Autism is a pervasive developmental disorder characterized by deficits in socialization, communication, and adaptive functioning. To investigate its neurophysiology, lots of measures have been used. In the past twenty years, due to its evaluation function in the central auditory processing and recognition, auditory event related potential has been implemented in autism more and more generally.

Objective: To study the relationship between endoplasmic reticulum stress (ERS) and adiponectin;to explore the role of ERS for increasing myocardial ischemic vulnerability in type 2 diabetes mellitus (DM) mice.
Methods: Type 2 DM model was established by high fat diet with streptozotocin (STZ) injection. A total of 35 C57BL/6J male type 2 DM mice were divided into 4 groups: ①Control group, n=5. ②Tauroursodeoxycholic acid (TUDCA) group,③Thapsigargin (TG) group and ④Normal saline group. The mice in Groups ②, ③, ④were fed by high fat and high glucose diet by injecting streptozotocin (STZ), in the last 3 weeks and respectively received intraperitoneal injections of TUDCA (250 mg/kg), thapsigargin (TG) (300μg/kg) and normal saline twice a day, n=10 in each group. Then myocardial infarction (MI) model was established in 5 mice from each group. 72 hours later, the MI ranges were measured, serum levels of adiponectin were detected, mRNA expressions of adiponectin and CHOP in myocardial tissue were examined.
Results: The MI range in TUDCA group (21.47 ± 2.85)%and in Normal saline group (39.92 ± 4.28)%were both lower than TG group (66.56 ± 8.15)%, both P<0.01. Before MI occurrence, serum levels of adiponectin in TUDCA group (79.25 ± 6.40) pg/ml and in Normal saline group (70.23 ± 4.15) pg/ml were both higher than TG group (62.64 ± 5.70) pg/ml, both P<0.01;serum levels of adiponectin in each group were higher than they were 72 h after MI. In each group, the mRNA expression and protein content of adiponectin in myocardial tissue were constant to serum adiponectin;while the mRNA expression and protein content of CHOP was opposite to serum adiponectin.
Conclusion: ESR could increase myocardial vulnerability in type 2 DM mice which might be related to down-regulating adiponectin expression.

Objective To observe the influence of self-management on health care behavior through health education for patients with hypertension after a self-management model was brought to the communities. Methods There are 252 cases chosen randomly from 546 hypertensive patients in 19 communities to participate in the standard management of hypertension in communities voluntarily. They improve their self-management skills through attending lectures, the guidance of healthy lifestyle and self-management manuals. A scientific self-management methods was established and the support of the society and family was improved. The self-control method was used to analyze this study. Results There are significantly differents (P<0.05 ) in basic health knowledge understanding, self-management skills, and blood pressure control after health education compared with before. Conclusions Patients with health education can improve the self-management level. There are obvious effects on changing the bad lifestyle,improving blood pressure control rate, and decreasing the complication of patients with hypertension.

Objective To explore the characteristics of deficit in action imitation upon objects for the children with autistic spectrum disorder(ASD). Methods Imitative tasks involved objects under the condition of different time after the modelling action ,different action number, different action meaning were used to examine the variations between 12 young children with ASD,and with deaf children(D) matched with the non-verbal IQ and CA( n= 12) ,and children with mental retardation (MR) matched with verbal IQ and CA( n= 12). Results Analyses of repeated ANOVA revealed that: there were significant main effect on the imitation of action involved objects within three subjects(F(2.33) = 10. 138, P＜0.01 ) ;the individuals with ASD achieved lower scores(0.766 ±0.029 ) than the children with MR (0.861 ± 0. 029 ), and the children with deaf significantly ( 0. 949 ± 0. 029). Especially,the autistic imitation deficit was shown on tasks of deferred and meaningless action imitation. Conclusion Children with ASD show deficit on imitation involved objects significantly.

Both molecular weight 6000 and 1000 polyethylene glycol (PEG) have been used to induce thc fusion of hepatoma cell by two methods of centrifugation and stillstand. The results showed that 50% PEG of molecular weight 1000 and centrifugation increase the percentage of fused cells. The procedure of cell fusion has been examined. The cytoplasm has been fused when the cells start fusing in one hour and a half, and the neucleus start fusing in two horus and a half.

OBJECTIVE To evaluate the pharmacological actions of the Formulae of Qinwenbaiduyin and Huanglianjiedutang in antibacterial experiment in vitro. METHODS Test tube method and plating method were employed in antibacterial experiment while test was conducted in antiviral experiment with extract of the Formula by water and then observing the pathological changes in the lungs after medication. RESULTS The minimal inhibirory concentration (MIC) effect of Qinwenbaiduyin was 15.6 mg/ml, the minimal bacteriocidal concentration (MBC) was 31.2mg/ml to Staphylococcus aureus; minimal inhibirory concentration (MIC) effect was 7.6mg/ml, MBC was 15.6mg/ml to Escherichia coli. The MIC effect of Qinwenbaiduyin was 15.6 mg/ml, the MBC was 31.2mg/ml to S. aureus and E. coli. CONCLUSIONS The Formulae of Qinwenbaiduyin and Huanglianjiedutang have antibacterial effect on S. aureus and E. coli in experiment.

