OBJECTIVE:To establish the quality criteria for Fufangzhixueling granules.METHODS:TLC was employed to identify Radix Astragali,Rhizoma cimicifuae and Radix Paeoniae Alba,while the content of AstragalosideⅣin Fufangzhix-ueling granules was deteminde by HPLC.RESULTS:TLC findings of Radix Astragali,Rhizoma cimicifuae and Radix Paeoniae Alba showed the spots of same colors as those developed in the corresponding places of control medical materials.The sample size of AstragalosideⅣshowed a good linear relationship within the range of1.551?g～9.306?g(r=0.9996)with the average recovery of97.98%(RSD=1.77%).CONCLUSION:The method is simple,accurate,stable,reliable and with good repro-ducibility,which can be used for the quality control of Fufangzhixueling granules.

OBJECTIVE:To improve the quality standard of rongshuanwan.METHODS:Danshen root,flos,angelica,Chuanxiong in rongshuanwan were respectively identified qualitatively by TLC.RESULTS:The chromatographic spots of samples showed the same colors with those of control agents.The color spectrum was clear without interference of impurity nearby.CONCLUSION:The established method has high specificity and can better control the quality of this drug.

Objective To analyze the influence of early active nursing on muscle strength in patients after major operation in intensive care unit(ICU). Methods Of 96 ICU patients after major operation were enrolled in the study, 49 patients were assigned to routine ICU nursing (control group, excluding 1 case), and 47 patients were assigned to early active nursing (intervention group, excluding 2 cases) by random number table method. The health condition, muscle strength between 2 groups were compared. Results Acute and chronic physiology score after intervention was (10.62 ±2.04) points in intervention group, and (12.54±2.28) points in control group, and there was significant difference, t=4.269 5, P<0.01. The score of muscle strength was (56.93±4.32) points in intervention group, and (49.42±5.48) points in control group, and there was significant difference, t=7.307 1, P<0.01. The incidence of ICU-acquired muscle weakness in intervention group (6.67%, 3/45) was significantly lower than that of control group (43.75%,21/48), χ2=16.681 5, P<0.01. Conclusions Early active nursing is of great importance to improve muscle strength and decrease the incidence of ICU-acquired muscle weakness.

With the rapid development in science and technology,more and more advanced medical devices have been used in clinical practice.Consequently,the maintenance and management of the devices has become a task of modern hospitals.In this paper,the author discusses some issues for their maintenance and management.

Objective To investigate the early endotracheal intubation line to attract the effect of meconium aspiration. Methods April 2005 to April 2007 the relevant cases (A group) and April 2007 to April 2009 the related cases ( B group) were compared. Results B group and A group efficacy was superior (P < 0.05). Conclusion Early tracheal intubation attract ideal effect of meconium aspiration, it is worth promoting.

Objective To correct the nonlinear error of sensor output, a new approach to sensor inverse modeling based on Back-Propagation Fuzzy Logical System (BP FS) is presented. Methods The BP FS is a computationally efficient nonlinear universal approximator, which is capable of implementing complex nonlinear mapping from its input pattern space to the output with fast convergence speed. Results The neuro-fuzzy hybrid system, i.e. BP FS, is then applied to construct nonlinear inverse model of pressure sensor. The experimental results show that the proposed inverse modeling method automatically compensates the associated nonlinear error in pressure estimation, and thus the performance of pressure sensor is significantly improved. Conclusion The proposed method can be widely used in nonlinearity correction of various kinds of sensors to compensate the effects of nonlinearity and temperature on sensor output.

