This paper is maintenance of four kinds of failures of Carestream GC1.5 the workstation used several years to summarize, workstation software, change the host, burn, workstations transmission, and four kinds of failures of the specific case of itemized elimination steps are introduced.
Computers ; standards ; Maintenance ; Software

Macrovascular complications are the main death or disabling causes of diabetic patients.In recent years,the results of a number of clinical trials aimed at preventing diabetic macrovascular complications have been unveiled.Comprehensive and systemic analysis of these results may give great enlightenment to the clinicans,as well as promote the work in preventing diabetic macroangiopathy in China.

Objective To investigate the expression and clonality of TCR V? subfamily T cell in 11 patients undergoing chronic hemodialysis before and after renal transplantation.Methods The CDR3 of TCR V? 24 subfamily genes were amplified in samples of peripheral blood mononuclear cells which were drawn before hemodialysis and at 20th day post-operatively. To observe the usage of TCR V? repertoire, the RT-PCR products were further labeled with fluorescent and analyzed by genescan technique for CDR3 size.Results (1) One patient was attacked by acute cellular rejection (ACR) and one suffered from delayed graft function (DGF) diagnosed by renal transplant biopsy. (2) 1-10 TCR V? subfamilies T cells could be identified before transplantation, and most of TCR V? subfamily T cells expressed as polyclonality. The most frequent expression of TCR V? genes was V?3. (3) Only 1-5 TCR V? subfamilies T cells could be detected post-operatively, most of them expressed as oligoclonality. The TCR V?3 subfamilies still were the most frequently expressed (in 9 cases). (4) There were no TCR V? subfamilies T cells before pulse of methylprednisolone, and were 5 subfamilies during pulse of methylprednisolone expressed as oligoclonality or biclonality, 2 subfamilies after ACR expressed as polyclonality. In DGF patient, there were 4 TCR V? subfamilies during DGF, and 5 following DGF.Conclusion (1) The significantly skew distribution of TCR V? subfamily T cells could be found in all patients with chronic renal failure and chronic hemodialysis. (2) Furthermore, the skewing distributions of TCR V? genes came to more significant at 20th day post-transplantation than before. (3) ACR might be closely associated with some special clones of TCR V? subfamily T cells, which subsided immediately after ACR. (4) The expression as polyclonality after ACR and DGF may indicate that the immune function partially was inclined to normalization even though immunosuppressed.

Three-dimensional (3D) printing technique has been improving the industrial process from uniform pipeline production procedure in manufacture into individualized production with distributed network. 3D printing technique also provokes these changes in the field of medicine, especially in orthopedics, stomatology and radiology. The role of 3D printing technique has been increasingly highlighted in tumor radiotherapy. Current studies and application mainly focus on personalized tissue compensato (bolus), brachytherapy (high-dose post-loading and particle implantation therapy), 3D printing personalized phantom and individualized fixtures, etc. In this article, research progresses on the application of 3D printing technique in radiotherapy at home and abroad were reviewed.

Objective To study the factors which affect the preparation of compound lobelia gel and establish the optimal preparation process. Methods Based on single factor test, formability, spread performance, stability were used as comprehensive evaluation indicators to select the preparation process by orthogonal design, taking the dosage of carbopol-940, drug loading, 5% hydroxy ethyl benzene ethanol solution and triethanolamine as factors.The content of scutellarin in gel was determined by HPLC.Results The best prescription of compound lobelia gel was as follows: substrat of carbopol-940 was 0.25 g, 10% Azone for promoting the permeability was 2 g, pH regulator of triethanolamine was 1.0 g, moisturizer of glycerol was 0.4 g; preservatives of 5% ethylparaben ethanol solution was 0.3 g; the drug loading was 1 g processed herbs per 1 g gel. Conclusion The preparation process of compound lobelia gel is simple, the product is texture and delicate stable.

Objective To investigate the influence of BK virus (BKV) activation in renal transplant recipients on the renal allograft function.Method Recipients receiving renal transplantation during 2010.3-2011.4 were sdected as objectives,the urine and peripheral blood samples of them were taken and real-time PCR assays were performed to detect BKV DNA at 0.5,1,3,6,9,and 12 months post-transplantation.Results Among 88 recipients,BKV viruria occurred in 27 (30.68％) patients,and sustained viruria occurred in 17 patients.37.0％ (10/27) of patients with BKV viruria developed inot BKV viremia,and sustained viremia occurred in 5 patients.The viral load in plasma was higher in patients with sustained viremia than in those with transient viremia (P＜0.05),and serum creatinine concentrations were higher when BK viremia occurred (P＜0.05).Conclusion Graft function was impaired among patients with BK viremia,and regularly monitoring BK virus in renal transplant recipients and clinical imervention based on plasma PCR results can prevent transplant kidney damage effectively.

