1.Progresses on mechanism studies for cell apoptosis
Junqi FENG ; Xiulan LI ; Renxiao BAI
International Journal of Biomedical Engineering 2006;0(01):-
Cell apoptosis is an important biological process. It plays an essential role on cell growth and response for outside stimulations. This review focused on the recent advancement of apoptotic signal transduction, enzymological mechanisms, the function of mitochondria in cell apoptosis as well as the regulation of genes.
2.Quality Evaluation of the Decoctions of Platycladus cacumen Between the Precise Powder Decoction Pieces and Traditional Chinese Medical (TCM) Slices
Lu GONG ; Junqi BAI ; He SU ; Peng ZHANG ; Shuiming XIAO ; Xiwen LI ; Zhihai HUANG ; Jiang XU
World Science and Technology-Modernization of Traditional Chinese Medicine 2017;19(1):108-113
This study aimed at comparing the precise powder decoction pieces and market raw TCM slices of P.cacumen over the decocting quality.ITS2 sequence was adopted as a DNA barcode to identify P.cacumen.The chemical composition of the medicinal materials was characterized by HPLC fingerprints for the evaluation of the similarity of precise powder decoction pieces and market TCM slices.The concentrations of quercitrin were determined using UPLC,and the characteristic common peaks were identified.In addition,the extraction efficiency between the market TCM slices and the precise powder decoction pieces was also compared by standard decoction method.It was found that P.cacumen was accurately identified by ITS2 sequences.HPLC fingerprints showed that the extraction efficiency and similarity of the precise powder decoction pieces increased compared with the market TCM slices.However,the extraction yield rate of the precise powder decoction pieces was improved by 20% increased in accordance with the standard decoction method,while the contents of the index component,quercitrin,presented rare increase and the decocting rates of the other chemical components little change in the study.In conclusion,it was indicated that precise powder decoction pieces improved the extraction efficiency and uniformity in comparison with TCM slices.
3.Comparison of Precise Powder Decoction Pieces and Original Traditional Chinese Medical Slices of Rhizomatic—A Case Study on Fallopia multiflora Radix Preparata
Junqi BAI ; Zhihai HUANG ; Juan HUANG ; Lu GONG ; Shuiming XIAO ; Xiwen LI ; Jiang XU ; Xiaohui QIU
World Science and Technology-Modernization of Traditional Chinese Medicine 2017;19(1):95-100
This study aimed at investigating the drug preparation of precise powder decoction pieces (PPDP) system,Fallopia multiflora radix preparata (FMRP) was employed in this study.Different specifications of PPDP were prepared,their extract rates were in contrast with the original pieces.Compared the quality uniformity of three batches between FMRP original slices and its PPDP extraction,the similarity of the chemical fingerprints was evaluated,and the contents of common peaks and quality uniformity were compared by relative peak areas.ITS2 sequence was taken as a DNA barcode to identify F.multiflora radix (FMR).As a result,the extract rate of PPDP was 2.5 times as much as the original slices.The average content of stilbene glucoside from the three original slices and the PPDP extraction were 3.56 ± 2.61 and 13.23 ± 0.37 mg·g-1,respectively;while the RSD were 73.28% and 2.82%.The similarity of the fingerprints of the PPDP extraction was almost the same as that of the original slices,but the content and the uniformity of the common peaks of the PPDP extraction were significantly improved.Thus,FMR was accurately identified using ITS2 sequences.It was concluded that the PPDP considerably improve the decocting rate and quality uniformity,indicating that PPDP could save resources and improve the clinical efficacy.
4.Effect of emodin on rat bone marrow mesenchymal stem cell proliferation and mRNA expressions of hematopoietic growth factors.
Mingchao LIU ; Jianan WEI ; Junqi BAI ; Lan GUO ; Lingling OUYANG ; Xiaohui QIU
Journal of Southern Medical University 2014;34(5):736-739
OBJECTIVETo study the effect of emodin on the proliferation, cell cycle distribution, apoptosis and expression of hematopoietic growth factors in bone marrow mesenchymal stem cells (BMSCs).
METHODSThe proliferation of rat BMSCs exposed to emodin was analyzed using MTT assay, and flow cytometry was used to detect the apoptosis and cell cycle changes of the exposed cells. Real-time quantitative PCR was used to determine the mRNA expression of the hematopoietic growth factors.
RESULTSExposure to 0.1 and 1 µg/ml emodin for 48 and 72 h significantly enhanced the proliferation of BMSCs (P<0.01). The cells exposed to 0.1 µg/ml emodin showed significantly increased percentage of cells in G2/M phase (P<0.05), and 1 µg/ml emodin exposure caused increased cells in S phase (P<0.01) and decreased cells in G1/G0 phase (P<0.05). Emodin exposure for 48 h resulted in significantly decreased cell apoptosis (P<0.05). BMSCs treated with 0.1 µg/ml emodin showed a significant increase in the expression of thrombopoietin mRNA (P<0.05).
CONCLUSIONEmodin can promote the proliferation of BMSCs in vitro possibly by regulating the cell cycle distribution, cell apoptosis and thrombopoietin expression.