Objective To investigate effect of Splenic Aminopeptide Oral Lyophilized Powder on urinary retinol-binding protein and CRP in children of mycoplasma pneumonia infection with asthma .Methods 60 cases were randomly divided into 2 groups, 30 cases of control group were given conventional anti-inflammatory treatment, azithromycin.30 patients of Experimental group were treated with Spleen Aminopeptide Oral Lyophilized Powder treatment, two groups were treated for 7 days.30 cases of healthy children were selected, given no any treatment.After treatment, urinary retinol binding protein, blood urea nitrogen, creatinine, cough, wheezing symptom recovery, C-reactive protein, blood and other changes were compared between two groups.ResuIts Two groups of children with urinary retinol binding protein, urea nitrogen, creatinine levels were improved in different degrees, and in experimental group, urinary retinol binding protein, urea nitrogen and creatinine decreased significantly compared with control group (P<0.05).Two groups of children with asthma and wheeze improved in different degrees, and cough wheeze, cough and wheeze recovery time of experimental group had more improvement than those of control group ( P<0.05 ) .Two group of C reactive protein and white blood cell level both improved in different degrees, and the experimental group of C reactive protein and white blood cell levels were significantly lower than those of control group (P<0.05).ConcIusion Spleen Aminopeptide Oral Lyophilized Powder can significantly improve the infection status of children with mycoplasma pneumonia, and relieve cough and wheeze symptoms, lower urinary retinol binding protein and C reactive protein level, improve kidney function damage induced by hypoxia.

Objective Through oral vaccination activity on polio,the epidemiological and virological surveillance of acute flaccid paralysis (AFP) case finds its way to develop, so that the realization of eradication on polio may be verified.Method Various kinds of immunization activities such as regular basic immunization, booster vaccination, intensified vaccination and mopping-up operation were adopted among children of the right age in the whole province. Zero case report and active surveillance on AFP were carried out to those population under 15 who had had consultation in medical unit above county level. Each reported case or found case was treated by individual case investigation, follow-up study and sample collection. All the samples were virus-isolated ad identified by RD, Hep-2 and L20B cells. Result Since 1991, an AFP case surveillance system has been built up. Up to the end of 2000, a total of 1,749 AFP cases had been reported in hunan province, from which 1,404 cases samples were collected, and another 218 cases samples of contacts were also collected. A sum of 7 polio wild virus strains was examined out. Since July 1993, no wild strain has been found (isolated). Conclusion Hunan Province has successfully intervened in the circus (spread) of polio wild virus, and hence realized the goal of polio eradication.

Objective To investigate the effect of activation of protein kinase B(PKB) and Caspase-9 signal transduction pathway of human gastric cancer cells on the cell growth and chemosensitivity to etoposide.Methods The gastric cancer cells SGC7901 were treated with etoposide or etoposide plus PKB inhibitor Wortmannin at different time.The growth rates of gastric cancer cells SGC7901 and their sensitivity to etoposide were examined by 3-(4,5-dimethylthiazol-2,1)-2,5 diphanytetrazolium(bromide) assay.Apoptosis of gastric cancer cells was(detected) by flow cytometry.PKB activity was measured by(immunoprecipitation.) Caspase-3 expression and Caspase-9 activity were determined by Western bolt analysis.Results Etoposide induced apoptosis of SGC7901 cells and inhibited its survival effectively,which was much(weaker) 12 h after treatment.PKB(activity) became higher gradually,and Caspase-3 expression,Caspase-9(activity) significantly reduced at 12 h treated with etoposide.(Conversely),after pretreated with Wortmannin,PKB activity remarkably(reduced,) and Caspase-3(expression),Caspase-9 activity markedly increased.(Wortmannin) suppressed growth and potentiated (apoptosis) caused by etoposide.Potentiation of apoptosis by Wortmannin(correlated) with etoposide-induced PKB and Caspase-9 phosphorylation.Conclusions PKB and Caspase-9 signal transduction pathway promotes(human) gastric cancer cells survival and resistance to(chemotherapy.) PKB inhibitor can enhance sensitivity of gastric cancer cells to chemotherapy.

Objective: Through the hospital fixed assets management mode and method of transformation, to play the biggest economic benefit and use efficiency of fixed assets, better service for the hospital development. Methods: Through the bar code and PDA, realize the accurate inventory of fixed assets, improve the efficiency of inventory, and implement the whole bar code tracking and outbound, department transfers of assets in the whole process supervision. Results: It will use department, management department, finance department of equipment management work has focused on an information system platform. Conclusion:It can complete hospital various aspects of fixed assets management, truly achieve account, card, content is consistent.