Objective To observe the effect of genistein,octylphenol,and their combination on proliferation of MCF-7 cells and explore the molecular mechanisms.Methods The MCF-7 cells were divided into four groups: control,5?10~(-5)mol/L genistein treated,8?10~(-6)mol/L octylphenol treated,genistein and octylphenol treated.The cell proliferation,cell cycle,phospho-L-tyrosine protein(PTPr),ER?,ER? mRNA and AIB1 mRNA expression of MCF-7 cells was observed by MTT test,flow cytometry,immunocytochemistry and RT-PCR.Results When MCF-7 cells were treated with 8?10~(-6) mol/L octylphenol or 5?10~(-5) mol/L genistein,or both for 72 h,the proliferation ratio was 12.98%,46.16% and 36.44% respectively;the percentage of MCF-7 cells at G_(2)/M were 12.98%,46.16% and 36.44% respectively;the apoptosis ratio of MCF-7 cells were 3.57%,11.41% and 8.24% respectively;the expression of PTPr was(62.84?9.80),(26.75?5.09),(39.15?7.83) respectively.Octylphenol increased the expression of AIB1 mRNA,but genistein and its combination with octylphenol inhibited the expression of ER? and decreased the expression of AIB1 mRNA in nucleus.Conclusion Octylphenol can promote the proliferation of MCF-7 cells,while genistein and its combination with octylphenol inhibit,which mechanism may be related to regulation of PTPr,AIB1 and ER expression.

Objective: To study the effects of octylpheno(lOP)and genistein(GEN)on the expressions of estrogen receptors(ER),amplified in breast cancer 1(AIB1) and proliferating cell nuclear antigen(PCNA) in rat breast cancer.Method:Female SD rats were randomly divided into control,model,GEN treated,OP treated,GEN and OP combined treated groups.RT-PCR and immunohistochemical methods were used to detect the expressions of AIB1,ER,PCNA on normal mammary gland and mammary cancer.Results: Compared with control mammary gland,AIB1 mRNA,ER mRNA,PCNA and ER expressions were up-regulated in mammary cancer.Compared with mammary cancer in model group,the level of AIB1mRNA,ER mRNA and ER expressions were significantly decreased in GEN treated group,while they were significantly increased in OP treated group and PCNA expression was significantly increased too.Compared with OP treated group,the level of AIB1mRNA,ER mRNA,ER and PCNA expressions were partly decreased in GEN and OP combined treated group.Conclusion: Octylphenol can up-regulate the levels of AIB1,ER and PCNA expressions in mammary cancer and may increase the incidence of 7,12-dinmethylbenz[a]anthracene(DMBA)-induced mammary cancer.Genistin or genistein combined with octylphenol can downregulate the levels of AIB1,ER and PCNA expressions in mammary cancer,and inhibit theiincidences.

This paper designs a new distributing structure of nozzles in medical micro-jet device on the bases of emulating results. This new structure can improve the output coefficient and flux of drug delivery. Then, a micro-jet device is fabricated by MEMS technology based on this structure. The experiment results indicate that the number of output droplet is approximated to normal distribution on droplet diameter and velocity. Moreover, the droplet velocity and the flux are both improved in this new micro-jet device, whose values are 2.93m/s and 11.77?l/s respectively.

Objective To investigate whether tumor necrosis factor-? (TNF-?) enhance the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9(MMP-9) in hepatic cancer cell line HepG2 or not. Methods Cultured HepG2 cells were treated by TNF-? with various concentration and time. The morphological changes of HepG2 cells were studied microscopically and the proliferation of HepG2 were detected by methyl thiazolyl tetrazolium (MTT). The expression of VEGF and MMP-9 mRNA in cultured HepG2 were determined by relative quantitative reverse transcription polymerase chain reaction. The VEGF and MMP-9 protein level in supernatants and in cytoplasm were determined by enzyme-linked immunosorbent assay (ELISA) and by immunocytochemical staining, respectively.Results There was a little morphological changes in HepG2 with TNF-? treatment, but no change of cell proliferation in corresponding time. The expression of VEGF and MMP-9 mRNA was enhanced gradually with the TNF-? concentration increasing, the VEGF and MMP-9 protein level in supernatants and in cytoplasm was elevated gradually with the concentration increasing. There was a dependance on the concentration when the concentration of TNF-? was lower than or equal to 10~4 U/L. Furthermore, the effect of promotion was close to peak when the TNF-? concentration up to 10~4 U/L; but no time-effect pattern observed. Conclusion TNF-? can enhance the expression of VEGF and MMP-9 at the level of mRNA and protein in hepatic cancer cell line.

Objective To compare the effect and safety between lightwand and general-laryngo-scope application of trachea cannula in cervical spine fracture patients undergoing general anesthesia. Methods Forty cervical spine fracture patients with tracheal intubation anesthesia were selected,including twenty-eight males and twelve females,aged 18-65 years,ASA physical statusⅡor Ⅲ.These patients were randomly divided into two groups,twenty cases in each group.After anesthesia induction,group L was giv-en general laryngoscope intubation,whereas group C was taken lightwand guided tracheal intubation.The success case of intubation at first,intubation time,adverse reaction of intubation,changes of MAP and HR at intubation and 5 minutes after intubation were observed.Results Compared with group L,success rate of intubation at first was higher (90% vs.60%),intubation time was shorter [(31.3±11.1)s vs.(46.6 ±15.2)s]in group C (P <0.05).The changes of MAP and HR were more stable during intubation in group C compared with group L.Four cases of arrhythmia (20%)occurred in group C,while none in group L.Conclusion Compared with intubation by general laryngoscope,application of tracheal intubation led by lightwand in cervical fracture patients is more effective and safer.

