1.Construction of GFP reconbinant AAV- reporter virus and its expression in NIH 3T3 cell
Journal of Xi'an Jiaotong University(Medical Sciences) 1982;0(04):-
Objective To construct a recombinant AAV reporter virus expressing GFP and observe its expression in NIH 3T3 cell. Methods The resulting gene of GFP was subcloned into the site EcoRⅠand BamHⅠof vector pssHGCMV, and a recombinant adeno-associated virus (rAAV) vector pssHGCMV-GFP expressing GFP was constructed by using DNA recombinant technique. The 143 cells were transfected with the vector and the rAAV stock expressing GFP was packaged, produced and purified. The titre of the rAAV stock was measured by quantitative dot blot hybridization. NIH 3T3 cells were infected with the rAAV and the fluorescence was detected in alive cells to confirm successful infection and the expression of exogenous gene in NIH 3T3 cell line. Results A GFP-expressing rAAV vector VssCMV-GFP was successfully constructed. The titer of the VssCMV-GFP was 3.16?10~(12) particles/mL. GFP was initially expressed in the NIH 3T3 cells 48h following infected by VssCMV-GFP and subsequently it gradually increased. The green fluorescence in the cells was the most apparent 120h after infected. Conclusion VssCMV-GFP can infect the eukaryotic cells and GFP can be expressed in the alive cells.It is suggested that rAAV-mediated transfer of the gene to the eukaryotic cells can serve as a delivery system of gene therapy.
2.Expression of ICAM-1 and VCAM-1 in human chronic renal allograft rejection
Xiaoming PAN ; Yong CHEN ; Junping XING
Chinese Journal of Organ Transplantation 1998;19(4):217-219
To study the mechanism of human chronic renal allograft rejection, kidney tissues were taken from 16 patients with chronic renal allograft rejection and from 5 healthy subjects, and underwent the frazed section staining for ICAM-1 and VCAM-1 to anti-ICAM-1 and anti-VCAM-1 respectively by using immunohistochemistry(ABC).The results showed that there were differ-ent distribution of ICAM-1 and VCAM-1 expression in nomal kidney and renal allograft during chronic rejection.It was suggested that ICAM-1 and VCAM-1 might play an important role in the pathogenesis of human chronic renal allograft rejection.
3.Progress in intestinal permeability related diseases and its influencing factors
Panpan XING ; Ping LIU ; Junping WANG
Chinese Journal of General Practitioners 2017;16(4):320-323
The intestinal permeability is crucial for human health,and the changes of intestinal permeability may cause variety of diseases,which is widely concerned.This article reviews the latest research progress in the relationship between intestinal permeability and diseases,the main influencing factors of intestinal permeability and its common testing methods,which may provide new ideas for the clinical treatment of related diseases.
4.Relationship between intercellular adhesion molecule-1 and HLA-DR expression in the renal allograft of chronic rejection
Junping XING ; Hua ZHOU ; Xiaotong WU
Chinese Journal of Urology 2001;0(09):-
Objective To study the relationship between the expression of ICAM-1,HLA-DR in the renal allograft of chronic rejection. Methods The expression of ICAM-1 and HLA-DR was assessed in 20 cadaveric renal allografts with chronic rejection using immunohistological techniqu(ABC method). Results In the renal allograft with chronic rejection,the expression of ICAM-1 was increased on the tubular epithelial cells and interstitial microvascullar endothelium,whereas the expression of HLA-DR was up-regulated,especially on the distal tubules. In addition, the expression of ICAM-1 and HLA-DR was associated with lymphcytes infiltration in the local perivascullar and intertubular structure. Conclusions It is suggested that the increased ICAM-1 and HLA-DR expressions might mediate allograft injury and have a role in the augmentation of the response,either in its induction,especially in the interstitial lymphocytes infiltration and antigen presenting or as a target for the effector arm of the reaction.
5.Study on the physiological changes of the late-onset depression during sleep
Xiuying XING ; Jing XU ; Junping WANG ; Mi ZHOU
Chinese Journal of Behavioral Medicine and Brain Science 2011;20(2):131-133
Objective To explore ihe physiological changes of the late-onset depressive(LOD) persons during sleep.Methods 15 cases of LOD patients formed a group and 10 healthy aging persons formed a control group.Hamilton Depression Scale (HAMD) was used to score the severity of depression and polysomnographic recorders were used to monitor the whole night long.The subjective feelings of the sleep and the daytime mental status were assessed in the morning.Results Compared with the controlde group, the LOD patients obviously possessed a disordered sleeping process: sleep efficiency was lower( ( 59.20 ± 2.90 ) %, ( 77.09 ± 1.55 ) %, P <0.01 ); their sleep latency was longer( (54.00 ± 4.97 ), ( 24.00 ± 2.91 ), P < 0.01 ); the numbers of rapid eye movement(REM) sleep phase and rapid eye movement latency(REML) were strikingly different(P<0.01 ).Compad with the control group, the LOD patients' awakening time and percent were significantly increased.Not only were the time and percentage of S2 sleep phase much higher, but the time of slow wave sleep was shorter.Besides,REM sleep activity, its density, and its intensity were raised (P < 0.05 ).Conclusion There is a disturbance of the objective physiological indexes happening among the LOD patients.The increase of S2 sleeping time and percentage and the disinhibition of REM sleep can be used as the diagnosis indexes of LOD patients' specificity.
