2.Immune responses of mucosal epithelial cells in response to pathogenic microbial infections.
Journal of Asthma, Allergy and Clinical Immunology 2000;20(1):68-74
No abstract available.
Epithelial Cells*
3.Expression of Proinflammatory Cytokine Genes in Mouse Peritoneal Tissue Infected with Bacteroides fragilis.
Yang Ja CHO ; Jung Mogg KIM ; Won Seop KIM
Journal of the Korean Society for Microbiology 1999;34(3):253-264
OBJECTIVE: Bacteroides fragilis, normal colonic inhabitant, is the most frequently isolated anaerobes in infected tissues, particularly in intraabdominal abscesses. In the acute infection model with abscesses, the response to B. fragilis infection is characterized by infiltration of neutrophils and macrophages. This study was designed to determine whether proinflammatory cytokines could be upregulated in peritoneal tissue of B. fragilis-infected mouse model. METHODS: After C57BL/6 mice were infected with abscess-inducing encapsulated B. fragilis, RNA was extracted from the intraperitoneal tissues. Cellular RNA was also extracted from mouse peritoneal macrophages (MPM) and human peripheral blood mononuclear cells (PBMC) after infection with B. fragilis. Expression of various cytokine mRNA was assessed using RT-PCR and standard RNA. Each cytokine protein was also measured by ELISA. RESULTS: B. fragilis-infected intraperitoneal tissues showed upregulated expression of IL-1u, IL-6 and TNFu mRNA. Expression of IL-1u and TNFu mRNA and protein was significantly higher in MPM or PBMC infected with B. fragilis than in those without infection. However, expression of IL-6 mRNA and protein was not increased in MPM or PBMC infected with B. fragilis compared with those without infection. CONCLUSION: These results suggest that the cytokines can be involved in immunopathologic reactions of the peritoneal tissue infected with B. jragilis.
Abscess
;
Animals
;
Bacteroides fragilis*
;
Bacteroides*
;
Colon
;
Cytokines
;
Enzyme-Linked Immunosorbent Assay
;
Humans
;
Interleukin-6
;
Macrophages
;
Macrophages, Peritoneal
;
Mice*
;
Neutrophils
;
RNA
;
RNA, Messenger
4.Effect of electric shock stress or psychological conflict stress on the proliferation and interleukin-2 productio of rat splenic lymphocyte.
Sang Jin HAN ; Byung Hwan YANG ; Jung Mogg KIM
Journal of Korean Neuropsychiatric Association 1992;31(2):208-220
No abstract available.
Animals
;
Interleukin-2*
;
Lymphocytes*
;
Rats*
;
Shock*
5.Expression of Cyclooxygenase - 2 in Intestinal Epithelial Cells in Response to Invasive Bacterial Infection and its Role of Epithelial Cell Apoptosis.
Jung Mogg KIM ; Shin Jae KANG ; Yang Ja CHO
Journal of the Korean Society for Microbiology 1999;34(5):479-489
Invasion of enteric bacteria, such as Salmonella and invasive E. coli, into intestinal epithelial cells induces proinflammatory gene responses and finally epithelial cell apoptosis. In this study, we asked whether invasive bacterial infection of human intestinal epithelial cells could upregulate cyclooxygenase-2 (COX-2) gene expression and whether increased COX-2 expression could influence intestinal epithelial cell apoptosis. Expression of COX-2 mRNA and prostaglandin (PG) E production were upregulated in HT-29 colon epithelial cells which were infected with S. dublin or invasive E. coli, as examined by quantitative RT-PCR and radioimmunoassay. Inhibition of COX-2 expression and PGE2 production using NS-398, a specific COX-2 inhibitor, showed a significant increase af epithelial cell apoptosis and caspase-3 activation in HT-29 cells infected with invasive bacteria. However, the addition of valerylsalicylate, a specific COX-1 inhibitor, did not change apoptosis in S. dublin-infected HT-29 cells. These results suggest that upregulated COX-2 expression and PGE2 production in response to invasive bacterial infection could contribute to host defense by inhibiting apoptosis of intestinal epithelial cells.
