BACKGROUND: Lumbar spinal nerve root compression causes the damage of neural function and abnormal neural action of lumbar spinal nerve root, which is related not only to mechanical injury of the compressed but also to inflammatory reaction.OBJECTIVE: To establish the animal model of lumbar nerve root compression and investigate the effect of acupuncture on the ultramicrostructure of the injured lumbar nerve and inflammatory medium of rats.DESIGN: A randomized controlled trial.SETTING: Department of Acupuncture & Moxibustion, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University.MATERIALS: The study was conducted in the Experimental Animal Center of Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University from January 2001 to January 2004. Totally 90 healthy male Wistar rats were randomly divided into model group and model + electroacupuncture group with 45 rats in each.METHODS: Clominorex was injected intraperitoneally for anesthesia.With interspace of spinous process of L5-6 as the center, the special silica gel slice was placed where the right side of L5 nerve root ran out of the dura mater sac, stabilized, sutured layer by layer, and covered with the aseptic gauze. Modeling group: No special disposal was made and the rats were fed regularly after modeling. Modeling + electro-acupuncture group:One day after modeling, a filiform needle of 28 gauge and 3.3 cm (1 inch)was used to puncture Huantiao and Yanglingquan points of the right hindlimb and Jiaji point of the right side of the oppressed nerve root. The oppressed injured lumbar nerve tissue was disposed after 15 days of modeling and 30 daysof modeling + electro-acupuncture, and observed with the optic microscope and electron microscope. The content of inflammatory medium such as prostaglandin E2 and5-hydroxy tryptamine was measured.MAIN OUTCOME MEASURES: [1] Results of damaged lumbar nerve root tissues under the electron microscope and light microscope; [2] content of inflammatory medium such as prostaglandin E2 and 5-hydroxy tryptamine.RESULTS: All the 90 rats entered the final analysis. [1] Hyperemia around the nervous tissues, edema and nerve root demyelination of electroacupuncture 15 days (model + electro-acupuncture group) were relieved.The structure of Schwann cells was normal. Massive inflammatory cells gathered around the nervous tissues in 30-day model group (model group).There was severe demyelination of nerve root tissues, and the gap between the nervous tissues widened and red blood cells leaked out. Demyelination of electro-acupuncture 30-day group (model + electro-acupuncture group)became mild and the edema in nerves was alleviated. [2] The content of prostaglandin E2 in the locally oppressed rat lumbar nerve tissues of 15-day and 30-day model + electro-acupuncture groups was obviously lower than that in 15-day and 30-day model groups [(61.17±9.77), (76.65±7.39) ng/L;(56.05±7.98), (71.04±6.99) ng/L, P ＜ 0.01], while the content of 5-hydroxy tryptamine in the locally oppressed rat lumbar nerve tissues of 15-day and 30-day model + electro-acupuncture groups was obviously lower than that in 15-day and 30-day model groups [(1 413.4±194.5), (2 587.7±214.7) ng/L;(780.4±133.0), (1 355.0±147.7) ng/L, P ＜ 0.01].CONCLUSION: Electro-acupuncture can alleviate inflammatory reaction around the injured lumbar nerves and improve the ultramicrostructure of lumbar nerve root. It has the effect of decreasing the content of prostaglandin E2 and 5-hydroxy tryptamine in oppressed lumbar nerve root tissues and partially inhibiting inflammatory reaction.

The monitoring of total toxicity of water is of great importance to guarantee the safety of drinking water and estimate the level of water pollution from multiple pollutants.Here, we reported an online equipment for monitoring toxicity of water, where in-site cultured microorganisms were used as the test organism and respiratory inhibition of microorganisms was regarded as the toxicity response.A three-electrode electrochemical detector was integrated in the equipment, where an array electrode composed of microsized Pt disks, a Ag/AgCl (3 mol/L KCl) electrode and a gold wire were used as the working, reference and counter electrodes, respectively.The average respiratory inhibition ratios obtained with this equipment for 5.0, 10.0, 20.0 mg/L 3,5-dichlorophenol (DCP) were 24.4%, 48.9% and 59.2%, respectively.The equipment was further used for continuous online monitoring of the total toxicity of underground water, and successfully showed the toxicity alarm when challenged randomly by toxicity samples containing 5.0 mg/L DCP.The present results suggested the good promise of the equipment in online monitoring of water total toxicity.

