PURPOSE: Telomerase is known to play a role of adding repetitive parts to chromosomal ending and to be involved in carcinogenic process through cell immortalization. The purpose of this study is to evaluate that restraining of telomerase activation can have killing effect on cancer cell and enhance susceptibility of cancer cells to anticancer substance. METHODS: The killing effect on melanoma cells was studied by using recombinant adenovirus that makes it possible to inhibit telomerase from getting activated, with such targets as two types of melanoma cell lines. This recombinant adenovirus was used combined with cisplatin, one of the most representative anticancer medicine to evaluate enhancement in susceptibility of cancer cells to anticancer substance. RESULTS: From the result of cytotoxic assay, it is found that melanoma cells have much resistance to cisplatin on the whole. In the case of using Ad-OA of recombinant virus alone, killing effect on melanoma cells was insignificant. On the other hand, when Ad-OA was used in combination with cisplatin, susceptibility of melanoma cell lines to cisplatin was enhanced. CONCLUSIONS: Ad-OA, recombinant adenovirus, could be used as a supplementary medicine in the targeted cancer gene therapy against cancer cell lines resistant to cisplatin.
Adenoviridae* ; Cell Line* ; Cisplatin* ; Genes, Neoplasm ; Hand ; Homicide ; Melanoma* ; Telomerase

A nursing activity classification for hospitalized patients was performed based on an article review regarding nursing definition and nursing activity classification system. The study was conducted as follows: 1) Taxonomy was developed by the research team through the Delphi process and review article. The taxonomy consists of four nursing processes, (assessment, diagnosis, intervention and evaluation) and twelve nursing activity domains space (resperation, nutrition, elimination, exercise/alignment maintenance, comfort, hygiene, safety, spiritual support, counseling/ education, medication, communication, patient and information management). 2) First, nursing activities of the intervention process were listed and then classified by the nursing process of assessment, diagnosis, intervention and evaluation. The list consists of twelve nursing activity domains and 136 nursing activities. 3) A pilot study was conducted in two hospitals to verify validity and appropriateness of nursing activities. 4) The content validity index, which was calculated by 6 clinical practice experts, was 0.95. Also, a nursing activity classification system should also be developed in the department of community nursing and home health care nursing.
Classification* ; Diagnosis ; Education ; Home Health Nursing ; Humans ; Hygiene ; Korea* ; Nursing Process ; Nursing* ; Pilot Projects

No abstract available.
Bacteria, Anaerobic*

PURPOSE: The purpose of this study was to identify the effects of structured information provided on self care knowledge, self care performance, and functional status. METHOD: The data were collected using self care knowledge and performance assessment tool, and functional status assessment tool from both group hospitalized patients at D hospital in Busan. RESULTS: There was a significant improvement(p=.032) in self care knowledge in experimental group compared to the control group. But there were not improvement in self care performance and functional status in experimental group compared to the control group. But comparing to pretest and posttest in experimental group, There were significant improvement in self care performance (p=.003) and functional status(p=.013). CONCLUSION: Structured information provided showed increased in the degree of knowledge, self care performance, and functional status. But the effect size of program which had been developed in this study was estimated small, so there is needed to modify this program and to research repeatedly.
Busan ; Humans ; Liver Cirrhosis* ; Liver* ; Self Care*

Vulvar lichen sclerosus (LS) is a chronic inflammatory dermatosis and is considered a precursor lesion of squamous cell carcinoma (SCC); with about 3% to 5% of cases progressing to SCC, even up to 7%. LS can be found in adjacent areas in more than 60% of cases of vulvar SCC. As a chronic scarring inflammatory dermatosis, vulvar LS can act as both an initiator and promoter of carcinogenesis. All suspicious lesions should be biopsied. Although the association between LS and SCC has been reported in the literature, actual case reports are rare in South Korea.

Low-grade fibromyxoid sarcoma (LGFMS) is a rare malignant tumor that typically manifests as a painless, slow-growing mass in the deep soft tissues of the proximal extremities or trunk. While commonly occurring in young adults, it rarely presents in older individuals. LGFMS is characterized by seemingly benign histological features and a paradoxically aggressive clinical course. LGFMS often exhibits alternating fibrous and myxoid areas arranged in a spiral pattern, featuring benign fibroblast spindle cells. Despite its low-grade appearance, LGFMS has the potential for late metastasis. Diagnosing LFGMS can be challenging owing to its indolent growth pattern and benign appearance on small biopsy specimens, which may lead to confusion with benign soft-tissue tumors.Differential diagnosis of myxofibrosarcoma and myxoid neurofibromas is required. Surgical excision with wide margins is recommended for LGFMS management. We report a typical case of LGFMS located on the right inner thigh of an 82-year-old male.

