[Objective]To assess the effect of a new invented spondylolisthesis reduction system:elevating-pull reduction system for surgical treatment of lumbar spinal spondylolisthesis associated with isthmic spondylolysis.[Method]Sixty-two patients with lumbar spinal spondylolisthesis were treated with elevating-pull reduction system combined with SRS(spondylolisthesis reduction system),after surgery the patients were evaluated for symptom improvement,intervertebral bone graft fusion,and internal fixation.[Result]The patients were followed up from 1 to 6 years(average 2 years and 5 months) in 62 patients.According to the criterion,excellent results were in 43 patients,good in 16,moderate in 3.No patient showed worse.No internal fixation loosening and breakage were found.[Conclusion]The new invented spondylolisthesis elevating-pull reduction system combined with SRS are more effective and reliable,especially for those osteoporosis patients and serious cases,the reduction and fixation are more effectively.In this procedure the pedicle entrance mouth can be seen straightly and the pedicle screw is inserted more easily.It can prevent the nerve injury,save the operation time,reduce the blood lossing,and excellent outcome can be obtained.

Objective To investigate the treatment of proximal femoral nail antirotation (PFNA) in pertrochanteric hip fractures with unstable lateral-wall.Methods Thirty-two cases were included from the April 2009 to May 2011.Recorded operation period,blood loss volume,and complications and evaluated the effect of the methods.Arrange referral at 6 weeks,3 months,6 months,12 months respectively after operation,to check limb function and conventional camera hips,lateral X-ray film.Postoperative functional evaluation using the Harris hip score.Measure the last follow-up neck shaft angle and compare with postoperative's.Results The average operation period was (60-190) min,average was (80 ± 5) min ; blood loss volume was (90-250)ml,average was (120 ± 9) ml ; Average hospital periods was (15 ± 7) days; weight loading time was (6.3 ± 1.2)weeks after surgery and average time of union of fracture was 3.6 months.All patients obtained union of fracture without complications such as varus,internal fixation cutting out.Conclusion PFNA was proved to be an effective way for treating unstable lateral-wall of pertrochanteric hip fractures due to its advantages of stable fixation,minor trauma,less complications.

Objective To compare the clinical outcomes between proximal femoral nail antirotation (PFNA) combined with risedronate sodium and isolated PFNA for the treatment of intertrochanteric fractures in elderly patients.Methods Clinical data of 62 elderly patients with intertrochanteric fracture from January 2011 to April 2013 were prospectively studied.Patients were randomly divided into two treatment groups:risedronate sodium group (the combined PFNA and risedronate) and control group (isolated PFNA).According to AO classification,32 patients in risedronate sodium group (15 males and 17 females,with an average age of 78 years) were divided into type A1 (n=10),type A2 (n=18) and type A3 (n=4).30 patients in control group (13 males and 17 females,with an average age of 77.5 years) were divided into type A1 (n=9),type A2 (n=16) and type A3 (n=5).Bone mineral density (BMD) was measured by dual energy X-ray absorptiometry before and 1 year after operation.The hip joint function was assessed by Harris hip score.Complications and subsequent fragility fractures were evaluated postoperatively.Results 60 patients (30 patients in risedronate sodium group and 30 patients in control group) were followed up for at least one year.All fractures were healed at 6 months after surgery.No significant difference was found between the two groups in BMD in contra-lateral hip before treatment [(-2.58±0.41)kg/m2 vs.(-2.56±0.36)kg/m2,P＞0.05].BMD in contra-lateral hip had significant difference between the risedronate sodium and control groups one year after surgery [(-0.66±0.37)kg/m2 vs.(-1.13 ±0.28)kg/m2,P=0.000].There was no significant difference between the two groups in Harris hip score (P=0.238).During the follow-up,no patient in risedronate group suffered from subsequent fragility fracture after surgery,while lumbar compression fracture occurred in 1 case,distal radius fracture occurred in 1 case,the contralateral hip fractures occurred in 2 cases,postoperatively.Conclusions PFNA treatment combined with risedronate sodium can effectively improve BMD and reduce the risk for refracture,and has a good effect on intertrochanteric fracture in elderly patients.

