Objective:To investigate the changes in protein metabolism after the use of recombinant human growth hormone(rhGH) in gastrointestinal tumor patients after operation.Methods:Thirty-nine patients with gastrointestinal tumor were randomly(double-blind) divided into two groups: ①the control group(n=20),which was treated with hypocaloric parenteral nutrition and placebo,②the rhGH group(n=19),which was treated with hypocaloric parenteral nutrition and rhGH.The levels IGF-1 and protein metabolism were observed.Results:The concentrations of IGF-1,pre-albumin,transferrin and fibronectin were decreased in the two groups on the 3th day after operation.The administration of rhGH resulted to significant increase in serum levels of IGF-1,pre-albumin,transferrin and fibronectin.Conclusion:The administration of recombinant human growth hormone can increase the protein synthesis and improve the nutritional condition.

ObjectiveTo investigate the expression of a disintegrin-like and metalloproteinase 8 ( ADAM8 ) in breast cancer and in normal breast tissues and its negative regulation role in tumorigenesis and progress of breast cancer.MethodsThe expression of ADAM8 in breast cancer and normal breast tissues was detected by immunohistochemistry (IHC),qRT-PCR,and Western blot.The relation between ADAM8 expression and the clinicopathological parameters of breast cancer patients was analyzed.ResultsADAM8 was expressed in breast cancer and normal breast tissues.The expression of ADAM8 mRNA and protein was significantly lower in breast cancer than in normal breast tissues (qRT-PCR:P =0.015,IHC:P =0.044,Western blot:P =0.000).The expression rate of ADAM8 was related to lymph node metastasis,tumor stage and tumor size,although the difference had no statistical significance.IHC results showed that ADAM8 expression level was lower in stage Ⅲ + Ⅳthan in stage Ⅰ + Ⅱ ( P =0.574 ).qRT-PCR showed ADAM8 mRNA expression was lower in stage Ⅱb + Ⅲ than in stage Ⅰ + Ⅱ a ( P =0.247).ADAM8 expression was lower in the breast cancer tissues with lymph node metastasis than in those without lymph node metastasis (P =0.560 by IHC and P =0.592 by qRT-PCR).ADAM8 expression was lower in tumors whose size ＞ 2 cm than in tumors whose size ≤ 2 cm,however,the difference had no statistical significance ( P ＞ 0.05 ).ConclusionsADAM8 is significantly lower-expressed in breast cancer than in normal breast tissues,which is associated with clinical stages and lymph node metastasis.The reduced expression of ADAM8 may play a role in the pathogenesis and progress of breast cancer.

The medical English level is quite important to the future development ongoing study of medical students,so medical college teachers should take the advantages of Internet and multimedia technology to improve teaching effects.In the present article,we have discussed how to use internet source and multimedia technology to improve the teaching effects of medical English,and pointed out that Internet and multimedia not only enriches the teaching sources and teaching methods,but also creates a better teaching situation and improves the student learning interests,which will bring to good teaching results.

Objective To study the effectiveness of fasudil on the serum high sensitive C-reactive protein (hs-CRP) and serum amyloid A (SAA) in the patients with acute cerebral infarction.Methods 100 patients with acute cerebral infarction were randomly divided into two groups,the control group (n =50 cases) and the treatment group(n =50 cases).The patients in the control group were treated through salvia injection,while the patients in the treatment group were treated through fasudil.They were treated for forteen days.The hs-CRP and SAA in the serum were detected before and after treatment.Results The total efficienties were 92％ in the treatment group and 84％ in the control group.There was a significant difference between two groups(x2 =1.5152,P ＜ 0.05).The treatment group before and after treament with hs-CRP were (11.3 ± 3.7) mg/L,(3.6 ± 1.1) mg/L.The control group before and after treatment with hs-CRP were (10.9 ± 3.5) mg/L,(7.2 ± 2.8) mg/L.Before and after treatment two groups had significant difference (t =14.1053,P ＜ 0.01) ; after the treatment in the two groups had significant diffecence (t =8.4618,P ＜ 0.05).The treatment group before and after treatment with SAA were (17.6 ± 5.2) mg/L,(2.7 ± 0.9)mg/L,control group before and after SAA treatment were (17.1 ± 4.9) mg/L,(5.2 ± 1.8) mg/L.Before and after treatment,two groups had significant difference (t =2.0096,2.0096,all P ＜ 0.01) ; after the treatment in the two groups had significant difference(t =8.7841,P ＜ 0.05).Conclusion Fasudil can improve acute cerebral infarction throughmitigating inflammation.

