The total soil cultured bacterial DNA was extracted by improved CTAB -lysosome-protease K method and was analyzed by random amplified polymorphic D NA markers. The effect of concentration of Mg 2+ , dNTP, DNA templates, pri mers, DNA polymorase and bovine serum albumin on experimental results were teste d and the optimal reaction system of RAPD for soil cultured bacteria was determi ned.

OBJECTIVE: To probe into the function of early exercises on healing of grafts after anterior cruciate ligament (ACL) reconstruction and on motor function recovery of knee joint by summarizing biology and biomechanical properties of ACL reconstruction.DATA SOURCES: The relevant literatures on ACL reconstruction of knee joint and its biomechanical properties were looked up in period of MEDLINE1970-01/2004-01 on computer. Retrieval words: "anterior cruciate ligament, knee joint, biomechanics" only in English.STUDY SELECTION: In initial examination, the complete contents of literatures on ACL reconstruction of knee joint and its biomechanical properties were selected. The controlled papers were randomized, for those written by the same writer, the papers with the most cases were included.Comprehensive and Mete analyzed papers were excluded. Quality evaluation was mainly based on the reality of observed data and rigor of investigation and application.DATA EXTRACTION: Fourty-six papers on ACL reconstruction of knee joint and its biomechanical properties were looked up totally, of which, 29papers were tallied with inclusive criteria, 17 papers were excluded, 9 papers were the analysis on small samples and 8 papers referred to comprehensive and Meta analysis.DATA SYNTHESIS: By understanding biomechanical properties of normal ACL it is to implement reconstruction measures and plan for rehabilitation. Animal experiment after ACL reconstruction suggests that the motor function recovery of knee joint is not satisfactory, but the results of human experiment are superior to animal one. It is indicated in randomized control experiment that immediate weightbearing right after ACL reconstruction will not aggravate knee joint laxity; on the contrary, it is advantageous to alleviate patellar pain.CONCLUSION: Reconstruction of injured ACL fixes knee joints, limits peripheral muscle contraction and results in limited motor function of ligaments, joints and peripheral muscles. Early rehabilitation exercises may alleviate pain, improve articular cartilage metabolism, prevent capsule contracture and benefit recovery of motor function after ACL reconstruction of knee joint.In randomized comparisons of Bear[27], Fischer[28] and Beynnon[29],there is no difference between rehabilitation at home and in hospital after ACL reconstruction with bone-patellar-bone graft.

Radioimmunotherapy using monoclonal antibodies incorporated with radionuclide has been showed to be an effective agent for refractory non-Hodgkin's lymphoma (NHL). Many anti-CD20 antibodies labeled with radionuclide, such as 90Y-ibritumomab tiuxetan (Zevalin) and 131I-tositumomab tiuxetan (Bexxar), have been reported to be effective for the treatment of patients with relapsed or refractory low-grade, follicular, or transformed NHL. This review summarizes the current advance in clinical trials and studies of Zevalin and Bexxar for the treatment of NHL patients.

BACKGROUND:Type 2 diabetic patients suffered from different levels of insulin resistance and insulin secretion defects. Retinol binding protein 4 is a fat derived factor found in the blood and is considered tobe an important target for the treatment of visceral obesity and insulin resistance.
OBJECTIVE:To observe the level of serum inflammatory cytokines and the changes of immune function in insulin resistance model rats after swimming.
METHODS:A total of28 8-week-old Sprague-Dawleymale ratmodels were randomly divided into swimming group and rest group. Rat received intraperitoneal injection with 3 μg/g recombinant serum retinol binding protein 4, twice a day, with an interval of 12 hours, for 6 weeks. At 6 weeks after administration, rats in the swimming group were subjected to swimming without loading for 6 weeks, 60 minutes per day. Serum retinol binding protein 4 levels were measured with enzyme linked immunosorbent assay (ELISA) kit. Insulin sensitivity (insulin resistance index) was assessed with homeostasis model assessment. Terminal deoxynucleotidyl transferase dUTP nick end labeling method was used to mark the apoptotic cels in rat thymus. ELISA was used to detect serum interleukin-6 expression. ABC-ELISA was used to test Level of serum granulocyte macrophage colony stimulating factor. S-P One Step Method was used to test CD4 and CD8 activities in blood.
RESULTS AND CONCLUSION:Interleukin-6 and granulocyte macrophage colony stimulating factor were significantly positively associated with insulin resistance index. The increase in insulin resistance index would induce the increase in the levels of serum inflammatory cytokine interleukin 6 and granulocyte macrophage colony stimulating factor, but exercises could decrease above levels. Thymus index, CD4, and CD4/CD8 were negatively associated with insulin resistance index. These results indicate that exercise intervention effectively improved immunity function of rats with insulin resistance and reduced insulin resistance index.

Objective To investigate relationship of the marrow blast decline index(MBDI)in AML (not APL)at the first inductive treatment for 7-10 days and the prognosis.Methods 109 cases of AML were retrospectively analyzed(not APL),to compare theⅡmarrow blast decline index in AML(not APL)at the first inductive treatment for 7-10 days in the continue relieve group,the relapse group and the non-remission group,analyzed the correlation of the MBDI and continue relapse rate,the MBDI and relapse rate.Results The MBDI mean value is 0.8856 in continue relieve group.significantly higher than that of the relapse group (mean=0.4445)and the non-remission group(mean=0.3903)(P<0.0001).The MBDl positively correlated with continue relapse rate(P<0.01);negatively correlated with relapse rate(P<0.01).Conclusion The marrow blast decline index in AML(not APL) at the first inductive treatment for 7-10 days was significant for early assesment ofthe prognosis.

