigen-keyhole limpet hemocyanin (KLH-A) of cynomolgus monkey were used to compare the abilities of RCTECs and human erythrocytes to detect antibody titers.No agglutination was observed in RCTECs test group.The mean fluorescence intensity (MFI) values of antibody were highest when the dilution fold was lowest (1 ∶ 16) and gradually decreased with increased serum dilution in both serum groups.MFI fell towards baseline value at 1 ∶ 128 dilution in normal serum group while at 1∶8 192 dilution in allergic serum group.Between 1 ∶ 16 and 1∶8 192 dilutions,MFIs of normal serum group were all lower than those of allergic serum.In human red cells test group,obvious agglutination appeared at high concentrations of antibodies,and MFIs reached the peak at 1∶64 dilution in normal serum group and at 1∶32 dilution in allergic serum group,but fluctuated irregularly thereafter.Between 1∶64 and 1∶512 dilutions,MFIs of normal serum group were all higher than those of allergic serum.Data above showed that MFI values in RCTECs group could reflect the levels of blood group antibodies more exactly.Conclusion The RCTECs of cynomolgus monkey express ABO tissue-blood group antigens and would be more suitable to be used to measure ABO blood group antibody levels by FACS.