Objective To study the extraintestinal lesions induced by rotavirus(RV)infection and explore the pathogenesis.Methods Simian rotavirus SA11 was cultured in MA-104 cells.After inoculation,the pathological changes in brain,lung,heart,liver,pancreas and kidney tissues were observed,the RV antigens detected,and the apoptotic cells observed.Besides,we stained the filamentous actin(F-actin)with Phalloidine-TRITC,and then quantified the F-actin amount.Results Several pathological changes,inclusive of myocardial interstitial edema,granular degeneration in cardiacytes,hepatic congestion,and hepatocellular vacuolar degeneration,were found,but not found in the brain,lung,and pancreas tissues.Meanwhile,several ultrastructural changes,inclusive of dissolved myocardial F-actin,extended smooth endoplasmic reticulum,swollen mitochondria,and widened perinuclear space,were found.No difference was found in the quantity of myocardial F-actin.Apoptosis was found in liver cells,but not in myocardial cells.RV RNA was detected in tissues such as brain,lung,heart,liver and pancreas tissues.Conclusion All the results suggest that RV may spread from the intestine to various extraintestinal organs and hence induce injury.Filamentous actin depolymerization,cytoskeleton damage and apoptosis induced by RV infection are the important mechanism not only in intestinal damages,but also in extraintestinal lesions.

Objective: To study the effects of zinc on transport function of erythrocyte membrane. Methods: This study was conducted both in vivo and in vitro. In vivo, weanling rats were divided into three groups and fed with different zinc diets (2.2, 28 and 128 mg Zn/kg diet) for four weeks, the transport function of Na +/K + pump, COTS-1, COTS-2, Gardos and RF channels were determined. In vitro, different concentration of zinc (0,5,10,50,100 and 500 ?mol Zn 2+ /L) were added into fresh human blood and the activities of the five transport channels were detected. Results: Proper zinc could keep the highest activities of Na +/K + pump, COTS-2 and Gardos channel. Too low or too high zinc decreased the transport function of these three channels and the activities of COTS-1 and RF channel were increased with the increase of zinc concentration, indicating the competitive function of these two channels. Conclusion: Zinc plays an important role in maintaining the transport function of erythrocyte membrane.

Objective: To examine the effects of the education with glycemic index(GI)on dietary knowledge,attitude and practice of diabetic patients,and on their blood glucose and lipid.Methods: Seventy-two subjects with type 2 diabetes were randomly assingned into two groups. The test group (group GI) mainly learned the knowledge about GI of food. The controlled group (group FEL) mainly learned the knowledge about food exchange list(FEL). Outcomes were assessed by the change of dietary knowledge, attitude and practice, fasting blood glucose (FBG), 2 h postprandial blood glucose ( 2 hPBG), glycosylated hemoglobin (HbA 1c), and blood lipids which include total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), triglycerides (TG) and lipid comprehensive index (LCI). The period lasted five months. Results: The percent of correct answer for GI questions was increased significantly from 0 % before education to 92.2% after education (P

Objective To study the clinical value of three-dimensional vascular reconstruction of power Doppler imaging in monitering acute rejection of renal transplant.Methods Eighty-eight renal allograft patients were studied by three-dimensional vascular reconstruction and graded the vascular flow.Results The ratio of successful renal transplant three-dimensional vascular reconstruction reached 95.5% in 88 cases.Blood flow gradation was 4-5 levels in 30 controls and 0-4 levels in 58 patients with acute renal transplant rejection. Blood flow gradation was closely relevant to the time when the blood flow began to improve and the renal function began to restore after treatment, value of r being -0.9224 and -0.9223 respectively (P

BACKGROUND: Glycemic index (GI) is a physiological index of carbohydrate food. It has been found out in epidemiological studies that GI is useful in controlling and preventing some chronic diseases such as diabetes,cardiovascular disease,obesity.OBJECTIVE:To investigate the acceptance and practical significance of GI knowledge in diabetic patients by comparing with the traditional education with food exchange list (FEL)as the main material.DESIGN:A comparative observational test.SETTING:Institute of Nutrition and Food Safety,Chinese Center for Disease Control and Prevention.PARTICIPANTS: Seventy-two diabetic patients volunteered to join in the study were selected from the Department Endocrinology of Beijiog No.6 Hospital from October 2000 to February 2002,including 36 males and 36 female of 10-70 years old with an average age of 58 years.METHODS:The 72 diabetic patients were randomly assigned to two groups:① GI group which received GI-based nutrition education;② FEL group receiving FEL-based nutrition education.They were educated for 5 months by means of classes,personal education and consultation by telephone.The changes of knowledge,food selection and blood glucose in the diabetic patients were observed before and after education.MAIN OUTCOME MEASURES:Changes of the awareness of nutrition education,fasting blood glucose (FBG) and 2-hour postprandial blood glucose (2hPBG) before and after education.RESULTS: All the 72 diabetic patients were involved in the analysis of results.①The correct answering rates after education were obviously higher than that before education (P<0.01).Before education,the correct rates of answers to GI questions in the Gl group were lower than the correct rates of answers to FEL questions in the FEL group (0 vs.6.5%,P<0.01).After education,the correct rates in the Gl group were higher than those in the FEL group (92.2%VS.79.4%,P<0.01): whereas there were no significant differences in the average correct rate of answer to the same question between the two groups (P>0.05).The Gl group was better than the FEL group in selecting cereal,fruit,bean and its.products (P<0.01).② The FBG and 2hPBG after education were lower than those before education in both groups(P<0.01),whereas there was no significant difference between the two groups (P>0.05).CONCLUSION:The knowledge of GI was better accepted than that of FEL by the diabetic patients in dietary treatment.