1.Generation and immunogenicity evaluation of Senecavirus A virus-like particles.
Chunping WU ; Yi RU ; Hong TIAN ; Kun MA ; Rongzeng HAO ; Yajun LI ; Juncong LUO ; Zhengwang SHI ; Huanan LIU ; Zhi ZUO ; Haixue ZHENG
Chinese Journal of Biotechnology 2021;37(9):3211-3220
To develop Senecavirus A (SVA) virus-like particles (VLPs), a recombinant prokaryotic expression plasmid pET28a-SVA-VP031 was constructed to co-express SVA structural proteins VP0, VP3 and VP1, according to the genomic sequence of the field isolate CH-FJ-2017 after the recombinant proteins were expressed in E .coli system, and purified by Ni+ ion chromatographic method. The SVA VLPs self-assemble with a high yield in vitro buffer. A typical VLPs with an average diameter of 25-30 nm which is similar to native virions by using TEM detection. Animals immunized by SVA VLPs shown that the VLPs induced high titers neutralizing antibodies in Guinea pigs. This study indicated that the VLPs produced with co-expressing SVA structural proteins VP0, VP3 and VP1 in prokaryotic system is a promising candidate and laid an important foundation for the development of a novel SVA VLPs vaccine.
Animals
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Antibodies, Neutralizing
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Escherichia coli/genetics*
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Genomics
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Guinea Pigs
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Picornaviridae/genetics*
Result Analysis
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