1.Comparison of Contents of Triterpenoids,Polysaccharides and Heavy Metals in Ganoderma lucidum Sporule Powder and Ganoderma lucidum
China Pharmacy 2007;0(33):-
OBJECTIVE:To compare the contents of triterpenoids,polysaccharides and heavy metals in Ganoderma lucidum sporule powder and Ganoderma lucidum.METHODS:The triterpenoid compounds in Ganoderma lucidum sporule powder and Ganoderma lucidum samples were analyzed by HPLC;five heavy metals in the samples were determined by ICP-MS and the polysaccharides were quantified by anthrone-sulfuric acid method.RESULTS:There were significant differences beween Ganoderma lucidum sporule powder and Ganoderma lucidum in the HPLC characteristic chromatogram of triterpenoid compunds.The total content of polysaccharide in Ganoderma lucidum sporule powder was higher than in Ganoderma lucidum sample.However,the five heavy metals in the two materials showed no marked differences.CONCLUSION:This study provides theoretical basis for the pharmacological difference between Ganoderma lucidum sporule powder and Ganoderma lucidum.
2.Neuroprotective effects of statins in subarachnoid hemorrhage
International Journal of Cerebrovascular Diseases 2015;23(7):554-558
Subarachnoid hemorrhage (SAH) is a common cerebrovascular disease,its disability and mortality rates are higher.It has been confkmed that early brain injury (EBI) and cerebral vasospasm (CVS) are the major pathophysiological mechanisms of causing neurological dysfunction and death after SAH.There are a variety of factors of causing CVS and EBI,including nitric oxide,endothelin,oxyhemoglobin,and proinflarnmatory cytokines.A large number of animal experiments and clinical research have confrmed that statins have neuroprotective effects.This article reviews the neuroprotective effects and its mechanisms of statins in SAH
3.Study on the antioxidant activity of extracts from Enteromorpha prolifera
Chinese Journal of Marine Drugs 2001;0(05):-
Objective To investigate the antioxidant activity of Enteromorpha prolifera by ABTS radical scavenging assay in vitro and provide scientific basis for exploitation of Enteromorpha prolifera.Methods The antioxidant activities of extracts by different solvents from Enteromorpha prolifera and water extracts from different Enteromorpha prolifera samples were investigated by ABTS radical scavenging assay.Moreover,the antioxidant ability of the water extract from Enteromorpha prolifera was evaluated by using caffeic acid and ascorbic acid as positive control standards.Results The water extract from Enteromorpha prolifera provided the highest radical scavenging activity,and the antioxidant capability of 1mL of water extract from Enteromorpha prolifera was equivalent to 1mL of 2.55 10~(-3) mg?mL~(-1) caffeic acid or 1mL of 2.89?10~(-3)mg?mL~(-1) ascorbic acid.However,the extraction methods,dry sample or not and different kinds of Enteromorpha prolifera samples had certain influence on the antioxidant activity of water extract from Enteromorpha prolifera. Conclusion There are abundant antioxidant compounds present in the aqueous extract of Enteromorpha prolifera,and it can be exploited as latent oxidation inhibitor.
4.Proliferation Inhibition and Apoptosis of Human Gastric Cancer Cells Induced by Oridnin
China Pharmacy 2005;0(15):-
OBJECTIVE: To investigate the growth inhibition and apoptosis of BGC-823 gastric cancer cells induced by Oridonin and its mechanism. METHODS: The Oridonin-treated BGC-823 cells were detected using MTT, transmission electron microscopy (TEM), TUNEL and flow cytometry (FCM) etc. RESULTS: Oridonin(16, 32, and 64 ?g?mL-1) significantly inhibited the proliferation of BGC-823 cells in dose and time dependent manner, with the inhibition rates at 68.5%, 90.8%, and 94.7%, respectively within 24, 48, and 72 h after treatment with 64 ?g?mL-1 Oridnin. When treated with 32 ?g?mL-1 Oridonin for 2 h,mitochondria and endoplasmic reticulum were proliferated and swelled under TEM; At 8 h,vacuolization of mitochondria and endoplasmic reticulum were noted, the internal structure disappeared, and the nuclear chromatin margination vacuolization were in typical apoptosis change; At 24 h, BGC-823 cells were arrested at G2/M phase, and the apoptosis rate was 37.8% under FCM. CONCLUSION: Oridonin inhibiting proliferation and apoptosis of BGC-823 cells maybe related to G2/M phase arresting, and Oridonin induce apoptosis of BGC-823 cells may through mitochondria or endoplasmic reticulum apoptosis pathway.
5.Application of peripheral blood gene expression profiling in cancer diagnosis
Journal of International Oncology 2013;40(10):745-748
Peripheral blood gene expression profiling represents the pattern of expression of all genes in peripheral blood cells.By comparing the different gene expression of peripheral blood between cancer patients and healthy control or patients with alternative types of tumors,we can draw genes of different expression and further analyze the characteristic gene expression profiling to assist diagnosis and treatment.There have been remarkable advance in study of peripheral blood gene expression profiling in head and neck tumors,chest tumors,abdominal tumors,urinary tract and male reproductive system tumors,gynecological tumors and so on,which lay a foundation for further clinical applications.
