Objective;Discussing clinical utilization of Automation Urine Analyszer in the testing Hydrothorax and Hydroperitonium. Methods:Testing 80 specimen got from our clinci and patient by using Uritest-200 equipemnt to get the results of PRO and SC, using UF - 100 to get the results of RBC,WBC,EC which can be compared with the results which got by handwork. Results:Comparison between Uritest-200 and handwork method, PRO and SG usex2 examination, the results have very siginficant differ( P

Objective:To study the resistance and its tendency of E.coli to antibiotics was to serve the rational administration in clinic.Methods:WHONET5 was employed to analyze the drug sensitivities of 616 E.coli isolated from the clinical specimens from January,2004 to July,2006 in hospital.Results:The resistance rates of E.coli to Ampicillin,Piperacillin,Amoxicillin/cla,Trimethoprim/Sulfamethoxazole and Ciprofloxacin exceeded 60%,and they were about 50% to Gentamicin,Cefazolin and Cefuroxime.In addition,the resistant rates were20% infra to Piperacillin/tazobactam,Cefepime,Ceftazidime,Cefoxitin,Amikacin and Furantoin,and there was no resistance to Imipenem.The detection rates of ESBLs to E.coli was 27.96%,32.68%,35.12% in 2004,2005,2006 respectively.Conclusions:The resistance rates of E.coli to Penicillins,Piperacillins/Clavulanic acid,Sulfanilamide,Quinolone,Gentamicin,the first and second generations Cephalosporins were high,and they were not suitable for clinical use.To Piperacillins/tazobactam,fourth generation Cephalosporins,Cephamycins,Imipenem,Amikacin and Furantoin,the resistance rates were relatively low,and they could be used clinically.The third generation Cephalosporins should be used cautiously due to its feasibility of inducing ESBLs even though its resistant rates were low.The production rate of ESBLs of E.coli ascended year by year.

0.05.Conclusion Using XT-2000i Automated Hematology Analyzer to test the reticulocyte the reproducibility is better,and the linearity can content well with clinic,the rate of carry contamination is low,the stability is better too.Compared XT-2000i with the manual methods,the relativity is better. It can replace the manual methods.

Objective Analyzed the cause and time of hypokalemia complication that dexamethasone used for hyperthyroidism preoperative preparation.Methods The study consisted of 161 patients with hyperthyroidism disease,who were used the dexamethasone in his or her preoperative preparation at the First Affiliated Hospital of Zhengzhou University between August 2011 and July 2013.Results In the 161 hyperthyroidism patients,composed of 41 men and 120 women,twenty-seven patients happened hypokalemia and 92.6％ occurred after the first.Twenty-six men patients developed hypokalemia complication when used dexamethasone for hyperthyroidism preoperative preparation,while only 1 woman patient occurred the hypokalemia complication.Among the 11 patients who with a history of thyrotoxic periodic paralysis,10 cases occurred after given dexamethasone hypokalemia,only 1 case has no hypokalemia.Conclusion Dexamethasone and other drug used in hyperthyroidism preoperative preparation,which was rapid,safe and effective,but it can lead to hypokalemia complication,especial in the 24 hours after the first use,should be brought clinical doctors attentions,and give timely diagnosis and treatment.

OBJECTIVE To explore the positive rate of intercellular adhesion (ica) operon and its production- polysaccharide intercellular adhesion(PIA) and the relationship between the rate and adhesion of Staphylococcus epidermidis. METHODS Amplifying icaA of ica operon by PCR and then detecting it by agarose electrophoresis, detecting PIA by Congo red agar (CRA) to detect its adhesive ability by infusion set. RESULTS The icaA positive rate of S.epidermidis was 51.5%; the PIA positive rate was 50%; there was significant difference between the clone numbers of PIA positives and negatives (P

OBJECTIVE:To determine the resistance of clinically isolated bacteria to meropenem METHODS:The resistance of 150 clinically isolated strains to meropenem and several other antibiotics was detected by K-B method and the drug resistant spectra to E coli were compared between meropenem and imipenem RESULTS:The sensitivity of 130 clinical isolates to meropenem was 100% No cross resistant strains of E coli was found to meropenem and imipenem CONCLUSION:Meropenem is highly sensitive to the clinical isolates,and its antibacterial spectrum is similar to that of imipenem

