OBJECTIVE: This study was to determine the effect of different concentration of calcium in medium on the preimplantational development of zygotes and early 2-cell embryos. METHODS: Female mice of ICR strain (5~8 weeks old) were superovulated and mated with fertile males. Zygotes or early 2-cell embryos were collected by flushing the oviducts 31~32 hours after hCG injection. The embryos were cultured in various concentrations of Ca2+ in medium or with EDTA, EGTA and Ni2+. RESULT AND CONCLUSION: Treatment of high concentration of Ca2+ (3.42 mM (2X)~17.1 mM (10X) in medium didn't develop well compared to the control Low concentrations of Ca2+ (0.214 mM (1/8X)~0.855 mM (1/2X)) were deterimental to development beyond 2-cell stage. EDTA, Ca2+ chelating agent was treated with ranged concentrations of eDTA (0.014 mM~0.107 mM) to medium contaning 1.71 mM Ca2+ showed beneficial effect to development to blastocyst compared to the control. EGTA, extracellular Ca2+ chelator, was treated with ranged concentrations of EGTA (0.014~0.107 mM) to the medium contaning 1.71 mM Ca2+. There is no significant difference with the control. Ni2+ (50 micrometer), T-type Ca2+-channel blocker was treated to medium contaning low concentration of Ca2+. It overcame 2-cell block significantly. Rate of degenerated embryos decreased and developmental rate to morula and blastocyst increased more than low Ca2+ concentration alone. Further studies are needed for the overcoming effect of 2-cell block by Ni2+.
Animals ; Blastocyst ; Calcium ; Edetic Acid ; Egtazic Acid ; Embryonic Structures* ; Female ; Flushing ; Humans ; Male ; Mice* ; Morula ; Oviducts ; Zygote

OBJECTIVE: The study was performed to compare the survival rate and the development of day 2 mouse embryos which had freezing procedures done. METHODS: We used three different vitrification solutions (EFS, VS14, DPS) and a ultrarapid freezing solution (UFS) for cryopreservation of day 2 mouse embryo. RESULTS: We tested toxicity by exposing embryos to vitrification solutions and a ultrarapid freezing solution. The survival rates are 100%, 97.8%, 95.6% and 100% (EFS, VS14, DPS and UFS). After cultured for 96 hours, hatching rates of each group are 93.5% (no freezing), 95.6% (EFS), 86.4% (VS14), 93.0% (DPS), and 93.0% (UFS). There is no significant differences among groups. The survival rates after thawing cryopreserved embryos are 80.2%, 91.7%, 69.5%, 0% and 91.8% (slow freezing, EFS, VS14, DPS and UFS). Also cultured for 96 hours, the hatching rates are 93.5% (no freezing), 84.1% (slow freezing), 93.9%) (EFS), 48.5% (VS14) and 70.1% (UFS). CONCLUSION:The survival rates of vitrification in EFS solution and ultrarapid freezing are higher than slow freezing (p<0.05). The hatching rate of vitrification in EFS solution cultured for 96 hours is highest, so vitrification of day 2 mouse embryos in EFS solution considered as more effective for cryopreservation.
Animals ; Cryopreservation ; Embryonic Structures* ; Freezing* ; Mice* ; Survival Rate ; Vitrification*

Extramammary Paget's disease is a dermatosis which pathologically and clinically resembles Paget's disease of the breast. The disease occurs in regions with apocrine gIands; most often in the anogenital area, seldom in the axilla. umbilicus, mucous membrane of the mouth, nose, ear and Moll's gland of tne eyelid. In this work the effect of bleomycin for extramammary Paget's disease was studied on ultrastructural aspect. The material was a case of extramammary Paget's disease of 7 years standing occuring in the scrotum and the pubic area by serial sections after IV injection of 60mg, 120mg and 240mg of bleomycin, and examined under the electron microscope. The results obtained are summarized as follows: 1. The size of Paget's cell and its nucleus was gradually decreased. 2. The numbers of lysosome, dense body, autophagic vacuolization and tonofilament was decreased. From these fidings, it is suggested that bleomycin induces retrograde degenerative change of Pagct's cell, and cell necrosis finally.
Axilla ; Bleomycin* ; Ear ; Eyelids ; Intermediate Filaments ; Lysosomes ; Mouth ; Mucous Membrane ; Necrosis ; Nose ; Paget Disease, Extramammary ; Paget's Disease, Mammary ; Scrotum ; Skin Diseases ; Umbilicus