6.Comparative Analysis of Spiral Progress Teaching Method and Traditional Teaching Method in Computer Teaching in TCM Colleges and Universities
Xing ZHAI ; Junhui SHEN ; Guoyong CHEN
Chinese Journal of Information on Traditional Chinese Medicine 2014;(8):121-122
ObjectiveTo analyze the different effects of spiral progress method and traditional method used in computer teaching in TCM colleges and universities.MethodsStudents were divided into the groups of spiral and traditional. At the end of the course, students would have a course examination.Results Students in spiral group got better scores than students in traditional group (P<0.05).Conclusion Using spiral progress method in computer teaching in TCM colleges and universities can help students develop the right thinking model and scientific learning method. It can also improve students’ ability to use computer knowledge in TCM study and research.
7.Investigation of the Use of Antibiotics in Three Two-Level Hospitals in Urumqi City in 2006
Junhui CHEN ; Xiaoyue JIN ; Erjiang WUMAI ;
Chinese Journal of Pharmacoepidemiology 2006;0(04):-
Objective:To investigate the state of the use of antibacterial drugs in three two-level hospitals in Uru- mqi city.Method:According to information provided by the system,the statistics of antibacterial drugs amount purchased in three Urumqi hospitals,DDDs and daily cost were retrospectively analyzed.Result:The amount of the antibacterial pur- chases decreased compared with that in 2005.The antibiotics accounted for 16.14 in the Construction Hospital,25.68 in the Children's Hospital and 33.99 in the Coal Hospital.Cephalosporins accounted for more than 30% of the amount of the purchase of antibacterial drugs in every hospital.Conclusion:The application of antibacterial drug proportion was basically reasonable.Cephalosporins were still the most widely used antibacterial drugs.Through further standardization of clinical application of antibacterial drugs,their share will continue to decline hut the proportion of the use of antimicrobials impor- ted and produced in joint ventures will grow.
8.Anesthesia Plan in Radiofrequency Ablation of Lung Cancer
Sanqiang DING ; Shaobin WANG ; Junhui CHEN
Journal of Chinese Physician 2001;0(01):-
Objective To evaluate the anesthesia efficacy and safety of superior thoracic epidural block combined with proper sedation in patients with radiofrequency ablation of lung cancer. Methods Sixty patients undergoing radiofrequency ablation of lung cancer were performed under epidural block with local anesthetic of 0.8~1.0% lidocaine, and the punctured point was applied at the T 6-7 or T 5-6 . Innovar and propofol were given to the patients during operation if necessary. ECG, HR, BP and SpO 2 were monitored, and anesthesia complications were recorded. Results The anesthesia efficacy was excellent, the intraopetative HR and BP were lower than those of preoperation(P
9.Determination and clinical significance analysis of ANF、PRA、AT-Ⅱ and ALD in the heart failure patients
Junhui LIANG ; Fengqin CHEN ; Fang ZHOU
Chinese Journal of Immunology 2000;0(08):-
Objective:To investigate the effect of ANF, PRA, AT-? and ALD on the occurrence of Heart failure patients and Congestive heart failure(CHF) .Methods:The plasma were measured by radioimmunoassay(RIA) in 163 patients(112 on heart failure patients,51 congestive heart failure)before and after taking Angiotensiin converting enzyme. The results were as foron: ll. Results:The concentrations of ANF, PRA,AT-? and ALD in acute myocardid infarction, Rheumatic valvuar disease, cor pulmonale and Hypertension patients were higher significantly than the contorl group(heart failure)( P 0.05) .After taking ACE inhibitor,AT an extent, the values were decreased-ANF 32.5%, AT-? 25.3 % and ALD 27.1 % , A positive correlation was shown between ANF system and RSSA system. Conclusion: The results suggested that monitoring of ANF,PRA,AT-? and AID with RIA technique showed a beneficial to heart's disease and heart's dysfunction.
10.Curative effect of transcatheter uterine artery embolization in the treatment of fibromyoma(a report of 21 cases)
Junhui CHEN ; Dawu HU ; Tianhong DUAN
Chinese Journal of Radiology 2001;0(05):-
Objective To evaluate the clinical effect of transcatheter ethanol embolization of bilateral uterine artery as a treatment for fibromyoma. Methods Twenty one women with uterine fibromyoma underwent femoral artery puncture and transcatheter uterine artery embolization. Free flow bilateral artery embolization with ethanol were performed on 16 cases, three women undergoing unilateral embolization, and gelatin sponge were used in 2 cases. Results All cases were observed with a follow up of 3 to 64 months (mean 25 months) after the treatment. Nineteen women infused with ethanol were reported noticeable symptomatic improvement, including fifteen women with complete resolution of symptoms. Two women embolized with sponge exhibited clinical response at 2 and 5 months after the procedure, respectively. The marked diminution of tumor size (40%-90%) was sonographically demonstrated. No severe complications were discovered in all 21 cases. Conclusion Uterine artery embolization represented a promising new method of treating fibroids. It had safe and good clinical results. Further investigation would be required to assess its long term effect.