1.Cloning and Expression of Low Molecule Protein Antigens from Mycobacterium tuberculosis H37Rv.
Jae Hyun LIM ; Hwa Jung KIM ; Eun Kueong JO ; Chang Hwa SONG ; Un Ok KIM ; Jeong Kyu PARK
Journal of the Korean Society for Microbiology 2000;35(5):375-375
No Abstract Available.
Clone Cells*
;
Cloning, Organism*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
2.A Pilot Survey of Difficult Intubation and Cannot Intubate, Cannot Ventilate Situations in Korea.
Jung Soo KIM ; Hyun Kyoung LIM ; Jeong Yun SONG ; Hyun Keun LIM ; Kyungchul SONG ; Jae Hwa CHO
Korean Journal of Critical Care Medicine 2016;31(3):202-207
BACKGROUND: There have been no studies of airway management strategies for difficult intubation and cannot intubate, cannot ventilate (CICV) situations in Korea. This study was intended to survey devices or methods that Korean anesthesiologists and intensivists prefer in difficult intubation and CICV situations. METHODS: A face-to-face questionnaire that consisted of a doctor's preference, experience and comfort level for alternative airway management devices was presented to anesthesiologists and intensivists at study meetings and conferences from October 2014 to December 2014. RESULTS: We received 218 completed questionnaires. In regards to difficult intubation, the order of preferred alternative airway devices was a videolaryngoscope (51.8%), an optical stylet (22.9%), an intubating laryngeal mask airway (11.5%), and a fiber-optic bronchoscope (10.6%). One hundred forty-two (65.1%) respondents had encountered CICV situations, and most of the cases were identified during elective surgery. In CICV situations, the order of preferred methods of infraglottic airway management was cricothyroidotomy (CT) by intravenous (IV) catheter (57.3%), tracheostomy by a surgeon (18.8%), wire-guided CT (18.8%), CT using a bougie (2.8%), and open surgery CT using a scalpel (2.3%). Ninety-eight (45%) of the 218 respondents were familiar with the American Society of Anesthesiologists' difficult airway algorithm or Difficult Airway Society algorithm, and only 43 (19.7%) had participated in airway workshops within the past five years. CONCLUSION: The videolaryngoscope was the most preferred device for difficult airways. In CICV situations, the method of CT via an IV catheter was the most frequently used, followed by wire-guided CT method and tracheostomy by the attending surgeon.
Airway Management
;
Bronchoscopes
;
Catheters
;
Congresses as Topic
;
Education
;
Intubation*
;
Intubation, Intratracheal
;
Korea*
;
Laryngeal Masks
;
Methods
;
Surveys and Questionnaires
;
Tracheostomy
3.Vestibuloneuritis Developed Concurrently in Ipsilateral Site with Herpes-Zoster Oticus Syndrome .
Gyu Cheol HAN ; Ju Hyoung LEE ; Joo Hyun WOO ; Jung Kook YOO ; Sun Hwa LIM
Journal of the Korean Balance Society 2004;3(1):187-191
BACKGROUND:Acute vestibular neuronitis is the disease of which the etiology and pathophysiology are largely unknown . But the viral infection and ischemia of the labyrinth and the vestibular nerve are considered as general etiology. This study was performed to support the viral infection rather than the ischemic theory. MATERIALS & METHODS:We studied seventy years old female patient who showed painful vesicles on left auricle and vertigo with spontaneous nystagmus to the right side. We performed physical examination, serologic test, ENG test, pure tone audiogram, brain magnetic resonance imaging and polymerase chain reaction. RESULTS:We found small vesicles and vascular injection in left EAC, herpes zoster IgG positive, spontaneous right beating in electronystagmograpy, 54% left canal paresis in Caloric test , decreasing left side Tc in velocity step rotatory test, decresed gain, deviation to left in symmetry and phase lead in sinusoidal harmonic acceleration test, normal range hearing in pure tone audiogram, microangiopathy on cortex in brain MRI and negative PCR. CONCLUSION:This case supports viral infection etiology rather than ischemia in vestibular neuritis. But more studies to find the etiology of vestibular neuronitis are required.
Acceleration
;
Brain
;
Caloric Tests
;
Ear, Inner
;
Female
;
Hearing
;
Herpes Zoster
;
Herpes Zoster Oticus
;
Humans
;
Immunoglobulin G
;
Ischemia
;
Magnetic Resonance Imaging
;
Paresis
;
Physical Examination
;
Polymerase Chain Reaction
;
Reference Values
;
Serologic Tests
;
Vertigo
;
Vestibular Nerve
;
Vestibular Neuronitis
4.An Assessment of Dietary Fiber Intake in Preschool Children in Busan.