6.Adrenal myelolipoma(report of 6 cases)
Junping XING ; Runming LIU ; Jiangong DANG ; Al ET
Chinese Journal of Urology 2000;0(01):-
Objective To study the clinical and pathological features of adrenal myelolipoma. Methods The clinical manifestration,experimental and imaging data of 6 patients with adrenal myelolipoma were retrospectively analyzed. Results The disease manifested nonspecific symptoms and signs,including faint lumber,flank or bulk pain.The diagnosis of adrenal myelolipoma depends on B ultrosound scan,CT and MRI,revealing a fatty mass with various other densities such as soft tissue,intratumoural haemorrhage,calcification and residual adrenal cortex.Tumor excision and adrenalectomy were performed in all patients.The pathological examination showed that the tumor consists of mature fat cells and hematopoietic elements.The patients have been asymptomatic on following up for 0.5~7 years and there is no recurrence of tumor. Conclusions CT and MRI images features are helpful for the pre operative diagnosis of adrenal myelolipoma.Pathologically,it is characterized by a mixture of mature fat and hematopoietic elements.
7.Plasma level of salusin-? in patients with coronary heart disease and its clinical implications
Jianbo SHI ; Xing ZHENG ; Xiaosong GU ; Junping LEI
Academic Journal of Second Military Medical University 2000;0(08):-
Objective To explore the relationship of plasma salusin-? level with the stability,severity,and other risks of coronary atherosclerosis.Methods The patient group included 122 hospitalized patients with coronary artery disease(CAD),whose diagnoses were confirmed by coronary angiography(CAG).The CAD group was further divided into subgroups according to the clinical types,the number of diseased coronary branches,and Gensini's scores.Control group inlcuded 60 healthy subjects who underwent physical examination in our hospital.Salusin-? level and other general biochemical indicators were determined,and the general clinical data were obtained before CAG in all subjects.Results The peripheral blood salusin-? level in CAD patients was significantly lower than that in the controls([0.50?0.18]ng/ml vs [0.69?0.23 ng/ml],P
8.Correlation between Glutathione S-transferase Polymorphisms and Sperm DNA Integrity in Male Patients with Idiopathic Infertile
Huan WANG ; Fa SUN ; Junping XING ; Shangshu DING ; Chao SUN ; Xinyang WANG ; Tiejun ZOU ; Kaifa TANG
Journal of China Medical University 2015;(12):1075-1078
Objective to investigate the correlation between glutathione S- transferase gene M1,t1 and P1(GStM1,GStt1 and GStP1)poly-morphism and sperm DNA fragmentation index(DFI)in male patients with idiopathic infertile. Methods the study included 246 male patients with idiopathic infertility. Polymerase chain reaction(PCR)and polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP) were used to identify the genotype of GStM1,GStt1 and GStP1,respectively. Sperm DFI was analyzed by flow cytometry with acridine orange staining. Results the sperm DFI was significantly higher in GStt1 gene null type group than in GStt1 gene wild type group(P < 0.01);and there were no significant differences in GStM1 and GStP1 gene mutation type groups when comparing with the wild type group(P > 0.05). Conclusion GStt1 gene deletion was positive associated with the sperm DFI in male idiopathic infertile patients.
9.Effects of Beta-asarone on Hippocampal Neurons of Depression Rat Model
Junping WANG ; Xiaojie ZHANG ; Xiuwen YU ; Guihua XING ; Yurong SUN ; Wei RONG
Journal of Guangzhou University of Traditional Chinese Medicine 2014;(6):924-927
Objective To investigate the effect of β-asarone on hippocampal neurons of experimental depression rats. Methods Eighty male adult SD rats were randomly divided into 4 groups, namely normal group, model group, fluoxetine(1.2 mg/kg) group and β-asarone (25 mg/kg) group. Depression rat model was established. The medication groups were given corresponding agents respectively. After treatment for 21 days, the number of apoptotic cells and the histological features in the hippocampus of rats were detected by flow cytometry and TUNEL, respectively. Results Compared with the normal group, typical apoptotic cells were found, apoptotic cell count and apoptotic rate were increased in the hippocampal CA3 and DG area of the model group (P<0.01). Compared with the model group, apoptotic cell count and apoptotic rate were decreased in the hippocampal area ofβ-asarone group and fluoxetine group (P<0.01). Conclusion The protective mechanism of β-asarone for the hippocampal cells of depression rats is probably associated with the reduction of the apoptosis of hippocampal neurons.
10.In vitro effect of total flavones of Fructus Chorspondiatis on expression of collagen type I and type III mRNA and protein of cultured rat cardiac fibroblasts.
Junping BAO ; Ming JIN ; Yumin YANG ; Xiaohui GAO ; Liang SHU ; Huihui XING ; Lei JIA
Acta Pharmaceutica Sinica 2014;49(1):136-41
This study aims to investigate the effect of total flavones of Fructus Chorspondiatis (TFFC) on the mRNA and protein expression of collagen type I and III of rat cardiac fibroblasts (CFs) induced by angiotensin II (Ang II), and explore its anti-myocardial fibrosis molecular mechanism. Neonatal rat CFs were prepared from Sprague-Dawley rats (1-3 d after birth). The expression of collagen type I and III mRNA and protein were measured by RT-PCR and Western blotting, respectively. The study showed that stimulation of neonatal rat CFs with 100 nmol.L-1 of Ang II for 72 h resulted in a significant increase of the expression of collagen type I and III mRNA and protein. The changes on the expression level were blocked by TFFC. The results demonstrated that TFFC can inhibit myocardial fibrosis induced by Ang II in rats, which is probably associated with the collagen type I and III mRNA and protein levels up-regulated by Ang II, and TFFC was shown to decrease the expression levels of collagen type I and III mRNA and protein.