Objective To compare the curative effect of quadruple therapy and triple therapy for Helicobact-er pylori positive peptic ulcer.Methods 62 patients with Helicobacter pylori positive peptic ulcer were randomly devided into observation group(32 cases)and control group(30 cases).Patients in the control group were treated with triple therapy (lansoprazole,amoxicillin,clarithromycin),while the observation group was given quadruple therapy (lansoprazole,amoxicillin,clarithromycin,colloidal bismmth pectin).The clinical efficacy,ulcer healing rate,H.pylori eradication rate and adverse reaction rate of the two groups were observed and compared.Results The clinical effect of the observation group was better than the control group(remission rate 96.88% vs.83.33%),the difference was significant (χ2 =21.44,P <0.05).The H.pylori eradication rate in the observation group was higher than that in the control group (93.75% vs 76.67%)(χ2 =49.56,P <0.05).The ulcer healing rate in the observation group was higher than that of the control group(P >0.05).The incidence of adverse reactions between the two groups had no statistically significant difference (P >0.05).Conclusion Quadruple therapy in the treatment of H.pylori positive peptic ulcer has good curative effect,did not increase the incidence of adverse reactions.It is worthy of popularization and application.

OBJECTIVE: To optimize the extraction condition of volatile oil from five medicinal materials in Rutongxiaojie granules and to optimize the inclusion process of the extracted volatile oil. METHODS: The extraction condition was evaluated by orthogonal test with the recovery of volatile oil as indicator; and the inclusion condition was evaluated with the comprehensive scores of recovery ratio of inclusion compound, the content of volatile oil in inclusion compound and the utilization ratio of the volatile oil as criteria. RESULTS: The optimum extraction condition was established as follows: the medicinal materials were soaked in 10 times volume of water for 1hour and distilled for 9h by steam distillation. The optimum inclusion condition was as follows: the proportion of volatile oil to ?-CD was 1∶8; the inclusion temperature was 30℃ and including time was 20min. CONCLUSION: Both the extraction condition and the inclusion condition were reasonable and feasible.

Objective To study the morbidity rate of and relevance to coronary stenosis in cerebral infarction patients. Methods CT coronary angiography was performed in 112 cases of cerebral infarction after CT cerebral angiography. Multivariate logistic regression analysis was carried out between the clinical data and coronary stenosis. Results In 112 cases receiving CT cerebral angiography, the morbidity rate of coronary stenosis was 46.4%. In 95 cerebral infarction patients, the morbidity rate of coronary stenosis was 51.6%. Multivariate logistic analysis showed that age, hypertension, hyperlipeidemia, significant narrowing of cerebral artery were identified as independent predictors for coronary stenosis. Conclusions Heart examination with 64 row CT should be routinely performed after cerebral angiography in cerebral infarction patients,especially in those with age greater than 65 years, hypertension, hyperlipoidemia and significant narrowing of cerebral artery so as to detect coronary stenosis early.

ct curative effect and prognosis of multiple myeloma.

This study was aimed to explore the antitumor effect of volatile oil of X ihuang pill and its immune mecha-nism in order to screen the antitumor active site of Xihuang pill. Among 70 female Wistar rats, 10 rats were random-ly selected as the blank control group; and the other 60 rats was used in the establishment of walker 256 breast can-cer cell tumor-bearing rat models. The model rats were randomly divided into the negative control group (model group), high-dose volatile oil group, middle-dose volatile oil group, low-dose volatile oil group, high-dose Xihuang pill group, and lentinan group (positive control group), with 10 rats in each group. The intragastric administration was given twice a day for 14 days. Blood was taken from the abdominal aorta. Tumor tissues was removed and weighed to calculate the tumor inhibitory rate. ELISA method was used to detect the level of IL-2, IL-6, IL-10, IFN-γ and TGF-β in peripheral blood. The flow cytometry was used to detect the content of CD3+ T cell, CD4+ T cell, CD8+ T cell, and B7-1 cell (CD80). The results showed that the tumor inhibitory rates of volatile oil of high-dose group and middle-dose group were 28.4% and 24.1%, respectively. Compared with the model group, the average level of IL-2 and IFN-γ of volatile oil of high-dose group and middle-dose group and CD3+ T cell, CD8+ T cell, B7-1 cell con-tent were obviously increased (P< 0.05). It was concluded that volatile oil of Xihuang pill had certain antitumor ef-fect, which was one of the antitumor active sites of Xihuang pill. The volatile oil of Xihuang pill upregulates the lev-els of IL-2 and IFN-γ, as well as the contents of CD3+ T cell, CD8+ T cell, B7-1 cell in order to increase the im-mune clearance function of tumor-bearing rats.

Objective To screen and identify effective small interfering RNA (siRNAs) targeting the conserved 5’ untrans-lated region (UTR) of the enterovirus 71 (EV71) genome. Methods Double-stranded siRNAs were designed to target the 5’UTR of the EV71 genome. The cytotoxicity effect of siRNAs on rhabdomyosarcoma (RD) cells was evaluated. The cytopathic effect on EV71-infected RD cells was observed under phase-contrast microscopy and the effective siRNAs were screened out by cell viability assay and real-time TaqMan RT-PCR assay. Results All the siRNAs did not exhibit any cytotoxic effects on the viability and growth of RD cells. Transfection of si-1 and si-2 targeting the 5’ UTR in EV71 genome into RD cells signiifcantly delayed and alleviated the cytopathic effects of EV71 infection, increased cell viability and reduced the EV71 RNA transcripts. And the inhibitory effect of si-1/si-2 on EV71 replication was sequence-speciifc. Conclusions The 2 siRNAs (si-1 and si-2) targeting the conserved 5’UTR of the EV71 genome may have broad spectrum antiviral effects on many epidemic EV71 strains.