Animals ; Apoptosis ; Cell Cycle ; Cell Proliferation ; drug effects ; Emodin ; pharmacology ; Hematopoietic Cell Growth Factors ; metabolism ; Mesenchymal Stromal Cells ; cytology ; drug effects ; RNA, Messenger ; Rats
5.Research progress in the synthesis of tumor-associated carbohydrate antigens (TACAs) GM3 and de rivatives
Guojing BAI ; Xiaojie BIAN ; Kuo YAO ; Junqi WU ; Shichong YU ; Qingguo MENG ; Qiuye WU
Journal of Pharmaceutical Practice 2016;(1):5-7,15
Glycosylation is the key step of the synthesis of GM3 ,its reaction conditions are very harsh ,the stereoselec‐tivities are usually poor ,and the configuration of anomeric carbon is difficult to control .Whetherαglycosidic bond can be con‐structed efficiently in sialylation reactions is an important criteria used to evaluate the reaction quality .Studies of GM3 and de‐rivatives methods generally relates to following areas :the choice of the donor compounds and receptor compounds ,the control of stereoselectivity ,and the development of some new glycosidic reaction catalyst .In recent years ,important progress has been made in this research area .Now ,we predominately make a summary and review on the progress of methods for the synthesis of GM3 and derivatives .
6.Feasibility, reliability and validity of a 3D scanning volume measurement for distal upper limb volume
Xiao QIU ; Junqi LING ; Yangyang CONG ; Yulong BAI
Chinese Journal of Rehabilitation Theory and Practice 2023;29(3):364-367
ObjectiveTo clarify the feasibility of the 3D scanning volume method for distal upper limb volume measurement, and to analyze its scorer reliability and criterion-related validity. MethodsFrom January to March, 2022, a therapist (operator A) who had not been exposed to 3D scanning volume method and water displacement method was trained to use a handheld 3D laser scanner and a spilt cup to measure the volume of a PVC distal upper limb model. The operation time of 30 operations of each method was recorded. The learning curves of the two methods were plotted using cumulative sum (CUSUM) analysis. The curve was cut into the learning stage and the mastery stage by the vertex of peak. The times required to reach the mastery stage and the operation time of the mastery stage for the two methods were recorded. A total of 20 healthy subjects were recruited from Huashan Hospital of Fudan University. Two trained therapists (operator A and operator B) measured the bilateral distal upper limb volume using a handheld 3D laser scanner, and operator A measured the bilateral distal upper limb volume using a spilt cup. ResultsThe fitting learning curve of the 3D scanning volume method (R² = 0.984) reached its peak after eight times of operation; while that of the water displacement method (R² = 0.494) reached its peak after five times of operation. At mastery stage, the operator spent less time using 3D scanning volume method than using water displacement method (P < 0.05). The intraclass correlation coefficient between the two operators were both 0.979 for bilateral distal upper limb volume measure (P < 0.001). The Pearson coefficients was above 0.979 between 3D scanning volume method and water displacement method (P < 0.001). ConclusionA therapist can master the use of the 3D scanning volume method after eight times of operation, and the operation time of 3D scanning volume method is shorter than that of water displacement method at mastery stage. The 3D scanning volume method is well reliable and valid, that can be used as an alternative to the water displacement method for distal upper limb volumetric measurement.
7.Effect of wrist-hand orthosis combined with modified constraint-induced movement therapy on upper limb and hand function in patients with stroke
Songhua HUANG ; Junqi LING ; Tianhao GAO ; Yijia HUANG ; Yulong BAI
Chinese Journal of Rehabilitation Theory and Practice 2024;30(5):606-612
ObjectiveTo observe the effect of wrist-hand orthosis combined with modified constraint-induced movement therapy (mCIMT) on upper limb and hand function in patients with stroke. MethodsFrom February, 2022 to December, 2023, 32 patients after stroke in Huashan Hospital, Fudan University were randomly assigned to control group (n = 16) and experimental group (n = 16). Both groups underwent routine rehabilitation, and wore constraint glove almost four hours a day. The experimenal group wore dynamic wrist-hand orthosis four hours everyday, additionally; five days every week, for three weeks. They were evaluated with Wolf Motor Function Test (WMFT), Action Reach Arm Test (ARAT), the strength of gripping, Amount of Use (AOU) and Quality of Movement (QOM) of Motor Activity Log (MAL), Hamilton Anxiety Scale (HAMA) and Hamilton Depression Scale (HAMD) before and after treatment, while root mean square ratio of affected/healthy extensor muscle of wrist was measured with surface electromyography ResultsAfter treatment, the scores of WMFT, ARAT, MAL-QOM, HAMA and the root mean square ratio of affected/healthy extensor muscle of wrist improved in both groups (|t| > 2.179, P < 0.05), and the improvement of WMFT score and the strength of gripping was greater in the experimental group than in the control group (|t| > 2.343, P < 0.05); the strength of gripping, the scores of MAL-AOU and HAMD improved in the experimental group (|t| > 2.819, P < 0.05). ConclusionmCIMT assisted with dynamic wrist-hand orthosis could improve upper limb and hand function in stroke patients.
