1.Study on User Interface of Pathology Picture Archiving and Communication System.
Dasueran KIM ; Peter KANG ; Jungmin YUN ; Sung Hye PARK ; Jeong Wook SEO ; Peom PARK
Healthcare Informatics Research 2014;20(1):45-51
OBJECTIVES: It is necessary to improve the pathology workflow. A workflow task analysis was performed using a pathology picture archiving and communication system (pathology PACS) in order to propose a user interface for the Pathology PACS considering user experience. METHODS: An interface analysis of the Pathology PACS in Seoul National University Hospital and a task analysis of the pathology workflow were performed by observing recorded video. Based on obtained results, a user interface for the Pathology PACS was proposed. RESULTS: Hierarchical task analysis of Pathology PACS was classified into 17 tasks including 1) pre-operation, 2) text, 3) images, 4) medical record viewer, 5) screen transition, 6) pathology identification number input, 7) admission date input, 8) diagnosis doctor, 9) diagnosis code, 10) diagnosis, 11) pathology identification number check box, 12) presence or absence of images, 13) search, 14) clear, 15) Excel save, 16) search results, and 17) re-search. And frequently used menu items were identified and schematized. CONCLUSIONS: A user interface for the Pathology PACS considering user experience could be proposed as a preliminary step, and this study may contribute to the development of medical information systems based on user experience and usability.
Diagnosis
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Information Systems
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Medical Records
;
Pathology*
;
Radiology Information Systems
;
Seoul
;
Task Performance and Analysis
;
User-Computer Interface
;
Workflow
2.RepWeb: A Web-Based Search Tool for Repeat-Related Literatures.
Taeha WOO ; Younguk KIM ; Jekeun KWON ; Jungmin SEO
Genomics & Informatics 2007;5(2):88-91
Repetitive sequences such as SINE, LINE, and LTR elements form a major part of eukaryotic genomes. A literature search tool that summarizes the information contained within repeat elements would provide biologists in the field of genomics with a useful tool for analyzing genomic sequence features. We developed a java program designed to make literature access easier by using two search engines simultaneously. RepWeb is a web-based search system that provides a user friendly interface for searching the reference data and journals for information related to repeat elements by using the search engines, Google Scholar and PubMed, simultaneously. It provides an interface that displays the repeat element- related biological information, and includes useful functions such as the production of a repeat tree, clickable links to PubMed and Google Scholar, exporting, and sorting a field into date, author, journal and title.
Genome
;
Genomics
;
Indonesia
;
Repetitive Sequences, Nucleic Acid
;
Search Engine
3.A Case of Community-Acquired Methicillin-Resistant Staphylococcus aureus Pneumonia.
Yong Hoon LEE ; Seung Ick CHA ; So Yeon LEE ; Sun Ha CHOI ; Serim OH ; Hyewon SEO ; Jungmin KIM
Korean Journal of Medicine 2013;84(5):728-732
Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) has received considerable attention in recent years as the cause of infections in individuals in the community who do not have traditional risk factors for MRSA infection, such as hospitalization or contact with healthcare services. CA-MRSA strains have different molecular and antimicrobial susceptibilities, as compared to hospital-associated MRSA. Although CA-MRSA strains are primarily associated with skin and soft tissue infections, they can cause more invasive infections, such as severe community-acquired pneumonia. Reports on CA-MRSA pneumonia in Korea are sparse. Therefore, we report a case of CA-MRSA pneumonia with molecular typing of the MRSA isolate.
Community-Acquired Infections
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Delivery of Health Care
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Hospitalization
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Korea
;
Methicillin Resistance
;
Methicillin-Resistant Staphylococcus aureus
;
Molecular Typing
;
Pneumonia
;
Risk Factors
;
Skin
;
Soft Tissue Infections
4.Effects of Lipoxygenase Inhibitor on Diabetic Nephropathy in Rats: Decreasing Proteinuria and Preserving Renal Function.
Hyun Chul CHUNG ; Il Young KIM ; Seo Rin KIM ; Jungmin SON ; Dong Won LEE ; Sang Heon SONG ; Eun Young SEONG ; Ihm Soo KWAK ; Soo Bong LEE
Korean Journal of Nephrology 2011;30(5):452-458
PURPOSE: Oxidative stress leads to an increased production of lipoxygenase derivatives in diabetic nephropathy. Thus, we hypothesized that lipoxygenase inhibitor, nordihydroguaiaretic acid (NDGA), ha the effects of decreasing proteinuria and preserving renal function in streptozotocin (STZ)-induced diabetic rats. METHODS: 45 Sprague-Dawley rats were divided into three groups; (A) treatment with lipoxygenase inhibitor, NDGA in diabetic nephropathy rats, (B) treatment with dimethyl sulfoxide (DMSO) as a vehicle in STZ-induced diabetic rats, (C) normal control group with subcutaneous injection of normal saline. Diabetes was induced by a single intraperitoneal injection of STZ (65 mg/kg) in rats of group A and B. After the 4th week of STZ injection, NDGA (10 mg/kg) and DMSO were given subcutaneously for another 4 weeks in group A and B respectively. RESULTS: The NDGA-treated diabetic rats exhibited significantly decreased urinary albumin excretion. Serum creatinine and blood urea nitrogen concentrations were increased in both group A and B, and tend to be higher in group B than group A. Twenty-four-hour urine creatinine clearances were increased in both group A and B after injection of STZ. Pathologic alterations of kidney were observed after injection of STZ, and then attenuated after administration of NDGA. CONCLUSION: These results suggest the potential of lipoxygenase inhibitor as a complementary therapy for the prevention and treatment of diabetic nephropathy.