OBJECTIVE:To establish a method for simultaneous determination of pulchinenoside B4,caffeic acid,baicalin, palmatine hydrochloride and berberine hydrochloride in Baipuhuang tablets. METHODS:HPLC method was adopted. The determi-nation of Agela Technologies Venusil XBP C18(L)with mobile phase consisted of acetonitrile-0.05 mol/L monopotassium phosphate (gradient elution)at the flow rate of 0.8 mL/min. The detection wavelengths were set at 203 nm(pulchinenoside B4)and 323 nm (caffeic acid,baicalin,palmatine hydrochloride,berberine hydrochloride). The column temperature was set at 30 ℃,and the sam-ple size was 10 μL. RESULTS:The linear ranges were 0.08141-8.141μg for pulchinenoside B4(r=0.9998),0.01871-1.871 μg for caffeic acid (r=0.9994),0.03733-3.733 μg for baicalin (r=0.9992),0.02885-2.885 μg for palmatine hydrochloride (r=0.9996) and 0.02758-2.758 μg for berberine hydrochloride (r=0.9997). The limits of quantitation were 0.009,0.006,0.008, 0.011,0.013 ng,respectively. The limits of detection were 0.030,0.020,0.025,0.034,0.036 ng,respectively. RSDs of preci-sion,stability and reproducibility tests were all lower than 2.0%. The average recoveries were 97.39%-102.34%(RSD=1.81%,n=6),96.77%-98.92%(RSD=0.85%,n=6),97.38%-103.72%(RSD=2.46%,n=6),96.73%-102.01%(RSD=2.22%,n=6)and 96.47%-101.07%(RSD=1.61%,n=6),respectively. CONCLUSIONS:The method is simple,accurate and reproducibility,and can be used for simultaneous determination of pulchinenoside B4,caffeic acid,baicalin,palmatine hydrochloride and berberine hy-drochloride in Baipuhuang tablet.

Objective To explore the clinical characteristics of rosacea in Xinjiang.Methods From Jan 2013 to Jun 2015,clinical data of hospitalized patients who were diagnosed in the People's Hospital of Xinjiang Autonomous Region as rosacea were analyzed retrospectively.Results (1) The onset age of Uygur and Han were similar,while the incidence of Han was higher than Uygur in 41 ～ 50 age group.(2) The ratio of outdoors was higher in Uygur patients than Han patients.(3) Reactive erythema was the mainly feature in Han patients with obviously self-conscious symptoms including burning,drying,and tingling.The Uyghur patients were present with persistent erythema,papules and pustules;and had facial swelling and mild symptoms.(4) Positive rates of thyroid related antibodies and prolactin were higher in patients with rosacea.Conclusions The clinical features of rosacea are different between Uygur and Han nationalities in Xinjiang.

Objective To study the effects of community nursing interventions on stroke patients with attention disorders.Methods A total of 60 stroke patients fulfilling eligibility were divided randomly into two groups.Their data were collected between December 2010 and February 2012.The intervention group was treated by community nurses while the control group trained by family members.Attention was assessed by simple reaction time (SRT) and number cancellation test (NCT).Modified Barthel index was used to assess the activities of daily living (ADL).Both groups were evaluated before and after a 2-month intervention.Results Before treatment,the control group had(0.71 ± 0.25),(662.8 ± 152.7) and(31 ±8)scores at SRT,NCT and MBI respectively.And the intervention group yielded(0.69 ± 0.19),(652.7 ±131.0) and (30 ± 8)scores at SRT,NCT and MBI respectively.There were no significant differences in average value of SRT,NCT and MBI between training and control groups at pre-treatment (P ＞ 0.05).After 2 months,the control group had (0.68 ± 0.20),(637.4 ± 151.7) and (33 ± 8) scores at SRT,NCT and MBI respectively.And the intervention group yielded (0.56 ± 0.16),(540.3 ± 125.9) and(37 ± 8) scores at SRT,NCT and MBI respectively.Very significant difference existed in attention scale scores at Month 2 (P ＜ 0.01) and the difference in ADL scale scores was also significant (P ＜ 0.05).Conclusion For stroke patients with attention disorders,strengthening community nursing interventions may help to improve attention and boost activity of daily living.

Objective To explore the role of enteral nutrition in the treatment of functional delayed gastric emptying. Methods Among 15 patients with functional delayed gastric emptying after resection of esophageal or gastric cardiac carcinoma, 10 patients were treated with enteral nutrition( EN group),5 patients were treated with parenteral nutrition (PN group). Postoperative hospital stay, gastrointestinal decompression amount, recovering time of postoperative gastric emptying were observed to assess the efficacy of enteral nutrition. Results The average postoperative hospital stay was ( 14. 4 ± 4. 6) days in the EN group, whereas (20. 3 ±6. 6) days in the PN group. The average recovering time of postoperative gastric emptying was (19 ±9)days in the PN group and( 12 ± 4)days in the EN group. Conclusion The method of EN can enhance gastric emptying and is effective for functional delayed gastric emptying after resection of esophageal or gastric cardiac carcinoma

