Objective To investigate the effects of the locking compression plates on the intra-articular calcaneal fracture.Methods The lateral foot incision and locking plate was used for treatment of calcaneal fracture 54 cases.Results 36 patients were followed average 18 months(6-36months).The overall results to the Maryland Scale were evaluated as excellent in 26 cases,good in 5 cases.The rate of excellent and good was 86％.Conclusion The internal fixation with locking compression plate in treatment of calcaneal fractures can achieve good clinical results.It is a kind of ideal treatment method.

The effects of PGE2 on glucose metabolism in alloxan-induced diabetic and normal rats were studied by subcutaneous injection of PGE2, with the serum levels of glucose, insulin and glucagon, and histopathological survey of pancreas as indices of assessment. The results revealed that the cytoprotective effect of PGE2 against the damage of pancreatic islets caused by alloxan was invisible, however, a slight reduction of serum glucose level, suppression of insulin secretion and stimulation of glucagon secretion were observed. The hypoglycemic effect of PGE2 in alloxan-induced diabetic rats seemed to be dosage dependent and probably related to temporary hyperglycemia and inhibiton of glucagon on glycogenolysis in liver. Thus, it might be suggested that PGE2 has no cytoprotective effect on pancreatic islets against alloxan damage, but is rather an important factor in regulating insulin and glucagon secretion as well as serum glucose level both in normal and alloxan-induced diabetic rats.

Objective To construct a recombinant AAV reporter virus expressing GFP and observe its expression in NIH 3T3 cell. Methods The resulting gene of GFP was subcloned into the site EcoRⅠand BamHⅠof vector pssHGCMV, and a recombinant adeno-associated virus (rAAV) vector pssHGCMV-GFP expressing GFP was constructed by using DNA recombinant technique. The 143 cells were transfected with the vector and the rAAV stock expressing GFP was packaged, produced and purified. The titre of the rAAV stock was measured by quantitative dot blot hybridization. NIH 3T3 cells were infected with the rAAV and the fluorescence was detected in alive cells to confirm successful infection and the expression of exogenous gene in NIH 3T3 cell line. Results A GFP-expressing rAAV vector VssCMV-GFP was successfully constructed. The titer of the VssCMV-GFP was 3.16?10~(12) particles/mL. GFP was initially expressed in the NIH 3T3 cells 48h following infected by VssCMV-GFP and subsequently it gradually increased. The green fluorescence in the cells was the most apparent 120h after infected. Conclusion VssCMV-GFP can infect the eukaryotic cells and GFP can be expressed in the alive cells.It is suggested that rAAV-mediated transfer of the gene to the eukaryotic cells can serve as a delivery system of gene therapy.

The evaluation systems of higher education in such developed countries as USA and Japan,are perfect and systemic,and it is significant to analyze them and use the experiences for reference.

[Objective] To understand the mental patients with tracheal intubation, formulate the corresponding nursing intervention, improve the quality of nursing care.[Methods] Analysis of extracting some nursing care of patients with tracheal intubation cases, discuss the causes of psychological problems and intervention measures.[Results] The tracheal intubation patients by nursing intervention, man-machine confrontation, accidental y were put the tube, with-out the treatment of reduction. [Conclusion] Take corresponding nursing intervention according to the psychological problems of patients with tracheal in-tubation, reduce complications during tracheal intubation occurred, improve the mental health status of patients, improve the nurse-patient communica-tion, improve the quality of nursing.

OBJECTIVE:To prepare erchen dispersible tablet and establish its quality control method.METHODS:cCMC-Na was used as disintegrating agent,and polyvinyl pyrrolidone as well as other adjuvants were added to prepare the tablet;The content of hesperidin was determined by HPLC,and the stability of the finished product was also evaluated.RESULTS:The sample size of hesperidin was linear in the range of1?g～80?g,the average recovery was99.98%,RSD=0.98%;The preparation showed good stability.CONCLUSION:The present formulatiom is rational,and the preparation technic is simple,the finished product meets the standards of Chinese Pharmacopenia.

Hand, foot, and mouth disease (HFMD) is a viral infectious disease regarded to be a public-health problem worldwide. Since the 1990s, HFMD began to spread in the Asia-Pacific region (especially in South-East Asia). HFMD outbreaks have occurred in mainland China frequently since 2008, and the morbidity and mortality of HFMD has continued to increase in recent years. In mainland China, enterovirus A serotype enterovirus A71 (EV-A71) and coxsackievirus A16 (CVA16) have been the major pathogens of HFMD during these years. However, the etiological spectrum of HFMD changes with time. This review focuses mainly on the etiological spectrum of HFMD and changes in epidemic patterns in mainland China.
China ; epidemiology ; Disease Outbreaks ; Enterovirus ; classification ; genetics ; isolation & purification ; Hand, Foot and Mouth Disease ; epidemiology ; virology ; Humans ; Prevalence

Fiber type of skeletal muscle and its multiple and specialized protein isoform are the structure and molecular basis of the skeletal muscle's function and adaptability.Myosin heavy chain(MHC)isoform,as the factor determinating the type of the skeletal muscle to be fast or slow one,has become molecular biomarker of muscle fiber type and adaptability.Exercise may lead to the transform between myosin subtypes.This article reviewed not only the relation between myosin heavy chain and muscle fiber type of skeletal muscle,but also the influences of different motor patterns' on the transform of skeletal muscle fiber MHC isoform.

This article pointed out that the Suiqisuode theory was the basic pathogenesis of viral myocarditis by analysing that theory.

Cardiovascular disease in peri-menopausal period is a kind of climacteric syndrome which based on the symptoms of the cardiovascular system as the main manifestations. It is manifested as hypertension,pseudo-angina,arrhythmia. Hormone replacement therapy or symptomatic treatment is the common used method of modern medicine,although a certain degree of efficacy,but there are also significant disadvantages. In the treatment of cardiovascular disease in peri-menopausal period,TCM has certain advantages. In this paper,we treat cardiovascular disease in peri-menopausal period from the theory of 'yin deficiency,blood stasis,heat-toxin'and propose the method of 'nourishing yin,promoting blood circulation and detoxification'.