Objective To explore the distributions of C AG polymorphisms of androgen receptor gene in Chinese population and the relatio nship with prostate cancer (CaP). Methods The number of CAG repeats were measured in 105 cases with CaP and 190 matched case-control b y PCR、DHPLC and Sequencing analysis,and its association with the susceptibility to CaP. Results The average number of CAG repeats was 2 2.7 in CaP and 23.3 in control group ( P =0.22).Men with CAG repeats lengt h less than 22 may be in high risk of prostate cancer (odds ratio,2.39;95%CI 1.1 9～4.81, P =0.012),compared with men CAG repeats length of 22 or more. Conclusions A less CAG repeat length (

Objective To investigate the effect of radix salviae milliorhize on hemodynamics of portal hypertensive rats.Methods 60 Wistar rat models with portal hypertension were induced by CCl_4.Among them 56 rats with portal hypertension were divided at random into 4 groups: radix salviae 3 d group,radix salviae 10 d group,radix salviae 15 d group,and control group.After administration,the blood flow of vena portae,superior mesenteric artery,superior mesenteric artery and pressure of vena portae,average arterial pressure were measured.(Results Compared) with 0.9% Natrii Chloridi control group,the blood flow of vena portae,superior mesenteric artery,superior mesenteric artery and pressure of vena portae,average artery pressure in radix salviae milliorrhize 3,10 and 15 d groups were decreased significantly(P0.05).Conclusion Radix salviae milliorhize can be employed for treating portal hypertension.Radix salviae milliorrhize can decrease the blood flow and portal pressure,moreover,the effect can be enhanced with the elongation of treatment time.

OBJECTIVE To study the usage of antibiotics in aseptic operation,and strengthen the management of antibiotics usage. METHODS We investgated the usage of antibiotics in aseptic operation retrospectively,then statistically analyzed. RESULTS Among all the 347 cases,343 cases were used for prophylaxis,the usage rate was 98.85%.The mean time of antibiotic therapy was 7.22 days.90.96% of the patients were treated with antibiotics for 3 days or more,53.35% of the patients were treated beyond 7 days.Ninety one patients were given antibiotics 0.5-2 hours before the operation(26.53%).There were 7 kinds of drugs used in the operation,the cephalosporin class accounted for 70.26%,moreover was third generation cephalosporin. CONCLUSIONS The prophylactic application of antibiotics in the aseptic surgery has some unreasonable phenomenon and must be strengthened.Training the doctor to use the antibiotics reasonably,and the effective surveillance management mechanism be established.

The circulating antigens of Schistosoma japonicum and the antibodies against the worm in the sera of patients were detected concurrently by Dot-ELISA.In 30 sera from patients with acute schistosomiasis, 51 sera from patients with chronic schistosomiasis and 30 sera from patients being treated by praziquantel 5=6 years ago, the positive rates of the circulating antigens were 86.7%, 78.4% and 23.3% respectively, but all negative in 30 sera from patients with clonorchiasis sinensis. One of the 40 sera from healthy individuals had a weak positive reaction. The positive rates of the antibodies in those acute, chronic and treated patients with schistosomiasis were 100.0%,96.1% and 30.0%; the mean titers of antibodies were 1 : 367.6, 1 : 181.7 and 1 : 9.8 respectively; Two sera from patients with clonorchiasis were positive. All of the healthy individuals were negative.The results indicaded that the circulating antigen was in accord with the antibodies in most sera from schistosomiasis patients.

Objective To learn the epidemiological characteristics of syphilis in Shihezi in recent three years and to provide bases for prevention .Epidemiological analysis with syphilis was conducted in our hospital from 2012 to 2014 ,which is about the detection rate ,age ,gender ,and the distribution in the department .Methods Serum were deteceted by using three methods and the data were analyzed .Results 1 281 syphilis cases in 74 798 patients were detected in our hospital during this period .The total positve rate of three years was 1 .71% .The positve rate was 1 .35% ,1 .83% and 2 .01% respectively .The results of three years was significant differences(χ2 =39 .877 ,P<0 .05) .The rate in gender was not significantly higher or lower(χ2 =1 .670 ,P=0 .434>0 .05) .The ca‐ses were mainly distributed in Han(581 ,45 .36% ) ,Uygur(43 ,3 .36% ) ,Kazak(23 ,1 .80% ) .353 cases were negative by RPR among 1 281 patients with syphilis(27 .56% ) ,and the negative rises year by year .Conclusion The incidence of syphilis increased slightly in Shihezi since 2012 .Though the detection rate between 2013 and 2014 was no significant difference .The focus was on 20- year′s old women of childbearing age .It is necessary to take strict measures to control the spread of syphilis and to do syphilis examina‐tion for all inpatients in order to prevent the infection of syphilis .

