[Objective]To establish an animal model of heterotopie ossification(HO)induced by Achilles' tenotomy in Sprague-Dawley(SD)rat and to investigate its possible mechanism.[Method]Thirty six male SD rats were divided into 3 groups(n=12).In sham group,incision and suturation were performed only in the left leg.Partial tenotomy was performed in the left Achilles tendons in PAT group.In AT group,tenotomy was performed in the left Achilles tendons to establish animal model of HO.X-ray and histological examinations were made at 6 and 10 weeks after operation.[Result](1)No HO occurred in the sham and PAT groups.(2)In AT group,3 rats showed cartilage and bone formation on X-rays while the remaining 3 rats showed chondrification in histological examination at 6 weeks after operation.At 10 weeks all rats showed bone formation with trabecular bone.This kind of HO developed through a process of endochondral ossification.[Conclusion]Tenotomy is a simple,effective and feasible method to induce HO.

Objective To compare the curative effects of multi-level puncture versus single-level puncture percutaneous vertebroplasty(PVP) for treatment of multiple-level osteoporotic vertebral body compression fractures(OVCF) in senile patients.Methods From June 2008 through November 2014,89 senile patients with fresh multiple-level OVCF underwent PVP guided by C-arm fluoroscopy in prone position.Of them,51 received PVP in which the vertebrae of multiple levels were simultaneously punctured for bone cement injection while the other 38 received PVP in which the vertebrae of multiple levels were punctured one by one for bone cement injection.The 2 groups were compatible with no significant differences in preoperative demographic data (P ＞ 0.05).The 2 groups were compared in terms of visual analgesic scale (VAS),operation time for a single vertebra,fluoroscopy times for a single vertebra,bone cement injection amount for a single vertebra,and extraosseous cement leakage.Results PVP procedures were successful in both groups without serious complications.The VAS scores in both groups at 2 days post-operation were significantly lower than those at pre-operation(P ＜ 0.05).The operation time and fluoroscopy times for a single vertebra in the multi-level puncture PVP group were significantly less than those in the single-level puncture PVP group (P ＜ 0.05).There were no significant differences between the 2 groups in bone cement injection amount for a single vertebra or extraosseous cement leakage (P ＞ 0.05).Conclusions The curative effects of multi-level puncture and single-level PVP are satisfactory for senile OVCF,but multi-level puncture PVP may lead to less operation time and less X-ray exposure.

AIM: To investigate the effect of heme oxygenase /carbon monoxide(HO/CO) system on the pathogenesis of hypertension and effect of Heme-L- Lysinate(HLL), Zine protoporphyrin-IX(ZnPPIX) on vascular tension in a two kidney one-clip model. METHODS: The changes in mean arterial pressure and heart rate as well as phenylephrine(PHE)-induced contraction in isolated aortic rings of HLL, ZnPPIX- treated rats were examined after intraperitoneal administration of HLL and carbon monoxide( CO). RESULTS: (1) Injection of exogenous CO resulted in a significant decrease in mean arterial pressure in Sham and 2K1C groups(P

This is an in vitro study to explore the effects of cryopreservation and resuscitation on the biological characteristics of osteoblasts at different times. Osteoblasts taken from the crania of newly born SD rats were cultured. Comparative studies were made on the cells' proliferation, the activity of alkaline phosphatase (ALP), and the number of live cells among fresh cultured osteoblasts and cells after the inception of cryopreservation and resuscitation at time-periods of one, three, six months respectively. The results showed that there were no significant differences among four groups in cell proliferation and in activity of ALP (P > 0.05). Yet, after cryopreservation and resuscitation, there were significant differences between the six-month group and the other three groups (P < 0.05). The results also showed, after cryopreservation and resuscitation, there were no significant differences between the control group and the one-month and three-month groups, respectively (P > 0.05). These findings indicated that the live cells might decrease in number after the osteoblasts were cryopreserved for too long a period, but after cryopreservation and resuscitation, the cells still retained the original biological characteristics of osteoblasts.
Alkaline Phosphatase ; metabolism ; Animals ; Animals, Newborn ; Cell Proliferation ; Cells, Cultured ; Cryopreservation ; methods ; Osteoblasts ; cytology ; Rats ; Rats, Sprague-Dawley ; Skull ; cytology ; Time Factors ; Tissue Engineering ; methods