1.Enlightenment of the Higher Education Evaluation Systems in USA and Japan
Chinese Journal of Medical Education Research 2005;0(05):-
The evaluation systems of higher education in such developed countries as USA and Japan,are perfect and systemic,and it is significant to analyze them and use the experiences for reference.
2.Tracheal Intubation in Patients with Common Psychological Problems and Nursing Intervention
Journal of Zhejiang Chinese Medical University 2013;(11):1356-1357
[Objective] To understand the mental patients with tracheal intubation, formulate the corresponding nursing intervention, improve the quality of nursing care.[Methods] Analysis of extracting some nursing care of patients with tracheal intubation cases, discuss the causes of psychological problems and intervention measures.[Results] The tracheal intubation patients by nursing intervention, man-machine confrontation, accidental y were put the tube, with-out the treatment of reduction. [Conclusion] Take corresponding nursing intervention according to the psychological problems of patients with tracheal in-tubation, reduce complications during tracheal intubation occurred, improve the mental health status of patients, improve the nurse-patient communica-tion, improve the quality of nursing.
3.Construction of GFP reconbinant AAV- reporter virus and its expression in NIH 3T3 cell
Journal of Xi'an Jiaotong University(Medical Sciences) 1982;0(04):-
Objective To construct a recombinant AAV reporter virus expressing GFP and observe its expression in NIH 3T3 cell. Methods The resulting gene of GFP was subcloned into the site EcoRⅠand BamHⅠof vector pssHGCMV, and a recombinant adeno-associated virus (rAAV) vector pssHGCMV-GFP expressing GFP was constructed by using DNA recombinant technique. The 143 cells were transfected with the vector and the rAAV stock expressing GFP was packaged, produced and purified. The titre of the rAAV stock was measured by quantitative dot blot hybridization. NIH 3T3 cells were infected with the rAAV and the fluorescence was detected in alive cells to confirm successful infection and the expression of exogenous gene in NIH 3T3 cell line. Results A GFP-expressing rAAV vector VssCMV-GFP was successfully constructed. The titer of the VssCMV-GFP was 3.16?10~(12) particles/mL. GFP was initially expressed in the NIH 3T3 cells 48h following infected by VssCMV-GFP and subsequently it gradually increased. The green fluorescence in the cells was the most apparent 120h after infected. Conclusion VssCMV-GFP can infect the eukaryotic cells and GFP can be expressed in the alive cells.It is suggested that rAAV-mediated transfer of the gene to the eukaryotic cells can serve as a delivery system of gene therapy.
4.Surgical treatment for intra-articular calcaneal fracture
Chinese Journal of Primary Medicine and Pharmacy 2013;20(15):2329-2330,后插2
Objective To investigate the effects of the locking compression plates on the intra-articular calcaneal fracture.Methods The lateral foot incision and locking plate was used for treatment of calcaneal fracture 54 cases.Results 36 patients were followed average 18 months(6-36months).The overall results to the Maryland Scale were evaluated as excellent in 26 cases,good in 5 cases.The rate of excellent and good was 86%.Conclusion The internal fixation with locking compression plate in treatment of calcaneal fractures can achieve good clinical results.It is a kind of ideal treatment method.
5.Preparation and Quality Control of Erchen Dispersible Tablet
China Pharmacy 2005;0(14):-
OBJECTIVE:To prepare erchen dispersible tablet and establish its quality control method.METHODS:cCMC-Na was used as disintegrating agent,and polyvinyl pyrrolidone as well as other adjuvants were added to prepare the tablet;The content of hesperidin was determined by HPLC,and the stability of the finished product was also evaluated.RESULTS:The sample size of hesperidin was linear in the range of1?g~80?g,the average recovery was99.98%,RSD=0.98%;The preparation showed good stability.CONCLUSION:The present formulatiom is rational,and the preparation technic is simple,the finished product meets the standards of Chinese Pharmacopenia.
6.EFFECTS OF PGE_2 ON CARBOHYDRATE METABOLISM IN ALLOXAN-INDUCED DIABETIC AND NORMAL RATS
Chinese Journal of Endocrinology and Metabolism 1986;0(04):-
The effects of PGE2 on glucose metabolism in alloxan-induced diabetic and normal rats were studied by subcutaneous injection of PGE2, with the serum levels of glucose, insulin and glucagon, and histopathological survey of pancreas as indices of assessment. The results revealed that the cytoprotective effect of PGE2 against the damage of pancreatic islets caused by alloxan was invisible, however, a slight reduction of serum glucose level, suppression of insulin secretion and stimulation of glucagon secretion were observed. The hypoglycemic effect of PGE2 in alloxan-induced diabetic rats seemed to be dosage dependent and probably related to temporary hyperglycemia and inhibiton of glucagon on glycogenolysis in liver. Thus, it might be suggested that PGE2 has no cytoprotective effect on pancreatic islets against alloxan damage, but is rather an important factor in regulating insulin and glucagon secretion as well as serum glucose level both in normal and alloxan-induced diabetic rats.
