1.An Epidemiological Study of Female Incontinence.
Journal of the Korean Continence Society 1997;1(1):55-55
No abstract available.
Epidemiologic Studies*
;
Female
;
Humans
2.Distribution Pattern of Prostatic Weight and Proposal of Its Normal Range.
Hyuk June LEE ; Moon Kee CHUNG ; Choong Rak KIM
Korean Journal of Urology 2000;41(1):59-64
No abstract available.
Reference Values*
3.Multilocular Renal Cyst: Report of Two Cases.
Hyuk June LEE ; Sang Don LEE ; Nam Cheol PARK
Korean Journal of Urology 1996;37(11):1313-1318
Multilocular cyst of the kidney is a rare pathologic entity, noninherited benign renal neoplasm occurring in the both children and adults. The entities commonly were known as multilocular cyst of the kidney or multilocular cystic nephroma. The controversy over the pathogenesis of multilocular cyst-neoplasm versus developmental anomaly- is still unsettled. We report two cases of multilocular renal cyst, one in a 8-year-old child with a chief complaint of gross hematuria and another in a 44-year-old woman with epigastric discomfortness. They were preoperatively suspected multilocular cyst by ultrasonography and computed tomography. We performed a simple nephrectomy as the treatment of choice because of the difficulty in accurate diagnosis and the documented association with renal neoplasia.
Adult
;
Child
;
Diagnosis
;
Female
;
Hematuria
;
Humans
;
Kidney
;
Kidney Neoplasms
;
Nephrectomy
;
Ultrasonography
4.Altered expression of potassium channel genes in familial hypokalemic periodic paralysis
June-Bum Kim ; Gyung-Min Lee ; Sung-Jo Kim ; Dong-Ho Yoon ; Young-Hyuk Lee
Neurology Asia 2011;16(3):205-210
We analyzed the mRNA expression patterns of major potassium channel genes to determine the
mechanism of hypokalemia in familial hypokalemic periodic paralysis. We used quantitative RT-PCR
to examine the mRNA levels of both inward (KCNJ2, KCNJ6, and KCNJ14) and delayed rectifi er
(KCNQ1 and KCNA2) potassium channel genes in skeletal muscle cells from both normal and patient
groups, prior to and after exposure to 4 mM and 50 mM potassium buffers. Quantitative RT-PCR
analysis revealed no changes in the mRNA levels of these genes in normal and patient cells on exposure
to 4 mM potassium buffer. However, after exposure to 50 mM potassium buffer, which was used to
induce depolarization, normal cells showed a signifi cant decrease in KCNJ2, KCNJ6, and KCNJ14
expression, but no change in KCNQ1 and KCNA2 expression. In contrast, patient cells showed no
change in KCNJ2 and KCNJ6 expression, but an increase in KCNJ14 expression. Furthermore, KCNQ1
and KCNA2 showed decreased expression. We found that the expression levels of both inward and
delayed rectifi er potassium channel genes in patient cells differ from those in normal cells. Altered
potassium channel gene expression in patient cells may suggest a possible mechanism for hypokalemia
in familial hypokalemic periodic paralysis.
5.Analysis of Chromosomal Abnormality in Male Infertility.
Hyuk June LEE ; Nam Cheol PARK
Korean Journal of Urology 1998;39(4):396-402
PURPOSE: Chromosomal abnormality is the major cause of male infertility. We evaluated the usefulness of chromosome analysis for searching the etiology of male infertility. MATERIALS AND METHODS: Karyotypes of 375 cases with male infertility except postvasectomy infertility were analyzed. The comparison between sexchromosomal abnormality and autosomal abnormality in various clinical parameters, serum hormonal levels and sperm density was done. RESULTS: Of 375 cases, 57 cases(15.2%) of chromosomal abnormalities were found, consisting of 42 cases(73.7%) of sex chromosomal abnormality(38 Klinefelter's syndrome with mosaicism, 1 sex reverse syndrome, 1 true hermaphroditism, 1 Y chromosome deletion, and 1 sex-chromosome translocation with autosome), 13 cases(22.8%) of autosomal abnormality(7 translocation and 6 inversion) and 2 cases(3.5%) of normal variant. Of various clinical parameters and serum hormonal levels, only testicular volume and serum testosterone level revealed significant statistical difference between sex chromosomal and autosomal abnormality. CONCLUSIONS: We suggest that the chromosomal analysis should be the first line aid in searching etiology of the male infertility.
