1.Study on survivin mRNA and protein of BEP-2D cells during its malignant transformation by cigarette smoke condensate
Journal of Chongqing Medical University 1987;0(01):-
Objective:To study the change of the survivin mRNA and protein of BEP-2D cells during its malignant transformation.Methods:Normal BEP-2D cell and BEP-2D cells treated by cigarette smoke condensate(CSC)for 15 weeks(P-15),25 weeks(P-25)and 38 weeks(P-38)were respectively chosen to study the survivin gene and protein by RT-PCR(retro-translation PCR,RT-PCR)and immunohistochemical method.Results:The survivin mRNA was found in BEP-2D cells of P-15,P-25 and P-38,respectively with 0.56,0.80,and 0.81,but not in the normal BEP-2D cell.The survivin protein was found in the normal BEP-2D cell,but not in BEP-2D cells of P-15,P-25 and P-38.The levels of survivin protein expressian were different between BEP-2D cells of P-15,P-25 and P-38 and normal BEP-2D cell,with significant difference between P-15 BEP-2D cell and normal BEP-2D cell(P
2.The Value of X-ray Diagnosis Myodystrophy Leading to Osteoarticular Change(A Reports of 3 Cases and a Review of the Literature)
Journal of Practical Radiology 2001;0(09):-
Objective To study the value of clinical application for X-ray diagnosis in myodystrophy followed by osteoarticular change such as scapula,etc. Methods Different osteoarticular position,size,structure were observed on X-ray films.X-ray features of dysostosis,malformation and dislocation of joint caused by myodsytrophy were analysed in three cases.Results X-ray findings of myodsytrophy in 3 cases included:revolving and displacement of scapula,scapula getting shorterand smaller,dislocation of acromioc cleido joint and shoulder joint and bending and malformation of shoulder joint.Conclusion It can be certain that myodsytrophy directly causes the maltormation of osteoartthritis to make a diagnosis for one who suffers from a myodystrophy with obvious osteodysplasty by X-ray and find out the harm of this kind of disease.Finally we can predict how it goes in anaphase.
3.Retrospective analysis of the effect of gestational hypothyroxinemia on pregnancy outcomes
Junhao XIE ; Yuhuan LIU ; Qin HUANG
Medical Journal of Chinese People's Liberation Army 2017;42(3):211-216
Objective To discuss the influence of gestational hypothyroxinemia to the pregnancy outcomes and fetus development,and find the evidence of hormone replacement therapy.Methods The clinical data of 1141 gravida admitted from Nov.2014 to Oct.2015 were retrospectively analyzed,including the data of systematic antenatal examination,all the data of pregnancy,the materials of delivery,the last ultrasound examination,production status and the thyroid stimulating hormone (TSH) of the newborn etc.,to find the difference of related index.Results Of the 1141 gravida with integral data,200 had past history of thyroid disease,189 showed below normal of free thyroxine (FT4) and 752 were normal ones.The 189 gravida with normal TSH but lower FT4 were divided into group A (0-5% lower than the normal FT4 value,n=60),group B (5%-10% lower than the normal FT4 value,n=40) and group C (10% and above lower than the normal FT4 value,n=89).The ones with both normal TSH and FT4 value served as control group.Compared to the control group,the higher premature delivery rate,incidence of gestational diabetes mellitus and cesarean delivery rate (P<0.05) were found in group C,and more gravida in group B had a history of hypertension and dyslipidemia during pregnancy (P<0.05).The cesarean delivery rate of group B and C were higher than group A.Meanwhile,the rate of group B was higher than control group (P>0.05).At delivery,the maternal weight,BMI,diastolic pressure,and head circumference of fetus in the last ultrasound examination were higher in group C than in control group (P<0.01),but the gestational weeks of the newborn were shorter in group C (38.55 ± 1.86 weeks) than in control group (39.14 ± 1.57 weeks,P<0.01).The 189 gravida with lower FT4 were divided into two groups according to the thyroid peroxidase antibody (TPOAb) level.The head circumference of fetus in the last ultrasound examination was higher in TPOAb(+) group than in TPOAb(-) group (45.99 ± 62.36cm vs.33.23 ± 2.08cm,P<0.01).Conclusions The influence of gestational hypothyroxinemia to pregnancy outcomes and fetus development cannot be ignored,especially for the pregnant women with lower FT4 value (10% and above lower than the normal) or with positive TPOAb.It is suggested to take the thyroid function test in the early stage of pregnancy for those pregnant women mentioned above.