Patent documentation is a kind of high-value scientific literature, which has caught the attention of more and more scholars. In this paper, the World Traditional Medicine Patent Database (WTMPD) was used to retrieve patents of the traditional medicines used in the treatment and prevention of influenza worldwide in the past 30 years. A total of 2 423 patents were further screened by a series of processes. Finally, multi-angle analysis was carried on the dataset to form a patent analysis report on traditional anti-flu drugs. From the analysis, we found that China had a rapid increase in the number of patent applications, which was in the leading role in the world, though the number of PCT applicant from China is relatively low. A large amount of applicants from the USA, Japan, and Korea have entered into China. Pharmaceutical company is the core group of patent applicant, who had applied a lot of quality patent about their products to protect their commercial interests. Honeysuckle, licorice, isatis root, forsythia and skullcap are five kinds of herbs, which have been most frequently used in anti-influenza patents.

Aim To construct a eukaryotic expression vector of human MCHR2 and to analyze the stable expression and signal transduction pathways of MCHR2 in CHO cells. Methods The full-length MCHR2 cDNA fragment was amplified by PCR from the human fetal brain cDNA library and inserted into eukaryotic expres-sion vector pcDNA3.1(+), resulted in the recombinant expression vector pcDNA3.1-MCHR2. The recombinant plasmid was confirmed by the restriction enzymatic digestion and DNA sequencing analysis, and then transfected into CHO cells by lipofectamine. A stably-transfected cell line was obtained by the dominant G418 selection, and the expression of the MCHR2 gene on transcription and translation levels were identified by RT-PCR and Western blot. Signal transduction pathways mediated by MCHR2 were analyzed by measurement of intracellular cAMP and calcium. Results The eukaryotic expression vector pcDNA3.1-MCHR2 was successfully constructed and the MCHR2 gene was stably transfected into CHO cells. A stably-transfected cell line was established and the MCHR2 gene was efficiently transcribed and translated. MCH stimulation had no effect on the production of cAMP, however, it could induce a clear and transient increase of intracellular calcium, suggesting that MCHR2 was only coupled to Gq protein. Conclusion The stable expression of MCHR2 and analysis of its signal transduction pathways in CHO cell line provided a solid experimental foundation for further studies on the function of the MCHR2 gene in vitro.

Objective To investigate the possible role of oxidative stress in the protection of hydrogen sulfide during renal ischemia reperfusion.Methods Male Wistar rats were randomly divided into 3 groups: sham operation (Sham) group, renal ischemia reperfusion (IR) group subject to occlusion of left renal pedicle for 45 min then reperfusion for 24 h, and sodium hydrosulfide (NaHS) preconditioning group with continuous infusion of NaHS (450 nmol/min) by left renal artery for 10 min before ischemia reperfusion.Renal injuries were evaluated by PAS staining.The protein levels of NADPH oxidase (NOX) 4, NOX2 were analyzed by Western blotting.The reactive oxygen species (ROS) level of renal tissue was determined by dihydroethidium (DHE) staining assay.Renal superoxide dismutase (SOD), malonic dialdehyde (MDA) and Scr, BUN were evaluated by chromatometry assay.Cell apoptosis were evaluated by TdT-mediated dUTP nick end labeling (TUNEL) staining.Results Compared with Sham group, in IR group the renal NOX4 and NOX2 protein expressions, the existence of acute tubular necrosis and ROS expression were up-regulated (all P ＜ 0.01);MDA, Scr, and BUN were increased and SOD was decreased significantly in IR-treated kidney (all P ＜ 0.01);Moreover, more apoptotic cells presented in the risk zone of IR-treated kindey (P ＜ 0.01).The effects induced by IR were inhibited by NaHS.Compared to that in IR group, NaHS precondition reversed IR-induced damages of renal function and renal tissue, increased SOD activity and decreased MDA expression (all P ＜ 0.05), as well as reduced the expression of NOX4, NOX2 and ROS (all P ＜ 0.05).Moreover, NaHS precondition reduced apoptosis after IR (P ＜ 0.05).Conclusions NaHS alleviates renal ischemia reperfusion injury through inhibiting oxidative stress.Hydrogen sulfide can decrease ROS by inhibiting the activation of NOX, further inhibit the activation of NOD-like receptor, and alleviate kidney damage.

Objective To determine the results of treatment combining all-trans-retinoic acid(ATRA)in childhood acute promyelocytic leukemia(APL).Methods 22 children with newly diagnosed APL received induction therapy with ATRA followed by 3 courses of consolidation chemotherapy:daunorubicin,idarubicin,homoharringtonine or aclacinomycin plus cytosine arabinoside.A maintenance therapy was then administered with ATRA and these reigems for 36 months.Results Early deaths from diffuse intravazcular clotting and intracranial hemorrhage occurred in two patients.The other children achieved a complete remission(CR).By June 2007,the estimated disease-free survival rates at 1,3 and 5 years were 100%,93.3% and 84.7%;respectively.The side effects of ATRA were xerosis eutis and xerocheilia,headaches,nausea and vomiting,hepatic function lesion and ATRA syndrome.Conclusion Remission induction therapy with ATRA is effective and safe for newly diagnosed childhood APL.The maintenance therapy combined chemotherapy with ATRA can improve the long-term effects of APL patients.The main causes of death in APL children is diffuse intravascular clotting and intracranial hemorrhage.The side effects of ATRA can be tolerated.