Objective To investigate the clinical,safety and cost effect of porcine pulmonary surfactant (import)and bovine pulmonary surfactant(domestic)with full dose and low dosage of early treatment for late preterm children with respiratory distress syndrome,for the clinical selection treatment programs provide a reference.Methods Late preterm children with respiratory distress syndrome were divided into full dose group A of 24 cases,low dose group A of 25 cases,full dose group B of 26 cases,low dose group B of 24 cases.The first dose of full dose group A was 200mg/kg,the first dose of low dosage group A was 100mg/kg,again administered doses was 100mg/kg,the first dose of full dose group B was 70mg/kg,the first dose of low dosage group B was 50mg/kg,again administered dose was 50mg/kg.Results The differences of PaO2 ,PaCO2 ,FiO2 ,OI between two low dosage groups and full dose group A were significantly different.Treated 6h,the differences of PaCO2 ,FiO2 between low dosage group B and full dose group A were significantly different(q =3.273,3.236,all P <0.05).A group of low volume with a sufficient amount PaO2 6 h B group differences were statistically significant(q =3.823,P <0.05),12h of PaO2 ,PaCO2 ,FiO2 ,OI with enough B group difference was statistically significant(q =3.293,3.923,3.423,3.434,all P <0.05),24h of FiO2 , OI group was significantly lower than the full amount of B(q =3.234,3.356,all P <0.05).Low dose group B 6h, 12h,24h of PaO2 was significantly lower than enough group B,6h,12h of PaCO2 were significantly higher than enough group B(q =3.892,3.922,3.583,all P <0.05),12h,24h of FiO2 ,OI group were significantly higher than a suffi cient amount B(12h of q =3.892,3.762,24h of q =3.819,3.678,P <0.05),24h of OI was significantly higher than the low amount of a group(q =3.784,P <0.05).Chest evaluation 12h,24h grade difference between groups was sta-tistically significant(Z =3.982,5.323,all P <0.05).Two low -volume set of ventilator -associated pneumonia rates were 16.00%,16.67%,significantly higher than the amount of the two -legged group(low dose group A χ2 =4.988, 5.023,low dose group B χ2 =5.102,5.234,all P <0.05).Conclusion Porcine pulmonary surfactant(import)and bovine pulmonary surfactant(domestic)full dose early treatment of late preterm children with respiratory distress syn-drome could significantly relieve pulmonary symptoms and signs,effects are better than low dosage,and has high secu-rity,low dosage treatment does not reduce the cost of treatment,bovine pulmonary surfactant therapy administered in an amount sufficient lower cost,select the appropriate treatment plan based on your specific needs.

Objective To evaluate the effect of class 10000 laminar flow ward on the incidence of healthcare-asso-ciated infection (HAI)in patients with initial occurrence of acute leukemia during induction chemotherapy period. Methods Patients with initial occurrence of acute leukemia admitted to a hematological department of a hospital be-tween October 2013 and June 2014 were investigated retrospectively,patients in class 10000 laminar flow ward was as trial group,in general ward was as control group. All patients received standard induction chemotherapy and the same nursing measures,the incidence of HAI between two groups of patients,and ward air cleanliness were com-pared.Results A total of 79 patients with initial acute leukemia were received (trial group,n= 39;control group, n= 40). The average air cleanliness value in rooms and corridors of laminar flow wards were both significantly dif-ferent with general ward (3.57×106/m3 vs 149.36×106/m3 ,t= 45.80,P<0.001;24.46×106/m3 vs 15854.38 ×106/m3 ,t= 108.70,P<0.001). Incidence of HAI between trial group and control group was significantly differ-ent (23.08% [9/39]vs 45.00% [18/40],χ2= 4.219,P= 0.040). The main infection site in trial group was gastro-intestinal tract (n= 5 ),in control group was lower respiratory tract (n= 8 ). The duration of fever,duration and cost of antimicrobial use in trial group were (6.20±2.10)d,(9.35±2.12)d,and (27113.79±1559.03)yuan re-spectively,in control group were (10.20±2.90)d,(14.15±3.14)d,and (58566.29±2217.54)yuan respectively, difference in duration of fever and cost of antimicrobial use between two groups were all significant(t= 1.021, 1377.45,both P<0.05).Conclusion Laminar flow ward can reduce the incidence of HAI in patients with initial occurrence of acute leukemia,and decrease cost of antimicrobial use.

Congresses as Topic ; Humans ; Otorhinolaryngologic Surgical Procedures ; Periodicals as Topic

Metabolomics is one of the branches of systems biology,which employs nuclear magnetic resonance and mass spectrometry technologies to detect the abnormal metabolites from a variety of body fluids. Study of various molecules and their functions may help to find the disease-related early metabolic marker clusters,understand the molecular mechanism of pathogenesis,which provides broad prospects for the early diagnosis and individualized treatment of malignant tumors.

Rational clinical nutrition support may slow down the progress from heart insufficiency to heart failure and improve the quality of life.This article summarizes advances in nutrition support in patients with heart insufficiency in terms of nutrition and metabolic characteristics, nutritional risk screening and nutrition status evaluation, nutrient recommendations, and clinical application of nutrition support.Moreover, nutrition and metabolic support in acute heart failure is also discussed.