Objective To observe the expression of SGK in decidua in patients with unexplained recurrent spontaneous abortion(URSA) and to investigate its role in the course of apoptosis.Methods The expression of SGK was detected by immunohistochemistry in decidua of URSA(abortion group,n =50) and normal first trimester pregnant women(control group,n =30).The apoptotic index was detected by TUNEL.Results Compared with the control group,the positive expression of SGK decreased significantly in the abortion group,and the difference was statistically significant(x2 =6.78,P ＜ 0.05).The average apoptotic index of the abortion group was (8.19 ± 3.58) ％,which was significantly higher than (2.87 ±1.07)％ of the control group(t =7.94,P＜0.05).Conclusion Intensive apoptosis,the decreasing positive expression rate of SGK in decidua cells may play critical roles in URSA.

AIM: To investigate the dynamic influence of zanthoxylum seed oilA2(ZSOA2) on NF-?B signaling pathway and inflammatory factor in pulmonary tissue of asthmatic mice.METHODS: The suspensoid(0.2 mL containing 20% albumin hydroxide and 10% ovalbumin) was administered by intraperitoneal injection to sensitize the BALB/c mice on day 1,then 0.4% ovalbumin solution(50 ?L in phosphate buffer fluid) was dripped into the respiratory tract through nasal cavity to establish the asthmatic mouse model.After dripped ovalbumin for 24 h,48 h,3 d,7 d and 14 d,the mice were killed at specified time points.The contents of interleukin-4(IL-4),interleukin-5(IL-5) and interferon-?(IFN-?) in bronchoalveolar lavage fluid(BALF) were determined by ELISA.The pathological changes of the lung tissues were observed with HE staining.The inflammatory cell counts were conducted by Eosin staining.The protein levels of adhesion molecule and the molecules of NF-?B signaling pathway in lung tissues were determined by Western blotting.RESULTS: In ZSOA2 treated mice,the pathological injury of the lung was significantly attenuated as compared to the model mice,the counts of eosinophils and lymphocytes were reduced obviously in lung bronchial area of asthmatic mice at all observed time points(P

Objective To analyze mutations in the ATP2A2 gene in a Kazakh family with Darier's disease.Methods Clinical data were collected from 49 members from a family with Darier's disease,and peripheral blood samples were obtained from 44 family members and 100 unrelated healthy people.Genomic DNA was extracted from these blood samples.PCR and DNA sequencing were performed to detect mutations in the ATP2A2 gene.Results Darier's disease was inherited in an autosomal dominant manner in this family.A G→A heterozygous mutation (1288-1G→A) was identified at position 1288-1 at the splice site in exon 12 of the ATP2A2 gene in 11 patients in this family,but not in 33 healthy members or 100 healthy controls.Conclusion Darier's disease in this family may be caused by the heterozygous mutation (1288-1G→A)at the splice site in exon 12 of the ATP2A2 gene.

Objective To establish a quality standard for Jianpibushen granules .Methods The five main ingredients of the formulation :Astragalus membranaceus ,Salvia miltiorrhiza Bunge ,Codonopsis pilosula ,Citrus reticulata Blanco and Paeonia lactif lora Pall ,were identified by thin layer chromatography (TLC) respectively .The content of peoniflorin in Paeo-nia lactif lora was determined by high performance liquid chromatography (HPLC) .The separation was performed on Agilent Eclipse Plus C18 column(4 .6 mm × 250 mm ,5 μm) .The mobile phase was acetonitrile and 0 .1% potassium dihydrogen phos-phate solution for gradient elution .The flow rate was 1 ml/min ,the column temperature was 25 ℃ ,the UV detection was per-formed at 230 nm .Results The spots in TLC were clear without interference in negative control .A good linear relationship was shown within the range of 8 .676-277 .632 μg /ml for peoniflorin (r=0 .999 9) .Conclusion This method is simple ,accu-rate and reproducible ,and can be used as an effective quality control of Jianpibushen granules .

OBJECTIVE: To explore the relationship of exposure to occupational hazards,labor intensity,dietary factors and the metabolic syndrome( MS) in male rolling mill workers. METHODS: A total of 1 105 male workers in a rolling mill were studied as research objects by judgment sampling method. The basic information of the subjects was collected by questionnaire survey and health examination. The prevalence of MS was analyzed according to different individual characteristics of workers. Multi-factor logistic regression analysis was conducted for analysis of the affecting factors of MS.RESULTS: The prevalence of MS in the subjects was 32. 5%( 359/1 105). The prevalence of MS in workers exposed to occupational hazards was higher than that in the control group( 33. 5% vs 20. 2%,P < 0. 05). The prevalence of MS increased with age( P < 0. 05),and decreased with the increase of labor intensity and family per capita monthly income( P < 0. 05). The prevalence of MS in smokers was higher than that in non-smokers( P < 0. 017),and the prevalence of MS in workers with animal dietary was higher than those with balanced dietary and with traditional dietary( P < 0. 017).The multivariate logistic regression analysis showed that after the adjustment of confounding factors such as age,seniority,family per capita monthly income,smoking and drinking,MS risk in a single factor of carbon monoxide exposure group,high temperature combined with carbon monoxide exposure group and high temperature combined with noise exposure group were higher than that of the control group without exposure to occupational hazards respectively( P < 0. 05),and the ratio of the 95% confidence interval were 2. 246( 1. 097-4. 597),2. 581( 1. 308-5. 094) and 2. 435( 1. 281-4. 627). The risk of MS in workers with animal dietary was higher than that with traditional dietary( P < 0. 01),the odds ratio and 95%confidence interval was 1. 497( 1. 106-2. 025). The grade of MS was 24. 6% lower than the original labor intensity with each increasing grade of risk( P < 0. 05). CONCLUSION: The animal dietary,increased labor intensity,exposure to carbon monoxide,high temperature combined with carbon monoxide and high temperature combined with noise can all increase the risk of MS.

