Objective To study the disease chart among pre-hospital first aid in Tangshan people'hospital.Methods Prospectively collecting data of pre-hospital first aid 2580 patients from 2006 to 2007,analyzing the data,sex,age,visitingtime,emergency diagnosisand results,etc.Results Among pre-hospital first aid 2580 patients,traf-rice diseases,neurosystem diseases,cerebral infarction,coronary heart disease are the first three diseases.The highest times of pre-hospital first aid are 8:00～10:00,14:00～16:00 and 20:00～22:00.The admission rate is 44.46%.Conclusion Pre-hospital first aid should be based on the data,age,visiting time of the disease,scientifically arranging pre-hospital,training for pre-hospital first aid staff,so,the patients will be the fast and best pre-hospital flint aid.

BACKGROUND:Drug treatment is the main method for the treatment of diabetes currently, but the development of the disease and occurrence of related complications are the chal enges to the effect of drugs. OBJECTIVE:To investigate the effect and feasibility of co-transplantation of bone marrow mesenchymal stem cells and islet cells for the treatment of diabetes. METHODS:The rat bone marrow mesenchymal stem cells were separated and purified, and cultured in vitro to establish the diabetes models. The rat diabetes models were injected with bone marrow mesenchymal stem cells, co-cultured mixture of bone marrow mesenchymal stem cells and islet cells, and normal saline or phosphate buffer (control). The effect of transplantation was evaluated through observing the blood glucose levels, insulin secretion, and pathological changes of pancreatic tissue in the rat diabetes models. RESUTLS AND CONCLUSION:In the diabetes rats treated with bone marrow mesenchymal stem cel transplantation, the C-peptide levels were significantly increased after transplantation, while the blood glucose levels were significantly decreased, but not lower than the normal level, and the blood glucose levels were increased again with the time prolonging. In the diabetes rats treated with co-transplantation of bone marrow mesenchymal stem cells and islet cells, the blood glucose levels were decreased significantly and lower than the normal level which was maintained in a certain time, and the decreasing degree was larger than that in the rats treated with simple bone marrow mesenchymal stem cel transplantation. Co-transplantation of bone marrow mesenchymal stem cells and islet cells is feasible for the treatment of diabetes with a certain effect.

Objective To study the relationship between parathyroid hormone(PTH)level and central arterial stiffness in patients with high risk of coronary artery disease(CAD) and preserved renal function.Methods Seventy-seven patients with at least one risk factor of coronary artery disease (CAD) were divided into increased arterial stiffness group(n=35) and control group(n=42),based on whether the noninvasive pulse wave analysis index of the central pulse pressure(CPP) was higher than 40mmHg or not.Coronary artery angiography was performed in all cases.Serum intact PTH,calcium,phosphor,and highly sensitive C reactive protein (hsCRP) was assessed by radioimmunoassay.Coronary angiography was also performed.Results The patients in increased arterial stiffness group were elder and had higher serum uric acid compared with those in the control group.Forty-four patients were confirmed to have CAD; CAD patients in the stiffness group (n=23) had significantly higher serum iPTH level compared with those in the control group(n=21) (P40 mmHg)after adjustment for age,BMI,hs-CRP,and male gender.Conclusion CAD patients with increased central arterial stiffness and preserved renal function have higher serum PTH level.The serum PTH level is positively correlated with central arterial stiffness,and is the independent risk factor for increased arterial stiffness.

Objective In order to understand the depression situation of the hospitalized tuberculosis patients.Methods Zung depression was used as a tool for assessment,the objective of 130 patients hospitalized for tuberculosis survey.Results 58.5% of the hospitalized tuberculosis patients showed symptoms of depression,compared with the secondary and 3 care patients,the rate was high among the level care patients,meaningful differences(P

Objective To study the effects ofShenqi YizhiGranules (SQYZ) on learning and memory and content of Aβ1-42 of cerebral tissue in 5XFAD mice with Alzheimer’s disease; To discuss its mechanism on improving learning and memory ability of 5XFAD mice.Methods Four-month-old C57BL?6 wild type mice were randomly divided into NS control group and SQYZ control group, and the 5XFAD mice were randomly divided into model group, SQYZ group and huperzine-A (HupA) group, 15 mice in each group. Each group were given same volume for gavage for 60 d. After treatment, the learning and memory ability were evaluated by nesting test, passive avoidance and Morris water maze test. The senile plaques and content of Aβ1-42, ionized calcium binding adapter molecule 1 and glial fibrillary acidic protein in cerebral cortex and hippocampus were detected by immunohistochemical staining and immunofluorescence, respectively.Results Compared with NS control group, the score of nesting test in model group significantly decreased; the step-through latency in passive avoidance was shortened and the escape latentcy in Morris water maze test was prolonged; the quantity of senile plaques and content of Aβ1-42 increased in cerebral cortex and hippocampus; the activation of glial cells significantly increased. In the SQYZ group, the above-mentioned indexes reached or approached the level of wild type control mice. The difference between SQYZ group and model group was statistically significant (P<0.05,P<0.01).Conclusion SQYZ improved learning and memory ability in 5XFAD mice, which may be related to reduction of senile plaques, inhibition of over activation in glial cells and reduction of content of Aβ1-42 in cerebral cortex and hippocampus.