We compared antiobese, hypocholesterolemic, antiplatelet and antioxidant effect of 10% green tea powder and 3% green tea extract in rats pair fed 5% cholesterol diets. The final body weight was decreased significantly compared with the control (p < 0.05). Plasma and liver total cholesterol were lower in group of green tea powder or extract, but not statistically different. HDL cholesterol was increased significantly in group of green tea powder compared with the control or green tea extract (p < 0.05). Plasma triglyceride was significantly decreased in group of green tea extract compared with green tea powder, and green tea powder compared with the control respectively (p < 0.05). Liver triglyceride was significantly decreased in group of green tea powder or green tea extract compared with the control (p < 0.01). Platelet aggregations in the maximum and initial slope were not different among groups. Hemolysis was significantly lower in group of green tea powder compared with the control (p < 0.05). Plasma TBARS production was decreased in group of green tea extract compared with the control (p < 0.05). Na passive leak in intact cells was not different, but Na leak in AAPH treated cell was significantly decreased in group of green tea powder than the control (p < 0.05). The leak increase (delta Na Leak) after AAPH treatment was significantly decreased in groups of green tea powder and extract compared with the control (p < 0.05). Isotope excretion after 14C-cholesterol ingestion was significantly increased in group of green tea extract compared with the control or the green tea powder (p < 0.05). Consumption of green tea in powder or extract may give beneficial effects in weight control and plasma lipid profiles, impeding metabolic syndrome. More studies are needed to clarify what component of green tea and what mechanism are involved in antiobese and hypolipedemic actions of green tea.
Amidines ; Animals ; Antioxidants ; Blood Platelets ; Body Weight ; Cholesterol ; Cholesterol, HDL ; Diet ; Eating ; Hemolysis ; Lipid Peroxidation ; Liver ; Plasma ; Rats ; Tea ; Thiobarbituric Acid Reactive Substances ; Weight Gain

OBJECTIVE: The purpose of this study was to identify differences in measurement methods for blood pressure (Direct measurement, HP automatic indirect measurement, and SE 7000 Korean made indirect measurement), and to evaluate the clinical trial of the Korean made automatic indirect blood pressure measurement. MATERIAL & METHODS: From June, 1999 to February, 2000, fifty five consecutive patients were randomized into hypertension group (n=20), normotension group (n=20), and hypotension group (n=15). Measuring blood pressure by indirect methods (HP NIBP M 1008B and SE 7000 NIBP Korean made) was done simultaneously in the same arm with arterial line for direct blood pressure measurement (HP M1006A). The procedures were repeated ten times at intervals of 2 minutes. Statistical analysis was performed using SPSS (version 8.0 for windows) software package. Values were expressed as means and standard deviation and means were compared using t-test. Statistical significance was considered present with a p value less than 0.05. RESULTS: In the hypertension group and noromotension group, the disparity between HP direct measurement and indirect SE 7000 NIBP did not show any differences compared to the disparity between HP direct measurement and indirect HP NIBP. In the hypotension group, the disparity in the diastolic pressure between HP direct measurement and indirect SE 7000 NIBP was significantly different compared to the disparity between HP direct measurement and indirect HP NIBP (p<0.001), however, disparities in systolic pressure did not showed any differences. CONCLUSION: Direct blood pressure measurement (HP M1006A) can be replaced with indirect blood pressure measurements (HP NIBP M 1008B & SE 7000 NIBP) in normotension and hypertension patients. Korean made indirect measurement was found to be more accurate compared to HP indirect measurement in hypotension patients, but more study is needed.
Arm ; Blood Pressure* ; Humans ; Hypertension ; Hypotension ; Vascular Access Devices

The purpose of the study is to develop a Web-based health information service system for the middle-aged women. To identify users' information needs, we conducted an online survey of 189 middle-aged women. It was found that they had various health problems but very few did any health prevention or health promotion activities. We developed following contents in the following areas based on the result of needs assessment. They were exercise, diet including weight control, osteoporosis, climacteric, aging, breast and cervical cancer. Besides this health information, FAQ on women's health, my health knowledge test, statistics, link to related institutes and web sites, newspaper articles/mass media clips, journal articles, conferences papers, and other educational information were added. The homepage was developed as a subsystem of the Healthguide. It was evaluated by the users and the expert panel. The users rated 3.6 out of 5 point in their satisfaction rating and the experts rated 3.9 out of 5 in their site evaluation. It is expected that a Web-based women's health information service system will contribute to women's health promotion as well as provide a community for women's health related researchers and experts to share information on women's health.
Academies and Institutes ; Aging ; Breast ; Climacteric ; Congresses as Topic ; Diet ; Female ; Health Promotion ; Humans ; Information Services* ; Internet ; Middle Aged ; Needs Assessment ; Periodicals ; Osteoporosis ; Uterine Cervical Neoplasms ; Women's Health*

The purpose of the study was to assess customer satisfaction concerning service quality characteristics of university foodservice by using a developed DINESERV model. In particular, it was intended to develop a tool to assess the difference between customer judgements on importance and customers perceptions with actual service delivery by university foodservices. Quenstionnaires were distributed to 1,000 university students. A total at 820 university students responded with a usable response rate of 77.7%. A statistical data analysis was completed using SAS programs for descriptive analysis; a t-test, chi-square test and Dunan's multiple range test. The results of the study are as follows; 1) The mean number of students visiting university foodservices per week for males was larger than that of females. The students' first choice depended on distance when they selected foodservices. They answered their preference as the first factor when they order a particular menu items in foodservices. The first complaint factor concerning university foodservices was the price of the food. 2) Customers was not satisfied with the quality of the service of university foodservices. The important mean score of the service quality was 3.63 out of 5, but the perception mean score of the service quality was 2.87. Therefore, there was a gap(0.76) between the importance score and perception score. 3) Customers' satisfaction with the service quality by dimensions wee int he follow order: assurance>reliability>responsiveness>tangibles>empathy. Customers were more satisfied with the service quality of contracted management than that of self-operated facilities.
Data Interpretation, Statistical ; Female ; Humans ; Male