Estrogen may promote osteoblast/osteocyte viability by limiting apoptotic cell death. We hypothesize that hsp27 is an estrogen- regulated protein that can promote osteoblast viability by increasing osteoblast resistance to apoptosis. The purpose of this study was to determine the effect of estrogen treatment and heat shock on TNF alpha- induced apoptosis in the MC3T3-E1 cell line. Cells were treated with 0 - 100 nM 17betaestradiol (or ICI 182780) for 0 - 24 hours before heat shock. After recovery, apoptosis was induced by treatment with 0 - 10 ng/ml TNF alpha. Hsp levels were evaluated by Northern and Western analysis using hsp27, hsp47, hsp70c and hsp70i - specific reagents. Apoptosis was revealed by in situ labeling with Terminal Deoxyribonucleotide Transferase (TUNEL). A 5 - fold increase in hsp27 protein and mRNA was noted after 5 hours of treatment with 10 - 20 nM 17beta estradiol prior to heat shock. Increased abundance of hsp47, hsp70c or hsp70i was not observed. TUNEL indicated that estrogen treatment also reduced (50%) MC3T3-E1 cell susceptibility to TNF alpha-induced apoptosis. Treatment with hsp27-specific antisense oligonucleotides prevented hsp27 protein expression and abolished the protective effects of heat shock and estrogen treatment on TNF alpha-induced apoptosis. Hsp27 is a determinant of osteoblast apoptosis, and estrogen treatment increases hsp27 levels in cultured osteoblastic cells. Hsp27 contributes to the control of osteoblast apoptosis and may be manipulated by estrogenic or alternative pathways for the improvement of bone mass.
Apoptosis* ; Cell Death ; Cell Line ; Estradiol ; Estrogens* ; Hot Temperature ; HSP27 Heat-Shock Proteins ; In Situ Nick-End Labeling ; Indicators and Reagents ; Oligonucleotides, Antisense ; Osteoblasts* ; RNA, Messenger ; Shock ; Transferases ; Tumor Necrosis Factor-alpha

Odontogenic myxoma is one of rare tumors in oral and maxillofacial region and it is thought to be mesenchymal or ectomesenchymal origin. Its characteristics are benign and non-metastatic but it has the potential of local invasion and high recurrence rate. It originally occurs in atrium of heart and in central case, my xoma is located mainly in the maxilla and mandible. Most odontogenic myxoma develops in 2nd or 3rd decades of life and rarely occurs in child or older persons over fifty. The distribution of reported cases between the sexes is similar and the maxilla and mandible are equally affected or slightly higher in mandible. Clinically it is usually asymptomatic, however it can cause pain and paresthesia is complained in the advanced stages. Displacement and mobility of teeth have also been reported Odontogenic myxoma is not a frequent tumor, but in case of slow and painless growing tumor it must be considered as a differential diagnosis.
Child ; Diagnosis, Differential ; Heart ; Humans ; Jaw ; Mandible ; Maxilla ; Myxoma* ; Paresthesia ; Recurrence ; Tooth

This study was to analyze the changes of levels of alkaline phosphatase before and after enucleation of jaw cysts combined with bone grafting, and to evaluate biochemically the effectiveness of the early detection of bone healing and infection as a prognostic marker. Eighteen patients (13 males, 5 females) with cystic lesions of the jaws were divided into two groups. The bone graft group underwent enucleation and bone graft. The control group underwent only enucleation. Both groups were measured levels of ALP before surgery, and plus-minus 4 weeks postoperatively. The more discriminating results were obtained in the bone graft group. The results were as follows : 1. Levels of ALP after enucleation of jaw cysts were decreased in all patients with and without bone graft. 2. The bone graft group showed more marked decrease in variation of levels of ALP than the control group.(p=0.008) This should be considered as a result of increased osteoblastic activity and new bone formation. 3. Such variation could be used as a prognostic marker for bone healing after cyst operation. In the cost/benefit ratio, measurement of ALP activity could be useful as a convenient procedure in routine clinical practice.
Alkaline Phosphatase* ; Bone Transplantation ; Humans ; Jaw Cysts ; Jaw* ; Male ; Odontogenic Cysts ; Osteoblasts ; Osteogenesis ; Transplants

Animals ; Bone Regeneration ; Brain ; Connective Tissue ; Extracellular Matrix ; Fibronectins ; Humans ; Mesenchymal Stromal Cells ; Mice ; Mice, SCID ; Mice, Transgenic ; Models, Animal ; Neurons ; Osteoblasts ; Osteogenesis ; Publications ; Rats ; Stem Cells ; Tissue Donors

Alveolar Ridge Augmentation ; Animals ; Durapatite ; Factor VIII ; Fibrin Tissue Adhesive ; Fibrin ; Fibrinogen ; Hemostasis ; Humans ; Polymers ; Rats ; Skin ; Subdural Space ; Surgery, Oral ; Sutures ; Thrombin ; Tissue Adhesives ; Tooth Extraction ; Transplants ; Wound Healing