Objective To analyse the association of apolipoprotein E genotype with cerebral infarction and myocardial infarction in the old population of Urumqi. Methods The polymerase chain reaction and restriction fragment length polymorphism technique were used to detect the distribution of genotype and gene frequency of ApoE alleles in 56 cases of cerebral infarction(CI), 60 cases of myocardial infarction (MI) and 104 healthy subjects as control. Results The frequency of ApoE ?3/?4 and ?4/?4 genotypes in CI and MI groups was higher than that in control group(P

Objective To analyse the correlation of apolipoprotein E genotype and Alzheimer's disease(AD) in old population in Urumqi.Methods The polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP) techniques were used to detect the distribution of genotype, gene frequency of ApoE alleles in 60 cases with sporadic AD and 90 normals as control.Results The frequency of ApoE?2, ApoE?3, ApoE?4 was 6.67%, 75.83% and 17.50% respectively in AD group, while in control group, it was 13.33%, 79.45% and 7.22% respectively. The frequency of ApoE?4 allele in AD group was higher than that in control group( P

Curriculum reform is the focus and the key in teaching reforms.The paper is to discuss the shortcomings existing in the present curriculum system in pharmaceutical education and to introduce some exploration and practice in pharmaceutical curriculum reform such as restructuring curriculum.

The authors have made an investigation on the conditions of the internet access,the platform for network-aided teaching,the teaching resource and the teaching effect for physical chemistry among 127 pharmacy students of 2008 enrollment.Based on the results of the investigation,an analysis on the current situation and problems of network-aided teaching for this course was performed and recommendations were put forward accordingly.

Objective To study the perioperative morbidity and mortality of thoraco-abdominal aortic aneurysm ( TAA ) and analyze the relationship between the preoperative or intraoperative risk factors and the morbidity or mortality. MethodsTwenty-three TAA cases undergoing surgery between Jan. 1993 and Dec. 2001 were enrolled. Preoperative cardial, pulmonary, hepatic and renal function, the site and period of intraoperative aortic clamping as well as the emergency operation were taken into account to evaluate independent determinants of the perioperative morbidity and mortality. ResultsThe perioperative morbidity and mortality were 87.0% and 30.4% respectively. As to the morbidity, pulmonary, hepatorenal dysfunction and paraplegia are commonly seen in perioperative period of TAA. Acute renal failure is the most important cause of perioperative death. ConclusionsIntraoperative aortic blocking and massive blood transfusion are the independent determinants influencing perioperative adverse events significantly.

Objective To study operative morbidity and mortality of infrarenal abdominal aortic aneurysm (IAAA) and analyze the correlation between the preoperative or intraoperative risk factors and the morbidity or mortality. Methods Between Jan 1993 and Dec 2001, 120 IAAA cases undergoing surgery were analyzed. Preoperative cardiac, pulmonary, hepatic and renal condition, time of intraoperative aortic clamping as well as emergent operation were taken into account to evaluate the independent determinants of operative morbidity and mortality. Results The operative morbidity was 56.7% and mortality was 10.0%, with the mortality of elective surgery of 5.4% and emergent surgery of 66.7%. Conclusion Preoperative coronary heart disease, hypertention, renal dysfunction and massive blood transfution intra- or postoperatively adversely influenced the postoperative outcome significantly.

Objective To define the significance of the anatomical division and features of popliteal artery in popliteal angioplasty.Methods This study involved 118 lower limbs in 113 cases of femoropoliteal occlusive disease undergoing angioplasty in our center between August 2011 and July 2013.A bent-knee angiogram was taken before recanalization of the lesion.The flexion pattern and the location of flexion points of popliteal artery were collected to study their relationship with the division of popliteal artery.A post-procedural bent-knee angiogram was taken in case of popliteal stenting to assess any change in flexion angulation and distal flow.Results There are diverse flexion patterns in popliteal artery,75.4％ of the flexion points locate in P2 segment,18.6％ in P1,and 6.0％ in P3.On post-procedural bent-knee angiogram in 73 cases of popliteal stenting,10 had a more acute distal flexion angulation,distal artery kinking was observed in another 6 cases of which 5 had compromised distal flow.Artery kinking only occurred in cross-flexion-point stenting.Conclusions A better understanding of the division and anatomical features of popliteal artery can be obtained via bent-knee angiogram.This is of value to angioplasty for lesions in different popliteal divisions.

Objective To study the expression of polycystin1 and polycystin2 in dissection aorta specimens by Real time PCR and immunohistochemisty. Methods Twelve descending dissection aorta specimens from patients of acute Stanford B dissection were taken during surgery; 12 normal descending aorta specimens were taken from multi-organ donors. The G APDH gene was used as control, gene expression of polycystinl and polycystin2 were compared in these two groups with Real time PCR. The expression of polycystinl and polycystin2 protein were showed with immunohistochemisty in the specimen of these two groups. Three high magnify fields were randomly chosen to count the expression of polycystin1 and polycystin2 protein. The counting of polycystin1 and polycystin2 protein were compared between these two groups. Results The gene expression of polycystinl in AD group is 0. 32 fold as that in the control group (P ＜ 0.01 ) and polyeystin2 in AD group is 0.34 fold as that in the control group (P ＜0.01 ); The protein expression of polyeystinl was 0.47 folds as that in the control group ( P ＜ 0.05 ) and polycystin2 in AD group is 0.35 folds as that in the control group ( P ＜ 0.01 ). Conclusions The expression of polycystin1 and polycystin2 on gene and protein level were down regulated in AD group. The down regulation of polycystinl and polycystin2 may play a pivotal role in the development of AD.

Recently, Clinical pharmacy becomes a new specialty of higher education in China. Therefore, building clinical pharmacy teaching quality monitoring system (CPTQMS) is very important not only for improving the teaching quality but also for training inter-disciplinary talents. We try to discuss the connotation and practice of CPTQMS from the objectives, measures and information system. We hope our work can present valuable suggestions for the construction and improvement of CPTQMS in Chinese higher education.

Objective To study the influences of long-term cryopreservation on nucleated cell viability,colony-form- ing units-granulocyte-macrophage(CFU-GM)-forming capability and Lymphocyte immune-phenotypes of pe- ripheral blood hematopoietic stem/progenitor cell(HS/PC)collections.Methods Fresh samples(n=12)were as control,frozen samples were divided into two groups:group 1(cryopreserved for 3-5 years,n=26)and group 2 (cryopreserved for 5-7 years,n=9).Flow cytometry(FCM)method based on 7 AAD or caspase-3staining was ap- plied to assess the degree of cell necrosis or apoptosis in cryopreserved and red-cell lytic collection samples.Lympho- cyte immune-phenotypes(CD3/CD4/CD8/CD19/CD16+56,CD4/CIM5RA/CIMSRO,CD8/CD28)were also de- tected by FCM.CFU-GM culture was done to study the hematopoietic activity.Results Percentages of necrosis and apoptosis cells of the control were both less than the groupl and group2(P