Objective:To evaluate the prediction value of preoperative Fibrinogen(FIB) in combination with neutrophil-lymphocyte ratio(NLR)for the prognosis of operable pancreatic cancer patients.Methods:The clinical data of 124 patients who underwent radical resection for pancreatic cancer in our hospital from Jan 2010 to Dec 2018 were retrospectively analyzed.The patients were divided into three groups according the high and low NLR, FIB value which defined by the receiver operating characteristic curve (ROC). The clinicopathological data and overall median survival time were compared between the three groups.Results:Univarate analysis showed that age, tumor stage, NLR, FIB and F-NLR score were associated with the prognosis( P<0.05), while multivariate analysis showed that high F-NLR score was the independent prognostic factor. The median survival time of patients with F-NLR scores 0, 1 and 2 group was 30.6, 20.3 and 13.9 months( P<0.05). The prognosis of high F-NLR score was significantly worse than that of low F-NLR score( P<0.05). Conclusions:A high preoperative F-NLR score was a promising predictor for the prognosis of pancreatic cancer patients after radical resection.

Objective:To evaluate effects of different medication forms of dexmedetomidine on perioperative cognitive function in elderly patients undergoing radical operation of gastric carcinoma, and Ramsay sedation score.Methods:A total of 150 patients aged 65 years and 75 years scheduled for elective radical operation of gastric carcinoma from December 2018 to December 2019 in Shanxi Provincial Cancer Hospital were enrolled, and they were divided into 3 groups according to random number table method: different medication forms of dexmedetomidine groups (group A, group B) and the control group (group C), 50 cases in each group. The patients in group A and group B continued to pump dexmedetomidine at a rate of 0.5 μg·kg -1·h -1 and 0.3 μg·kg -1·h -1 respectively for 15 min before induction of anesthesia. And the patients in group C were given intravenous pumping of an equal volume of 0.9% NaCl for 15 min. Subsequently, patients in group A and group B received continuous intravenous infusion of 0.3 μg·kg -1·h -1 for 30 min before the end of operation, the patients in group C received 0.9% NaCl infusion of equal volume until the end of operation. The cognitive function of the patients was measured by using the Monterey cognitive assessment scale (MoCA) the day before surgery and on day 1,3 and 7 after surgery, and the incidence of perioperative neurocognitive disorder (PND) was counted. Ramsay sedation score at 30 min, 24 h and 48 h after surgery was compared among the three groups. Results:There were 3 patients in group A and 3 patients in group C were excluded because they were transferred to ICU due to serious postoperative complications. There were significant differences in MoCA score among the three groups on day 1, 3, 7 after operation (all P < 0.01); MOCA score of group A, B and C on day 7 after operation was (26.9±0.7) scores, (26.6±1.0) scores, (26.3±1.2) scores, respectively, and the difference between group A and group C was statistically significant ( P < 0.01). The incidence of PND among the three groups on day 1, 3, 7 after surgery had statistically significant differences (all P < 0.05), and the incidence of PND in group A was lower than that in group B and C (all P < 0.05). Ramsay sedation score among the three groups at 30 min and 24 h, 48 h after operation showed statistically significant differences (all P < 0.01), and that in group C was lower than that in group A and group B, and the differences were statistically significant (all P < 0.01). Ramsay sedation score at 24 h after operation in group A was high than that in group B and group C (all P < 0.01). Conclusions:Dexmedetomidine assisted with anesthesia can reduce the incidence of PND in elderly patients undergoing radical operation of gastric carcinoma and enhance the sedative effect. What's more, the most obvious effect is the infusion of 0.5 μg·kg -1·h -1 before anesthesia induction.

AIM: To compare the renoprotective effects of angiotensinⅡtypeⅠreceptor antagonist (AT 1RA) with that of angiotensin converting enzyme inhibitor (ACEI) and to investigate their influences on intrarenal renin-angiotensin system. METHODS: Experimental nephrotic syndrome model was induced in SD rats with repeated peritoneal injections of puromycin. Twenty-eight rats were randomly divided into four groups: normal control, nephrotic control, ACEI-treated and AT 1RA-treated group. Serum, urine and renal tissue were collected for study 12 weeks later. RESULTS: The urine protein was less and renal function was better in both treated groups. The glomerular and interstitial injury indexes of both ACEI and AT 1RA treated rats were lower than that of nephrotic control rats and had no significant difference between the two treated groups. The renal local ACE activity and angiotensinⅡ of nephrotic control group were significantly higher than that of normal control group and the two treated group(P

Recepteur d′origine Nantais(RON),a tyrosine kinase receptor ,is a growth factor receptor belonging to the proto-oncogene met family and has been proved to display abnormal expres?sion in many types of tumors. The RON receptor is activated by binding to the ligand macrophage stim?ulating protein,overexpression of the receptor,variants of the proto-oncogene and by point mutations of the kinase region. The downstream transduction of RON by mitogen-activated protein kinases and phosphoinositide3-kinase signaling pathways can help regulate the proliferation,apoptosis,migration and invasion of tumor cells. A better understanding of the mechanisms and related signaling pathways of RON activation in tumor progress and development will provide more information for the RON-based target therapy.