Objective To evaluate the preoperative diagnosis of ureteral carcinoma.Methods The clinical features and selection of imaging and endoscope in 32 ureteral carcinoma patients were analyzed.Results The common symptom was hematuria.32 cases were confirmed by pathological examination as ureteral carcinoma and the diagnostic corresponding rate was 100% by color doppler and endoscopic technique.Conclusion Imaging and endoscopic examination are very important in hematuria patients without unknown reasons.Ureteral carcinoma should be considered when B mode ultrasound and venous urography show hydronephrosis and no development.Retrograde ureteropyelography and ureteroscopy are the most important diagnostic methods among various examinations.

Objective: To investigate the rural middle school students' mental health and explore its determinants. Methods: 989 rural middle school students in Jiangxi province were investigated with SCL-90, Social Support Rating Scale and the Chinese versions of EMBU. Results: The major problem of mental health were interpersonal sensitivity, obsessive-compulsive, depression, hostility and paranoid ideation. There was remarkable difference between boys and girls, senior and junior high school students. Multiple regression analysis indicated that the major determinants of the rural middle school students' mental health were subjective social support, usage of social support, father's and mother's rejection and denying, father's over-protection, mother's punishment and rigidity, different sex, grades and only children or non-only children. Conclusion: The students in rural middle school, especially in senior high school, had severe mental health problems, social support and parental rearing patterns have a significant influence on mental health.

Objective: To explore the relation between self-esteem and mental health of normal college students.Methods: 386 normal college students finished the Self-esteem Scale(SES),Symptom Checklist 90(SCL-90),Self Consistency and Congruence Scale(SCCS) and College students' Behavioral Inhibition Scale(CBIS).Results: There was close relationship between self-esteem and mental health;those students with high self-esteem had more self consistency and congruence,less symptomatic and behavior inhibition.Conclusion: It indicates that self-esteem can effectively predict mental health for normal college students.

Objective: To observe the changes of free amino acids (FAAs) in plasma and brain of rats during simulated sea sickness and post–adaptation. Method: Pica or kaolin consumption was used as an indicator to judge the development of sea sickness and adaptation when SD rats were stimulated by Crampton sea sickness simulator. FAAs concentrations in plasma and brain of rats were determined by high performance liquid chromatography (HPLC) after simulated sea sickness and post-adaptation. Results: After simulated sea sickness stimulation for 1 d, the levels of Cys and Ile were increased, and Gly and Pro decreased significantly in plasma. The increase of BCAA/AAA ratio was also found. In brain, the contents of Ala, Cys+Met, Tyr, His, and total amino acids were remarkably decreased. After simulated sea sickness stimulation for 21 d, no changes of FAAs were observed in plasma and brain but the ratio of Glu/ GABA was increased in brain. There were no differences of FAAs, Glu/GABA ratio and BCAA/AAA ratio in plasma and brain of tolerant and susceptible sea sickness rats. Conclusion: The levels of amino acids, especially those related to neurotransmitter synthesis, in plasma and brain were changed significantly during sea sickness.