Apoptosis*
;
Bacteria
;
Bacterial Infections*
;
Caspase 3
;
Colon
;
Cyclooxygenase 2
;
Dinoprostone
;
Enterobacteriaceae
;
Epithelial Cells*
;
Gene Expression
;
HT29 Cells
;
Humans
;
Prostaglandin-Endoperoxide Synthases*
;
Radioimmunoassay
;
RNA, Messenger
;
Salmonella
6.Basolaterial Secretion of CXC Chemokines by Human Intestinal Epithelial Cells in Response to Bacteroides fragilis Enterotoxin Via NF-kB Pathway.
Jung Mogg KIM ; Yu Kyoung OH ; Young Jeon KIM ; Hee Bok OH ; Yang Ja CHO
Journal of the Korean Society for Microbiology 2000;35(5):350-350
No Abstract Available.
Bacteroides fragilis*
;
Bacteroides*
;
Chemokines, CXC*
;
Enterotoxins*
;
Epithelial Cells*
;
Humans*
;
NF-kappa B*
7.Basolaterial Secretion of CXC Chemokines by Human Intestinal Epithelial Cells in Response to Bacteroides fragilis Enterotoxin Via NF-kB Pathway.
Jung Mogg KIM ; Yu Kyoung OH ; Young Jeon KIM ; Hee Bok OH ; Yang Ja CHO
Journal of the Korean Society for Microbiology 2000;35(5):350-350
No Abstract Available.
Bacteroides fragilis*
;
Bacteroides*
;
Chemokines, CXC*
;
Enterotoxins*
;
Epithelial Cells*
;
Humans*
;
NF-kappa B*
8.Prophylactic effect of interleukin-2 on lethality by gram-negative bacteria in mice.
Hyung Su HAN ; Jung Mogg KIM ; Yong CHOI ; Wang Soo HAN ; Yang Ja CHO
Journal of the Korean Society for Microbiology 1991;26(4):345-353
No abstract available.
Animals
;
Gram-Negative Bacteria*
;
Interleukin-2*
;
Mice*
9.Antibiotic Resistance of Helicobacter pylori Isolated from Korean Patients.
The Korean Journal of Gastroenterology 2006;47(5):337-349
The distribution of minimal inhibitory concentrations (MIC) for amoxicillin, clarithromycin, metronidazole, tetracycline, azithromycin, and fluoroquinolone (ciprofloxacin, levofloxacin, and moxifloxacin) have shifted to higher concentrations from 1987 to 2003 in Helicobacter pylori (H. pylori) strains isolated from Korean patients. MIC values of secondary isolates were higher than those of primary isolates. Of treatment-failure patients, 16.4% showed mixed infections with both antibiotic-susceptible and -resistant H. pylori strains. A total of 89.6% of patients with treatment failure and 52.3% of patients without antibiotic treatment had H. pylori strains resistant to two or more antimicrobial agents (multi-drug resistance, MDR). The most common antibiotics showing MDR were clarithromycin, metronidazole, and azithromycin. The resistance rates to both amoxicillin and clarithromycin were 34.3% in secondary isolates and 6.2% in primary isolates. The resistance rates to both clarithromycin and metronidazole were 73.1% in secondary isolates and 7.7% in primary isolates. In addition, there was a significant difference in antibiotic resistance between two institutions located at Seoul and Gyeonggi provinces. To provide adequate informations about susceptible antibiotics to clinicians, continuous surveillance of antibiotic susceptibilities is needed in Korea.
*Drug Resistance, Bacterial
;
Drug Resistance, Multiple, Bacterial
;
Helicobacter pylori/*drug effects/isolation & purification
;
Humans
;
Korea
;
Microbial Sensitivity Tests
10.Effect of electric shock stress and psychological stress of conflict stimulation on interleukin-2 production and lymphokine-activatedkiller cell cytotoxicity.
Dong Rag CHOI ; Jung Mogg KIM ; Yong CHOI ; Yang Ja CHO
Korean Journal of Immunology 1991;13(2):151-162
No abstract available.
Interleukin-2*
;
Shock*
;
Stress, Psychological*