Objective To provide a new method for measuring cervical spine curvature and the range of flexion-extension and to analyse the radiographic features of the cervical degeneration.Methods The study included 78 patients with cervical spondylosis,90 patients with neck symptoms and 86 normal volunteers.The three groups were compared with each other by the indexes of the cervical spine curvature and the range of flexion-extension.Results There was significantly difference among three groups in the cervical vertebrae lordosis at neutral position.The range of flexion-extension of cervical spondylosis in the patients was smaller than that of the normal volunteers.Conclusions The index method can accurately depict the cervical spine curvature and the range of flexion-extension.There is definitive order in symptom appearance,alterations of physiologic curvature and flexion-extension in the cervical degeneration.

Objective To investigate clinical effect of limited decompression under endoscope combined with percutaneous transpedicular screw fixation for treatment of degenerative lumbar spinal stenosis.Methods Thirty-four senile patients with lower lumbar spinal stenosis were selected as our subjects from February 2010 to August 2011,and were treated with limited decompression under endoscope combined with percutaneous transpedicular screw fixation through posterior approach.The VAS scores of all patients were collected at the four periods including pre-operation,leaving hospital,3 months after operation,and the last follow-up.The therapeutic effectiveness for the postoperative follow-up was expressed as excellent,good,ordinary,and poor according to Nakai standard.Results The VAS scores at pre-operation,leaving hospital,3 month after operation,and the last follow-up were (7.9 ± 1.6),(2.9 ± 1.4),(1.9 ± 1.9),(2.4 ± 1.5)respectively,and the difference was significant (F =13.524,P =0.036).But no significant differences of VAS scores at three sequential tests after operation were observed (P ＞ 0.05).In the last postoperative follow-up,32 cases were graded as excellent,1 cases for good and 1 case for ordinary,and the excellent rate reached to 97％.Conclusion The therapeutic effectiveness of lumbar spinal stenosis limited decompression under endoscope combined with percutaneous transpedicular screw fixation is reliable.Furthermore,no side effects on spine and back muscles are observed.

OBJECTIVE:To control the drug varieties in our hospital. METHODS:According to Prescription Administrative Policy,the related meetings was hold by Drug Therapy and Pharmacy Administration Committee and monitoring procedure was set up to ensure drug elimination publicly,fair and clearly. Drugs of different specifications with same general name were limited strictly. RESULTS:After implementing drug elimination measure,drugs reserved were in line with national drug control standard. CONCLUSION:Drug elimination applied to control drug varieties can improve drug management in the hospital.

AIM: Tissue engineered periosteum fabricated by porcine small intestinal submucosa (SIS) combined with rabbit bone marrow-derived mesenchymal stem cells (BMSCs), are similar to the natural periosteum in the morphology, structure and function. The study was designed to explore the cell attachment in process of constructing tissue-engineered periosteum by different wetting methods to determine the most effective method of fabricating tissue-engineered periosteum. METHODS: The experiment was carried out in the Institute of Orthopedics, the Second Affiliated Hospital of Lanzhou University from September 2005 to March 2007.①Two New Zealand purebred rabbits, 20 days old, were used in this study.②Bone marrow was obtained from healthy rabbits, and BMSCs were isolated by using density gradient centrifugation for culture in vitro. After induction, BMSCs were seeded to SIS wetted with fetal bovine serum (experimental group) and pretreated with phosphate buffered saline (control group), to construct the tissue-engineered periosteum that was cultured for two weeks in vitro. ③The adhesion rates were calculated, and alkaline phosphatase (ALP) activity was detected after seeding. The growth, dissociation and proliferation of the cells were observed by inverted microscope and scanning electron microscope. RESULTS: ①At 5 hours and 12 hours after seeding, the adhesion rates were higher in the experimental group than in the control group [(57.34?2.23)%, (39.16?5.22)%, P

The paper reviewed the history and characteristics of the public hospital reform in China. Based on the theories of corporate governance, it made analysis and comments on existing roadblocks and reform attempts in such hospitals, proposing a public hospital governance model in line with the general reforms made in China's healthcare system sector.