4.Exploration and Practice to Enhance the Quality of Graduation Practice in Pharmaceutical Education
Junhao JIANG ; Qingeng LI ; Ying CHEN ; Baogang XIE
Chinese Journal of Medical Education Research 2006;0(08):-
Under the guidance of the principle of combining practice with employment,we make the exploration to enhance the quality of graduation practice in pharmaceutical education by establishing the corresponding system as the basis,mobilizing students to practice actively as the gurantee,and keeping effective management of papers as the strategy means.
5.Reflections on Community Hospital Pharmacists’Social Responsibilities
Ying CHEN ; Junhao JIANG ; Baogang XIE ; Qingeng LI
China Pharmacy 2001;0(11):-
OBJECTIVE:To study the development of community pharmaceutical care.METHODS:The significance of community pharmaceutical care was elaborated based on its status quo analysis,and the way to improve the pharmaceutical care was discussed theoretically.RESULTS&CONCLUSIONS:Patient-centered community pharmaceutical care is the primary responsibility of community pharmacists,the community pharmacists should strengthen their study,update their knowledge structure and play an important role in community pharmaceutical care.
6.Genetic Variation of the VP1 Gene of the Virulent Duck Hepatitis A Virus Type 1 (DHAV-1) Isolates in Shandong Province of China
Jiming GAO ; Junhao CHEN ; Xingkui SI ; Zhijing XIE ; Yanli ZHU ; Xingxiao ZHANG ; Shujing WANG ; Shijin JIANG
Virologica Sinica 2012;27(4):248-253
To investigate the relationship of the variation of virulence and the external capsid proteins of the pandemic duck hepatitis A virus type 1(DHAV-1) isolates,the virulence,cross neutralization assays and the complete sequence of the virion protein 1(VP1) gene of nine virulent DHAV-1 strains,which were isolated from infected ducklings with clinical symptoms in Shandong province of China in 2007-2008,were tested.The fifth generation duck embryo allantoic liquids of the 9 isolates were tested on 12-day-old duck embryos and on 7-day-old ducklings for the median embryonal lethal doses(ELD50s) and the median lethal doses(LD50s),respectively.The results showed that the ELD5s of embryonic duck eggs of the 9 DHAV-1 isolates were between 1.9 × 106/mL to 1.44 × 107/mL,while the LD50s were 2.39 × 105/mL to 6.15 × 106/mL.Cross-neutralization tests revealed that the 9 DHAV-1 isolates were completely neutralized by the standard serum and the hyperimmune sera against the 9 DHAV-1 isolates,respectively.Compared with other virulent,moderate virulent,attenuated vaccine and mild strains,the VP1 genes of the 9 strains shared 89.8%-99.7% similarity at the nucleotide level and 92.4%-99.6% at amino acid level with other DHAV-1 strains.There were three hypervariable regions at the C-terminus(as 158-160,180-193 and 205-219) and other variable points in VPI protein,but which didn't cause virulence of DHAV-1 change.
7.A novel mutation of GLI3 gene underlying synpolydactyly in a family.
Ranran KANG ; ; Sexin HUANG ; Jie LI ; Yang ZOU ; Peiwen XU ; Ming GAO ; Lijuan WANG ; Hongqiang XIE ; Junhao YAN ; Yuan GAO
Chinese Journal of Medical Genetics 2017;34(4):490-493
OBJECTIVETo detect mutation of GLI3 gene in a family affected with autosomal dominant synpolydactyly.
METHODSGenomic DNA was extracted from peripheral blood samples from members of the family and 100 unrelated healthy controls. Potential mutation was screened by next-generation sequencing and confirmed by Sanger sequencing.
