In the present study, we examined to determine the development of various lymphoid tissue including mucosa-associated lymphoid tissue (MALT), thymus, lymph node and liver. In order to investigate the relationship between the morphological events and the expression pattern of MIC2 antigen (CD99) during the development of lymphoid system, we performed the immunohistochemical study using DN16, a monoclonal antibody against MIC2 (CD99), on formalin-fixed and paraffin-embedded lymphoid sections in 68 human embryos and fetuses, between 5 and 39 gestational week (GW). Four neonates, an infant, and 5 adults are also included. CD99 has been expressed along the membrane of hepatocytes and sinusoidal endothelial cells for 10-28 GW, in when the liver the major site of hematopoiesis. In the thymus, CD99 was firstly detected in the presumptive epitheial cells at 10 GW. When the thymus matured and corticomedullary differentiation appeared, CD99 was exclusively expressed in cortical thymocytes. The CD99 expression in epithelial cells of MALT has initiated at 6 GW and 10 GW earlier than that at the onset of MALT development and its expression has been persisted during MALT formation especially 16-25 GW. The finnding that CD99 antigen was expressed in epithelial cells during the development of MALT rnight provide a means to identify a novel epithelial differentiated substance. In addition, endothelial cells that are present in various organs such as liver and small intestine concurrently expressed CD99 antigen and its expression persisted to late fetal period. This point rnight suggest that CD99 antigen regulate the irnigration of lymphocytes from liver, major hematopoietic organ, to thymus or peripheral lyrnphoid organ via the interaction between endothelial cells and lymphocytes.
Adult ; Embryonic Structures* ; Endothelial Cells ; Epithelial Cells ; Fetus* ; Hematopoiesis ; Hepatocytes ; Humans* ; Infant ; Infant, Newborn ; Intestine, Small ; Liver ; Lymph Nodes ; Lymphocytes ; Lymphoid Tissue* ; Membranes ; Thymocytes ; Thymus Gland

Glomus tumor is well recognized and documented lesion which usually presents as painful dermal nodules in fingers. The tumor results from hypertrophy of a glomus, which is a normal structure of the skin, a coiled arterioyenous shunt regulating body temperature. Glomus tumor may occur at any age but is most frequent in the middle age of life. Pain, tenderness and cold sensitivity are the classic triad of symptoms. Complete surgical exision is the treatment of choice for glomus tumors. Successful removal of tumor leads to complete relief of pain and return to normal function. We report 4 cases of subungual glomus tumor successfully treated by complete exision with review of literatures.
Body Temperature ; Fingers ; Glomus Tumor ; Humans ; Hypertrophy ; Middle Aged ; Skin

PURPOSE: To evaluate the nephrotoxicity of Gd-DTPA in rats with normal renal function and in those with acute renal failure, using 0.1mmol/kg and 0.3mmol/kg in each group. MATERIALS AND METHODS: Sixty rats weighing 200-250gm were divided into two groups: normal (N) and glycerol induced acute renal failure (F) ; this was inducedby intramuscular injection of 50% glycerol(10ml/kg). Each group was divided into three subgroups; subgroup 1 was given physiologic saline (0.15 M NaCl) via the tail vein, while subgroups 2 and 3 were given 0.1 mmol/kg Gd-DTPAand 0.3mmol/kg Gd-DTPA, respectively. Blood and 24-hour urine were collected before and on the first and secondday after intravenous injection of either physiologic saline or Gd-DTPA. In order to obtain pathologic specimens, two additional rats in each group underwent the same experimental procedure, and on the first or second day after intravenous injection of saline or Gd-DTPA, both kidneys were removed for light microscopic examination. Serum creatinine (Cr), urine creatinine, and several urinary enzymes such as alkaline phosphatase (ALP), gammaglutamyl transferase (GGT), N-acetyl-Beta-glucosaminidase (Beta-NAG) and lactic dehydrogenase (LDH) of 24 hour urine were measured, and creatinine clearance (Ccr) was calculated. RESULTS: The results were as follows. 1. In the groupwith normal renal function, levels of serum creatinine and urinary enzymes remained unchanged after infusion of 0.1mmol/kg Gd-DTPA or 0.3mmol/kg Gd-DTPA. 2. Among rats with acute renal failure, no difference in serum creatinine and urinary enzyme levels between the saline injection group and 0.1mmol/kg Gd-DTPA injection group was noted. 3. Among rats with acute uremia, Beta-NAG, LDH and ALP were significantly higher on post-injection days 1 and 2 in the 0.3mmol/kg Gd-DTPA injection group than in the physiologic saline or 0.1mmol/kg Gd-DTPA injection group.4. Light microscopic exammination of rats with normal renal function after Gd-DTPA injection revealed no significant pathologic change. Those with acute renal failure rats revealed some degree of vacuolization and/or necrosis of renal proximal tubular epithelial cells. No difference was found, however, between the saline and Gd-DTPA injection subgroups. CONCLUSION: In conclusion, Gd-DTPA was safe in rats with normal renal function, even when the dose was tripled (0.3mmol/kg), and was safe in those with acute renal failure when the standard dose (0.1mmol/kg) was given. In this latter group, however, a triple dose induced some renal damage.
Acute Kidney Injury* ; Alkaline Phosphatase ; Animals ; Creatinine ; Epithelial Cells ; Gadolinium DTPA* ; Glycerol ; Injections, Intramuscular ; Injections, Intravenous ; Kidney* ; Necrosis ; Oxidoreductases ; Rats* ; Transferases ; Uremia ; Veins