Objective To compare the applied value of BK virus DNA load detection in urine and plasma for diagnosis BK virus nephropathy (BKVN) in renal transplantation recipients.Method In 88 renal transplantation recipients receiving renal allograft from February 2011 to January 2012 in our institute,BK virus DNA load in urine and plasma was detected by using real-time PCR,and renal biopsy was performed on the recipients with gradual deterioration of the graft function or the loads of BKV replication being very high.The diagnosis of BKVN was confirmed by using immunohistochemistry.Results Of 88 recipients,there were 35 cases (39.8％) of viruria,18 cases (20.5％) of viremia and 5 cases (5.7％) of BKVN.The median BKV DNA load in both urine and plasma in BKVN recipients was significantly higher than in non-BKVN recipients (P＜0.05).The viruria sensitivity and specificity for BKVN were 100％ and 57.3％ (P =0.03),and the viremia sensitivity and specificity for BKVN was 100％ and 82.9％ (P =0.0002),respectively.We regraded viral load ≧ 105 copies/mL in plasma or ≥107 copies/mL in urine as the best discriminant cut-off value to predict the disease and to identify patients at risk of developing BKVAN.The positive cut-off value of urine's positive predictive value (PPV+) was 26.3％ and negative predictive vaule (PPV-) was 95.7％,and the positive cut-off value of plasma's positive predictive value (PPV +) was 83.3％ and negative predictive vaule (PPV-) was 98.8％.Conclusion The viral load ≥105 copies/mL in plasma can be used as the best discriminant cut-off value to predict the disease and to identify patients at risk of developing BKVAN,but the cut-off value of urine should be only used for screening BKV infection.

Objeetive To investigate the effect of TURP on the quality of life of elderly PCa patients with LUTS symptoms.Methods A retrospective analysis of the clinical data from January 2012 to January 2014 of 75 patients admitted to our hospital after prostate puncture biopsy and pathological diagnosis of PCa in elderly patients,mean age (79 ± 7) years old,which 44 cases were associated with different degrees of LUTS symptoms,of which,20 underwent TURP.After the rule of endocrine therapy,we observe and record of patients before and after treatment and 3,12,6 months after the IPSS and QOL score,compared with the changes in the quality of life of patients.Results Forty-four cases of patients with IPSS score in treatment group after 3,6 and December were (6.25 ± 2.53),(5.15 ± 2.25),(5.00 ± 2.36).Compared with the preoperative IPSS basal value (30.55 ± 3.62),the difference was statistically significant (P ＜0.01).However,there was no significant difference in the postoperative patients (P =0.209,0.863,0.154).The QOL score of treatment group after 3,6 and 12 months were (1.35 ± 1.14),(0.85 ± 0.75) and (0.70 ± 0.87).Compared with the preoperative QOL basal value (4.70 ± 0.73),the difference was statistically significant (P ＜ 0.01).However,there was also no significant difference in the postoperative patients (P =0.078,0.023,0.593).Compared with the control group of IPSS and QOL score,there was no significant difference before treatment in the treatment group (F =0.105,P =0.747),however,there was significant difference after treatment(P ＜ 0.01).Conclusions TURP is a safe and effective method to improve the quality of life and improve the quality of life of patients with prostate cancer.

Objective To study complement receptor typeⅠ(CR1)activity of erythrocytes in pa-tients with SLE.Methods Using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP),CR1rosette(RBC-CR1R)and immuno-complex rosette(RBC-ICR)on red cell,the ery-throcyte complement receptor I type(ECR1)genomic density polymorphism(HH type,HL type,LL type)and erythrocyte CR1immune activity were determined in32patients with SLE and in48normal individuals.Results It was found that HH type rate of ECR1density polymorphism in patients with active SLE was significantly lower(10/16,62.5%)than that(13/16,81.3%)in patients with stable SLE.The level of CR1immune activity in HH type was significantly higher than that in HL,LL type of SLE,and significantly dif-ferent from that in48normal individuals(P

Objective Recurrence of local segmental glomerulosclerosis (FSGS) in 2 patients with renal allograft was reported.Methods A male child aged 15 years and a male adult aged 25 years with primary FSGS, who were subjected to cadaveric kidney transplantation, had a recurrent nephritic syndrome showing massive proteinuria, hyperlipidemia and hypertension respectively in 2 weeks and 18 months postoperatively, that was suspected a recurrent FSGS. The child immediately was treated with Benazepril hydrochloride, 30?mg /day plus high dosage of Prednisolone ( 1?mg /kg every day) for 6 weeks, but proteinuria did not to be ameliorated. The adult was treated with high dosage of Prednisolone ( 1?mg /kg every) and Tripterygium wifordii for 12 weeks, but the syndrome was not improved. Results Two patients had recurrent FSGS according to renal biopsy revealing characterized by similar features: diffuse effacement of foot processes on electron microscope, segmental or focal sclerosis under light microscope and IgM, IgG, C3 deposits. The therapy of plasmapheresis as well as high dosage of Benazepril hydrochloride was added to institute respectively for continuous 3 sessions with removal of 1.5 volume plasma ( 1 200?ml of plasma) in the child and successive 6 sessions with removal of same volume ( 3 000?ml of plasma). The child's proteinuria had a significant reduction from 8.29?g /day to 4.52?g /day after a week post pheresis, that kept 4.52 ～ 5.56 ?g/day with stable creatitine (180～200??mol/L) following 18 months. The adult's proteinaria obvious decreased from 4.68?g /day to 1.50?g /day after plasma exchange a week and keeping decline to 1.06?g /day with normal renal function following 12 months. Conclusion FSGS may immediately be recurrence in pediatric renal transplants. The mechanism of recurrent FSGS may be associated with excessive glomerular filtration, circulating factor altering glomerular permeability, injection of anti thymocyte and lymphocyte immunoglobulin, and hyperlipidemia. Plasmapheresis in combined with ACE inhibitor can reduce proteinuria significantly rather than a single ACE inhibitor.