BACKGROUND: Desflurane is a new inhaled anesthetic with the lowest blood/gas partition coefficient and enflurane is one of the major anesthetics in these days. But the effect of volatile anesthetics and the site of action on the blood vessel are still controversial. Since Furchgott (1980) discovered endothelium derived relaxing factor (EDRF) from endothelium, many investigators have studied about the relationship between the EDRF and the effect of the volatile anesthetics on blood vessels. In this study, we evaluated that the effect and the action site of enflurane and desflurane on isolated aortic rings of the rabbit. METHODS: Each of obtained thoracic aorta from rabbits (1.5~2.5 kg) was divided into 4~6 mm rings, and a half of that were denuded. All of the aortic rings were preconstricted with phenylephrine 1.5 10-7 Mole in warm organ bath filled with modified Krebs' solution, and then LNAME (inhibitor of nitric oxide synthase, 3 10-4Mole) was administered to one group of aortic rings. MB (inhibitor of soluble guanylyl cyclase, 2 10-5Mole) was administered to another one group and neither of LNAME nor MB was administered to the other group. And then enflurane (1%, 2%, 3%, 4%) or desflurane (6%, 9%, 12%) was administered to all of aortic rings. The polygraph recorded the changes of tension of aortic ring which was transmitted through the force transducer. RESULTS: It was proved that basal EDRF was released from endothelium by the fact that intact aortic rings were more constricted after LNAME or MB administration. The intact aortic rings were constricted in all concentration of enflurane and both intact and denuded rings were maintained from control tension in all concentrations of desflurane. CONCLUSION: It is concluded that enflurane in all concentrations has an endothelium-mediated vasoconstriction effect and desflurane in all concentrations has no effect on isolated aortic rings of rabbit.
Anesthetics ; Aorta, Thoracic ; Arginine* ; Baths ; Blood Vessels ; Endothelium ; Endothelium-Dependent Relaxing Factors ; Enflurane* ; Guanylate Cyclase ; Humans ; Nitric Oxide Synthase ; Phenylephrine ; Rabbits ; Research Personnel ; Transducers ; Vasoconstriction

No abstract available.
HLA-DR Antigens* ; Polymerase Chain Reaction* ; Spermatozoa*

To know the amplification pattern according to relative concentration ratio in mixed samples, two STRloci, vwF locus and MBP locus and two VNTR loci, D1S80 locus and d17S5 locus were amplified in DNA with various concentration of two individuals were easily identified. But when the concentration of one person were lowered to 1/20-1/40 of the other's the intensity of product bands diminshed and hardly discernible. Also different amplification efficiency according to the template length was noted, especially in VNTR loci. Using automatic sequencer and RFLP scan program, the intensity OD of each PCR product band could be calculated, and this correlates the felative amplification efficiency of each allele. By using this we could construct quantitative PCR for the mixed samples. This could be used in practical case work for forensic purpose, and also be a valuable candidate for 'chimerism detection' in case of bone marrow transplatation.
Alleles ; Bone Marrow ; DNA ; Humans ; Minisatellite Repeats ; Polymerase Chain Reaction* ; Polymorphism, Restriction Fragment Length

No abstract available.
HLA-DR Antigens* ; Polymerase Chain Reaction*

The keratinized mucosa around the implant is an important key in health of soft tissue and hard tissue. The purpose of this study is showed that the keratinized mucosa is associated with the keratinized mucosa index, plaque index, gingival index, probing depth. which is investigated to observing the peri-implant mucosa of mandibular partial edentulous patuent using periodontal parameter by previously published paper. It was estimated 6 site with regard to 80 fixture for 28 person, and the average age is 46.8. Each estimation is the order of less trauma, that is, plaque index, keratinized mucosa index, gingival index and probing depth. In this study, statstically analyzed treatment is used for Spss V 7.0 for Windows(Spss Inc, USA). The Kruskal Walis Test is used to compare the amount of the keratinized mucosa is into the 0~3 index, with plaque index, gingival index and probing depth. Mann-whitney Test is used to interpreate the relation of plaque index and probing depth, which is showed significant difference. The Result are as follows 1. The kertinized mucosa index 3 amounts to 47.7%, which is much higher than the other indices and the index order is followed 3, 1, 2 and 0. 2. The plaque index 1 amounts to 61.7%, which is much higher than the other indices and the index order is followed 1, 2, 3 and 0. The plaque index 0 is significant to each of index(P<0.05). The plaque index is decrease as the keratinized mucosa index is increased. 3. The probing depth for 2mm, 1mm, 3mm is 48.9%, 23.5%, 16.8% respectively, which is most occupied. The probing depth 2mm and 3mm for the keratinized mucosa index is significant(P<0.05). The probing index is decreased as the keratinized mucosa index is increased. 4. The gingival index 0 amounts to 58.0%, which is much higher than the other indices and the index order is followed 0, 1, 2 and 3.
Humans ; Mucous Membrane ; Periodontal Index

The purpose of this study was to evaluate clinical changes in graft size after treatment with strip gingival autograft in human. 57 premolar teeth in 27 patients having the following mucogingival problems were selected. The width of extension, attached gingiva including free marginal gingiva, width of transplant and clinical sulcus depth were measured at the initial examination, 2, 12 and 24 weeks following the strip gingival autograft and free gingival autograft. The change of width of extension, attached gingiva including free marginal gingiva, width of transplant and clinical sulcus depth according to healing process in both graft procedures was statistically analyzed by repeated measure ANOVA test and independent t-test using SPSS program. The results were as follows : 1. The change of keratinized gingiva in both graft procedures was increased significantly at 24 weeks post-op. 2. The clinical sulcus depth exhibited no marked changes throughout the entire investigation in both graft procedures. 3. No dimensional variation was seen in graft size in both graft procedures. 4. Shrinkage did not differ significantly in both graft procedures. From the day of grafting to 24 weeks after surgery the percentages of shrinkage were : strip gingival autograft 28% and free gingival autograft 29%.
Autografts* ; Bicuspid ; Gingiva ; Humans ; Tooth ; Transplants*