Objective To assess the effects of medicated permeation with traditional Chinese medicine Shujin-Zhitong liquid combined with ion tophoresis on osteoarthritis(OA) of rabbit knee joint in vivo.Methods The modified Hulth OA model was prepared with rabbits by anterior cruciate ligament desmotomy plus medial meniscus resection.Twenty-four model rabbits were randomly divided into three groups: Chinese medicine group: treated with Chinese medicine Shujin-Zhitong liquid by ion tophoresis for 20 minutes per day;positive control group: glucosamine hydrochloride was administered orally per day;blank control group: no treatment was administered to the model rabbits.Serum nitrogen monoxidum(NO) concentration was determined at the time points of prior model making,prior treatment,and 4 and 8 weeks of treatment.At 4 and 8 weeks of treatment,the apoptotic rate of chondrocytes was determined by flow cytometry,and the gene expressions of collagen Ⅱand aggrecan were determined by reverse transcription polymerase chain reaction(RT-PCR).Results The contents of serum NO in Chinese medicine group and positive control group were lower than that in blank control group at 4 weeks after treatment,and the content of serum NO in Chinese medicine group was lower than those in positive control group and blank control group at 8 weeks after treatment.The apoptotic rates in Chinese medicine group and positive control group were lower than that in blank control group at 4 and 8 weeks after treatment.Gene expression levels of collagen Ⅱ and aggrecan in Chinese medicine group and positive control group were higher than that in blank control group at 4 weeks after treatment,while the gene expression level in Chinese medicine group was higher than those in positive and blank control groups at 8 weeks after treatment.Conclusions The treatment of medicated permeation with traditional Chinese medicine Shujin-Zhitong liquid combined with ion tophoresis has shown good therapeutic effect on osteoarthritis(OA) of rabbit knee joint,the mechanism might be the inhibition of chondrocytes apoptosis and reduced secretion of inflammatory factors,and the improvement of cartilage repair by up-regulating related gene expression of chondrocyte.

Objective To detect the transfusion transmitted virus-like mini virus(TLMV) sequence gene in patients with chronic hepatitis and to study the TLMV infection in population.Methods Two sets of primers in the most conservative regions of Japan strains TLMV-CBD279 and CBD231 were designed to amplify TLMV templete extracted from sera of patients with chronic hepatitis B using PCR.PCR products were cloned into pGEMR-T vector and sequenced.Results The results showed that 72%of TLMV sequence isolated in China was identical to that in Japan,suggesting that there was TLMV infection in patients with chronic hepatitis B in China.The patients with hepatitis virus C and the healthy blood donor had the highest infectious rates of TLMV.Conclusion TLMV infection exists in patients with chronic hepatitis B in China.

OBJECTIVE To investigate the microorganisms on the hands of nurses who are doing the intravenous infusion.METHODS Sampled the hands of nurses during the procedure of intravenous infusion randomly,and then sampled after monitoring the nurses washing their hands by the six steps hand-cleaning technique.Then cultured the samples and did the identification of bacteria.The distribution of microorganism on nurses' hands was analyzed.RESULTS During the procedure of intravenous infusion,before and after the intervention the microorganisms on the nurses' hands had evident difference and with the statistic meanings.CONCLUSIONS The handwashing compliance among nurses before working in our hospital is poor,and the hand hygiene status during the nursing procedure needs to be improved.

Objective To investigate the changes of Th1/Th2 cytokines levels in serum in patients with chronic hepatitis B patients befroe and after treatment of thymosin,and clarify the relationship with state of illness,curative effect and virological response.Methods 19 patients with chronic active hepatitis B were chosen as treatment group,10 cases as normal control group.The patients in treatment group were treated with injection of thymosin ? 1 mL,in the pre-treatment,13 and 21 weeks after treatment the cytokine levels,HBV DNA,and biochemical indicators of liver function were detected by flow cytomertry on Tuesday,Friday every week;in normal control group the serum IL-2,IFN,TNF,IL-4,IL-6,IL-10 indicators were determined and the relationship between cytokine levels and biochemical responses and virological response was analyzed.Results After thymosin treatment,the cytokine levels secreted by Th cells were increased compared with pre-treatment (P

Objective To evaluate the influence of atopy on exhaled nitric oxide in chronic persistent asthmatic children. Methods A total of 52 chronic persistent asthmatic children who completed FeNO measurements and skin prick testing were enrolled. Patients were divided into non-atopic group and atopic group by skin prick testing results, and subdivided into non-allergic rhinitis and rhinitis group according to whether combined with allergic rhinitis. At the same time 78 healthy children were chosen as control group. Moreover, 32 chronic persistent asthmatic children who completed FeNO measurements twice interval of three months were enrolled. Results The FeNO level was signiifcantly different among the atopic group (n=40), the non-atopic group (n=12) and the control group (H=33.29, P=0.000);The FeNO level was signiifcantly higher in the atopic group than that in the non-atopic group (P<0.05). And the FeNO level were signiifcantly different among the rhinitis group (n=41), the non-rhinitis group (n=11) and the control group (H=30.63, P=0.000). The FeNO level was signiifcantly higher in the rhinitis group than that in the control group (P<0.05), however there were no difference between the rhinitis group and the non-rhinitis group(P>0.05).There were no correlations between FeNO levels of chronic persistent asthmatic children and the wheal diameter of house dust mites or dust mites (r=2.05, P=0.135;r=1.58, P=0.312). Moreover, the FeNO level was signiifcantly lower after 3 months ICS treatment (z=-2.05, P=0.041). Conclusions Atopy had major inlfuence on the FeNO level of chronic persistent asthmatic children, and the FeNO level declined with the theatment of ICS.