Chromosome Aberrations*
;
Humans
;
Infertility
;
Infertility, Male*
;
Karyotype
;
Klinefelter Syndrome
;
Male
;
Male*
;
Mosaicism
;
Ovotesticular Disorders of Sex Development
;
Spermatozoa
;
Testosterone
;
Y Chromosome
6.Novel Drugs for Asthma Treatment: Immunomodulatory Therapy.
Korean Journal of Medicine 2012;83(2):190-196
The management of asthma focuses on the reduction of airway inflammation accompany with symptomatic care after recognition. Glucocorticosteroid is the most important drug to reduce airway inflammation, and it has been used inhaled, orally and systemically. New knowledge about the pathogenesis of allergy and asthma has made the development and clinical trial of target or immunomodulator therapy. It includes cytokine, cytokine blockers, specific cytokine receptor blocker, and immunostimulatory oligodeoxynucleotides. These agents are thought to hold the promise for more beneficial outcomes in the future, although it showed limited therapeutic benefits only for patients, especially intractable or severe asthma, until now.
Asthma
;
Humans
;
Hypersensitivity
;
Immunomodulation
;
Inflammation
;
Oligodeoxyribonucleotides
;
Receptors, Cytokine
7.Application of Medifoam B(R) & Negative Pressure Therapy for the Auxiliary Treatment of Pressure Sore.
Journal of the Korean Society of Plastic and Reconstructive Surgeons 2004;31(5):733-736
Pressure sore is usually developed by repeated or persistent pressure given to fixed regions. Due to increase in old ages, paraplegia or quadriplegia by traffic accidents and industrial accidents, the incidence of pressure sore is increasing. V.A.C.(R)(vacuum assisted closure, KCI Inc, U.S.A.) has been highly effective for accelerating wound healing, however, high cost and the use of special equipment have been preventing it from popular use. We applied the negative pressure of wall suction (100-150mmHg) with Medifoam B(R)(Hydrophilic polyurethane foam, Ildong Pharm, Co., Korea) on pressure sore wound instead of expensive dressing foam of V.A.C.(R) So, we could accelerate wound healing without special equipment or incurring high cost. We were able to reduce the cost to 10% with Medifoam B(R) and wall suction dressing compared with V.A.C.(R) In this aspect, the technique explained above seems to be clinically useful.
Accidents, Occupational
;
Accidents, Traffic
;
Bandages
;
Incidence
;
Paraplegia
;
Polyurethanes
;
Pressure Ulcer*
;
Quadriplegia
;
Suction
;
Wound Healing
;
Wounds and Injuries
8.Gene - Gene Interactions Among MCP Genes Polymorphisms in Asthma.
June Hyuk LEE ; Choon Sik PARK
Allergy, Asthma & Immunology Research 2014;6(4):333-340
PURPOSE: Monocyte chemoattractant proteins (MCPs) are important cytokines that involved in cellular activation and releasing of inflammatoy mediators by basophils and eosinophils in allergic disease. Some MCP gene variants implicate in asthma and monoclonal antibody for MCP-3 blocks allergic inflammations in the patients with asthma. Detection of interactions between gene and environment or between genes for complex disease such as asthma is important. We searched for an evidence of genetic effect of single nucleotide polymorphisms (SNPs) of MCP genes as well as gene - gene interactions involved in asthma. METHODS: Four hundreds asthmatics and four hundreds normal controls were enrolled. Asthma was defined as a positive bronchodilator response or positive methacholine provocation test with compatible clinical symptoms. Seven MCP gene SNPs (2 SNPs in MCP-1, 1 in MCP-2, and 4 in MCP-3) were included. Association analyses between SNP and asthma, and the tests for gene - gene interaction were performed. RESULTS: Strong linkage disequilibria were found among 7 MCP gene polymorphisms. There was no SNP that showed a significant association with asthma among 7 SNPs of 3 MCP genes. No haplotype was associated with asthma, either. The combination of MCP1-2518G>A, MCP2+46A>C, and MCP3+563C>T was the best predictive model for asthma as compared to the control in tests for gene - gene interaction. The MCP1-2518G>A and MCP2+46A>C was the second best predictive combination and this had the highest synergistic interaction effect on the subject's status than any other combination of polymorphisms. Complete linkages were not associated with the gene - gene interactions models. CONCLUSIONS: MCP gene polymorphisms probably interact with each other; thus, these findings may help in developing a possible genetic marker to predict asthma.