Objective We summarized the clinical experience of modified ileal ureter substitution for treating long segment ureteral defection.Methods We retrospectively analyze the clinical data of 2 patients with long segment ureteral defect who treated with Yang-Monti ileal ureter substitution between March 2015 and November 2015.One 75 years old male patient was diagnosed as upper ureteral malignance and solitary kidney.The length of defection from renal pelvis to bladder was 22 em.His serum creatinine was 100 μmol/L,blood urea nitrogen was 5.7 mmol/L,serum chloride was 98 mmol/L.Another one 41 years old female patient was diagnosed as middle and lower ureteral iatrogenic injury.The traumatic length was 15 cm.Her serum creatinine was 70 μmol/L,blood urea nitrogen was 5.1 mmol/L,serum chloride was 100 mmol/L.they were both treated by Yang-Monti ileal ureter substitution.The ileal intestinal segment was used for the ureteral replacement,which were more than 15 cm to the ilealcecum.The length of intestine was 10.0 cm and 7.5 cm,respectively.The ileal mesentery was preserved.After closing the mesangial hiatus,the ileal segment was pull into the retroperitoneal space and pulling out via descending colonic mesangial window.The ileal segment was divided into three parts,which was 2.5 to 3.0 cm in each part.Each part was opened via long axis and then rotated 90 degree.The 4-0 absorable suture was used to suture the edge of each intestinal part continuously.The sutured intestine was re-tubularized,using 4-0 absorable suture and the F16 catheter was used as the tube model.The length of reconstructed ureter was 22 cm and 18 cm,respectively.The neo-ureter was re-anastomosed with renal pelvis and bladder wall.Two F6 double J stents were placed in the neo-ureter.Results The operative time was 160 min and blood loss was 200 ml in the first case.In the second case,the operative time was 180 min and blood loss was 220 ml.No significant complications were noticed intra-operation and post-operation.Six months after operation,the male patient's serum creatinine was 112 pmol/L,blood urea nitrogen was 6.1 mmol/L,serum chloride was 106.0 mmol/L and electrolytes were normal.In another patient,serum creatinine was 79 μmol/L,blood urea nitrogen was 5.9 mmol/L and serum chloride was 103.0 mmol/L.The GFR was 24.9 ml/min and 22.1 m]/min 3 and 6 months after operation,respectively.Ureteral obstruction wasn't detected on IVU images 3 months after operation.Conclusions For patient with long ureteral defect,which cannot be replaced by other urinary tissue,YangMonti ileal ureter substitution is one of the optional modalities.As a new technique of ureteral substitution,Yang-Monti ileal ureter substitution is simple and fewer complications and can improve the quality of life in patient compared with traditional ureteral substitution.

Objective To evaluate the clinical and pathological characteristics and treatment of small cell carcinoma of the prostate. Methods Two patients with small cell carcinoma of the prostate were reported. Case 1 was 50 year-old. He was admitted with a history of dysuria and perineal pain for 3 months. Digital rectal examination (DRE) showed that the enlarged prostate was 5. 0 cm?6. 0 cm and palpated hard and rough. Low-echo mass was shown on ultrasonography, and heterogeneous density of the prostate on CT. His serum PSA level was 0. 31 ng/ml,and fPSA level was 0.09 ng/ml. Prostate cancer was suspected by biopsy,and radical prostatectomy was performed. Case 2 was 82 year-old. The complaints consisted of dysuria and intermittent gross hematuria for 4 months. The enlarged prostate was 4. 0 cm?5. 0 cm and palpated hard and rough with multiple nodes by DRE. Low-echo mass was shown on ultrasonography, and heterogeneous density of the prostate and involvement of seminal vesicle and bladder neck on CT. His serum PSA level was 2.61 ng/ml,and fPSA level was 0.05 ng/ml. Prostate carcinoma was indicated by biopsy, and orchiectomy plus TURP was performed. Results The diagnosis of small cell carcinoma of the 2 cases were confirmed by postoperative pathology. Microscopically, the tumor cells were arranged in solid-sheet and nest structures, showing the histologic type of diffuse infiltrative carcinoma. Coagulated necrosis could be found easily. Small round or oval cells resembling lymphocytes or oat cells were the main constituents of the tumor. The nuclei were extremely hyperchromatic and scanty. The seminal vesicle and bladder neck had tumor infiltration. The immunohistochemical staining results were negative for LCA,L-26 and 34?E12,but positive for PSA,AE1/ AE3 and AR ,and suspected positive for CgA and S-100. Case 1 died of extensive lung metastasis 1 month after operation. Case 2 had retroperitoneal metastasis of the tumor 3 months after operation, and has been followed till now. Conclusions Small cell cancer of the prostate is rare but can be diagnosed properly based on clinical and pathological features. Radical prostatectomy combined with hormone and chemotherapy is reliable treatment for early stage cancer; but for late stage cancer, there is no effective treatment and the prognosis is poor.