Objective: To investigate the value of means of surfaces with nonhomogeneity (MSN) in esti-mating the incidence of pneumoconiosis. Methods: Based on the principal component analysis, all counties (districts) of Hebei Province, China, were divided into three categories according to the degree of pneumoconiosis hazards and the MSN model was used to estimate the incidence rate of pneumoconiosis and the number of pneu-moconiosis cases using the data of the incidence of pneumoconiosis in 2010. Results: With reference to the appli-cation requirements of the MSN model, results of the principal component analysis, and expert experience, the 172 counties (districts) in Hebei Province were divided into three categories with mild, moderate, and severe pneumoconiosis hazards. There were 74, 61, and 49 counties in the above categories, respectively, and 12, 12, and 25 counties were selected from them, respectively. The estimated number of pneumoconiosis cases in Hebei Province was 2105, and the incidence rate was 261.5 per hundred thousand, with a standard error of esti-mation of 389.9 per hundred thousand. Conclusion The MSN model provides a new thought and method for es-timating the number of pneumoconiosis cases.

Objective To detect the level of 5-hydroxymethyl-cytosine (5-hmc)in melanoma tissues,and to analyze the correlation between 5-hmc and the invasion,metastasis and prognosis of melanoma.Methods A streptavidin-peroxidase immunohistochemical method was used to detect the level of 5-hmc in 67 melanoma tissues and 20 pigmented nevi tissues.Univariate and multivariate analyses were performed with the Cox's proportional hazards regression model to analyze the correlation between the expression of 5-hmc and the prognosis of melanoma.Results The expression rate of 5-hmc was significantly lower in melanoma tissues than in pigmented nevus tissues (40.30％ [27/67] vs.75％ [15/20],22 =7.428,P =0.006).According to American Joint Committee on Cancer (AJCC) TNM staging system,the expression level of 5-hmc was significantly lower in the stage Ⅳ melanoma tissues than in the stage Ⅱ and stage Ⅲ melanoma tissues (x2 =4.416,P =0.036).Patients with lymph node metastasis showed significantly lower expression of 5-hmc compared with those without lymph node metastasis (x2 =5.902,P =0.015),and the level of 5-hmc expression significantly decreased along with the increase of Clark grade (x2 =4.828,P =0.028).There were no significant differences in the level of 5-hmc expression between patients of different ages,genders or nationalities (P ＞ 0.05).Multivariate Cox regression analysis showed that distant lymph node metastasis (HR:2.67,95％ CI:1.22-5.84),not receiving surgical resection (HR:0.41,95％ CI:0.18-0.95),and low expression of 5-hmc (HR:3.54,95％ CI:1.09-11.43)were independent risk factors for poor prognosis of melanoma.Conclusion 5-Hmc may participate in the invasion and metastasis of melanoma,and be associated with the prognosis of melanoma.

The management of drug clinical trial institutions from the risk management point of view is investigated. According to the technology of risk assessment in risk management, taking the project audit as an example, basing on the basic procedures of risk identification, risk assessment and risk control, making 8 risk factors into the table of risk management for project approval of clinical trial in order to initially establish a risk management of drug clinical trial institutions. In this way, the management quality of project for drug clinical trials will be probably improved, so that the risk incidence rate will be effectively reduced in the later phase of clinical trial.

Objective:To understand the overall development and dynamic change trend of scientific research input-output efficiency of a tertiary hospital in Xinjiang from 2016 to 2021, so as to provide reference and basis for optimizing hospital scientific research configuration and improving scientific research efficiency.Methods:The BCC model of Data Envelopment Analysis (DEA) method is combined with Malmquist index method for analysis and evaluation.Results:Within 6 years, the average value of comprehensive technical efficiency of nursing units has not reached 1.The total factor productivity of nursing units was unstable, showing an alternating rise and fall trend, with a fluctuation range of 0.749~1.140.Conclusions:The overall efficiency of scientific research in clinical departments of hospitals is not high. Hospitals should implement multi-level scientific research evaluation, form a dynamic evaluation system for scientific research, reasonably optimize and supervise the investment and utilization of scientific research resources, improve the scientific research management system, enhance the scientific research awareness of researchers, promote technological progress of researchers, and thereby improve scientific research efficiency.

Objective:To understand the cognition of research integrity of in-service medical staff in a tertiary hospital in Xinjiang, provide possible reference for research integrity training.Methods:In March 2021, an electronic questionnaire survey was conducted to investigate the knowledge of scientific research integrity of in-service medical staff in a tertiary hospital in Xinjiang. T test and analysis of variance were used for univariate analysis, and multiple linear regression was used for multivariate analysis.Results:550 valid questionnaires were collected in total, and the overall awareness rate of scientific research integrity of in-service medical staff was less than 60%. Univariate analysis showed that there were differences in cognitive score of scientific research integrity among medical staff who participating in different scientific research activities ( P<0.05). Multivariate analysis showed that the educational level of the in-service medical staff, whether they participated in scientific research training, whether they had the qualifications of graduate student supervisor, the interaction between educational level and participation in scientific research training was statistically significant ( P<0.05). LSD test of educational level and participation in scientific research training showed that there was a statistically significant difference in educational level of participation in scientific research training ( P<0.01). Conclusions:The cognition of research integrity on in-service medical staff in a tertiary hospital in Xinjiang need to be enhanced, and their knowledge of research integrity need to be improved. Various measures should be carried out to strengthen the research integrity education and training, so as to improve the level of medical staff’s cognition of research integrity, and improve the medical staff’s attitudes of research integrity.