Korean Journal of Community Nutrition 2002;7(2):167-176
To assess the dietary fiber intake of preschool children in Busan and to evaluate the relationship between of the intake of dietary fiber and nutrient intake. Nutrient intake using 24 hour recall, and total dietary fiber (TDF) intake based on tables of TDF of common Korean floods developed by the modified Prosky Method, were estimated for 176 preschool children. The mean daily intakes of TDF, and TDF after adjusting energy intake, were 10.20 g and 7.69 g/1,000 kcal, respectively. The mean daily intakes of TDF for children aged 1-3 and 4-6 years were 9.20 g and 11.08 g, respectively. The range of TDF intake was 1.86 to 22.16 g. The major sources of TDF were cereals (31.0%), vegetables (18.9%) and fruits (11.9%). The TDF intake showed positive correlations with nutrient adequacy ratios (NAR) of iron and Vitamin B1, (p<0.05, p<0.05). The TDF intake per 1,000 kcal showed negative correlations with the NARs of protein, calcium, phosphate, iron, Vitamin A, vitamin B1, Vitamin B2, and niacin (p<0.001, p<0.001, p<0.001, p<0.05, p<0.001, p<0.001, p<0.001, p<0.001) and with the mean adequacy ratio (MAR, p<0.001). When children were stratified into quartiles (Q1-Q4) on the basis of their fiber intake per 1,000 kcal, their NARs for calcium, phosphate, iron, Vitamin A, Vitamin B1, Vitamin B2 and niacin (p<0.05, p<0.05, p<0.001, p<0.05, p<0.05, p<0.001, p<0.001), and their U? (p<0.001) were significantly lower in the children with higher fiber intake per 1,000 kcal (the upper quartile). The NARs fur calcium (0.63), iron (0.60), Vitamin A (0.66), Vitamin B2 (0.74), niacin (0.64), Vitamin C (0.65) and the MAR (0.74) were lower than 0.75 in the children with fiber intakes of more than 9.25 g per 1,000 local (0,), the highest fiber intake per 1,000 kcal. Based on these results, the mean TDF intake of children was higher than the 'age (yr)+5g', the minimum recommended level for American children. Meals with a fiber intake of more than 9.25 g per 1,000 local (Q4) could cause a decreased nutritional status for minerals and vitamins. The result of this study could contribute to the establishment of Recommended Dietary Allowances (RDA) for dietary fiber for Korean Preschool children.
Ascorbic Acid
;
Busan*
;
Calcium
;
Edible Grain
;
Child
;
Child, Preschool*
;
Dietary Fiber*
;
Energy Intake
;
Floods
;
Fruit
;
Humans
;
Iron
;
Meals
;
Minerals
;
Nerve Fibers, Myelinated
;
Niacin
;
Nutritional Status
;
Recommended Dietary Allowances
;
Riboflavin
;
Thiamine
;
Vegetables
;
Vitamin A
;
Vitamins
5.Study on Macrosomia Based on Birth Certificate Data.
Sang Hwa PARK ; Jung Ho HAN ; Kyung Sil LIM ; Seung Yup KU ; Seok Hyun KIM
Korean Journal of Obstetrics and Gynecology 2000;43(9):1611-1615
No abstract available.
Birth Certificates*
;
Parturition*
6.Brain MRI in children with delayed development: emphasis on white matter maturation.
Mi Sook SUNG ; Ok Hwa KIM ; Jung Lim MOON ; Kyung Sub SHINN ; Yong Whee BAHK
Journal of the Korean Radiological Society 1992;28(3):457-462
To analyzed the progression of white matter maturation and white matter pathology, MR imaging of the brain was obtained in 38 children with delayed development. Children with developmental delay showed a high incidence of MR abnormalities(34/38, 89.5%). Delayed pattern of myelination and gray-white matter differentiation was seen in 13 patients. Twenty-two patients had white matter patholgy, including 14 with white matter hypoplasia, seven with focal small infarction, five with periventricular leukomalacia, and three with high signal intensities on T2 weighted image. Associated structural abnormalities were also evaluated. The most common lesions in decreasing frequency were cerebral atrophy and dysgenesis of the corpus callosum, pachygyria and/or polymicrogyria, porencephalic cyst and Leigh's disease. Twenty-three of 34 children had multiple abnormalities on MRI. The MRI was useful in depicting the progression of myelination and other white matter lesions, and serial follow-up MR is recommended for patients with delayed or lack of myelination and gray-white matter differentiation.
Abnormalities, Multiple
;
Atrophy
;
Brain*
;
Child*
;
Corpus Callosum
;
Follow-Up Studies
;
Humans
;
Incidence
;
Infant, Newborn
;
Infarction
;
Leigh Disease
;
Leukomalacia, Periventricular
;
Lissencephaly
;
Magnetic Resonance Imaging*
;
Myelin Sheath
;
Pathology
;
Polymicrogyria
;
White Matter*
7.The Factors Affecting Job Stress and Job Satisfaction of Nurses at Welfare Centers in Seoul.