[Objective]To observe the clinical therapeutic effect of Jing Kang Yin decoction on nerve-root cervical spondylosis.[Method]152 patients with Cervical Spondylosis neuiodynia were selected and divided into two groups;treatment group with Jing kang Yin decoction(87 cases) and control group with Jingfukang(65 cases).Pain degree(VAS methods),hemorheological indexes,serum cytokine IL-10 and adhesion molecule were observed before and after treatment and the clinical therapeutic effect of two groups were compared.[Result]Pain scores of two groups were lower after treatment than treatment before(P

Objective To investigate the role of activated T lymphocytes in the pathogenesis and effect of CD95 and bcl 2 expression on the development of alopecia areata. Methods The lymphocytes were isolated from the peripheral blood of the patients. Then CD4 +, CD8 +,and/or CD95 and bcl 2 positive lymphocytes were sorted out by flow cytometry . Results In the active stage of the disease, CD4 + lymphocytes decreased while CD8 + lymphocytes increased significantly. At the same time, the expression of bcl 2 was much more frequently detected in CD8 + lymphocytes than in CD4 +lymphocytes. CD95 expression was also increased markedly in T lymphocytes. Conclusions Down regulation of bcl 2 leads to the decrease in number of CD4 + lymphocytes. Meanwhile, CD8 + lymphocytes increased duo to the over expression of bcl 2. As a result , the ratio of CD4 + to CD8 +lymphocytes has dropped markedly and the cytotoxic effect of CD8 +lymphocytes on the hair follicles is enhanced. On the other hand, over expression of CD95 in T lymphocytes triggers cell apoptosis in the hair follicles. Both effects promote the development of alopecia areata . [

The changes of the dietary composition of Shanghai population from 1950 to 1985 were investigated and the relationship between the dietary composition and the mortality of diseases of the population was analysed with retrospective method. The results showed that the amount of consumption of grain was decreased but that of animal foods increased (except that around 1960) year after year during the past 35 years. The consumption of meat and eggs in 1985 was 2.8 and 4.9 times , higher than that in 1950 respectively. The intake of carbohydrates was decreased but- that of fat increased year by year. The ratio of polyunsaturated fat to saturated fats (P/S) was decreased and there was a trend of excess intake of fat. The mortalities from malignant tumours, cardiovascular and cerebrovascular diseases were increased year by year, which correlated strongly with the consumption of dietary saturated fat. On the other hand the intake of protein, calcium and riboflavm was lower. Therefore, the dietary composition of Shanghai population was imperatively adjusted.

Objective To investigate the differences of the working memory tasks' performance between the patients with no neurological syphilis disease and the health controls (HC).Methods 2-back test of N-back task paradigm were applied to measure the performances of working memory.A total of 30 patients with syphilis disease were compared with 35 gender-,age-,years of education-matched healthy persons,then compared the accurate rate and reaction time on the tasks between the two groups.Results Participants with anxiety or depression had to be excluded.The results showed that patients impaired on working memory task.The accurate rate and reaction time of the working memory of the patients was worse than that of the healthy persons ((65.31 ± 8.78) ％ vs (71.00 ±9.64)％ ;(1521.14 ±290.57)ms vs (1296.08 ±253.20)ms respectively) (t=-2.472,P=0.016;t=3.337,P =0.001 respectively).The study indicated the reaction time of the working memory for the patients was negative correlation to the age of final diagnosis (r =-0.362,P =0.049).Conclusion The patients with no neurological syphilis disease show impairment on working memory.This probably because treponema pallidum injures the central nervous system.

