No abstract available.
Delivery of Health Care ; Informatics

GTP binding protein (G-protein) associated with membrane and involved in signal transduction was isolated from bovine brain, and molecular weight of G protein was observed. As the results, cell membranes were homogenized from bovine brain tissues and proteins of membrane were gained using 1% cholate, and progressed the chromatography. The purification process was performed by step, DEAE-Sephacel, Ulttrogel AcA 34 and heptylamine-Sepharose column chromatography. The chromatographic fractions were confirmed by GTP binding assay and SDS-polyacrylamide gel electrophoresis. Molecular weight of Goa was revealed 39,000 dalton and GR 36,000 dalton. One more step of heptylamine-Sepharose was enforced to purify the GTP binding protein. Finally I gained the GTP binding protein isolated subtype of Goalpha and Gbeta.
Brain* ; Cell Membrane ; Cholates ; Chromatography ; Electrophoresis ; GTP-Binding Proteins ; Guanosine Triphosphate* ; Membranes ; Molecular Weight ; Signal Transduction

No abstract available.
Drug Resistance, Multiple* ; Drug Therapy*

BACKGROUND: The cuff inflation of laryngeal mask airway (LMA) with excessive volume of air may injure soft tissue and nerves around hypopharynx because of high cuff pressure. But it is not well known about adequate cuff volume of LMA. The cuff inflation of LMA in hypopharynx accomodate pharynx, so various injection volume may differ in sealing effect. This may influence ventilation. This study was proposed to investigate the effect of cuff volume on cuff pressure and ventilation when LMA inserted. METHODS: Fifteen male patients were inserted with #4 LMA, fifteen female patients were inserted #3 LMA. For male patients, 10, 20, 30 and 40 ml of air were injected into cuff of LMA and 10, 15, 20 and 25 ml for female patients. Cuff pressure, minute ventilation and airway pressure were randomly measured. RESULTS: Volume of injected into cuff and cuff pressure of LMA were closly related. But minute ventilation and airway pressure were not related with injected air volume of LMA. CONCLUSION: Excessive volume of air injected to LMA cuff was not beneficial to ventilation. So minimal sealing cuff volume under maximal recommended volume of LMA may reduce the incidence of complications associated with high cuff pressure.
Female ; Humans ; Hypopharynx ; Incidence ; Inflation, Economic ; Laryngeal Masks* ; Male ; Pharynx ; Ventilation*

No abstract available.
Diagnosis* ; Diuretics ; Edema*

No abstract available.
Animals* ; Clone Cells*

No abstract available.

No abstract available.

Clonorchiasis for which no specific therapy is available has been recognized as one of the serious public health problems in terms of waste of human productivity in Korea today and an urgent consideration must be given to its prevention and control. For past several years, the author has been finding that the infection rate of liver fluke in fish from the Kum-Ho river which has long been recognized as the world's notorious prevalent area of the disease, has been decreasing gradually. Here the author formulated the hypothesis that the decreasing of infection rate might be influenced by the common use of pesticide in plants along the river. Basing this hypothesis, the effective use of pesticide may contribute to the control of the disease by cutting the life cycle of liver fluke. To test above hypothesis, molluscicidal effect on Parafossarulus manchouricus and cercariacidal effect of various pesticides in liver fluke have been determined and the following results were obtained. In molluscicidal effect, the most potent agent in low concentration was Bayer 73 which is 3 to 70 times stronger than other pesticides. When compared with NaPCP which has been widely used for the eradication of Onchomelania, Bayer 73 shows 5 to 10 times stronger effect. The order of pesticide in molluscicidal effect was: Bayer 73, Nicotin, NaPCP, Sevin, Parathion, Copper sulfate and Lindane. In cercariacidal effect, the most potent agent was Parathion which had 2 to 5 times stronger effect than Bayer 73 or NaPCP. The order of pesticide in the cercariacidal effect was: Parathion, Bayer73, NaPCP, Lindane, Nicotin,Sevin, and Copper sulfate. By applying the common used concentration of pesticides considering the toxicity to plants, Bayer 73 is most potent in molluscicidal effect and then Nicotin, NaPCP, Copper sulfate, Sevin, Parathion and Lindane in order. In cercariacidal effect, Parathion is the most potent and NaPCP, Bayer 73, Lindane, and Copper sulfate are followed. Considering with the toxicity to human body the most safe and effective pesticides were Bayer 73, Seven, NaPCP, and Copper sulfate but among those agents, Bayer 73 and NaPCP show relatively stronger effect in both Parafossarulus manchouricus and cercaria of liver fluke.
parasitology ; helminth ; trematode ; Clonorhcis sinensis ; chemotherapy ; Parafossarulus manchouricus ; Onchomelania ; cercaria ; toxicology ; Bayer 73 ; Nicotin ; NaPCP ; Sevin ; Parathion ; Copper sulfate ; Lindane

No abstract available.
Child* ; Humans