8.Analysis of Metabolites and Metabolism Pathway of Tetrahydroxystilbene Glucoside in Rats with UHPLC-MS and Mass Defect Filter Method
Youling LIANG ; Xuhua SHI ; Junqi BAI ; Zhihai HUANG ; Wen XU ; Juan HUANG ; Xiaohui QIU
China Pharmacy 2021;32(6):675-681
OBJECTIVE:To analyze the metabolites of tetrahydroxystilbene glucoside (THSG)and speculate its metabolism pathway in rats. METHODS :Male SD rats were randomly divided into plasma group (n=3),urine group (n=3),bile group (n=3),and tissue group (n=9). Each group was given single dose of THSG 200 mg/kg intragastrically. Plasma samples 10,30 min and 1,1.5,2,4 h after medication ,the unrine 0-6 h after medication ,the bile 0-4 h after medication ,the tissue of heart , liver,spleen,lung,kidney and stomach 30 min and 1,2 h after medication (3 at each time point )were collected respectively.After precipitated with methanol ,the metabolites of samples were analyzed and identified by UHPLC-Q-Exactive Orbitrap MS and mass loss filtration (MDF). Its metabolism pathway was speculated. RESULTS:In the blood ,urine,bile,heart,liver,spleen, lung,kidney,stomach samples ,6,7,11,1,5,1,3,4,4 metabolites were detected ,including two phase Ⅰ(hydrolysis, hydrogenation and hydroxylation )metabolites,18 phase Ⅱ(glucuronic acid binding and sulfation )metabolites. There were 12 glucuronic acid binding products. CONCLUSIONS:Most of the metabolites of THSG are found in bile ,mainly glucuronic acid binding products of phase Ⅱ metabolite THSG ; main metabolic pathways involve glucose hydrolysis , hydrogenation, hydroxylation,glucuronic acid binding and sulfation.
9.Establishment of HPLC Fingerprint and Content Determination of 5 Kinds of Isoflavone Components in Sojae Semen Nigrum
Qianxiang GUO ; Youling LIANG ; Xuhua SHI ; Junqi BAI ; Juan HUANG ; Zhihai HUANG ; Xiaohui QIU
China Pharmacy 2020;31(4):428-434
OBJECTIVE:To establ ish the fingerprint of Sojae Semen Nigrum and content determination method of 5 kinds of isoflavones,so as to provide reference for controlling its quality better. METHODS :HPLC method was adopted to establish the fingerprint and detect the contents of 5 kinds of isoflavones. The determination was performed on Phenomenex C 18 column with mobile phase consisted of acetonitrile- 0.12% formic acid solution (gradient elution )at the flow rate of 1 mL/min. The detection wavelength was set at 260 nm;the column temperature was 30 ℃ and sample size was 10 μL. Using daidzin as reference,HPLC fingerprints of 12 batches of samples were determined. The similarity of 12 batches of samples was evaluated by TCM Chromatographic Fingerprint Similarity Evaluation System (2012A) to confirm common peak. Cluster analysis and principal component analysis were performed by using SPSS 20.0 software and SIMCA 13.0 software. RESULTS :There were 19 common peaks in HPLC fingerprints of 12 batches of samples ,the similarity of which was higher than 0.94. Totally 5 components were identified,such as daidzin ,glycitin,genistin,daidzein,genistein. Cluster analysis showed that 12 batches of Sojae Semen Nigrum were clustered into 2 categories,i.e. S 1-S3 clustered into one category ,and S 4-S12 clustered into the other category. By principal component analysis ,the contribution rates of two principle components were 53.261% and 40.715%;accumulative contribution rate was 93.976%. The linear range of above 5 components were 5.97-191.00 µg/mL(r=0.999 9),1.05-33.46 µg/mL(r=0.999 9), 8.93-285.61 µg/mL(r=0.999 5),0.82-26.33 µg/mL(r=0.999 9),0.93-29.64 µg/mL(r=0.999 7),respectively. The limits of quantitation were 0.881 1,0.611 6,0.078 6,0.243 3,0.511 6 μg/mL,respectively. The limits of detection were 0.264 3,0.244 7, 0.021 4,0.124 8,0.106 7 μg/mL,respectively. RSDs of precision ,stability,reproducibility and durability tests were all lower than 5%. Recoveries were 95.15%-96.56%(RSD=0.51%,n=6),98.52%-103.45%(RSD=1.88%,n=6),95.37%-97.91% (RSD=0.95%,n=6),99.75%-102.00%(RSD=0.78%,n=6),100.26%-103.65%(RSD=1.21%,n=6). Among 12 batches of Sojae Semen Nigrum ,the contents of above 5 components were 0.178 3-0.265 9,0.021 7-0.096 2,0.288 5-0.597 2,0.014 1- 0.058 8,0.012 9-0.082 9 mg/g. CONCLUSIONS :Established HPLC fingerprint and content determination method of 5 kinds of isoflavones can be used for quality control of Sojea Semen Nigrum. The Isoflavone components are similar ,but the contents are different among Sojae Semen Nigrum from different producing areas.