Objective To study the effect of antivirus therapy of HBV reinfection and YMDD mutation after liver transplantation. Methods Fifteen of 317 patients with HBV-related end-stage liver diseases received lamivudine ( LAM ) monothereapy, others received combination low-dose hepatitis B immune globulin( HBIG) and LAM (or adefovir dipivoxil, ADV) therapy, as prophylaxis against HBV reinfection after OLT. Hepatitis serum markers, HBsAg, HBeAg, HBcAb-IgM, and HBcAg were detected every 2 weeks by immunohistochemistry. Serum HBV DNA was examined by PCR every 2 weeks. HBsAg and HBcAg in the liver specimens were examined by immunohistochemistry. YMDD mutation was detected by PCR in those patients with recurrence of positive HBV DNA posttransplantation. Results In LAM monotherapy group, 4 developed HBV reinfection out of 15 patients with pretreatment positve HBV DNA. Sixteen of 302 patients with combination HBIG and LAM therapy suffered from posttransplant HBV reinfection, the difference between the two groups was significant (26.7% vs. 5. 30% ,P

Objective: To study the protective effect of compound extract of soybean, wolfberry and hawthorn on chemical liver injury in mice respectively. Methods:The mice were randomly divided into 5 groups:control(A), liver injury control(B)and 0.06(C)?0.20(D)?0.60(E) g/(kg?bw) compound extract group. The liver injury models were induced by CCl 4 and ethanol in mice respectively. After 4 weeks, for the former, the serum ALT and AST content were measured and the liver pathological change was observed. For the latter, the liver MDA, GSH, and TG content were measured and fat droplets staining in the ice pathological slice were observed . Results: 1.CCl 4 liver injury models:the content of serum ALT of group D and the AST of group D and E were decreased. Compared with normal group, the hepatic pathological changes of the compound extract group was mainly fatty and ballon degeneration, and that of the CCl 4 group was mainly hepatic cytoclasis. 2.Ethanol liver injury models: the content of liver MDA of group C, E and the TG of group C, D, E were all decreased, and the fatty degeneration scores of group C, D were lower, both compared with ethanol group. Conclusion: Compound extract of soybean, wolfberry and hawthorn has the protective effect on liver injuries induced by both CCl 4 and ethanol in mice.

ObjectiveTo study the effect of anterior decompression and autograft fusion under video-assisted thoracoscopic to treat lower thoracic vertebrae bursting fracture complicated with intervertebral disc injury.MethodsEleven patients who suffered from lower thoracic vertebrae bursting fracture complicated with intervertebral disc injury were treated with anterior decompression and autograft fusion under video-assisted thoracoscopic from December 2005 to May 2008.The involved vertebrae included T12 in 5cases,T11 in 4 cases,T10 in 1 case and Ts in 1 case.According to the AO classification,4 patients were A2.2and 7 were A3.According to the Frankel classification,5 patients were rated as grade A,2 as grade C,2 as grade D and 2 as grade E.ResultsEvery patient underwent successful operation.The operation time was 3.5-8 h(average,5.2±1.6).The blood loss was 600-3800 ml(average,1195±576).One patient got intercostal neuralgia after operation,which disappeared after treating with analgesic drugs for 7 days.All patients were followed up for 36-65 months (average,49.5±5.9).All patients got bony fusion according to the CT scans 24month after operation.There were no neurological function deterioration and other instrument complications happened.At the last follow-up,5 patients were rated as grade A,2 as grade D and 4 as grade E,according to the Frankel classification.ConclusionAnterior decompression and autograft fusion under video-assisted thoracoscopic is an effective method to treat lower thoracic vertebrae bursting fracture complicated with intervertebral disc injury.However,this method needs higher technology and has a long study-curve.

Objective Acute type A aortic dissection associated pregnancy severely threatens the lives of both the mother and her ferus.We retrospectively reviewed our clinical experience with this life-threatening condition in six cases.Methods Between January 2007 and February 2012,6 women with acute type A aotic dissection associated pregnancy were treated by our group,with an average of 3 1 years (range 24 -37 weeks)and a mean gestation weeks of 24.5 (range,12 -38 weeks ).The etiology was Marfan syndrome in 4 cases and gestational hypertension in 2.The pathology was the modified Stanford type A3S in I case,A2C in 2 and A3C in 3.- Five patients were treated surgically and 1 medically.Surgical operations were performed under hypothermic cardiopulmonary bypass or deep hypothermic circulatory arrest,including Bentall procedure in 1case,Bentall + Sun's procedure in 2,ascending aortic replacement + Sun's procedure in 2.Results The woman treated medically and her fetus died from aortic rupture 9 days after admission.The cardiopulmonary bypass and cross clamp time and circulatory arrest time averaged 167 rninites(range,75 -210 minites) and 98 minites(range,83 - 145 minites) and 23.5minites(range,19 -27 minutes),respectively.Five patients treaed surgically survived the operation.Three fetuses survived rand two fetuses died.After a mean follow-up of 2.2 years (range,1 - 3.5 years ),5 patients were doing well.CT angiogram detected nonmal aortic and valvular structures,with no signs of distal dilation.Three babies were normal in development and neurocognitive functios.Conclusion Palients with aortic dissection associated with pregnancy should be operated on ugently and medical treatment carries high risks of aortic rupture and maternal and fetal death.Methods of surgical repair,peffusion techniques and delivery should be chosen based on the underlying aortic pathology and gestational age,so as to maximize the safety of the mother and her baby.