Objective To analyze the etiological and epidemiological characteristics of hand-foot-mouth disease(HFMD) in Liuzhou city from 2013 to 2016,and to provide scientific evidence for preventive policy making.Methods Specimens of HFMD cases were collected from the hospitals and county CDC from 2013 to 2016.The nucleic acids which are enterovirus viruses71(EV71),coxasckie virus A16(CoxA16) and other enterovirus(EV) was detected by Real time PCR technique.Results A total of 3 503 suspected HFMD cases were found,and 2 720 cases were identified.The main pathogen of HFMD in Liuzhou city was change every year from 2013 to 2016,in addition to EV71 and CoxA16,there was a higher proportion of EV.HFMD was a seasonal disease,most cases occurred from March to September.The children under 5 years old,especially 1-3 years old were the main patients,and male patients was higher than female.Severe and death HFMD was EV71,but death HFMD had no CoxA16.Conclusion EV was the main pathogen of mild HFMD in Liuzhou city from 2013 to 2016,but the pathogenic spectrum was changing every yeas.EV71 was the main pathogen of severe and death HFMD.

Objective:To explore a new method of the cultivation of adoptive immunotherapy cells.Methods: Mononuclear cells was isoplated by density gradient centrifugation and then proliferated by using CD 40-agonist monoclonal antibody 5C11、cytokine of IFN-αand IL-7(CD40 group)in vitro.During the culturing procedure ,the cell morphology was obersved by optical microscope.The percentage of T-lymphocytes, NK-T cells, Treg cells and the cell proliferation, which were compared with CIK group CD3mAb activated,was detected on the 9th day.Results:There was no significant difference of CD 4+/CD8+T cells percentage between the two groups.But the Treg cells percentage of CIK group was far higher than that of CD 40 group,while the percentage of CD3+CD56+NK-T cells was lower than that of CD 40 group.And a group of Mo-NK-DC cells were observed in the CD 40 group.Conclusion: The new method of adoptive immunity therapy has been established in this study could increase the percentage of NK -T cells which had the ability to kill tumor cells.Simultaneously ,it is reduced the amount of Treg cells significantly.

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