Objective To observing the effects of health education on modified lifestyle and behavior patterns among migrant workers. Methods Individuals from two factories were randomly assigned to the intervention group ( n = 262 ; men 129, women 133 ; mean age 31.8) and the control group ( n = 147 ;men 75, women 72; mean age 31.8). The intervention group received 4-months' health education before changes of awareness of health knowledge, cigarette smoking, alcohol consumption, physical activities,mental health and medical consultation. Results In the intervention group, the awareness of the risk of cigarette smoking and alcohol consumption and normal levels of blood pressure and obesity was significantly improved at 4 months(χ2 =59.65, 47.69, 19.50, and 30. 17; all P＜0. 01 ). However, no significant improvement in awareness of AIDS was found in the intervention group. Of the intervention group, some unfavorable lifestyles and behavior patterns were modified ( cigarette smoking χ2 = 4.50 ; altitude toward clinic visit χ2 = 7.09 ; both P＜0. 05 ). Conclusion Health education could help to improve the awareness of health knowledge and modify lifestyle and behavior patterns of migrant workers.

Objective To establish the in vitro biofilm model of Laribacter hongkongensis(LH),to analyze the type Ⅰ integron related genes carried by LH and to investigate the mechanism of LH resistance to quinolones.Methods The biofilm forming abilities of LH clinical isolates were determined by Giemsa staining qualitative method and by crystal violet staining semi-quantitative method.The sensitivity of LH to norfloxacin,ofloxacin,levofloxacin,ciprofloxacin and lomefloxacin in both planktonic and biofilm conditions were dectermined by broth microdilution susceptibility tests.Type I integron related genes carried in 18 LH strains resistant to quinolone were detected by PCR amplification method.Results The detection results by Giemsa staining demonstrated that 36 strains in 55 LH clinical isolates formed visible biofilm,and the biofilm formation rate was 65.4%(36/55).In the biofilm forming ability detected by crystal violet staining semi-quantitative method,OD560≤0.15 was in 8 strains of LH,0.150.20 in 7 strains respectively.The levels of minimal biofilm inhibitory concentration in norfloxacin,ofloxacin,levofloxacin,ciprofloxacin and lomefloxacin to LH were respectively higher than the minimal inhibitory concentration(MIC) in the corresponding floating bacteria(P<0.05).Among 55 strains of LH,18 strains were resistant to quinolones,the resistance rate was 32.7%,and the type I integron in 18 strains of LH carried the drug resistant genes,these drug resistant genes played the drug resistance role in corresponding bacterial strains.Conclusion Drug resistance gene formation and widespread of LH may be associated with type I integron.

Objective To establish an HPLC method to determine the content of diosgenin in Paridis Rhizoma; To examine contents of diosgenin in different varieties of Paridis Rhizoma medicinal plants and from different habitats. Methods Shimadzu Inertsil ODS-C18 guard column (150 mm×4.6 mm, 5μm) was used in HPLC system; elution conditions were the mobile phase using 92% acetonitrile and 8% water (isocraticelution) at a flow rate of 1.0 mL/min with 203 nm detection wavelength. The temperature of the column oven was maintained at 30℃.Results Different varieties of Paridis Rhizoma medicinal plants and Paridis Rhizoma medicinal plants from different habitats contained diosgenin. TheParis polyphylla var.pseudothibetica in Yubei district had the highest content (6.813 7 mg/g);Paris polyphylla var.stenophylla in Chengkou County had the middle content (5.758 4 mg/g);Paris yunnanensisFranch in Shizhu county showed the lowest content (1.952 2 mg/g). The results showed that the obvious difference of chemical quality between various cultivated varieties of Paridis Rhizoma medicinal plants, which might be related with origin, variety and the cultivation technology, and so on.Conclusion The diosgenin contents ofParis polyphylla var. pseudothibetica andParis polyphylla var.stenophylla were higher than that of varieties recorded in Chinese Pharmacopeia.Paris polyphylla var.pseudothibetica andParis polyphylla var.stenophylla had important medical value.