7.Progress in molecular targeting therapy for gastrointestinal stromal tumors
Chinese Journal of Cancer Biotherapy 2019;26(3):253-259
Gastrointestinal stromal tumors (GISTs) are the most common malignant tumor of abdominal soft tissue. It originates from Cahal (Cajal) interstitial cells or common precursor cells, and is driven by the mutated KIT gene or platelet-derived growth factor receptor alpha (PDGFRa) gene, all expressing type Ⅲ tyrosine kinase receptors. Imatinib mesylate, a tyrosine kinase receptor inhibitor, has been used for the standard treatment of advanced GIST, which has achieved remarkable results. Thus, GIST has become the most successful example of target therapy for solid tumors. In the context of the era of precision medicine, with the deepening in research of GISTs molecular biology,the molecular targeted treatment of GISTs has obtained a clear venation from the first-line, second-line and third-line of the advanced stage to the postoperative auxiliary and preoperative treatment, providing significant survival benefits for GISTs patients. This article systematically and comprehensively combed the preoperative and postoperative molecular targeting therapy from advanced GIST to early GIST, and analyzed the problems, proposed solutions and prospects for the future, aiming to provide reference for clinical application of molecular targeting drug therapy for GIST.
8.Pathogenesis and prevention tactics of aspirin resistance
Journal of Integrative Medicine 2007;5(3):259-62
Aspirin (acetylsalicylic acid) is a nonsteroidal anti-inflammatory drug. Despite its wide uses for more than 100 years, knowledge about mechanism of action and therapeutic issues of aspirin are still under discussion. The use of aspirin has been changed from an analgesic, anti-pyretic and anti-inflammatory agent to an anti-thrombotic agent, especially in secondary prevention of cardiovascular events. Aspirin has reduced the risk of cardiovascular events by 25%. However, the phenomenon of "aspirin resistance" has been described that in 5%-60% of patients aspirin may not achieve adequate efficacy of suppressing platelet activity. The convinced causes of this phenomenon are still unknown. It is probably due to drugs interaction, inadequate dosage and so on. By far the existing studies of aspirin are insufficient to explain all phenomena of aspirin resistance. And the results are not always uniform about the same research. Therefore, the characteristics in different population with aspirin resistance may account for the complexity. It is unrealistic to elucidate all aspirin resistance by only one pathway. More studies are required to investigate the mechanisms in different population respectively. According to the theory of traditional Chinese medicine and the trait of cardiovascular disease, which often relapses and has a long history, aspirin resistance should be considered as collaterals disease. It can be treated with aspirin and traditional Chinese drugs which have the power to strengthen body resistance, reduce phlegm, remove blood stasis and toxic materials from meridians. The problem of aspirin resistance might be solved by this way, because the traditional Chinese medicine has the superiority of selecting appropriate therapeutic methods based on syndrome differentiation for different population and regulating the whole body's function. Subsequently, cardiovascular disease might be effectively prevented.
9.NKG2D and its ligands in tumor immunotherapy
Journal of International Oncology 2011;38(5):349-351
Natural killer group 2,member D (NKG2D) is a C-typelectin-like activating receptors on natural killer cells surface. NKG2D engaging with the ligands on tumor cells surface can eliminate tumor cells. However,there must exist immune escape mechanisms in tumor mice and cancer patients. Recently, molecular biological techniques are used to regulate the expression of the receptors and ligands to break the immune evasion.
10.Ideas of TCM in treating viral myocarditis with arrhythmia
International Journal of Traditional Chinese Medicine 2011;33(6):527-529
Viral myocarditis involving the cardiac conduction system is easily associated with a variety of arrhythmias. Negative inotropic effect and the potentially induced arrhythmia of antiarrhythmic drugs limit their application. Non-drug treatment with the body injuries and high medical costs holds patients back and leads them to take a conservative treatment. Traditional medical treatment of this disease has a long history and rich experience. Clinical and experimental studies show that traditional Chinese medicine is effective and has few side effects. This article discusses the ideas of treatments of arrhythmia, from three aspects of differentiating diseases and syndrome type, dispelling wind-evil and dredging collaterals and using results of pharmacological researches reasonably.