RESULTSA heterozygous frameshift mutation c.480dupC was identified in the GLI3 gene among all patients from the family. The same mutation was not found in unaffected family members and the 100 healthy controls.
CONCLUSIONThe c.480dupC of the GLI3 gene probably underlies the synpolydactyly in this family.
Adolescent ; Adult ; Amino Acid Sequence ; Female ; Humans ; Male ; Middle Aged ; Mutation ; genetics ; Nerve Tissue Proteins ; genetics ; Pedigree ; Syndactyly ; genetics ; Zinc Finger Protein Gli3 ; genetics
8.Application of droplet digital PCR for non-invasive prenatal diagnosis of single gene disease in two families.
Peiwen XU ; Yang ZOU ; Jie LI ; Sexin HUANG ; Ming GAO ; Ranran KANG ; Hongqiang XIE ; Lijuan WANG ; Junhao YAN ; Yuan GAO
Chinese Journal of Medical Genetics 2018;35(2):224-227
OBJECTIVETo assess the value of droplet digital PCR (ddPCR) for non-invasive prenatal diagnosis of single gene disease in two families.
METHODSPaternal mutation in cell-free DNA derived from the maternal blood and amniotic fluid DNA was detected by ddPCR. Suspected mutation in the amniotic fluid DNA was verified with Sanger sequencing.
RESULTSThe result of ddPCR and Sanger sequencing indicated that the fetuses have carried pathogenic mutations from the paternal side in both families.
CONCLUSIONDroplet digital PCR can accurately detect paternal mutation carried by the fetus, and it is sensitive and reliable for analyzing trace samples. This method may be applied for the diagnosis of single gene diseases caused by paternal mutation using peripheral blood sample derived from the mother.
Fathers ; Female ; Genetic Diseases, Inborn ; diagnosis ; Humans ; Male ; Maternal Serum Screening Tests ; Mutation ; Polymerase Chain Reaction ; methods ; Prenatal Diagnosis ; methods ; Sequence Analysis, DNA
9. Exploration on the training model of professional postgraduates in clinical pharmacy
Junhao JIANG ; Jianyong YUAN ; Xian YU ; Baogang XIE ; Shuang LIANG ; Bo YAN ; Chao YU ; Tao FENG
Chinese Journal of Medical Education Research 2019;18(12):1199-1201
In this study, the necessity of professional master's degree training in clinical pharmacy was analyzed by means of literature research and practice summary, and the existing problems in current professional curriculum, tutor team and practice teaching were discussed. In view of the existing problems, this paper puts forward that medical colleges and universities should give full play to their medical resources, draw lessons from the talents training experience of clinical pharmacists training base, adopt measures such as optimizing curriculum system, selecting tutors, attaching importance to practical teaching, so as to improve the quality of postgraduate training in clinical pharmacy, train high-level clinical pharmacists, and promote the development of clinical pharmacy in China.
10.Application and optimization of CRISPR/Cas system in bacteria.
Junhao FU ; Fayu YANG ; Haihua XIE ; Feng GU
Chinese Journal of Biotechnology 2019;35(3):341-350
Clustered regular interspaced short palindromic repeats (CRISPR) system has been widely used in recent years. Compared with traditional genome editing technology, CRISPR/Cas system has notable advantages, including high editing efficiency, high specificity, low cost and the convenience for manipulation. Type Ⅱ and Ⅴ CRISPR/Cas system only requires a single Cas9 protein or a single Cpf1 protein as effector nucleases for cutting double-stranded DNA, developed as genome editing tools. At present, CRISPR/Cas9 technology has been successfully applied to the genome editing of eukaryotes such as zebrafish, mice and human cells, whereas limited progress has been made in the genome editing of bacteria. In our review, we describe CRISPR/Cas system, its mechanism and summarize the optimization and progress of genome editing in bacteria.
Animals
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Bacteria
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CRISPR-Cas Systems
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Endonucleases
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Gene Editing
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Humans
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Mice