The effects of amino acid electuary on experimental liver fibrosis in rats with chronic liver injury induced by CCI4 were studied. The results showed that the electuary could prevent the decrease in body weight and food intake, reduce the levels of serum glutamic-pyruvic transaminase activities, elevate contents of total serum protein and serum albumin, normalize the serum ratio of BCAA to AAA and disordered amino acid pattern and suppress degeneration, necrosis and fibrosis of liver tissues in rats with chromc liver injury. These results indicate that the amino acid electuary has beneficial effects in prevention and treatment of liver fibrosis.

The effects of amino acid electuary (Ganan Elemental Granules) on experimental acute hepatic failure induced by D-galactosamine in rats were studied. The results showed that the electuary could significantly prevent the decrease in body weight and food intake, the prolongation in time of blood coagulation time and increase in the levels of serum glutamic-pyruvic transaminase activity, elevate contents of total serum protein and serum albumin, normalize the serum ratio of BCAA to AAA and disordered amino acid pattern, increase survival of animals and improve encephalopathy in rats with acute hepatic failure. These results suggest that the amino acid electuary has a better protective effects on acute hepatic failure.

AIM: To establish a new human endometial carcinoma cell line for basic and clinical study. METHODS: The minced tumor tissues were inoculated subcutaneously into the back of nude mice. After forming solid tumor, the mice were killed and the transplant was taken out for primery culture. RESULTS: The established cell line was maintained by serial passages, named EAC. EAC cell line grew rapidly,steadily, and it had similar microscopic morphology to adenocarcinoma cell. Chromosome analysis reveal EAC chromosome counts ranging in hyperdiploid. The expression of ER, PR, P53, MDM2 were negative by SP immunohistochemistry. Tumorigenicity of EAC in nude mice was 100%. CONCLUSION: A new human endometrial carcinoma, EAC, is established successfully, which is helpful for the basic and clinical study of endometrial carcinoma.

Objective To study the clinical topography of lateral ligament of the rectum in the relation to surgical procedures in rectal carcinoma. Method Twenty-three pelvises(12 males,11 females)harvested from embalmed cadavers were studied by topographic dissection. Results The lateral rectal ligamem were identiffed bilaterally in all cadavers between the rectum and visceral fascia.Unilateral middle rectal arteries was found in 8 cadavers and bilateral arteries was found in 2 cadavers.The rectal branches from the pelvic plexus were uniformly constant structure within lateral ligament of the rectum.Conclusion The lateral rectal ligament is located between rectum and visceral fascia.The cleavage between visceral fascia and pelvic plexus is the appropriate plane for lateral rectal dissection of rectal cancers.

Objective To study the level of vascular endothelial growth factor(VEGF)in perioperative period of acute myocardial infarction (AMI)patients received percutaneous coronary intervention (PCI),and to analyze the predictive value of VEGF level for AMI diagnosis and revascularization after PCI.Methods From January 2015 to January 2017,90 AMI patients in Rizhao Central Hospital were selected as the observation group.During the same period,50 healthy subjects were selected as the control group.The changes of VEGF level in the observation group before and after PCI were observed and compared with those in the control group.According to PCI postoperative revascularization,the observation group was divided into blood supply complete group (A group)and blood supply incomplete group (B group),the serum VEGF levels were compared between the two groups after operation.Results The VEGF levels in the observation group before PCI,immediately after operation,2d after operation,7d after opera-tion were (249.2 ±32.1)ng/L,(329.4 ±56.1)ng/L,(276.5 ±37.1)ng/L,(256.2 ±29.4)ng/L,respectively, which were significantly higher than (109.3 ±16.3 )ng/L in the control group,there were statistically significant differences between the two groups (t＝5.626 10.325,6.885,6.115,all P＜0.05 ).The immediate postoperative VEGF level of the observation group was (329.4 ±56.1)ng/L,which was significantly higher than that before surgery (t＝5.258,P＜0.05 ).The immediate postoperative VEGF level between B group and A group had no statistically significant difference (P＞0.05 ).The serum levels of VEGF in B group at postoperative 2d,7d were (283.2 ± 38.3)ng/L,(261.2 ±21.4)ng/L,respectively,which were significantly higher than those in A group (t＝4.357, 4.529,all P＜0.05).Conclusion The serum VEGF level can sensitively reflect the indicators of coronary ischemia hypoxia and the reconstruction of endothelial injury in patients with AMI,and can be used as the basis for evaluating the effect of revascularization after PCI.