PURPOSE: The purpose of this study was to explore the clear concept of organizational commitment for effective nursing personnel management. METHOD: This study was conducted using Walker & Avant's conceptual analysis framework(2005). RESULTS: Organizational commitment was identified with six attributes: acknowledgment, acceptance, trust, sacrifice, attachment, regulation. Antecedents of organizational commitment were personal characteristics, perceived organizational support, empowerment, fair compensation, job satisfaction, job autonomy, organizational culture, and leadership. Consequences of organizational commitment were turnover intention, job performance and organizational citizenship behavior. CONCLUSION: Organization commitment is a core concept for understanding the management of nursing personnel. Appropriate instruments to operationalize the concept of organizational commitment including six attributes need to be developed.
Compensation and Redress ; Humans ; Intention ; Job Satisfaction ; Leadership ; Nursing* ; Organizational Culture ; Personnel Management ; Power (Psychology)

Drash syndrome, which was first reported by Denys et al. in 1967 is a complex disorder which associates a nephropathy, Wilms' tumor, and male pseudohermaphroditism. The common denominator is a nephropathy. The nephropathy may be associated with either genital abnormalities or Wilms' tumor, and these associations are called incomplete form of Drash syndrome. This syndrome appears early in life and the first sign usually is genital ambiguity. The nephropathy presents with proteinuria, hematuria and hypertension, and eventually progresses to end stage renal failure. Renal biopsy may reveal a variety of glomerular and interstitial changes. Wilms' tumor may appear as s mass on ultrasound or it may not be recognized until nephrectomy or even autopsy. We report on a boy with nephropathy and genital abnormalities. A nephrotic syndrome with hypertension was present when first seen at 15 days of age. The karyotype was 46, XY and external genitalia was ambiguous. The nephrotic syndrome and signs of renal insufficiency persisted and he died at the age of 40 days. Histopathologic findings of kidney at autopsy revealed those of diffuse mesangial sclerosis. The case was presented with brief review of literatures.
46, XY Disorders of Sex Development ; Autopsy ; Biopsy ; Denys-Drash Syndrome* ; Disorders of Sex Development ; Genitalia ; Hematuria ; Humans ; Hypertension ; Karyotype ; Kidney ; Male ; Nephrectomy ; Nephrotic Syndrome ; Proteinuria ; Renal Insufficiency ; Sclerosis ; Ultrasonography ; Wilms Tumor

PURPOSE: This study was designed to examine effects of sense of calling, job satisfaction and organizational commitment on retention intention in nurses who are at the advanced beginner-stage. METHODS: Participants were 199 nurses with 13 to 36 months' clinical career at hospital located in Busan, Korea. Data were analyzed using descriptive statistics, independent t-test, one-way ANOVA, Scheffé test, Pearson correlation, and Multiple regression. RESULTS: Retention intention correlated positively with sense of calling (r=.43, p<.001), job satisfaction (r=.31, p<.001) and organizational commitment (r=.31, p<.001). Factors affecting the participants' retention intention were found to be statistically significant (F=21.96, p<.001). Total factor score accounted for 24.1% of retention intention. Out of the related factors, the most influential factor was 'goals/meaning', belonging to the sub-areas of the sense of calling and explaining 18.2% of retention intention, followed by organizational commitment and average monthly income in that order. CONCLUSION: The results of this study suggest a need to develop a management program that helps promote retention intention for nurses at the advanced beginner-stage by both improving their monthly income and outside conditions and, more importantly, raising their sense of calling, especially in association with the goals/meaning of their life.
Busan ; Intention* ; Job Satisfaction* ; Korea