Asthma*
;
Basophils
;
Cytokines
;
Eosinophils
;
Genetic Markers
;
Haplotypes
;
Humans
;
Inflammation
;
Methacholine Chloride
;
Monocyte Chemoattractant Proteins
;
Polymorphism, Single Nucleotide
9.Gene - Gene Interactions Among MCP Genes Polymorphisms in Asthma.
June Hyuk LEE ; Choon Sik PARK
Allergy, Asthma & Immunology Research 2014;6(4):333-340
PURPOSE: Monocyte chemoattractant proteins (MCPs) are important cytokines that involved in cellular activation and releasing of inflammatoy mediators by basophils and eosinophils in allergic disease. Some MCP gene variants implicate in asthma and monoclonal antibody for MCP-3 blocks allergic inflammations in the patients with asthma. Detection of interactions between gene and environment or between genes for complex disease such as asthma is important. We searched for an evidence of genetic effect of single nucleotide polymorphisms (SNPs) of MCP genes as well as gene - gene interactions involved in asthma. METHODS: Four hundreds asthmatics and four hundreds normal controls were enrolled. Asthma was defined as a positive bronchodilator response or positive methacholine provocation test with compatible clinical symptoms. Seven MCP gene SNPs (2 SNPs in MCP-1, 1 in MCP-2, and 4 in MCP-3) were included. Association analyses between SNP and asthma, and the tests for gene - gene interaction were performed. RESULTS: Strong linkage disequilibria were found among 7 MCP gene polymorphisms. There was no SNP that showed a significant association with asthma among 7 SNPs of 3 MCP genes. No haplotype was associated with asthma, either. The combination of MCP1-2518G>A, MCP2+46A>C, and MCP3+563C>T was the best predictive model for asthma as compared to the control in tests for gene - gene interaction. The MCP1-2518G>A and MCP2+46A>C was the second best predictive combination and this had the highest synergistic interaction effect on the subject's status than any other combination of polymorphisms. Complete linkages were not associated with the gene - gene interactions models. CONCLUSIONS: MCP gene polymorphisms probably interact with each other; thus, these findings may help in developing a possible genetic marker to predict asthma.
Asthma*
;
Basophils
;
Cytokines
;
Eosinophils
;
Genetic Markers
;
Haplotypes
;
Humans
;
Inflammation
;
Methacholine Chloride
;
Monocyte Chemoattractant Proteins
;
Polymorphism, Single Nucleotide
10.Deducing Isoform Abundance from Exon Junction Microarray.
Po Ra KIM ; S June OH ; Sang Hyuk LEE
Genomics & Informatics 2006;4(1):33-39
Alternative splicing (AS) is an important mechanism of producing transcriptome diversity and microarray techniques are being used increasingly to monitor the splice variants. There exist three types of microarrays interrogating AS events-junction, exon, and tiling arrays. Junction probes have the advantage of monitoring the splice site directly. Johnson et al., performed a genome-wide survey of human alternative pre-mRNA splicing with exon junction microarrays (Science 302:2141-2144, 2003), which monitored splicing at every known exon-exon junctions for more than 10,000 multi-exon human genes in 52 tissues and cell lines. Here, we describe an algorithm to deduce the relative concentration of isoforms from the junction array data. Non-negative Matrix Factorization (NMF) is applied to obtain the transcript structure inferred from the expression data. Then we choose the transcript models consistent with the ECgene model of alternative splicing which is based on mRNA and EST alignment. The probe-transcript matrix is constructed using the NMF-consistent ECgene transcripts, and the isoform abundance is deduced from the non-negative least squares (NNLS) fitting of experimental data. Our method can be easily extended to other types of microarrays with exon or junction probes.
Alternative Splicing
;
Cell Line
;
Exons*
;
Humans
;
Least-Squares Analysis
;
Protein Isoforms
;
RNA Precursors
;
RNA, Messenger
;
Transcriptome