ObjectiveTo observe the effects of free fatty aids (FFAs) on the apoptosis and migration of human breast cancer cells ( MDA-MB-231 ).MethodsBreast cancer cells were exposed to oleic acid (OA) and palmitic acid (PA) with different concentrations (50,200,and 500 umol/L).Quantity RT-PCR was conducted to detect mRNA expression of PTEN,and Western blot to detect protein expression of PTEN and Bcl-2.Cell apoptosis was measured with TUNEL method,and migration measured with transwell method.ResultsThe mRNA and protein expressions of PTEN in MDA-MB-231 cells were down-regulated after OA stimulation,but up-regulated after PA stimulation ( all P ＜ 0.05 ).The protein expression of Bcl-2 increased after OA stimulation and decreased after PA stimulation ( both P ＜ 0.05 ).PA increased the apoptosis,and both OA and PA enhanced the invasion of MDA-MB-231 cells.ConclusionsPA may promote apoptosis of MDA-MB-231 cells through changing the expression of the tumor suppressor PTEN,but also increases the invasion of the cancer cells.The invasion of cancer cells could be enhanced by OA stimulation as well.

Objective To analyze the literature on urinary incontinence after radical prostatecto-my by bibliometric method,and to understand the research status and development trend in this field.Methods The Web of Science core collection database was searched for articles on urinary inconti-nence after radical prostatectomy from January,1 2013 to March,25 2023,and the visual analysis of the annual number of publications,countries,institutions,authors,source journals and keywords was performed by VOS viewer and CiteSpace software.Results A total of 1 212 relevant literatures were retrieved,and the annual number of publications was on the rise.The United States ranked the first in the number of publications,followed by Germany and Italy,among which the United States cooper-ated more with other countries.The institutions ranking top 10 were mostly concentrated in the United States,and the high-contribution institutions were mainly Memorial Sloan-Kettering Cancer Center and University of California,San Francisco.The author with the largest number of articles was MAT-THEW R,and many stable cooperation teams had been formed among the authors.The main journals in this field included Journal of Urology,European Urology,etc.The research hotspots in this field mostly focused on the relationship between influencing factors,evaluation diagnosis,treatment meth-ods and outcome indicators of urinary incontinence after radical prostatectomy.Urinary incontinence rehabilitation,biofeedback,local therapy,robot-assisted radical prostatectomy,and patient reported outcomes have been active topics in recent years.Conclusion Urinary incontinence after radical prostatectomy has attracted more and more attention from scholars.However,compared with the de-veloped countries such as the United States,the total number of papers published in China is less,and the academic influence and exchanges between scholars are weak.In the future,it is advisable to learn from relevant research results of foreign countries,strengthen international scientific research exchange and cooperation,and promote the development of related research on urinary inconti-nenceafter radical prostatectomy in China.

Objective To analyze the literature on urinary incontinence after radical prostatecto-my by bibliometric method,and to understand the research status and development trend in this field.Methods The Web of Science core collection database was searched for articles on urinary inconti-nence after radical prostatectomy from January,1 2013 to March,25 2023,and the visual analysis of the annual number of publications,countries,institutions,authors,source journals and keywords was performed by VOS viewer and CiteSpace software.Results A total of 1 212 relevant literatures were retrieved,and the annual number of publications was on the rise.The United States ranked the first in the number of publications,followed by Germany and Italy,among which the United States cooper-ated more with other countries.The institutions ranking top 10 were mostly concentrated in the United States,and the high-contribution institutions were mainly Memorial Sloan-Kettering Cancer Center and University of California,San Francisco.The author with the largest number of articles was MAT-THEW R,and many stable cooperation teams had been formed among the authors.The main journals in this field included Journal of Urology,European Urology,etc.The research hotspots in this field mostly focused on the relationship between influencing factors,evaluation diagnosis,treatment meth-ods and outcome indicators of urinary incontinence after radical prostatectomy.Urinary incontinence rehabilitation,biofeedback,local therapy,robot-assisted radical prostatectomy,and patient reported outcomes have been active topics in recent years.Conclusion Urinary incontinence after radical prostatectomy has attracted more and more attention from scholars.However,compared with the de-veloped countries such as the United States,the total number of papers published in China is less,and the academic influence and exchanges between scholars are weak.In the future,it is advisable to learn from relevant research results of foreign countries,strengthen international scientific research exchange and cooperation,and promote the development of related research on urinary inconti-nenceafter radical prostatectomy in China.