Aim To investigate the changes of plasticity and distribution in the lungs of asthmatic guinea pigs.Methods Guinea pigs were divided into asthma group and control group.Immunohistochemistry was used in this study to observe the changes of ERK1/2 and Elk phosphoryiation in the lungs of guinea pigs.Results There were significant changes in distribution of EPK1/2 and Elk in the lungs of asthma group.The positive cells were detected on the walls of respiratory bronchioles and alveolar ducts,especially on the smooth muscle layer and basement membrane.In addition,the numbers of positive cell were clearly increased in asthma group(P< 0.01). Conclusion There is close relationship between the EPK1/2 and Elk phosphoryiation and attack of asthma.It may be a reason for persistence and progress of asthma.

Objective To determine whether ultrasound (US) exposure combined with microbubble destruction could be used to enhance non-viral gene delivery in rat C6 glioma cells. Methods Microbubbles were prepared and gently mixed with plasmid DNA. The mixture of the DNA and microbubbles was administered to cultured C6 cells under different US/microbubble conditions. US parameters adopted in this study were frequency 1 MHz, output intensity 1 W/cm2, duty cycle 20%, exposure time 30 seconds. Transfection efficiency and cell viability were assessed by FACS analysis, confocal laser scanning microscopy, and Try pan blue staining. Results Microbubble with US exposure could significantly enhance the reporter gene expression as compared with other groups. No statistical significant difference was observed in the glioma cell viability between different groups. Conclusions US-mediated microbubble destruction has the potential to promote safe and efficient gene transfer into C6 cells,and it may be useful for safe clinical gene therapy of brain cancer without a viral vector system.

Objective To investigate expression,significance and relationship of IMP3 and CD44v6 protein in papillary thyroid carcinoma.Methods Immunohistochemical method was used to investigate the expression of IMP3 and CD44v6 protein in 30 cases PTC(10 follicular variant of papillary carcinoma cases,20 conventional papillary thyroid carcinoma cases),20 PTC with lymph node metastasis cases,20 benign tissues of thyroid cases.Quantitative analysis of the detection was conducted by Biosens Digital Imaging System vl.6 (professional image analysis software),and the target integral optical density (IOD) was used for the judgement parameter.Results IMP3 and CD44v6 expression in the PTC is higher than in benign group,the difference was statistically significant (P ＜ 0.05) ; IMP3 and CD44v6 expression in PTC with lymph node metastasis was higher than without lymph node metastasis in the organization to express,the difference was statistically significant (P ＜ 0.05) ; There was a positive correlation between IMP3 and CD44v6 expression and metastasis in PTC (r =0.903,P ＜ 0.05).Conclusions The expression of IMP3 and CD44v6 protein was closely correlated with the invasion and metastasis of PTC.IMP3 and CD44v6 protein might be considered as molecular markers for PTC.

Objective To investigate the design and manufacture of 3D printed compensator in electron radiation therapy for Merkel cell carcinoma,and to verify the feasibility of this technique in electron radiation therapy.Methods Computed tomography was used to collect images of a human head phantom.The delineation of target volume of Merkel cell carcinoma was simulated in the planning system and a radiotherapy plan was formulated after adding the compensator.The compensator was printed out by a 3D printer and fixed on the head phantom.A second CT scan was performed to make a new treatment plan.For the two plans,several planes parallel to the beam were selected to calculate gamma passing rates.The actual dose distribution was measured using disposable films.The gamma passing rate was compared between the film system and the planning system.The conformity index (CI) and the heterogeneity index (HI) of target volume were compared between the plans using the printed compensator and the conventional compensator of the same thickness.Comparison between the two plans was made by paired t test.Results Using the dose distribution of the plan with simulated compensator,the gamma passing rate was 94.7±2.3％ in the plan with 3D printed compensator.Using the dose distribution measured by the film,the gamma passing rate was 96.6％ in the plan with 3D printed compensator.Compared with the conventional compensator,the 3D printed compensator achieved a significantly elevated CI (0.85 vs.0.69,P=0.004) and a slightly improved HI (1.30 vs.1.26,P=0.001).Conclusions The conformal dose distribution provided by 3D printed compensator for tumors at different depths meets the clinical need.