Compounds from Lingzhi has been demonstrated the ability for inhibiting tyrosinase (a key enzyme in melanogenesis) activity. In this study, we investigated the anti-melanogenic activity from the submerged mycelial culture of Ganoderma weberianum and elucidated the skin lightening mechanism by B16-F10 murine melanoma cells. From the cellular context, several fractionated mycelium samples exhibited anti-melanogenic activity by reducing more than 40% extracellular melanin content of B16-F10 melanoma cells. In particular, the fractionated chloroform extract (CF-F3) inhibited both secreted and intracellular melanin with the lowest dosage (25 ppm). Further analysis demonstrated that CF-F3 inhibited cellular tyrosinase activity without altering its protein expression. Taken together, our study has demonstrated that the chemical extracts from submerged mycelial culture of G. weberianum have the potential to serve as an alternative anti-melanogenic agent.
Chloroform ; Ganoderma ; Melanins ; Melanoma ; Monophenol Monooxygenase ; Mycelium ; Reishi ; Skin

BACKGROUND: Facial flushing response to drinking is observed in East Asians with deficient activity of the variant aldehyde dehydrogenase 2 genotype. This study examined the role of flushing response in the relationship between alcohol consumption and dyslipidemia. METHODS: This cross-sectional study included 1,443 Korean men, including 261 non-drinkers, 470 flushers and 712 non-flushers. Based on a questionnaire, weekly alcohol consumption was assessed and categorized into 4 groups. Dyslipidemia was defined by the National Cholesterol Education Program-Adult Treatment Panel III criteria. After adjusting for confounding factors, we evaluated the relationship between weekly alcoholic drinks and dyslipidemia in flushers and non-flushers by comparing with non-drinkers, using a multi-variable logistic regression analysis. RESULTS: Non-flushers had a significant relationship between hypertriglyceridemia and alcohol consumption in two groups (4-8 drinks: adjusted odds ratio [aOR] 1.937, 95% confidence interval [CI] 1.029-3.644; ≥16 drinks: aOR 2.118, 95% CI 1.272-3.527) in contrast to flushers, who showed no significant relationship between hypertriglyceridemia and alcohol consumption. Non-flushers had a significant relationship to low serum high density lipoprotein (HDL) cholesterol levels in four groups (<4 drinks: aOR 0.428, 95% CI 0.277-0.662; 4-8 drinks: aOR 0.409, 95% CI 0.216-0.774; 8-16 drinks: aOR 0.285, 95% CI 0.152-0.536; ≥16 drinks: aOR 0.343, 95% CI 0.207-0.568), and flushers had a significant relationship in two groups (8-16 drinks: aOR 0.234, 95% CI 0.102-0.536; ≥16 drinks: aOR 0.342, 95% CI 0.166-0.705). CONCLUSIONS: Our results suggest that drinking alcohol increases the risk of hypertriglyceridemia in non-flushers and the risk of low HDL cholesterol flushers.
Alcohol Drinking* ; Alcoholics ; Aldehyde Dehydrogenase ; Asian Continental Ancestry Group ; Cholesterol ; Cholesterol, HDL ; Cross-Sectional Studies ; Drinking ; Dyslipidemias* ; Education ; Flushing* ; Genotype ; Humans ; Hypertriglyceridemia ; Lipoproteins ; Logistic Models ; Male* ; Odds Ratio ; Triglycerides

BACKGROUND: The Alcohol Use Disorder Identification Test (AUDIT) has been widely used to identify alcohol use disorder (AUD). This study evaluated the validity of the AUDIT-Korean revised version (AUDIT-KR) for screening AUD according to Diagnostic and Statistical Manual of Mental Disorders, fifth edition (DSM-5) criteria. METHODS: This research was conducted with 443 subjects who visited the Chungnam National University Hospital for a comprehensive medical examination. All subjects completed the demographic questionnaire and AUDIT-KR without assistance. Subjects were divided into two groups according to DSM-5 criteria: an AUD group, which included patients that fit the criteria for AUD (120 males and 21 females), and a non-AUD group, which included 146 males and 156 females that did not meet AUD criteria. The appropriate cut-off values, sensitivity, specificity, and positive and negative predictive values of the AUDIT-KR were evaluated. RESULTS: The mean±standard deviation AUDIT-KR scores were 10.32±7.48 points in males and 3.23±4.42 points in females. The area under the receiver operating characteristic curve (95% confidence interval, CI) of the AUDIT-KR for identifying AUD was 0.884 (0.840–0.920) in males and 0.962 (0.923–0.985) in females. The optimal cut-off value of the AUDIT-KR was 10 points for males (sensitivity, 81.90%; specificity, 81.33%; positive predictive value, 77.2%; negative predictive value, 85.3%) and 5 points for females (sensitivity, 100.00%; specificity, 88.54%; positive predictive value, 52.6%; negative predictive value, 100.0%). CONCLUSION: The AUDIT-KR has high reliability and validity for identifying AUD according to DSM-5 criteria.
Alcohol Drinking ; Chungcheongnam-do ; Diagnostic and Statistical Manual of Mental Disorders* ; Drinking ; Female ; Humans ; Male ; Mass Screening* ; Reproducibility of Results ; ROC Curve ; Sensitivity and Specificity ; Surveys and Questionnaires

BACKGROUND: The Alcohol Use Disorder Identification Test (AUDIT) has been widely used to identify alcohol use disorder (AUD). This study evaluated the validity of the AUDIT-Korean revised version (AUDIT-KR) for screening AUD according to Diagnostic and Statistical Manual of Mental Disorders, fifth edition (DSM-5) criteria. METHODS: This research was conducted with 443 subjects who visited the Chungnam National University Hospital for a comprehensive medical examination. All subjects completed the demographic questionnaire and AUDIT-KR without assistance. Subjects were divided into two groups according to DSM-5 criteria: an AUD group, which included patients that fit the criteria for AUD (120 males and 21 females), and a non-AUD group, which included 146 males and 156 females that did not meet AUD criteria. The appropriate cut-off values, sensitivity, specificity, and positive and negative predictive values of the AUDIT-KR were evaluated. RESULTS: The mean±standard deviation AUDIT-KR scores were 10.32±7.48 points in males and 3.23±4.42 points in females. The area under the receiver operating characteristic curve (95% confidence interval, CI) of the AUDIT-KR for identifying AUD was 0.884 (0.840–0.920) in males and 0.962 (0.923–0.985) in females. The optimal cut-off value of the AUDIT-KR was 10 points for males (sensitivity, 81.90%; specificity, 81.33%; positive predictive value, 77.2%; negative predictive value, 85.3%) and 5 points for females (sensitivity, 100.00%; specificity, 88.54%; positive predictive value, 52.6%; negative predictive value, 100.0%). CONCLUSION: The AUDIT-KR has high reliability and validity for identifying AUD according to DSM-5 criteria.
Alcohol Drinking ; Chungcheongnam-do ; Diagnostic and Statistical Manual of Mental Disorders* ; Drinking ; Female ; Humans ; Male ; Mass Screening* ; Reproducibility of Results ; ROC Curve ; Sensitivity and Specificity ; Surveys and Questionnaires

No abstract available.
Mass Screening ; Alcohol Drinking ; Alcohol-Related Disorders Alcohols ; Alcohols ; Diagnostic and Statistical Manual of Mental Disorders ; Reproducibility of Results ; Sensitivity and Specificity