1.Two Case of Systemic Candidiasis in Premature Infants.
Dae Kyun KIM ; Woo Chul SUH ; Eun Gyeoung JUNG ; Eun Seok YANG ; Sang Kee PARK
Journal of the Korean Pediatric Society 1995;38(11):1558-1564
No abstract available.
Candidiasis*
;
Humans
;
Infant, Newborn
;
Infant, Premature*
2.Sequence Generation and Genotyping of 15 Autosomal STR Markers Using Next Generation Sequencing.
Eun Hye KIM ; Sang Eun JUNG ; Kyoung Jin SHIN ; Woo Ick YANG ; In Seok YANG
Korean Journal of Legal Medicine 2014;38(2):48-58
Recently, next generation sequencing (NGS) has received attention as the ultimate genotyping method to overcome the limitations of capillary electrophoresis (CE)-based short tandem repeat (STR) analysis, such as the limited number of STR loci that can be measured simultaneously using fluorescent-labeled primers and the maximum size of STR amplicons. In this study, we analyzed 15 autosomal STR markers via the NGS method and evaluated their effectiveness in STR analysis. Using male and female standard DNA as single-sources and their 1:1 mixture, we sequentially generated sample amplicons by the multiplex polymerase chain reaction (PCR) method, constructed DNA libraries by ligation of adapters with a multiplex identifier (MID), and sequenced DNA using the Roche GS Junior Platform. Sequencing data for each sample were analyzed via alignment with pre-built reference sequences. Most STR alleles could be determined by applying a coverage threshold of 20% for the two single-sources and 10% for the 1:1 mixture. The structure of the STR in each allele was accurately determined by examining the sequences of the target STR region. The mixture ratio of the mixed sample was estimated by analyzing the coverage ratios between assigned alleles at each locus and the reference/variant ratios from the observed sequence variations. In conclusion, the experimental method used in this study allowed the successful generation of NGS data. In addition, the NGS data analysis protocol enables accurate STR allele call and repeat structure determination at each locus. Therefore, this approach using the NGS system will be helpful to interpret and analysis the STR profiles from singe-source and even mixed samples in forensic investigation.
Alleles
;
DNA
;
Electrophoresis, Capillary
;
Female
;
Gene Library
;
Humans
;
Ligation
;
Male
;
Microsatellite Repeats
;
Multiplex Polymerase Chain Reaction
;
Statistics as Topic
3.A Case Report of a Child who has Attention Deficit Hyperactivity Disorder, Mental Retardation, and Mania.
Korean Journal of Psychopharmacology 2003;14(4):397-404
It has been known that childhood bipolar disorder is rare, especially combined with attention deficit hyperactivity disorder (ADHD). But, recent studies reported that childhood bipolar is more common than our guess. Many symptoms of childhood mania overlapped with ADHD, so it is difficult to differentiate from ADHD. It also often reported that psychostimulants, medication for ADHD, induce manic symptoms. Clinically, childhood mania has several characteristics. It is frequently chronic, mixed type, rapid cycling and often refractory to treatment. So it is important to diagnose, differentiate, treat childhood bipolar disorder and ADHD, but there is no report about comorbidity, treatment, clinical characteristics about that. We report a patient combined ADHD, mental retardation and childhood bipolar disorder.
Attention Deficit Disorder with Hyperactivity*
;
Bipolar Disorder*
;
Child*
;
Comorbidity
;
Humans
;
Intellectual Disability*
4.Effects of Aging and Smoking Duration on Cigarette Smoke-Induced COPD Severity
Eun Jung KIM ; Seok Jun YOON ; Young Eun KIM ; Dun Sol GO ; Yunsun JUNG
Journal of Korean Medical Science 2019;34(Suppl 1):e90-
BACKGROUND:
Aging can serve as an underlying mechanism of chronic obstructive pulmonary disease (COPD). Also, smoking, which is the most common cause of COPD, is responsible for the systemic manifestations of the disease, independently from the lung function alterations. The purpose of this study was to analyze the effect of aging on the occurrence of cigarette smoking induced COPD.
METHODS:
For this analysis, we evaluated smoking status by a lifestyle intervention program and measured the occurrence of COPD by the Korea National Health and Nutrition Examination Survey (KNHANES) from 2005 to 2015.
RESULTS:
Aging and smoking were significantly effected on the lung function of COPD patients. Especially, the smoking duration is exaggerated in the presence of old age for older COPD patients.
CONCLUSION
The result showed that COPD patients exhibit aging and smoking duration related severity. The prevalence of COPD kept increasing internationally. Knowing the risk factor of COPD quantitatively and finding out the interaction among risk factors could be valuable predictors for preventing COPD.
5.Corrigendum: Moderate and Deep Hypothermia Produces Hyporesposiveness to Phenylephrine in Isolated Rat Aorta.
Jun Woo CHO ; Chul Ho LEE ; Jae Seok JANG ; Oh Choon KWON ; Woon Seok ROH ; Jung Eun KIM
The Korean Journal of Thoracic and Cardiovascular Surgery 2014;47(1):75-75
There was an error in article.
