Objective To study the effect of inosine monophosphate dehydrogenase inhibitor (IMPDHI) on chemotaxis, migration and endocytosis of human peripheral myeloid dendritic cells (MDCs). Methods Freshly isolated peripheral blood mononuclear cells(PBMC)collected from healthy volunteers (N=15) and the study group were treated with IMPDHI. CC chemokine receptors on MDCs were analyzed by flow cytometry. The study group, control group and different chemokines were added via trans-well approach for different chemokines, stained by Lin-1/CD11c/HLA-DR and counted by flow cytometry. The migration index was calculated as a percentage of MDC migrated in response to the tested chemokine. After isolation of blood dendritic cell antigen-1+ (BDCA-1+ ), mannose receptor-mediated endocytosis was measured as the cellular uptake of FITC-dextran by the flow cytometry. Results (1) Compared to the control group, the expression of CCR1 in the study group was up-regulated significantly(17.02±3.23～30.63±9.13, P＜0.05) and the expressions of CCR3(10.26±2.25～5.81±0.97 P＜0.05) and CCR7 (9.56± 1.84～5.18±0.60 P＜0.05)were downregulated significantly. MDCs in the study group showed enhanced migratory response to inflammatory chemokine CCL2, CCL3, CCL4, CCL7 and CXCL12 (P＜0.05). (2)The endocytosis capacity in the study group was significantly higher than that in control group (P ＜0.05). Conclusion IMPDHI enhances the endocytotic capacity of MDCs and impairs the migratory response of peripheral MDCs to lymphocytic tissue by up-regulating the expression of chemokine receptor in MDCs and enhancing migratory response to inflammatory chemokines.

Objective To study the effect of inosine monophosphate dehydrogenase inhibitor (IMPDHI) on maturation, migration, endocytosis and allostimulatory properties of human peripheral myeloid dendritic cell (MDC). Methods PBMC from healthy donors were isolated. MDC were cocultured with PBMC and exposed to mycophenolic acid (MPA) for 48 h. The expression of co-stimulatory and adhesion molecules as well as chemokine receptors on MDC was analyzed by flow cytometry. In separate experiments,MDC were cultured with or without MPA, and their endocytosis function was estimated by means of FITC dextran uptake. MDC migration experiments were performed in Transwell chambers. Inflammatory chemo kines were added to the lower chambers and MDC numbers were analyzed by flow cytometry. MPA treated (48 h) BDCA-1 + DC served as stimulator cells in MLR. Allogenic healthy CD4 T responder cells were labeled with fluorescent dye CFSE and measured by flow cytometry. Results Maturation: compared to the control group, the expression of CD40, CD62L, HLA-DR, CD54, CD80, CD83 and CD86 on MDC in study group were significantly down-regulated ( P ＜ 0.05 ). Chemokine receptor and migration: compared to control group, the expression of CCR1 on MDC in study group was up-regulated significantly (17.02 ±3.23 vs 30.63 ± 9.13, P ＜ 0.05 ), the expression of CCR3 ( 10.26 ± 2.25 vs 5.81 ± 0.97, P ＜ 0.05 ) and CCR7(9.56 ± 1.84 vs 5.18 ±0.60, P ＜0. 05) on MDC were down-regulated significantly in the study group.MDC in study group showed enchanced migratory response to inflammatory chemokine CCL2, CCL3, CCL4,CCL7, CXCL12 (P＜0.05). Endocytotic capacity: the capacity of endocytosis in study group was signifi cantly higher than that in control group( P ＜ 0.05 ). Llostimulatory capacity: MPA-treated MDC exhibited a markedly reduced ability to stimulate allogenic CD4+ T cell proliferation. Conclusion Treatment of MDC with MPA exhibited an immature phenotype, a propensity to migrate in response to inflammatory chemokines, increased endocytotic capacity and impaired allogenic ability of MDC.

Catecholaminergic polymorphic ventricular tachycardia(CPVT)is a highly fatal inherited arrhythmia induced by emotional stress or exercise.It can be triggered by rapid polymorphism of ventricular tachycardia and ventricular fibrillation, and may lead to syncope or sudden death, with a poor prognosis.Genetic testing is one way to diagnose the disease.It has been found that the disease is related to abnormalities of RyR2, CASQ2, TECRL and other genes, whose mutations affect calcium homeostasis and lead to abnormal electrophysiological activity of the heart, leading to delayed depolar(DADs), and subsequently to malignant arrhythmia.This paper reviewes the mutation of the new pathogenic gene TECRL gene in catecholamine sensitive ventricular tachycardia, through the understanding and learning of the mutation gene reported in the previous literature, in order to further explore the pathogenesis of the disease, learn to deal with the occurrence of malignant arrhythmia, and promote the clinical precise treatment of the disease.

Objective To explore the methods for solving the neck two-point T2-DIXON fat-water separation misalignment. Methods During August 2015 to July 2016, 140 patients with fat-water separation on the nasopharynx and cervical axial T2-DIXON images were prospectively recruited from Sichuan Cancer Hospital. There was no metal implant in the fat-water separation misalignment area. The patients were divided into 7 groups by random number table method with 20 patients in each group for the axial T2-DIXON scan: Group A adopted plan 1( increasing localized shim box on the fat-water separation misalignment area);Group B adopted plan 2(increasing the times of repeated acquisition to 2 of the T2-DIXON sequence);Group C adopted plan 3 (placing the shimming assist device on the inspection area);Group D adopted plan 1+plan 2;Group E adopted plan 1+plan 3;Group F adopted plan 2+plan 3;Group G adopted plan 1+plan 2+plan 3. The images quality of two scans were graded and compared. We compared the signal-to-noise ratio (SNR) of T2-DIXON image on the same muscle tissue between the two scans of each group. The difference of SNR on the two scan images was compared with the paired t test, the difference of SNR among seven groups was conducted with independent sample t test, and the comparison of image quality classification was conducted by rank sum test. Results The image quality of all the seven groups was improved to some different degrees. The cases with image quality reaching level 3 were 12, 15, 15, 16, 17, 18 and 18 in A to G groups, respectively. It was better to use the combination of two or more methods to improve the quality of the image than to use a single method. There were no statistically significant differences in SNR between two scans in A, C and E group (all P>0.05).There were statistically significant differences in SNR between two scans in groups of B, D, F and G(all P<0.05). There were statistically significant differences among the 7 groups, with the best quality in G group (P<0.05). There was no significant difference in the first SNR among the 7 groups (P>0.05);there were significant differences in the second scans among the 7 groups (P<0.05) .Conclusions This study suggests that placing localized shim box, increasing the times of repeated acquisition, and use of shimming assist device in MRI correct the fat-water separation misalignment, help to provide images with high quality. The combination of the above method was better than using the single method. The SNR can be improved when increasing the times of repeated acquisition.