Objective To explore the effect of psychological intervention of the patients with acute stroke in pre-hospital first aid. Methods 148 patients with acute stroke who had been treated by psychology in pre-hospital first aid were chosen and interviewed, comparing with 100 patients with acute stroke in hospital in same state. Results There was a significant difference between two groups about realization to disease, attitude to disease, mental reaction, treating confidence, compliance, and so on. Conclusion Psychological intervention can improve the patients with acute stroke in pre-hospital first aid effectly in the realization about disease, correct attitude about disease, capability of fighting diseases, confidence for recovery from the illness and compliance.

Objective To explore the effects of family member's behaviors on outcome of patients with acute cerebral infarction. Methods 160 cases with acute cerebral infarction were investigated with 9 kinds of family member's behavior. Results 9 kinds of family member's behavior were all closely related to the outcome of patients with acute cerebral infarction. Conclusion Family member's behavior was closely related to the patients with acute cerebral infarction

[Objective] To evaluate the diagnostic values of multi-detector spiral computed tomography (MDCT) in pediatric sacrococcygeal tumors (SCT) and to improve the diagnostic ability.[Methods] 54 children (22 male and 32 female,age between 1 day and 16 years old) with pathologically confirmed SCT were involved in our study.All of them received 64-row spiral Computed Tomography before surgery,CT characteristics and clinical data were retrospectively analyzed.[Results] Pediatric SCT are more common in female children under four years old,with the germ cell tumors most common,followed by neurogenic tumors.Among the 54 SCT,39cases were malignant and 15 were benign (malignant∶ benign =2.60∶1).In CT image findings,37 cases (68％) were mainly solid mass,with 31 cases confirmed malignant by pathology.8 cases (15％) were mainly cystic,with all of them confirmed benign by pathology.9 cases (17％) were cystic-solid or with obvious necrosis in solid mass,with 8 cases confirmed malignant by pathology.[Conclusion] Malignant pediatric SCT are more common than benign SCT.Most malignant SCT are mainly solid mass or cystic-solid or with obvious necrosis in solid mass,and most benign tumors are mainly cystic.Combined with clinical data,MDCT can help to correctly diagnose SCT before surgery.

OBJECTIVE:To prepare aspirin phospholipid complex (ASP-PC) and conduct the characterization. METHODS:Using the combination rate of ASP and PC as index,single factor test was used to screen the preparation method of ASP-PC,PC type,solvent type,reaction time,reaction temperature,solvent volume and drug-lipid ratio. The verification test was conducted. UV spectrophotometry,Thermogravimetric analysis,X-ray diffraction and Fourier transform infrared spectroscopy were used for the characterization of ASP-PC. RESULTS:Magnetic stirring-condensing reflux method was adopted,drug-soybean phospholipids ratio was 1:3 (mol/mol),solvent was tetrahydrofuran,reacting for 3 h under 58 ℃. The average combination rate of prepared ASP-PC was 83.52％(RSD=1.16％,n=3). Compared with ASP,physical mixture of ASP and PC,UV spectrum showed that ASP-PC had no new absorption peak. Thermogravimetric analysis,X-ray diffraction and Fourier transform infrared spectroscopy showed the ASP and PC in ASP-PC were interacted;and ASP-PC changed little in quality within 0-300 ℃. CONCLUSIONS:ASP-PC can be successfully prepared,in which,ASP and PC were combined successfully;while there are still trace amounts of ASP in the form of crystals.

Objective To investigate the relationship between the levels of adiponectin and leptin in umbilical bloods and fetal' s growth and development. Methods Eighty-six neonates bom in our obstetrics department from October 2006 to October 2007 were enrolled the study. According to different birth weight they were divided into small for gestational age infants (SGA) group( n = 16), appropriate for gestational age (AGA) group( n = 41) and large for gestational age (LGA) group ( n = 29 ). After the baby was delivered, 20ml umbilical cord blood was collected immediately.Serum adiponectin and leptin levels of umbilical blood were measured using the radio immunoassay, while blood lipid levels were examined and neonatal growth parameters were measured. Results (1)There were significant difference of umbilical blood leptin and adiponectin levels among three groups ( P < 0.001), which were higher in LGA group than those in AGA and SAG group(P <0.01). (2)There were positive correlations between the serum leptin levels and body weight, gestational age, head circumference, body length, foot length and placental weight( P < 0.01). There were positive correlations between the umbilical blood levels of adiponectin and birth weight, head circumference, foot length and BMI(P<0.05).(3)Umbilical blood leptin levels were significantly positively correlated with adiponectin (P <0.01). (4) Blood lipid levels were not found significant difference among three groups, there were no significant correlation between umbilical blood adiponectin and leptin levels and blood lipids ( P > 0.05). Conclusion Umbilical blood leptin and adiponectin play an important role in fetal growth and developmental processes.