No abstract available.
Pulmonary Artery* ; Takayasu Arteritis*

We report a case of gastro-colic fistula caused by ectopic gastric mucosa developed at transverse colon. Fistula was detected by colonofiberscopy. And fistulous tract was proved by barium enema. Meckel's diverticulum scan finding was similar to that of GI bleeding; e.g. injected radioactivity was secreted into the lumen and moved along the lumen. There was no bleeding. And there was no diverticulum in the colon. Absence of diverticular pouch may explain this unusuaal GI bleeding-like scan finding rather than focal collection of radioactivity, which is typical of ectopic gastric mucosa found in the Meckel's diverticulum. Ectopic gastric mucosa was confirmed by colonfiberscopic biopsy. We suggest GI bleeding-like pictures should be included differential diagnosis of Tc-99m-O4 (ectopic gastric mucosa or Meckel's diverticulum) scan.
Barium ; Biopsy ; Colon ; Colon, Transverse ; Diagnosis, Differential ; Diverticulum ; Enema ; Fistula* ; Gastric Mucosa* ; Hemorrhage ; Meckel Diverticulum ; Radioactivity ; Radionuclide Imaging ; Sodium Pertechnetate Tc 99m*

OBJECTIVES: Cadmium (Cd), a toxic and non-essential metal, is recognized as a human carcinogen, which has a tendency to accumulate in the human body. The levels of Cd in renal cortex and liver are good indicators as an index of Cd exposure in the general population. In this study, we present an estimation of reference Cd levels in tissue (renal cortex and liver) and total body burden in the general population of Korea. MEDTHODS: Cd and zinc (Zn) were analyzed in renal cortex and liver from 254 autopsies (male : 188 cases, female : 66 cases) aged 0 to 87 years. RESULTS: Geometric mean concentration of Cd was 27.4 and 3.1 ua/g wet weight in renal cortex and liver, respectively. The level of Zn in renal cortex and liver was 35.4 and 42. 6 v/g wet weight, respectively. The result suggests that kidney is the target organ for Cd accumulation. The accumulation of Cd in renal cortex was age-dependent with a biphasic pattern. The level of Cd in renal cortex increased with age up to the fifties, and then leveled off thereafter. Based on the data, the regression model for Cd accumulation in renal cortex by age is predicted by : Log KCd = 0. 2325 + 0. 0553 Age 0. 0005 Age. The highest Cd accumulation in renal cortex of Koreans was estimated at 43. 3 ua/g wet weight at 50. 8 years old. In addition, the total Cd body burden by age was estimated by the following equation: Total Cd Body Burden = -4. 5948 + l. 2278 Age - 0. 0121 Age. The highest body burden of Cd was estimated at 26. 5 mg at age 50. 7 years in the Korean general population. The positive correlation between Zn and Cd was observed in renal cortex and liver. CONCLUSIONS: The level of Cd exposure in Korean was found to be lower than in Japanese, but same as or higher than in American and Europeans.
Asian Continental Ancestry Group ; Autopsy ; Body Burden ; Cadmium* ; Child ; Female ; Human Body ; Humans ; Kidney Cortex ; Kidney* ; Korea ; Liver* ; Reference Values* ; Zinc

Thirty five male Sprague-Dawley rats were treated with cadmium chloride solution ranging from 0.2 to 3.2mg CdCl2/kg by intravenous single injection. At 48 hours after administration of cadmium, total cadmium, MT bound cadmium and histopathologic finding in liver, kidney, lung, heart, testis, metallothionein in liver, kidney and total cadmium in blood were examined. Tissue cadmium concentration was highest in liver, followed by in kidney, heart, lung and testis. Cadmium bound to metallothionein(MT-Cd) and ratio of MT-Cd to total cadmium were increased in liver and kidney dependently of cadmium exposure dose, but not significantly changed in other organs. On histopathologic finding, the most susceptible organ was heart in considering cadmium exposed dose, but testis in considering cadmium concentration. Blood cadmium concentration was increased with dose-dependent pattern, and significantly correlated with tissue cadmium concentration, so that we may estimate tissue cadmium concentration by measurement of blood cadmium concentration. Metallothionein in liver and kidney was increased with dose-dependent pattern, higher in liver than in kidney, and was significantly correlated with tissue cadmium concentration. However, metallothionein induction efficiency of tissue cadmium(microgram MT/microgram Cd) was greater in liver than in kidney, and reverse to tissue concentration or exposed dose of cadmium.
Animals ; Cadmium Chloride ; Cadmium* ; Heart ; Humans ; Kidney ; Liver ; Lung ; Male ; Metallothionein* ; Rats* ; Rats, Sprague-Dawley ; Testis