6.In vitro Stimulation of Tumor - Draining Lymph Node Lymphocytes with the 30 kDa Antigen of Mycobacterium tuberculosis Leads to the Differentiation of Th1 Cells and Cytotoxic Effector Cells.
Jeong Kyu PARK ; Tae Hyun PAIK ; Seok Shin KOH ; Hwa Jung KIM ; Eun Kyeong JO
Korean Journal of Immunology 1997;19(1):59-72
Tumor-draining lymph node (TDLN) lymphocytes contain immunologically sensitized to tumor but functionally deficient T cells. The 30 kDa protein antigen, a major secreted protein antigen of Mycobacterium tuberculosis, exhibits strong T cell stimulatory effect. In this study, it examined that the feasibility of using M tuberculosis 30 kDa antigen to stimulate tumor-draining lymph node cells for the generation of specific immune effector cells. Freshly isolated TDLN lymphocytes could directly respond to the 30 kDa antigen alone and their proliferative responses were markedly augmented by stimulation with rIL-2. TDLN cells were stimulated with the 30 kDa antigen for various time intervals and examined for the induction of IFN-r and IL-4 mRNA using RT-PCR. The expression of IFN-r mRNA was greatly augmented after 1 wk, whereas IL-4 mRNA is markedly decreased after 1 wk. Cytotoxic T cell activities induced by the 30 kDa antigen was also evaluated. TDLN cells stimulated with the 30 kDa antigen alone were able to generate remarkable cytotoxic response to K562 or Daudi cell lines after 6 days of culture. And their cytotoxic effects were highly augmented by stirnulation with rIL-2. These results suggest that the 30 kDa antigen of M. tuberculosis may selectively activate Thl cells of TDLN lymhocytes and induce the cytotoxic T cell activities. In conclusion, the 30 kDa antigen can be used as a biologic response modifier in tumor immunology.
Allergy and Immunology
;
Cell Line
;
Interleukin-4
;
Lymph Nodes*
;
Lymphocytes*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
;
RNA, Messenger
;
T-Lymphocytes
;
Th1 Cells*
;
Tuberculosis
7.Effect of Body Position on Gastric Emptying.
Joong Man KIM ; So Ra LEE ; Jung Eun SEOK ; Yeon Kyun OH
Korean Journal of Perinatology 2000;11(1):33-38
No abstract available.
Gastric Emptying*
8.Ossifying Fibromyxoid Tumor of Soft Parts.
Seok Hoon JEON ; Seung Sam PAIK ; Eun Kyung HONG ; Moon Hyang PARK ; Jung Dal LEE
Korean Journal of Pathology 1997;31(2):174-178
An ossifying fibromyxoid tumor of soft parts is a rare, recently described, fibro-osseous neoplasm of uncertain histogenesis. It occurs most frequently within the subcutis or skeletal muscle of the extremities. Its biologic behavior is generally regarded as benign with at worst a locally aggressive clinical course. But, atypical and malignant variants have been recently reported. Herein we report a case of a benign ossifying fibromyxoid tumor which occurred in the left upper back of 41-year-old man. The tumor is composed of uniformly round or polygonal cells arranged in cords or nests which are separated by myxoid and hyalinzed fibrous matrix and associated with irregular bony trabeculae. The tumor cells are strong positive for vimentin. Ultrastructural findings and a review of literatures are added.
Adult
;
Extremities
;
Humans
;
Muscle, Skeletal
;
Vimentin
9.Ossifying Fibromyxoid Tumor of Soft Parts.
Seok Hoon JEON ; Seung Sam PAIK ; Eun Kyung HONG ; Moon Hyang PARK ; Jung Dal LEE
Korean Journal of Pathology 1997;31(2):174-178
An ossifying fibromyxoid tumor of soft parts is a rare, recently described, fibro-osseous neoplasm of uncertain histogenesis. It occurs most frequently within the subcutis or skeletal muscle of the extremities. Its biologic behavior is generally regarded as benign with at worst a locally aggressive clinical course. But, atypical and malignant variants have been recently reported. Herein we report a case of a benign ossifying fibromyxoid tumor which occurred in the left upper back of 41-year-old man. The tumor is composed of uniformly round or polygonal cells arranged in cords or nests which are separated by myxoid and hyalinzed fibrous matrix and associated with irregular bony trabeculae. The tumor cells are strong positive for vimentin. Ultrastructural findings and a review of literatures are added.
Adult
;
Extremities
;
Humans
;
Muscle, Skeletal
;
Vimentin
10.A comparative trial of Nalador and mechanical stimulation(Metreurynter) in the termination of midtrimester pregnancy.
Jung Ja JIN ; Eun Ju CHANG ; Jae Seok LEE ; Keon JIN ; Dong Jin KIM
Korean Journal of Obstetrics and Gynecology 1992;35(5):682-693
No abstract available.
Female
;
Humans
;
Pregnancy
;
Pregnancy Trimester, Second*
;
Pregnancy*