No abstract available.
Carcinogenesis* ; Oenothera biennis*

A recently recognized connective tissue disorder, Loeys-Dietz syndrome (LDS) is a genetic aortic aneurysm syndrome caused by mutations in the transforming growth factor-receptor type I or II gene (TGFBR1 or TGFBR2). They have distinctive phenotypic abnormalities including widely spaced eyes (hypertelorism), bifid uvula or cleft palate, and arterial tortuosity with aortic aneurysm or dissection throughout the arterial tree. LDS is characterized by aggressive and rapid progression of aortic aneurysm. Therefore, the patients with distinct phenotype, marked aortic dilatation and aneurysm at early age should be suspected to be affected by LDS and rapid TGFBR gene analysis should be done. We report one child diagnosed as LDS due to typical phenotypes and two novel missense mutations of the TGFBR2 gene (c.1526G>T and c.1528A>T).
Aneurysm ; Aortic Aneurysm ; Arteries ; Child ; Cleft Palate ; Connective Tissue ; Dilatation ; Eye ; Humans ; Joint Instability ; Loeys-Dietz Syndrome ; Mutation, Missense ; Phenotype ; Protein-Serine-Threonine Kinases ; Receptors, Transforming Growth Factor beta ; Skin Diseases, Genetic ; Thorax ; Uvula ; Vascular Malformations

PURPOSE: The purpose of this study was to determine the factors that affect suicidal ideation in patients with chronic mental illnesses, such as schizophrenia, depressive disorder, and bipolar disorder. METHODS: The research participants were 160 patients with mental illnesses residing in B City and G Province. They responded to questionnaires about their suicidal ideation, self-esteem, depression, family support, social support, and internalized stigma of mental illness. The collected data were analyzed using t-test, ANOVA, Pearson correlation coefficients, and STEPWISE multiple regression with the SPSS/WIN 18.0 program. RESULTS: History of self-esteem, Depression, Social support, Psychiatric diagnosis of MDD and suicide attempt showed an explanatory power for 64% of suicidal ideation in patients with chronic mental illnesses. CONCLUSION: Specialized health and medical service personnel providing services to patients with chronic mental illnesses must determine the risk factors and protective factors for suicidal ideation associated with each illness, and establish a more individualized and systemized case management system based on customized approach.
Bipolar Disorder* ; Case Management ; Depression ; Depressive Disorder* ; Depressive Disorder, Major ; Humans ; Mental Disorders ; Risk Factors ; Schizophrenia* ; Suicidal Ideation* ; Suicide

No abstract available.
Interleukin-6* ; Rabbits*

Hemiballism-hemichorea is a rare movement disorder that presents unilateral flinging movements of the limbs. Recent studies pointed non-ketotic hyperglycemia as an underlying cause of this movement disorder. We report an elderly women with irregular, involuntary movement of the limbs after her uncontrolled diabetes mellitus was successfully managed by the control of glucose level. Characteristic magnetic resonance imaging findings and the management of this condition were discussed.
Aged ; Diabetes Mellitus ; Dyskinesias ; Extremities ; Female ; Glucose ; Humans ; Hyperglycemia ; Magnetic Resonance Imaging ; Movement Disorders*

PURPOSE: The purpose of this study was to identify sleep disorder experience in older patients living in the community and acknowledging being depressed. METHODS: For this study, 11 older patients with depression were purposively chosen. Data were collected through in-depth individual interviews from July 2013 to January 2014 and analyzed in terms of by Giorgi's phenomenological methodology. RESULTS: The study results showed that sleep disorder experience in older patients with depression consisted of sixteen themes and five themes-clusters: 1) exposure of the causes of sleep disorders; 2) life is painful; 3) harassing themselves and their family; 4) difficulty in controlling themselves; 5) trying to deal with the sleep disorder to overcome the situation. CONCLUSION: It is necessary to develop educational guidelines for patients with sleep disorders or nursing interventions that anyone can easily provide for elders in the communities. The first priority should be given to efforts to apply diverse methods to improve sleep hygiene and minimize the period of exposure to medication before starting medication for patients with sleep disorders.
Activities of Daily Living ; Adaptation, Psychological ; Aged ; Depression/complications/*diagnosis ; Family Relations ; Female ; Humans ; Interviews as Topic ; Male ; Sleep Disorders/complications/diagnosis/*psychology ; Stress, Psychological

A case or right occipital lobe infarction initially detected by the visual field examination and confirmed with the computerized tomography is presented. Reduction of significance of visual field test for identification of intracranial lesion as, the initial diagnostic procedure after the orientation of computerized tomography is unlikely. An occipital lobe lesion developed following posterior cerebral artery thrombosis has introd uced silgnificant ophthalmic symptom, namey, visual field defect, diplopia, ipsilateral ocular pain, visual hallucination, photophobia, and Anton's syndrom, etc. A characteristic feature of left homonymous hemianopsia with macular sparing due to right posterior cerebral artery thromb osis is demonstrated.
Diplopia ; Hallucinations ; Hemianopsia ; Infarction* ; Occipital Lobe* ; Photophobia ; Posterior Cerebral Artery ; Thrombosis ; Visual Field Tests ; Visual Fields