Animals
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Blood Urea Nitrogen
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Creatinine
;
Diabetic Nephropathies
;
Dimethyl Sulfoxide
;
Injections, Intraperitoneal
;
Injections, Subcutaneous
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Kidney
;
Lipoxygenase
;
Nordihydroguaiaretic Acid
;
Oxidative Stress
;
Proteinuria
;
Rats
;
Rats, Sprague-Dawley
;
Safrole
;
Streptozocin
5.Antimicrobial Resistance and Multilocus Sequence Typing of Vancomycin-Resistant Enterococcus faecium Isolated from Clinical Specimens.
Jae Young OH ; Sung Ho HER ; Sung Yong SEO ; Yoo Chul LEE ; Je Chul LEE ; Jungmin KIM ; Dong Taek CHO
Journal of Bacteriology and Virology 2008;38(1):19-27
A total of 58 vancomycin-resistant E. faecium (VREF) was isolated from 3 hospitals located in Daegu, Korea. The VREF isolates were evaluated for the antimicrobial susceptibility pattern and resistance determinants against vancomcin, aminoglycosides, and macrolides. The multilocus sequence types (MLST) were determined to characterize the clonal diversity of the VREF isolates. The VREF isolates were highly resistance to teicoplanin, erythromycin, ciprofloxacin, gentamicin, and streptomycin, whereas quinupristin-dalfopristin and linezolid were the most susceptible drugs. All isolates carried the vanA gene. The aac6'-aph2" (n=53) and aadE (n=27) genes were detected in the high-level aminoglycoside resistant (HLAR) isolates. The aac6'-aph2" gene was located in the conjugally transferable plasmids. The ermB and ermA genes were detected in the 54 and 3 VREF isolates, respectively. The VREF isolates showed 11 different sequence types (ST). The VREF isolates belonging to ST192 was the most prevalent (n=19), but detected in one hospital, whereas the isolates belonging to ST203 (n=11) were detected in 3 hospitals. These results suggest that the VREF isolates resistant to aminoglycosides and erythromycin are originated from different clones and specific VREF clones are spread in the study hospitals.
Acetamides
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Aminoglycosides
;
Ciprofloxacin
;
Clone Cells
;
Enterococcus
;
Enterococcus faecium
;
Erythromycin
;
Gentamicins
;
Korea
;
Linezolid
;
Macrolides
;
Multilocus Sequence Typing
;
Oxazolidinones
;
Plasmids
;
Streptomycin
;
Teicoplanin
;
Virginiamycin
6.ManBIF: a Program for Mining and Managing Biobank Impact Factor Data.
Ki Jin YU ; Jungmin NAM ; Yun HER ; Minseock CHU ; Hyungseok SEO ; Junwoo KIM ; Jaepil JEON ; Hyekyung PARK ; Kiejung PARK
Genomics & Informatics 2011;9(1):37-38
Biobank Impact Factor (BIF), which is a very effective criterion to evaluate the activity of biobanks, can be estimated by the citation information of biobanks from scientific papers. We have developed a program, ManBIF, to investigate the citation information from PDF files in the literature. The program manages a dictionary for expressions to represent biobanks and their resources, mines the citation information by converting PDF files to text files and searching with a dictionary, and produces a statistical report file. It can be used as an important tool by biobanks.
Mining
7.PrimateDB: Development of Primate Genome DB and Web Service.
Taeha WOO ; Gwangsik SHIN ; Taewook KANG ; Byoungchul KIM ; Jungmin SEO ; Sang Soo KIM ; Chang Bae KIM
Genomics & Informatics 2005;3(2):73-76
The comparative analysis of the human and primate genomes including the chimpanzee can reveal unique types of information impossible to obtain from comparing the human genome with the genomes of other vertebrates. PrimateDB is an open depository server that provides primate genome information for the comparative genome research. The database also provides an easy access to variable information within/between the primate genomes and supports analyzed information, such as annotation and retroelements and phylogeny. The comparative analyses of more primate genomes are also being included as the long-term objective.
Genome*
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Genome, Human
;
Humans
;
Pan troglodytes
;
Phylogeny
;
Primates*
;
Retroelements
;
Vertebrates
8.REPEATOME: A Database for Repeat Element Comparative Analysis in Human and Chimpanzee.