Hee Ja LIM ; Hye Sun JUNG ; Jung Wan KOO
Journal of Korean Academy of Community Health Nursing 2005;16(3):341-350
PURPOSE: The purpose of this research was to find out factors affecting job stress and job satisfaction of nurses working at the welfare centers. METHODS: This study was performed with 140 nurses who were working at or had retired within 1 year from general welfare centers in Seoul during the period from March 29 to February 29 2004. We used a questionnaire asking about general characteristics. work characteristics. stress factors and job satisfaction. We analysed 103 subjects who replied to the questionnaires. RESULTS: The mean job stress level was 2.84 out of 5 and. as for the sub-factors of job stress. income was 4.19, career development 3.69, service environment 3.26 and support system 1.90. The mean job satisfaction level was 3.32 out of 5 and. as for the sub-factors of job satisfaction, job control was 3.58, efficiency of organization 3.27, job burden and responsibility 2.92 and role ambiguity 2.25. When the nurses' job stress was compared according to their general and work characteristics. stress level was significantly high in nurses who had a short experience. were working at the department of senior welfare center. or were the only staff at their workplace (p<0.05). In addition. satisfaction level was significantly high in nurses who were working at the department of senior welfare center and medical welfare team. were the only staff at their workplace or had a heavy work load (p<0.05). According to the results of multiple regression analysis. job stress level was influenced by age (R2=0.212, p<0.01). and job satisfaction level by the number of staffs and work load (R2=0.272, p<0.1). CONCLUSIONS: The results of the study suggest that good atmosphere should be made up to communicate between the departments at the workplace in order to lessen job stress and enhance job satisfaction of nurses working at welfare centers. In addition. role division and job duties among special staffs should be reviewed in order to achieve high efficiency in performing job duties.
Atmosphere
;
Job Satisfaction*
;
Seoul
;
Surveys and Questionnaires
8.Purification and Partial Characterization of the 38 kDa Glycolipoprotein Antigen from the Culture Filtrate of Mycobacterium tuberculosis H37Rv.
Jeong Kyu PARK ; Hee Chul CHANG ; Jae Hyun LIM ; Chang Hwa SONG ; Un Ok KIM ; Eun Kyeong JO ; Hwa Jung KIM
Journal of Bacteriology and Virology 2001;31(3):249-257
Mycobacterium tuberculosis infected macrophages can become ineffective at activating CD4+ T cells through presentation of peptide antigens by MHC class II, possibly contributing to the ability of M tuberculosis to persist despite the presence of an intact immune system. Presentation of lipid antigens may help to overcome this problem. CD1 represents the key component of an MHC independent pathway for presentation nonpeptide lipid antigens to T cells. The 38 kDa glycolipoprotein antigen of M. tuberculosis is actively secreted. The antigen induces strong antibody and T-cell responses and provided partial protection against M. tuberculosis infection in mice when it is administered either entrapped in biodegradable microparticles or in the form of a DNA vaccine. But an selective anergy to stimulation with peptide of the 38 kDa was observed in the majority of tuberculosis patients. An 38 kDa antigen has been isolated by affinity chromatography using a monoclonal antibody. This antigen contains some immunosuppressive cell wall associated antigens such as lipoarabinomannan. Therefore, we purified the 38 kDa glycolipoprotein from the culture filtrate of M tuberculosis H37Rv by ammonium sulfate precipitation (55~80%), hydroxylapatite and DEAE-Sephacel column. The purified antigen showed three major bands on isoelectric focusing gel, and two-dimensional electrophoresis (2-DE) analysis of this antigen revealed five distinct spots of the 38 kDa molecular mass. One of five spots had a N-terminal sequence identical to that of the 38 kDa glycolipoprotein (pstS-1). Other protein spots could not determine sequences. An antiserum against the recombinant 38 kDa antigen of M tuberculosis reacted strongly with the purified the 38 kDa antigen.
Ammonium Sulfate
;
Animals
;
Cell Wall
;
Chromatography, Affinity
;
DNA
;
Durapatite
;
Electrophoresis
;
Humans
;
Immune System
;
Isoelectric Focusing
;
Macrophages
;
Mice
;
Mycobacterium tuberculosis*
;
Mycobacterium*
;
T-Lymphocytes
;
Tuberculosis
9.Increased IL-12 and Interferon-Hamma, But Not IL-18 Production, After In Vitro Stimulation with a 30-kDa Mycobacterial Antigen in Patients with Tuberculous Pleurisy.
Chang Hwa SONG ; Eun Kyeong JO ; Hwa Jung KIM ; Jae Hyun LIM ; Un Ok KIM ; Tae Hyun PAIK ; Ji Won SUHR ; Jeong Kyu PARK
Journal of the Korean Society for Microbiology 2000;35(5):358-358
No Abstract Available.
Humans
;
Interleukin-12*
;
Interleukin-18*
;
Tuberculosis, Pleural*
10.Epidemiologic study of mumps in a part of large city.
Byung Hwa KEUM ; Jung Wan KWON ; Jong Han LIM ; Mi Kyung OH ; Sang Hwa LEE ; Youn Jin KIM ; Hye Ree LEE ; Bang Bu YOUN
Journal of the Korean Academy of Family Medicine 1991;12(8):65-71
No abstract available.
Epidemiologic Studies*
;
Mumps*