In the present study, we examined to determine the development of various lymphoid tissue including mucosa-associated lymphoid tissue (MALT), thymus, lymph node and liver. In order to investigate the relationship between the morphological events and the expression pattern of MIC2 antigen (CD99) during the development of lymphoid system, we performed the immunohistochemical study using DN16, a monoclonal antibody against MIC2 (CD99), on formalin-fixed and paraffin-embedded lymphoid sections in 68 human embryos and fetuses, between 5 and 39 gestational week (GW). Four neonates, an infant, and 5 adults are also included. CD99 has been expressed along the membrane of hepatocytes and sinusoidal endothelial cells for 10-28 GW, in when the liver the major site of hematopoiesis. In the thymus, CD99 was firstly detected in the presumptive epitheial cells at 10 GW. When the thymus matured and corticomedullary differentiation appeared, CD99 was exclusively expressed in cortical thymocytes. The CD99 expression in epithelial cells of MALT has initiated at 6 GW and 10 GW earlier than that at the onset of MALT development and its expression has been persisted during MALT formation especially 16-25 GW. The finnding that CD99 antigen was expressed in epithelial cells during the development of MALT rnight provide a means to identify a novel epithelial differentiated substance. In addition, endothelial cells that are present in various organs such as liver and small intestine concurrently expressed CD99 antigen and its expression persisted to late fetal period. This point rnight suggest that CD99 antigen regulate the irnigration of lymphocytes from liver, major hematopoietic organ, to thymus or peripheral lyrnphoid organ via the interaction between endothelial cells and lymphocytes.
Adult ; Embryonic Structures* ; Endothelial Cells ; Epithelial Cells ; Fetus* ; Hematopoiesis ; Hepatocytes ; Humans* ; Infant ; Infant, Newborn ; Intestine, Small ; Liver ; Lymph Nodes ; Lymphocytes ; Lymphoid Tissue* ; Membranes ; Thymocytes ; Thymus Gland

No abstract available.
Hospice Care ; Hospices

The method of treatment of acromioclavicular dislocation is controversial, and conservative and surgical treatment have been reported to be both successful. Recently, many surgeons tend to treat the acromioclavicular dislocation with anatomical reduction of acromioclavicular joint and rigid internal fixation because of many disadvantages of conservative methods. We report 24 cases of acute acromioclavicular dislocation treated with modified Phemister method from March 1989 to December 1992. The follow-up ranged from 12 months to 31 months with 16.5 months on average. The results are as follows. l. Among 24 cases, 21 cases are males and 20 cases are in 3rd decade to 5th decade. 2. The most common cause of injuries is falling down, followed by traffic accident. 3. Associated injuries are fractures in 5 cases, spleen rupture in 1 case. 4. Except 1 case with spleen rupture, 23 cases were treated within 2 weeks after injury. 5. Preoperative difference in C-C distance on stress view is 8.6mm on average, ranged from 6mm to 18mm. 6. Clinical results were excellent in 11 cases, good in 10 cases, fair in 2 case, and poor in 1 case. 7. Complications were pin migration in 1 case and symptomatic acromioclavicular arthritis in 1 case.
Accidental Falls ; Accidents, Traffic ; Acromioclavicular Joint ; Arthritis ; Dislocations ; Follow-Up Studies ; Humans ; Male ; Methods ; Rupture ; Spleen ; Surgeons

Fractures of the neck of the femur in children are so rare that none has a great experience of them. Therefore the literature is scanty and no detailed study of a large series of cases can be found. Generally there were several differens between femoral neck fracture of adult and child. Those are rare incidence, the mechanism of fracture, different blood supply of femoral head, difficulty discision of definitive treatment and high incidence of complications. In this report, we presented 4 cases. One of them was the dispiaced fracture and was treated with conservative method. The other 3 cases were the displaced fractures and were treated with operative method. Relatively good results were obtained.
Adult ; Child ; Femoral Neck Fractures ; Femur ; Femur Neck ; Head ; Humans ; Incidence ; Methods ; Neck

We analyzed the clinical and laboratory features of ten children with acute megakaryoblastic lukemia (M7)and compared the findings with those reported in the literature. The diagnosis was supprted by ultrastructural examination of platelet peroxidase or immunophenotyping for glycoprotein IIb/IIIa. Of the ten children, five were girls and five were boys. The median age at diagnosis was 13 months. Two patients had prominent myelofibrosis and one patient had Down syndrome. Nine patients were treatd with low-dose cytosine arabinoside (10mg/m2)administered intravenously, or subcutaneously, or intramuscularly, twice daily in 21 day courses. Seven patients achieved hematologic response and three patients are alive without evidence of disease. The 4 year event free survival rate was30.0%. It is our impression that the prevalence of acute megakaryoblastic leukemia has been under-estimated, and low-dose cytosine arabinoside treatment may be of value in its management. This approach may be particularily useful in hospitals with scarce well-equipped facilities, since this protocol does not induce profound marrow hypoplasia and intensive supportive measures are not required as they would be with the use of more aggressive drug combination.
Blood Platelets ; Bone Marrow ; Child* ; Cytarabine ; Diagnosis ; Disease-Free Survival ; Down Syndrome ; Female ; Glycoproteins ; Humans ; Immunophenotyping ; Leukemia, Megakaryoblastic, Acute* ; Megakaryocyte Progenitor Cells ; Peroxidase ; Prevalence ; Primary Myelofibrosis

No abstract available.

No abstract available.
Emergencies* ; Hematoma, Subdural, Acute* ; Trephining*

Here, we show that the interaction between two membrane proteins, the mouse homologue of CD99 (designated D4) and its ligand, paired immunoglobulin-like type 2 receptor (PILR), is one of the major mechanisms of thymocyte apoptosis. Using the polymeric fusion protein of PILR and IgG1 (PILR-Ig), we demonstrated that D4 ligation in the absence of T cell receptor (TCR) engagement leads to the induction of apoptosis, mainly at the double-positive stage of thymocytes. This was further confirmed by a blocking study in which blocking the interaction between D4 and PILR by soluble D4 protein led to reduced apoptosis in the fetal thymic organ culture with wild type and TCRalpha(-/-) mice. Furthermore, the dissection of intracellular signaling pathway demonstrated that D4 cross-linking led to caspase activation without any change in mitochondrial membrane potential. Based on these data, we propose a mechanism for thymocyte depletion in which the interaction between D4 and PILR delivers an active signal.

A totally implanted venous access system was inserted in 19 children with cancer. The devices were utilized for the administration of antineoplastic drugs, parenteral fluids, antibiotics, and blood products. Total duration of implantation was 4,046 days for 23 implanted system (range 7-445 days). Complications included cather infection (0.247/100 catheter days), occlusion (0.692/100 catheter days), and dislodgement of needle (0.643/100 catheter days). There were major complications that necessitated removal of catheters, including systemic infections (0.09/100 catheter days) and complete occlusions (0.09/100 catheter days). The system was thought to be safe and convenient in chemotherapy, and permitted full physical activity.
Anti-Bacterial Agents ; Antineoplastic Agents ; Catheters ; Child* ; Drug Therapy ; Humans ; Motor Activity ; Needles ; Prospective Studies*