Taeha WOO ; Tae Hui HONG ; Sang Soo KIM ; Won Hyong CHUNG ; Hyo Jin KANG ; Chang Bae KIM ; Jungmin SEO
Genomics & Informatics 2007;5(4):179-187
An increasing number of primate genomes are being sequenced. A direct comparison of repeat elements in human genes and their corresponding chimpanzee orthologs will not only give information on their evolution, but also shed light on the major evolutionary events that shaped our species. We have developed REPEATOME to enable visualization and subsequent comparisons of human and chimpanzee repeat elements. REPEATOME (http://www.repeatome.org/) provides easy access to a complete repeat element map of the human genome, as well as repeat element-associated information. It provides a convenient and effective way to access the repeat elements within or spanning the functional regions in human and chimpanzee genome sequences. REPEATOME includes information to compare repeat elements and gene structures of human genes and their counterparts in chimpanzee. This database can be accessed using comparative search options such as intersection, union, and difference to find lineage-specific or common repeat elements. REPEATOME allows researchers to perform visualization and comparative analysis of repeat elements in human and chimpanzee.
Genome
;
Genome, Human
;
Humans*
;
Pan troglodytes*
;
Primates
9.Novel Mechanism of Action of Dutasteride for Inducing Hair Growth in Patients with Female-Pattern Hair Loss
SooJung KIM ; Yul-Lye HWANG ; Su-Hyuk YIM ; Dongkyun HONG ; Chong Won CHOI ; Kyung Eun JUNG ; Young-Joon SEO ; Chang-Deok KIM ; JungMin SHIN ; Young LEE
Korean Journal of Dermatology 2020;58(4):231-238
Background:
Female-pattern hair loss (FPHL) is a common hair loss disorder in women. The various treatments include topical minoxidil and 17α-estradiol, as well as oral anti-androgens. However, the clinical efficacy of 5α -reductase inhibitors remains controversial.
Objective:
We evaluated the clinical utility of dutasteride in FPHL patients and how dutasteride promotes hair growth.
Methods:
We evaluated hair follicle density and thickness before and after oral dutasteride treatment in 24 patients with FPHL. We measured β-catenin activity in primary cultures of human dermal papilla cells (DPCs) using the TOP Flash reporter assay and Western blotting. The expression levels of genes promoting hair growth were quantitatively assessed using quantitative polymerase chain reaction (Q-PCR).
Results:
The mean vertex hair density increased significantly from 67±14 to 76±13/cm2 (p=0.001) and the mean occipital hair density increased from 89±11 to 94±13/cm2 (p=0.012) after dutasteride treatment. However, the mean hair thickness did not increase. When DPCs were treated with dutasteride, TOP Flash activity increased in a dose-dependent manner, and the protein level of non-phosphorylated (active) β-catenin also increased. The mRNA level of vascular endothelial growth factor increased, but the mRNA levels of the keratinocyte growth factor, insulin growth factor-1, and Noggin were not affected by dutasteride.
Conclusion
This study shows a novel mechanism of dutasteride in promoting hair growth and provides support for the possible clinical application of 5α-reductase inhibitors for the treatment of FPHL.
10.Restoration of Immune Privilege in Human Dermal Papillae Controlling Epithelial-Mesenchymal Interactions in Hair Formation
Jung Min PARK ; Mee Sook JUN ; Jung-A. KIM ; Nanda Maya MALI ; Tsai-Ching HSI ; Areum CHO ; Jung Chul KIM ; Jun Young KIM ; Incheol SEO ; Jungmin KIM ; Moonkyu KIM ; Ji Won OH
Tissue Engineering and Regenerative Medicine 2022;19(1):105-116
BACKGROUND:
Hair follicles are among a handful of organs that exhibit immune privilege. Dysfunction of the hair follicle immune system underlies the development of inflammatory diseases, such as alopecia areata.
METHODS:
Quantitative reverse transcription PCR and immunostaining was used to confirm the expression of major histocompatibility complex class I in human dermal papilla cells. Through transcriptomic analyses of human keratinocyte stem cells, major histocompatibility complex class I was identified as differentially expressed genes. Organ culture and patch assay were performed to assess the ability of WNT3a conditioned media to rescue immune privilege. Lastly, CD8? T cells were detected near the hair bulb in alopecia areata patients through immunohistochemistry.
RESULTS:
Inflammatory factors such as tumor necrosis factor alpha and interferon gamma were verified to induce the expression of major histocompatibility complex class I proteins in dermal papilla cells. Additionally, loss of immune privilege of hair follicles was rescued following treatment with conditioned media from outer root sheath cells. Transcriptomic analyses found 58 up-regulated genes and 183 down-regulated genes related in MHC class I? cells. Using newborn hair patch assay, we demonstrated that WNT3a conditioned media with epidermal growth factor can restore hair growth. In alopecia areata patients, CD8? T cells were increased during the transition from mid-anagen to late catagen.
CONCLUSION
Identification of mechanisms governing epithelial and mesenchymal interactions of the hair follicle facilitates an improved understanding of the regulation of hair follicle immune privilege.