Nitric oxide (NO) induces apoptotic cell death in murine RAW 264.7 macrophages. To elucidate the roles of SEK1/MKK4, a upstream kinase for both c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) and p38 kinase, on NO-induced apoptosis, we generated clones of RAW 264.7 cells which stably overexpressd kinase inactive SEK1 (RAW/SEK1-Kl) or wild type SEK1 (RAW/SEK1-WT). Treatment of kinase inactive SEK1 transfected RAW 264.7 cells (RAW/SEK1-Kl) with sodium nitroprusside (SNP), a NO generating agent, significantly decreased the cell viability up to 20% of RAW control cells which were treated with the same amount of SNP. However, RAW/SEK1-WT cells were less susceptible to NO induced apoptosis. For a while, caspase-3 like activity in NO treated RAW/SEK1-Kl cells was significantly increased with parallell to apoptotic death rate. However, caspase1 like activity was not affected by NO in any transfectants. The NO induced apoptosis in RAW/SEK1-Kl cells was significantly prevented by the addition of caspase-3 like inhibitor (N-Ac- DEVD-CHO). In addition, the phosphotransferase activity of JNK1 in NO-treated RAW/SEK1-WT is significantly increased, but not in RAW/SEK1-Kl cells. These results suggest that SEK1 may play anti-apoptotic role in RAW cells from NO-induced apoptosis.
Apoptosis ; Caspase 3 ; Cell Death ; Cell Survival ; Clone Cells ; Macrophages ; Mortality ; Nitric Oxide* ; Nitroprusside ; Phosphotransferases ; Protein Kinases

The effect of topical steroid application on clinical results of 215 eyes(126 patients) performed with excimer laser photorefractive keratectomy (PRK) from July 1993 to July 1994 was evaluated, postopertively. The range of myopia was between -2.5 and -11.5 diopter and followed for 9 months after PRK with Summit Omnimed(TM). Topical corticosteroid or non-steroidal antiinflammatory drug (NSAID) was not applied for one month after operation in all cases. After one month, in cases of 115 eyes showing myopic regression of more than -1 diopter or stromal haziness of more than grade 1(delayed steroid group; DSG), 0.1% fluorometholone was applied 4 times daily for 4 weeks, then tapered according to the planned schedule. 46.5% (100 eyes, non-steroid group;NSG) did not show the myopic regression nor stromal haziness without treatment of fluorometholone throughout the follow-up period. Uncorrected visual acuity, spherical equivalent and corneal haziness were evaluated 1, 2, 4, 8, 12, 24, 36 week after operation. 73%(73 eyes) of NSG and 27%(30 eyes) of DSG showed a myopia less than 6.0 diopters preoperatively. The proportion of male was 36% in NSG and 50.4% in DSG. 88.1% of male high myopia(>6.0 diopters) needed 0.1% fluorometholone, while 17.6% of female moderate and mild myopia( Appointments and Schedules ; Female ; Fluorometholone ; Follow-Up Studies ; Humans ; Incidence ; Lasers, Excimer* ; Male ; Myopia ; Photorefractive Keratectomy* ; Visual Acuity

Aplasia cutis congenita is a rare disorder characterized by localized or generalized absence of skin at birth. The various hypothesis and classification are proposed. Type V in Frieden's classification, which is associated with fetus papyraceus or placental infarcts, occurs as a large cutaneous defect on the trunk and extremities. We experienced the patient had skin defect affecting the lower abdomen symmetrically, with no family history of the any disorders or chromosomal anomalies and associated anomalies. A twin fetus was died in utero. We report a case of aplasia cutis congenita associated with fetus papyraceus with the review of the associated literatures.
Abdomen ; Classification ; Ectodermal Dysplasia* ; Extremities ; Fetus ; Humans ; Parturition ; Skin ; Twins

No abstract available.
Eosinophil Cationic Protein* ; Eosinophils* ; Rhinitis*

No abstract available.
Osteogenesis Imperfecta* ; Osteogenesis*

OBJECTIVES: A case-control study was conducted on 2,323 male soldiers(771 herniated nucleus pulposus patients and 1542 controls) to identify risk factors for cumulative trauma disorders especially lower back. METHODS: The collecting data included individual physical, psychological, and work environmental factors. Korea Military Personality Inventory(KMPI) was used to examine the assess trait that have been associated with clinically with herniated nucleus pulposus. A measurement of the workplace support system was obtained by using of the modified work APGAR, a brief, seven-item workplace function questionnaire. RESULTS: The herniated nucleus pulposus patients were more common among Artillerymen and Engineer than among infantrymen. In logistic regression analysis of military occupational specialties, Artillerymen, Engineer, radio operator, driver were higher risk of herniated nucleus pulposus than infantrymen. Multivariate analysis of KMPI scales found hypochondriasis scale, hysteria scale, hypomania scale, social introversion scale to have strong relationship to incidence of herniated nucleus pulposus. And 'can communicate with peers' and 'enjoy job task' item of modified work APGAR to have strong relationship to incidence of herniate nucleus pulposus CONCLUSIONS: Study of the relative contributions of many physical and non physical variable shows that evaluations of back problems in workplace that exclude these highly significant work perception and psychosocial variables are of limited value. Simple, unidimensional approaches that ignore the effect of work perceptions and psychological factors on back problems reporting oversimplify a multifaceted problem. These findings emphasize the importance of adopting a broader approach to the multifaceted problem of back complaint in workplace.
Case-Control Studies ; Cumulative Trauma Disorders ; Humans ; Hypochondriasis ; Hysteria ; Incidence* ; Introversion (Psychology) ; Korea ; Logistic Models ; Male ; Military Personnel ; Multivariate Analysis ; Psychology ; Questionnaires ; Risk Factors ; Weights and Measures

No abstract available.
Female ; Pregnancy ; Pregnancy, Ectopic*

No abstract available.
Colitis, Ulcerative* ; Ulcer*

The authors studied on anxiety, using Zung's self-rating anxiety scale (SAS), on the subjects of 329 men and 522 women of 60 years old and older. This study was carried out for 8 months from November 1983 to June 1984 in the area of Seoul, Taegu and Kyungsan Gun, Kungpook province. The results showed that there was no significant difference in the means of total anxiety scores between the two groups of men and women. The anxiety scores in items of sweating, apprehension, dyspnea, restlessness and insomnia were relatively high in both groups. The scores of faintness, panic, fear, tremor and facial flushing were low in both groups. Among 329 men, 48 (14.6%) showed rather serious anxiety level of score 50 or higher, while 153 of 522 women (29.4%) showed the same score. Among these psychosocial factors, supporter, living, place, religion and security system are significantly related with anxiety scores in both groups (p<0.01).
Anxiety* ; Daegu ; Dyspnea ; Female ; Flushing ; Gyeongsangbuk-do ; Humans ; Male ; Mental Health* ; Panic ; Psychology ; Psychomotor Agitation ; Seoul ; Sleep Initiation and Maintenance Disorders ; Sweat ; Sweating ; Tremor

No abstract available.
Doxorubicin* ; Drug Therapy* ; Small Cell Lung Carcinoma*